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정해원,최진영,이원영 대한보건협회 1988 대한보건연구 Vol.14 No.1
Drug resistance of P 815 mouse tumor cells after various drug treatment were studied "by scoring the changes of cell sizes. Percent of cells with abnormal large cell size (larger than 15㎛. in diameter) increased by treatment of anticancer drugs. When the cells were treated with adriamycin, cells larger than 15㎛ in diameter were observed at second day post-treatment and the percent of larger cells reached maximum at 9th day post-treatment. Similar trends were observed following the treatment with cisplatin, 5-Fu, or methotrexate. Inverse relationship between survival of tumor cell population and percent distribution of large cells was observed when cisplatin was treated. Thus it was suggested that co-existence of abnormal large cells and cells in original size in the tumor cell population after drug treatment might be a key to determine that the cell line is resistant which thereby indication for next drug of choice.
정해원,김수영,하성환,김태환,조철구 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.2
본 연구는 염색체 1, 2, 4, 7, 8, 9 및 21번 염색체의 DNA probe를 이용하여 2Gy의 방사선을 조사한 후 DNA 양을 감안한 기대치와 관찰치의 차이를 비교함으로서 각 염색체의 방사선에 대한 감수성을 평가하여 궁극적으로 방사선 피폭시 생물학적 선량계로서 FISH기법의 타당성을 평가하고자 하였다. 1번 및 4번 염색체의 경우 상호전좌와 이동원 염색체의 관찰치가 기대치보다 더 높게 나타났으며 이와 반대로 2, 7, 8 및 9번 염색체의 경우 상호전좌와 이동원 염색체의 관찰치 모두 기대치보다 낮게 나타났다. 2번 및 4번 염색체의 경우 1번 염색체보다 더 많은 acentric fragment의 빈도를 나타내었다. 1, 2, 및 4번 염색체 3종을 조합했을 때 상호전좌의 경우 관찰치와 기대치는 세포 100개당 25.5 및 25.40으로 차이가 없었으며 이동원염색체의 경우 13.25 및 13.2로 역시 거의 차이가 없게 나타났다. 따라서 본 연구결과 방사선 피폭시 발생하는 염색체이상 빈도는 염색체마다 DNA양에 비례해 나타나지 않을 수 있어 각 염색체마다 방사선 감수성에 차이가 있을 것으로 판단된다. 또한 방사선 피폭시 생물학적 선량계로서 1, 2 및 4번을 동시에 관찰 할 경우 염색체 FISH기법을 활용하기 위하여 적절한 염색체 조합이라고 판단된다. To study the relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural chromosome aberrations, the frequencies of translocations and dicentrics induced in human lymphocytes after in vitro exposure to 2Gy of x-ray were analysed by fluorescene in situ hybridication(FISH). Single whole chromosome probes(WCP), specific for chromosomes 1, 2, 4, 7, 8, 9, and 21 and triple combination of probes for chromosome 1, 2 & 4 were used separately. A significant deviation of the frequency of symmetrical translocation and dicentrics from a DNA-proportional distribution was observed. Chromosomes 2, 7, 8, 9 and 21 were less frequently involved in the formation of symmetrical translocations and dicentrics that expected, whereas chromosomes 1 and 4 were more frequently involved. Chromosome 2 and 4 showed a higher frequency of acentric fragments. When triple combination probe for chromosome 1, 2 & 4 was used, no differences were found between the observed and expected frequency of exchange type aberrations. The results showed that the frequency of radiation-induced chromosome aberrations was not proportional to DNA contents, suggesting the difference in the susceptibility to specific aberrations among individual chromosomes. The results also indicated that the FISH technique with combination of probes for chromosome 1, 2 & 4 was useful for radiation biodosimetry.
적정기술을 통해 교육사업하기 : - ‘마을기술센터 핸즈'의 7년 운영사례 -
정해원 한밭대학교 적정기술연구소 2021 적정기술 Vol.13 No.1
마을기술센터 핸즈(이하 ‘핸즈)는 2014년 적정기술을 교육하고 연구하며 컨설팅하는 회사로 창업해서 2020년 현재 운영중이다. 자영업자로서 2020년 초 코로나19가 확산하며 잠시 전망이 불투명한 적도 있었지만, 지금은 비대면 온라인교육과 납품 등으로 살아가는 법을 익히고 있다. 이 글은 적정기술 교육기업 핸즈를 7년 동안 운영하며 겪고 고민했던 지점들을 기록한 것이다.
정해원,유영수 한국환경보건학회 1982 한국환경보건학회지 Vol.8 No.1
Ethylene oxide gas has been used as a cold sterilant for heat-sensitive medical equipments and as a fumigant for food for more than 30 years, and it is used more widely than radiation although radiation sterilization has made significant inroads in recent years. But according to recent studies of toxicities such as mutagenicity, haemolytic effect and possible carcinogenicity of Ethylene oxide (ETO) and its two main reaction products, Ethylene chlorohydrin (ETCH) and Ethylene glycol (ETG), Environmental Protection Agency in U.S.A. has suggested some regulations on residual gas in drug products and medical devices for human use. The mutagenic activity of ETO compared with that of X-ray has an equivalency of 1 ppm/hr for ETO as compared to 20 mrad for X-ray, and one could suggest the present maximum allowable concentration for ETO (50 ppm) should be 400 times lower than the radiation standard (2.5 mrad/hr). Although radiation sterilization has advantages of simplicity of operation and complete reliability, changes of physico-chemical properties with possible formation of toxic substances may occur. It is therefore necessary to make some regulations of our own for residual toxicities orginated from each sterilization method.
유기물 바인더를 사용한 $YBa_2Cu_3O_{7-$\delta$}$ 초전도 Coil의 제조
정해원,박승만,김재묵,김성수 한국세라믹학회 1990 한국세라믹학회지 Vol.27 No.3
One of the possible ways to make a flexible wire of high-Tc superconductiong ceramics is the extrusion of a mixture slurry of superconducting powder with an appropriate polymer binder. The fabrication procedure for YBa2Cu3O7-$\delta$ superconducting coils with this plastic mass is described. The major factors limiting the formation of extruded wire are the binder content, powder size, and entrapped gas in the mixture slurries. The optimum content of binder for both good flexbility and strength of wire was estimated to be 30wt%. The finer the powder size is, the more homogeneous structure the extruded wire has. The vacuum degassing before extrusion was necessary to remove the entrapped gas in as-extruded wire. The formability of wire depends greatly on the wire radius and binder content. After burning out the binder and the successive sintering, the contacts between the superconducting grains could be made. The resistivity vs. temperature behavior measured in the final wire showed the transition temperature of 90K with narrow transition width. However, the critical current densities of these wires are much lower in comparison to those of conventional bulk specimens.