Telomere의 기능 손실에 의한 Telomerase 결핍 세포의 성장 억제에 대한 연구

주연진,박정은,전경미,김창민,김상훈,박원봉,이기호 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.3

불소인식정도에 대한 조사보고

김미라,백병주,김재곤,이승익,양연미 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.4

저자는 1998년 6월과 7월에 전북대학교병원 소아치과에 내원한 초진환자중 설문지에 응답할 수 있는 환자 50여명과 보호자 100명을 대상으로 불소에 대해 어느정도 인식하고 있는지 알아보고자 설문지를 이용하여 조사하여 다음과 같은 결론을 얻었다. 1. 불소의 치과에서의 사용여부에 대한 인식정도는 환자 26%, 보호자 51%, 전체적으로 42.7%를 보였다. 2. 불소의 우식예방효과에 대한 인식정도는 환자 86%, 보호자 85%, 전체적으로 85.7%의 높은 비율을 보였다. 3. 이에 반해 불소도포희망 여부에 대하여 환자 46%, 보호자 44%만이 긍정적으로 답하였다. 4. 불소를 이용하거나 도포한 경험은 환자 60%, 보호자 55%로 낮은 비율을 보였다. 5. 불소의 우식예방효과에 대한 인식여부와 실제이용 정도는 일치하지 않았다. 이에 치과 종사자 및 대중매체를 통해 보다 적극적인 불소사용의 교육이 이루어진다면 불소이용은 증가할 것이다. At the department of Pediatric Dentistry in Chonbuk National University Hospital, authors conducted a survey of 50 new patients and 100 new parents regarding fluoride in order to discover what they think about fluoride. The results obtained were as follows ; 1. It answered patients 26%, parents 51% and total 42.7% in knowledge about the use of fluoride in dental clinics. 2. The knowledge about caries prevention effect of fluoride is high ; patients 86%, parents 85% and total 85.3% 3. The patients and parents who wanted to use fluoride are 46%, 44% respectively 4. The patients and parents who experienced fluoride are 60%, 55% respectively 5. It is not correspond the knowledge about caries prevention effect of fluoride and the practical method of application These results suggest that the number of patients and parents who use fluoride will increase if we advise them adequately

다량의 하부위장관출혈이 병발된 Vibrio vulnificus 패혈증 1예

최주연,유진홍,한석원,이대훈,최민호,박순민,김연식,김선우,신완식,강문원,강성구 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.2

최근 저자들은 Vibrio vulnificus 감염에 의해 패혈증 및 이차성 피부 병변을 보인 42세의 남자 환자의 치료중 하부 위장관 출혈이 병발된 1예를 경험하였기에 보고하는 바이다. We experienced a case of Vibrop vulnificus septicemia which showed an unusual manifestation such as hematochezia. The patient was a 42-year-old man with chronic liver disease. The blood and wound culture revealed the organism. During intensive care, he suffered from massive hematochezi for several days. Colonoscopy and abdominal CT showed findings suspicious of ischemic colitis. To our knowledge, this is the first unusual case of V.vulnificus infection which showed massive lower gastrointestinal bleeding.

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

산국의 수량과 유효성분에 대한 NPK의 효과

이경동,양민석,정연규,손보균,조주식,이성태,김필주 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.2

본 연구는 3요소(N-PO_2O_5-K_2O)처리가 산국(Chysanthemum boreale M.)의 수량과 유효성분에 미치는 효과를 조사하여 효과적인 재배시스템을 개발하고자하였다. 처리구는 무처리, NPK(250-160-160), NK(250-0-160), PK(0-160-160kg/ha)의 5처리로 설정하여 산국을 재배한 결과, 건물수량은 NPK처리구가 PK처리구에 비하여 4.0배의 증수가 있었으며, 다른 처리구보다 무기이온의 흡수율, 생육 및 수량에서 NPK처리구가 가장 우수하였다. 식용으로 많이 쓰이는 꽃의 주요 아미노산은 proline, glutamic acid과 aspartic acid이였고, 다른 처리구들에 비하여 NPK처리구가 높은 함량을 보였다. 혈압강하효과가 우수한 cumambrin A는 PK처리구에 비하여 NPK처리구가 6.2배의 증수효과가 있었다. NK처리구는 NP처리구보다 cumambrin A의함량을 높이기 위해서는 인산의 시용보다 칼리의 시용이 더 요구되며, 야외포장에서의 인산과 칼리의 적정 시비량의 설정이 필요하다고 판단된다. To establish the fertilization condition to increase the productivity of Chrysanthemum boreale M. with high quality, the effects of three nutrients (N, P, K) on the yields and the effective components were investigated in the pot scale. NPK was applied by chemical fertilizers with N-P_2O_5-K_2O = 250-160-160 kg/ha as a main treatment, and NP (N-P_2O_5 = 250-160 kg/ha), NK (N-KO = 250-160 kg/ha), and PK (PO_2-KO_5 = 160-160 kg/ha) treatments were settled as comparison. Dry yields of C. boreale M. was increased significantly to 4 fold higher by nitrogen. Nitrogen increased apparently plant growth and inorganic nutrient uptake. In the flower, which is most useful and edible part as a herbal medicine, main amino acids were glutamic acid and aspartic acids, and the total content was increased significantly by three elements of application. In addition, the content of cumambrin A, which is known to have the effect of blood-pressure reduction, was increased source to 6.2 times by nitrogen higher than that in PK treatment. Potassium was more effective in biosyndiesis of cumatnbrin A than phosphorus, but the biological pathway was not clear, still.

Original Article : Impact of immunosuppressant therapy on early recurrence of hepatocellular carcinoma after liver transplantation

( Ju Yeun Lee ),( Yul Hee Kim ),( Nam Joon Yi ),( Hyang Sook Kim ),( Hye Suk Lee ),( Byung Koo Lee ),( Hye Young Kim ),( Young Rok Choi ),( Geun Hong ),( Kwang Woong Lee ),( Yung Suk Suh4 ) 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.2

Background/Aims: The most commonly used immunosuppressant therapy after liver transplantation (LT) is a combination of tacrolimus and steroid. Basiliximab induction has recently been introduced; however, the most appropriate immunosuppression for hepatocellular carcinoma (HCC) patients after LT is still debated. Methods: Ninety-three LT recipients with HCC who took tacrolimus and steroids as major immunosuppressants were included. Induction with basiliximab was implemented in 43 patients (46.2%). Mycophenolate mofetil (MMF) was added to reduce the tacrolimus dosage (n=28, 30.1%). The 1-year tacrolimus exposure level was 7.2 ± 1.3 ng/mL (mean ± SD). Results: The 1- and 3-year recurrence rates of HCC were 12.9% and 19.4%, respectively. Tacrolimus exposure, cumulative steroid dosages, and MMF dosages had no impact on HCC recurrence. Induction therapy with basiliximab, high alpha fetoprotein (AFP; >400 ng/mL) and protein induced by vitamin K absence/antagonist-II (PIVKA-II; >100 mAU/mL) levels, and microvascular invasion were significant risk factors for 1-year recurrence (P<0.05). High AFP and PIVKA-II levels, and positive 18fluoro-2-deoxy-d-glucose positron-emission tomography findings were significantly associated with 3-year recurrence (P<0.05). Conclusions: Induction therapy with basiliximab, a strong immunosuppressant, may have a negative impact with respect to early HCC recurrence (i.e., within 1 year) in high-risk patients. (Clin Mol Hepatol 2014;20:192-203)

Effects of Gamisoyosan on In Vitro Fertilization and Ovulation of Stressed Mice by Electric Shock

Kim, Ji-Yeun,Kwak, Dong-Hoon,Ju, Eun-Jin,Kim, Sung-Min,Lee, Dae-Hoon,Keum, Kyung-Su,Lee, Seo-Ul,Jung, Kyu-Yong,Seo, Byoung-Bu,Choo, Young-Kug The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.11

Exposure to stress is known to precipitate or exacerbate many reproductive dysfunctions such as dysmenorrhea and infertility. Abnormalities of the reproductive system, as shown by reduced ovulation, fertilization and early embryonic development, are frequently seen in dysmenorrhea and infertility. It has been generally accepted that Gamisoyosan (GSS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also GSS has been used traditionally to improve systemic circulation and biological energy production. Based on these, this study investigates whether GSS improved ovarian dysfunction caused by stress in mice. Mice were subjected to stress by electric shock on the foot for 30 min daily for a week and treated with GSS at 500 / body weight per day for one week. Thereafter, changes body weight, adrenal weight, ovulation rate, in vitro and in vivo fertilization, embryonic development and estradiol concentrations were measured. GSS markedly increased the body weight of mice with stress, but not normal mice. The administration of GSS caused a reduction in adrenal weight in stressed mice. GSS also had significant positive effects on ovulation rate, estradiol production, in vivo and in vitro fertilization rates and embryonic development. These results indicate that GSS can improve the reproductive dysfunctions caused by stress, and these may production biological energy.

Mechanism of B-Cell Receptor-Induced Phosphorylation and Activation of Phospholipase C-γ2

Kim, Yeun Ju,Sekiya, Fujio,Poulin, Benoit,Bae, Yun Soo,Rhee, Sue Goo American Society for Microbiology 2004 Molecular and cellular biology Vol.24 No.22

<B>ABSTRACT</B><P>Phospholipase C-γ2 (PLC-γ2) plays an important role in B-cell signaling. Phosphorylation of various tyrosine residues of PLC-γ2 has been implicated in regulation of its lipase activity. With the use of antibodies specific for each of the putative phosphorylation sites, we have now shown that PLC-γ2 is phosphorylated on Y753, Y759, and Y1217 in response to engagement of the B-cell receptor in Ramos cells, as well as in murine splenic B cells. In cells stimulated maximally via this receptor, the extent of phosphorylation of Y1217 was three times that of Y753 or of Y759. Stimulation of Jurkat T cells or platelets via their immunoreceptors also elicited phosphorylation of Y753 and Y759 but not that of Y1217. A basal level of phosphorylation of Y753 was apparent in unstimulated lymphocytes. The extent of phosphorylation of Y753 and Y759, but not that of Y1217, correlated with the lipase activity of PLC-γ2. Examination of the effects of various pharmacological inhibitors and of RNA interference in Ramos cells suggested that Btk is largely, but not completely, responsible for phosphorylation of Y753 and Y759, whereas phosphorylation of Y1217 is independent of Btk. Finally, phosphorylation of Y1217 and that of Y753 and Y759 occurred on different PLC-γ2 molecules.</P>

식품중 치자황색소의 분석법에 관한 연구

김희연,김소희,홍기형,김미순,김현종,이주연,이영자,하상철,이철원,김길생 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

Antibody responses after vaccination against equine influenza in the Republic of Korea in 2013

KIM, Eun-Ju,KIM, Bo-Hye,YANG, Sunjoo,CHOI, Eun-Jin,SHIN, Ye-Jin,SONG, Jae-Young,SHIN, Yeun-Kyung The Japanese Society of Veterinary Science 2015 The Journal of veterinary medical science Vol.77 No.11

<P>In this study, antibody responses after equine influenza vaccination were investigated among 1,098 horses in Korea using the hemagglutination inhibition (HI) assay. The equine influenza viruses, A/equine/South Africa/4/03 (H3N8) and A/equine/Wildeshausen/1/08 (H3N8), were used as antigens in the HI assay. The mean seropositive rates were 91.7% (geometric mean antibody levels (GMT), 56.8) and 93.6% (GMT, 105.2) for A/equine/South Africa/4/03 and A/equine/Wildeshausen/1/08, respectively. Yearlings and two-year-olds in training exhibited lower positive rates (68.1% (GMT, 14) and 61.7% (GMT, 11.9), respectively, with different antigens) than average. Horses two years old or younger may require more attention in vaccination against equine influenza according to the vaccination regime, because they could be a target of the equine influenza virus.</P>