흰쥐에서 심정지후 Pentoxifylline이 뇌의 허혈-재관류 손상에 미치는 효과

정시경,김영민,오동렬,최경호,박승현,이운정,박규남,유은영,이원재,김세경 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Two major events occurring in the cerebral hemodynamics after successful resuscitation from cardiac arrest are reactive hyperemia and postischemic hypoperfusion. In this study, we examined the effect of Pentoxifylline(PTX) on the rat brain following cardiac arrest. Methods: Fourteen rats were anesthetized and artificially ventilated. Cardiac arrest was produced by chest compression and clamping of tracheal tube for 3 minutes in ketamine anesthetized rats. Circulation was restored by standard cardiopulmonary resuscitation methods. In 7 rats, PTX 10mg/kg was infused at 10min after cardiac arrest(PTX group). In the other 7 rats, same amount of normal saline was infused(control group). Results: In both groups, hemodynamic variables, neurologic deficit(ND) score and histopathologic findings of hippocampal CA1 neurons were observed. Hemodynamic variables and ND score were not significantly different between two groups. Delayed ischemic neurons of hippocampal CA1 were decreased in PTX group(2.2±2.4%) compared with control group(9.1±1.2%). Conclusions: We conclude that PTX prevented development of delayed ischemic neurons in hippocampal CA1 after cardiac arrest. PTX may be useful in emergency situations following cardiac arrest.

모유 수유아와 조제 분유 영양아에서 수유 방법에 따른 아미노산 농도 비교 및 혈청 트립토판 농도와 수면 유도와의 관계

정다운,김은영,양은석,박상기,박영봉,문경래 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objective: Sleep behavior is modulated by serotonergic neurons within the brain, and the synthesis and release of serotonin by such neurons is thought to be influenced by the availability of tryptophan, the amino acid precursor of serotonin. Formula-feeding infants have depressed plasma tryptophan concentration relative to breast milk-feeding infants. Because tryptophan alters sleep waking patterns in adults, a study was designed to determine the difference of sleep latency relative to differences in plasma tryptophan and tryptophan : large neutral amino acid (LNAA) ratio between formula-feeding infants and breast milk-feeding infants. Method: 45 newborns who were born in Chosun University Hospital from December 2002 to December 2003 were selected. The newborns were divided to three group, 15 newborns were fed breast milk and 15 newborns were fed formula A, and last 15 newborns were fed formula B. At 6 weeks and 12 weeks, infants were sampled for measuring of serum amino acid level and tryptophan, tryptophan : LNAA ratio. And we taught infants' parents to measure sleep latency that means the time after a feeding to the first episode of active REM sleep that persist ≥ 1 min. Result: 1) At 6weeks, Serum α-amino-n-butyric acid, citrulline, tryptophan level was higher in formula A group than breastmilk group (P<0.05) and proline level was lower than breast milk group (P<0.05). In formula B group, serum n-amino-n-buryric acid, citrulline, cystine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, threonine, valine levels were higher than breastmilk group (P<0.05). And Serum isoleucine, methionine, proline, valine level were higher in formula B group than formula A group (P<0.05). 2) At 12 weeks, serum glutamic acid, methionine, ornithine levels were higher in formula group A than breast milk group (P<0.05). Serum β-alanine, aspartic acid, α-amino-n-butyric acid, ethanolamine, glutamic acid, threonine level were higher in formula B group than breast milk group (P<0.05). Serum β-alanine, ethanolamine levels were higher and arginine level was lower in formula B group than formula A (P<0.05). 3) At 6 weeks, serum tryptophan concentration and tryptophan: LNAA ratio were higher in formula A group (P<0.05). Sleep latency was 21 minutes in formula group A, 24 minutes in breast milk group, 25 minutes in formula B group but there was no statistically significance (P>0.05). 4) At 12 weeks, serum tryptophan concentration and tryptophan: LNAA ratio, time after feeding to the first episode of active REM sleep were no difference with each other group. Conclusion: There is significant difference of serum amino acids between breast-feeding infants and formula-feeding infants. And serum tryptophan and tryptophan: LNAA ratio differ between brest-feeding infants and formula-feeding infants. But there is no association between serum tryptophan, tryptophan: LNM ratio and sleep latency.

취학전 어린이의 운동활동량에 대한 심리만족도 분석

백경운,정병철,신성득 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.1

The purpose of this study was to clarify an psychological satisfaction of exercise for young childrens. The subjects 5-6years(n=210) engaged in touchball for 20 minutes. During that period, both amount of their exercise and joy dervier from the physical activities were measured. The amount of exercise was measured by pedometers As the results, it was deteced that during the 20minutes, the amount of exercise raged approximately from 1,200 to 1,800 in terms of pedometer count, and the degree of active group' s(A group)joy at that time was more than passvise group(B group). The results of this study suggest that the pedometer might be a valid indicator of the daily physical activity. The findings are discussed in terms of the practical applications of the pedometer in the research of daily physical activity.

농인의 관점에서 본 농교육의 전환 : The Deaf Centered Approach

곽정란,정운기,김경진 국립특수교육원 2004 특수교육연구 Vol.11 No.2

본 연구의 목적은 농아동을 특수교육의 대상으로 국한시키는 것에서 벗어나 농교육의 중요한 주체의 하나로서 인식하여, 그들의 요구(needs)를 이해하고 지원하기 위해 농인의 관점에서 농교육의 전환 방향을 모색해 보는데 있다. 이를 위해 첫째, 농인에 대한 사회·문화적 관점은 어떤 내용을 내포하고 있으며, 농인 스스로는 자신을 어떻게 바라보고 있는가, 둘째, 농인의 관점을 지원하기 위한 사회제도는 어떻게 변화하고 있으며, 최근 농사회의 자기권리 주장은 무엇인가, 셋째 농인의 관점에서 농교육의 전환 방향은 무엇인가를 살펴보았다. 고찰 한 결과 농(deaf)의 개념은 청각장애만을 의미하는 것이 아니라 사회·문화적 환경에 따라 다른 의미로 구성될 수 있는 것으로, 이는 농인의 언어인 수화에 대한 사회적 수용에 따라 달라지는 것을 알 수 있었다. 즉 농인에 대한 사회·문화적 관점에 기반할 때 농인은 청각장애인을 의미하는 것이 아니라 수화라는 언어를 사용하는 언어적·문화적 소수집단을 의미하였다. 이에 따라 오늘날의 사회는 수화통역제도 수화통역센타를 통해 농인의 언어적·문화적 입장을 존중하기 위해 변화하고 있으며, 농인 역시 언어적·문화적 권리를 강조하고 있는 것으로 나타났다. 농인의 관점에 입각한 농교육의 전환 방향은 첫째 농아동의 언어적·문화적 배경을 이해하고 지원해 줄 수 있는 농인 교사가 필요하며, 둘째 농인으로서의 정체성을 고려하기 위해 교육과정의 재구성이 요구될 뿐 만 아니라, 셋째 농학교는 본연의 교육적 역할을 충실히 수행함과 아울러 농인의 언어와 문화를 지지하는 문화적·언어적 공간으로서의 역할을 재정립할 필요가 있는 것으로 나타났다. The purpose of this study was find ways (1) to accept people with deaf as a main or core for the education for the deaf, themselves, being over the invisible limitation that they were positioned as subjects - being educated - only on the field, and (2) to reform education for the deaf, according to the stand of view of Deaf people, which was reflected the real needs of Deaf people should be understood and supported. To establish these, the writer investigated following issues; (1) the contents of social and cultural environment of Deaf people, (2) the transition of social approach or system to support needs of Deaf people, which were issued currently on their social and cultural environment and for the more, (3) reformation of education for the deaf that was reflect opinions from or by the deaf. The concept 'deaf' doesn't mean only hearing impairments but being positioned another additional status for the cultural and social environment. The obvious factors to produce the causes of the differences are whether social acceptance and recognition their sign language as their daily communication methods, as people use speaking language. Which means considering their social and cultural environment, we can say people with deaf rather minority who are using sign language in social than handicapped people with hearing impairments. Thus, sign language translating serves, for instance, are providing to support socal and cultural stand of the deaf and ensure appropriate communication between people who are not use sign language and use it. And people with deaf put stress on the right of using their own (sign) language nowadays. The findings of reform education ways reflected view of the culture of the deaf are as follows; First, teachers who are deaf by hisself or herself in be hired in every school for the deaf to fully-understand and support students' social and cultural background, Second, curriculum of deaf education should be restructured to adapt and increase identification as a deaf, Third, schools for the deaf perform to educate deaf more substantially and should be the resource center of sign language and deaf culture.

2상2중쵸퍼를 이용한 축전지 충전제어

이정준,함년근,조정민,김동운,한경희 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

Recently, increasing of portable, importance of battery has identified. And battery is used for non-utility generation and emergency power for communication power, it has researched for power of electric vehicle. But it is disadvantage that life cycle of battery is decreased by overcharging and overdischarging. In this paper, using two phase with combined step-down chopper, we controlled charging and discharging of battery. It is dereased ripple of current and charging time with continuous current by using two phase with combined step-down chopper. Detecting the full charging voltage and cut-off voltage, it is prohibit overcharging and overdischarging with microprocessor.

도시지역에서의 적정택시대수 산정

김경환,순정운 慶尙大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.13 No.-

From ride share aspect, Korean taxis are functioning as public transit. However, taxi-operators are generating social problems on issuing taxi licenses every year, because there is no reasonable method for estimating adequate number of taxis in a city. Thus, a method for estimating adequate number of taxis was developed in this study, the method consists of three criteria; one is the criterion based on optimal taxis' occupancy rate considering optimal waiting time of passengers, the optimal income of operators, and working hours of taxi divers, another is criterion on taxi-passenger chane, the other is the criterion on traffic conditions. Also, a new observation method of taxis' occupancy rate was suggested. In this study, Chinhae was selected as the study city. Followings are the results of this study. First, by using the new observation method of taxis' occupancy rate based on taxi traffic count reliable occupancy data could be collected with low expenses. Second, the results applying the model suggested in this study to Chinhae showed that preset number of axis is adequate and the results matched well with the real situation of Chinhae.

디지털 카메라의 자체검정을위한 도심측정방법과 타원 피팅 방법의 정확도 분석

박운용,정공운,송연경 東亞大學校 建設技術硏究所 2002 硏究論文集 Vol.26 No.1

Digital high-resolution consumer cameras are widely available, and are increasingly used in close-range photogrammetry. The mechanical construction of the cameras often do not meet photogrammetric requirements, thus they have to be modelled sufficiently. An approach for camera modelling is discussed that introduces image-variant parameters of interior orientation. In addition a correction model based on finite elements is integrated that provides the correction of remaining errors in the sensor space. All functional parameters are estimated simultaneously in a bundle adjustment. Significant improvements of object accuracy have been achieved with respect to standard calibration techniques based on self-calibrating bundle adjustment. Rollei d7 metric 디지털 카메라를 이용하여 촬영조건의 다양한 변화에 따른 위치오차를 분석하며 대상물표면에 설치된 타켓의 3차원위치를 결정하기 위해 도심측정방법과 타원 피팅 방법으로 타켓중심을 구하였다. 각 타켓의 변위를 측정하고 3차원 정확도를 분석한 결과 다음과 같은 결론을 얻었다. 도심측정방법과 타원 피팅 방법을 여러 가지로 조합하여 적용한 결과 평균표준오차는 타원 피팅 방법과 도심측정방법을 동시에 적용되었을 경우 X좌표에서 ±0.478mm, Y좌표에서 ±0.478mm, Z좌표에서 ±0.478 mm로 가장 낮은 오차를 보였다. 도심측정방법은 타켓의 모양에 구애받지 않으나 적절한 임계값의 결정이 중요하며 경중률 을 고려함으로써 이를 보완할 수 있었다. 반면에 타원 피팅 방법은 타켓의 형상이 수학적으로 피팅 할 수 있는 형상이어야 한다는 점은 불편하나 임계값 영향을 받지 않았다. 자체 검정기법에 의한 촬영조건의 다양한 변화에 따른 위치오차를 분석하여 Rollei d7 metric 디지털 카메라의 기하학적 지식 및 수행성을 평가하기 위하여 내부표정요소 및 렌즈 왜곡 등에 관련된 기하학적 검정 매개변수들을 도출해 낼 수 있었으며 높은 정확 도 를 나타냄으로써 근거리 지상사진측량시스템 의 잠재성을 확인할 수 있었다. 자체검정 광속조정은 카메라의 계통적 오차들을 효과적으로 보정해줌으로써 정확도를 크게 향상시킬 수 있었다. 따라서 자체검정 기법을 적용하는 디지럴 카메라에 의한 광학적 근거리 지상사진측량시스템은 산업의 차원적 품질 검사,문화재의 3차원 모델링 및 형상재현 등을 효율적으로 구현하여 대상물의 시각적 분석에 활용할 수 있을 것이다.

두개강내 출혈을 동반한 불응성 특발성 혈소판감소성 자반증의 CYCLOPHOSPHAMIDE 치험 1예

서정철,양태영,최연수,박유환,김완중,허경,정원영,정춘해 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Idiopathic thrombocytopenic purpura(ITP) is an autoimmune bleeding disorder characterized by the development of antibodies to one's own platelets, which are then destroyed by phagocytosis in the spleen and liver. Its clinical manifestations are characterized by bleeding and the most serious complication is hemorrhage into the central nervous system, but which is rarely seen below 1%. In treating IPT, the therapy of first chice is administration of adrenocorticosteroid and r-globuline. If satisfactory results are not obtained, then splenectomy is recommended. If this also proves inadequate, then administration of immunosuppressants is carried out. 16-year-old male was admitted with complaints of left hemiplegia and nasal bleeding. This case may be developed spontaneous intracerebral hemorrhage causing thrombocytopenia and was treated by steroid, r-globuline and splenectomy, but satisfactory results are not obtained. After all, the paient was successfully treated with cyclophosphamide.

성인에서 발생한 원발성 종격동 T세포 림프아구성 림프종 : 증례보고

이운하,정미희,정경순 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

림프아구성 림프종은 고등급 악성종양으로서 적절한 치료를 하지 않을시 빠르게 치명적인 경과로 진행한다. 원발성 종격동 림프종은 대부분 호지킨 림프종, 큰 B 세포 림프종(large B cell lymphoma), 림프모구 림프종(lymphoblastic lymphoma)이다. 원발성 T 세포 림프아구성 림프종은 성인에서 드문 형태의 종격동 림프종이며 영상학적 보고는 드물다. 이에 저자들은 성인에서 원발성으로 종격동에 발생한 T세포 기원의 림프아구성 림프종 1예를 경험하였기에 보고하고자 한다. Lymphoblastic lymphoma is high-grade malignancy and rapidly fatal course without appropriate treatment. It is well known as a highly malignant tumor in children, but is rare in adults. Radiologic report of lymphoblastic lymphoma is rare. We report here on a case of a primary mediastinal precursor T-cell lymphoblastic lymphoma in a 46-year-old woman.

Escherichia coli(M15)을 이용한 serotonin N-acetyltransferase 유전자의 발현

김용만,정미영,윤경식,진병관,백행운,조용호,백형환 慶熙大學校 1997 論文集 Vol.26 No.-

AA-NAT cDNA was obtained by RT-PCR technique from total RNA of rat sacrified at night(02:00am). pCRNAT was cloned using the pCRII vector with insertion of AA-NAT cDNA(about 1.4 kb) at EcoRI site. For the expression of the gene, pQECNAT was subcloned, in which the coding region of AA-NAT was inserted into expression vector pQE30 at BamHI and HindIII sites. According to the experimental results, Escherichia coli strain M15, transformed by the expession vector pQECNAT, was selected as a host to express AA-NAT gene with the induction of isopropyl β-D-thiogalactoside(IPTG). Optimal condition for the expression of AA-NAT gene was achieve from the experimental results, showing that the expression of the protein was inducible much 19.800 ± 2,200 dpm per ml of culture volume in 4 hours at the concentration of 2 mM IPTG. Partial purification through the affinity column(Ni-NTA agarose) binding to the continuous 6 histidine residues of protein resulted in 5 times more increase in the specific activity of AA-NAT than that of the homogenate of bacterial pellet. These experimental results will provide basic data in further study for the enzymatic kinetics and antibody production of AA-NAT.