간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

Facileconstruction of Au/SnS nanocomposite for efficient piezocatalysis of aqueous Cr(VI)

( Sun Hao ),박수진 한국공업화학회 2022 한국공업화학회 연구논문 초록집 Vol.2022 No.-

Bimetallic CuPd nanoparticle-decorated MgAl-LDH/g-C3N4 composites for efficient photocatalytic reduction of aqueous Cr(VI)

Hao Sun,Soo-Jin Park 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

Constructing a high-performance photocatalyst is one of the most effective approaches to remove heavymetals from wastewater. Here, we successfully synthesized MgAl-layered double hydroxide/graphiticcarbon nitride/ (CuPd/LDH/CN) decorated with CuPd bimetallic nanoparticles for the first time to photocatalyticallyreduce hexavalent chromium (Cr(VI)) in an aqueous solution. The ternary CuPd/LDH/CNheterojunction exhibited superior photocatalytic reduction of Cr(VI) under visible light compared tothose of pure CN, LDH, and the binary composites. The CuPd bimetallic nanoparticles were deposited ontothe LDH/CN surfaces, as confirmed by morphological observations. Our results revealed that uniquestructural features endow CuPd/LDH/CN catalysts with excellent photocatalytic performance, likelyattributed to abundant active sites, large specific surface areas, as well as enhanced separation and transferefficiencies of photo-generated charge carriers. Particularly, the nanocomposite (with LDH and CuPdcontents of 15 wt% and 1 wt%) exhibited the highest catalytic efficiency with 98% removal of aqueous Cr(VI) (40 mg/L) within 40 min. Furthermore, no significant change in the degradation activity of the CuPd/LDH/CN nanocomposites was observed through four successive experimental runs. This study providesnew insights into the design and construction of high-performance photocatalysts with potential applicationsin environmental remediation and energy conversion.

Diverse fate of ubiquitin chain moieties: The proximal is degraded with the target, and the distal protects the proximal from removal and recycles

Sun, Hao,Mali, Sachitanand M.,Singh, Sumeet K.,Meledin, Roman,Brik, Ashraf,Kwon, Yong Tae,Kravtsova-Ivantsiv, Yelena,Bercovich, Beatrice,Ciechanover, Aaron National Academy of Sciences 2019 Proceedings of the National Academy of Sciences Vol.116 No.16

<P><B>Significance</B></P><P>The canonical targeting signal for degrading proteins by the ubiquitin (Ub) system—a chain composed of multiple Ub moieties—has remained a mystery. The structure of the proteasome, the enzyme that recognizes the signal and degrades the target substrate cannot explain why such a long chain is needed. To better understand this problem, we synthesized α-globin to which chains with different number of Ubs were attached. In long adducts, the proximal Ub remains on the substrate, likely securing its attachment to the proteasome, and is degraded with it. The distal Ub protects the proximal from removal by deubiquitinating enzymes and is then removed and recycled. In short adducts, the Ub moieties are rapidly removed, and the substrate remains stable.</P><P>One of the enigmas in the ubiquitin (Ub) field is the requirement for a poly-Ub chain as a proteasomal targeting signal. The canonical chain appears to be longer than the distance between the two Ub-binding proteasomal receptors. Furthermore, genetic manipulation has shown that one receptor subunit is sufficient, which suggests that a single Ub can serve as a degradation signal. To shed light on this mystery, we chemically synthesized tetra-Ub, di-Ub (K<SUP>48</SUP>-based), and mono-Ub adducts of HA-α-globin, where the distal or proximal Ub moieties were tagged differentially with either Myc or Flag. When incubated in a crude cell extract, the distal Ub moiety in the tetra-Ub adduct was mostly removed by deubiquitinating enzymes (DUBs) and reconjugated to other substrates in the extract. In contrast, the proximal moiety was most likely degraded with the substrate. The efficacy of degradation was proportionate to the chain length; while tetra-Ub globin was an efficient substrate, with mono-Ub globin, we observed rapid removal of the Ub moiety with almost no degradation of the free globin. Taken together, these findings suggest that the proximal moieties are necessary for securing the association of the substrate with the proteasome along the proteolytic process, whereas the distal moieties are important in protecting the proximal moieties from premature deubiquitination. Interestingly, when the same experiment was carried out using purified 26S proteasome, mono- and tetra-Ub globin were similarly degraded, highlighting the roles of the entire repertoire of cellular DUBs in regulating the degradation of proteasomal substrates.</P>

MicroRNA regulation of AMPK in nonalcoholic fatty liver disease

Sun Hao,Kemper Jongsook Kim 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Obesity-associated nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease and is the leading cause of liver failure and death. The function of AMP-activated protein kinase (AMPK), a master energy sensor, is aberrantly reduced in NAFLD, but the underlying mechanisms are not fully understood. Increasing evidence indicates that aberrantly expressed microRNAs (miRs) are associated with impaired AMPK function in obesity and NAFLD. In this review, we discuss the emerging evidence that miRs have a role in reducing AMPK activity in NAFLD and nonalcoholic steatohepatitis (NASH), a severe form of NAFLD. We also discuss the underlying mechanisms of the aberrant expression of miRs that can negatively impact AMPK, as well as the therapeutic potential of targeting the miR-AMPK pathway for NAFLD/NASH.

Deformation of Locally Free Sheaves and Hitchin Pairs over Nodal curve

Hao Sun 대한수학회 2020 대한수학회지 Vol.57 No.4

In this article, we study the deformation theory of locally free sheaves and Hitchin pairs over a nodal curve. As a special case, the infinitesimal deformation of these objects gives the tangent space of the corresponding moduli spaces, which can be used to calculate the dimension of the corresponding moduli space. The deformation theory of locally free sheaves and Hitchin pairs over a nodal curve can be interpreted as the deformation theory of generalized parabolic bundles and generalized parabolic Hitchin pairs over the normalization of the nodal curve, respectively. This interpretation is given by the correspondence between locally free sheaves over a nodal curve and generalized parabolic bundles over its normalization.

Comparison of organic acids supplementation on the growth performance, intestinal characteristics and morphology, and cecal microflora in broilers fed corn-soybean meal diet

Sun, Hao Yang,Zhou, Hong Bin,Liu, Yang,Wang, Yue,Zhao, Cheng,Xu, Liang Mei Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.11

Objective: The aim of this study was to compare the effects of three kinds of organic acid (OA) products on the growth performance, intestinal characteristics and morphology, and cecal microflora in broilers fed a corn-soybean meal meal diet. Methods: A total of 420 one-day-old male Cobb 500 broilers with an average initial body weight of 49.11±1.02 g were used in this 42-day experiment. Birds were randomly allotted to one of five treatments (7 replicates with 12 birds per replicate). Treatments consisted of negative control (NC), basal diet; positive control (PC), basal diet+100 mg/kg of Aviramycin; OA1, basal diet+500 mg/kg of OA product 1; OA2, basal diet+1,000 mg/kg of OA product 2; and OA3, basal diet+1,200 mg/kg of OA product 3. Results: The results indicated that OA product addition had no effect on growth performance parameters, such as body weight gain, feed intake, and feed conversion ratio, from days 1 to 14, 15 to 28, and 0 to 42, or on the pH values of the intestine, intestinal weight, or intestinal weight to body weight ratio. The intestinal morphology in terms of villus height and crypt depth were affected by dietary supplementation of OA products, respectively. Furthermore, dietary addition of OAs had positive influences on the maintenance of the cecal microflora based on the results of 16S rRNA analysis. Conclusion: Dietary inclusion of three kinds of OA products all benefit broilers, but the mode of action may be different. This study provides a basis for the application of OA products used in the poultry industry.

Self-nitrogen-doped carbon materials derived from the petioles and blades of apricot leaves as metal-free catalysts for selective oxidation of aromatic alkanes

Sun Yongbin,Hao Junlei,Zhu Xuesai,Zhang Baobin,Yin Hao,Xu Shanguang,Hou Chao,Liu Kun 한국탄소학회 2020 Carbon Letters Vol.30 No.2

Carbon materials with tailorable structures and superior properties have great potential applications in environmental protec�tion, energy conversion, and catalysis. Plant biomass as abundant and green non-toxic raw materials has been considered as good precursors for synthesizing heteroatom-doped carbon materials. However, few studies have been reported on the dif�ferent natures of carbon materials derived from diferent parts of the same plant biomass. In this study, we prepared carbon materials from the petioles and blades of apricot leaves by direct pyrolysis without additives. Detailed characterizations indicate that these two carbon materials are similar in element composition and graphitization degree, but difer greatly in surface area and pore volume. These diferences can be attributed to the diferent contents of inorganic salts, vascular bundles, and proteins in petioles and blades. When used as catalysts for the oxidation of ethylbenzene, the petiole-derived carbon shows better catalytic performance than the blades derived carbon due to its high surface area, large average pore size, and doped nitrogen atoms. Furthermore, the carbon catalysts derived from the petioles and blades of poplar leaves and parasol tree leaves show the same diference in catalytic reaction, implying that the above-mentioned conclusion is rather universal, which can provide reference for the synthesis of carbon materials from leaves.

A sequential fuzzy diagnosis method for rotating machinery using ant colony optimization and possibility theory

Hao Sun,Ke Li,Huaqing Wang,Xueliang Ping,Yi Cao 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.4

This study proposes a novel intelligent fault diagnosis method for rotating machinery using ant colony optimization (ACO) and possibilitytheory. The non-dimensional symptom parameters (NSPs) in the frequency domain are defined to reflect the features of the vibrationsignals measured in each state. A sensitive evaluation method for selecting good symptom parameters using principal componentanalysis (PCA) is proposed for detecting and distinguishing faults in rotating machinery. By using ACO clustering algorithm, the synthesizingsymptom parameters (SSP) for condition diagnosis are obtained. A fuzzy diagnosis method using sequential inference and possibilitytheory is also proposed, by which the conditions of the machinery can be identified sequentially. Lastly, the proposed method iscompared with a conventional neural networks (NN) method. Practical examples of diagnosis for a V-belt driving equipment used in acentrifugal fan are provided to verify the effectiveness of the proposed method. The results verify that the faults that often occur in V-beltdriving equipment, such as a pulley defect state, a belt defect state and a belt looseness state, are effectively identified by the proposedmethod, while these faults are difficult to detect using conventional NN.

Active mass damper control for cable stayed bridge under construction: an experimental study

Hao Chen,Zhi Sun,Limin Sun 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.38 No.2

A cable stayed bridge under construction has low structural damping and is not as stable as the completed bridge. Control countermeasures, such as the installation of energy dissipating devices, are thus required. In this study, the general procedure and key issues on adopting an active control device, the active mass damper (AMD), for vibration control of cable stayed bridges under construction were studied. Taking a typical cable stayed bridge as the prototype structure; a lab-scale test structure was designed and fabricated firstly. A baseline FEM model was then setup and updated according to the modal parameters measured from vibration test on the structure. A numerical study to simulate the bridge-AMD control system was conducted and an efficient LQG-based controller was designed. Based on that, an experimental implementation of AMD control of the transverse vibration of the bridge model was performed. The results from numerical simulation and experimental study verified that the AMD-based active control was feasible and efficient for reducing dynamic responses of a complex structural system. Moreover, the discussion made in this study clarified some critical problems which should be addressed for the practical implementation of AMD control on real cable-stayed bridges.