Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

심한 척추변형(측만증 및 후만증)에 대한 척추 후방 절제술

석세일,김진혁,이상민,정의룡,이정희,김성수,정락용 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1S

■ Purpose To report a technique of vertebral column resection through a single posterior approach and its first results in the treatment of severe spinal deformities. ■ Methods and materials Seventy spinal deformity patients treated by posterior vertebral column resection(PVCR) were reviewed. Minimum follow up was 2 years (2 - 3.3 years). There were 34 males and 36 females with a mean age of 27.4 years at the time of the operation. Etiologic diagnoses were adult scoliosis in 7, congenital kyphoscoliosis in 38, and post-infectious kyphosis in 25. The surgery consisted of temporary stabilization of the vertebral column with segmental pedicle screw fixation, resection of the vertebral column at the apex of the deformity via the posterior route followed by gradual deformity correction and global fusion. ■ Results The total number of resected vertebrae were 143; 76 in thoracic and 67 in lumbar. Mean operation time was 4 hours 31 minutes with average blood loss of 2333 ml. The deformity correction was 40o in the coronal plane and 5Oo in the sagiotal plane. Complications were encountered in 24 patients: 2 complete cord injuries in severe adult scoliosis and thoracic kyphosis patient who had significant preoperative cord compromise, 6 hematomas, 4 root injuries (all incomplete), 5 fixation failures, 2 infections and 5 hemopneumothoraxes. ■ Conclusions PVCR is an effective alternative for severe fixed spinal deformities. However, extreme caution must be taken in the patients with preoperative cord compromise who are the high risk of neurological complications.

역행성 골수강내 금속정을 이용한 대퇴골 과상-과간 골절의 치료

정필현,황정수,강 석,김종필,전용진 대한골절학회 2004 대한골절학회지 Vol.17 No.2

분절간 척추경 나사 고정과 척추전이법을 이용한 특발성 척추 측만증의 치료

석세일,김진혁,이상민,정의룡,김성수,이정희,박준석 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Objective : To report the clinical result of segmental pedicle screw fixation and translation technique in surgical correction of idiopathic scoliosis. Methods and materials : Ten idiopathic scoliosis. patients treated with segmental pedicle screw fixation and translation technique were analyzed retrospectively. The mean follow-up interval was 23.8months, with a minimum of 12 months. Eighteen structural curves were fused in one double curves(King type Ⅰ), seven double thoracic curve(King type Ⅴ) and two thoracolumbar curve. Deformity correction, apical vertebral rotation(AVR), apical vertebral translation(AVT), trunk balance, shoulder balance and complications were evaluated. Result : The structural curve was corrected from 47.4±10.6° to 20.3±5.9° at 1week after surgery and 55.6±14.1° at latest follow-up. AVR was corrected from 2.06±0.4 to 0.94±0.41 at 1week after surgery and latest follow-up. AVT was improved from 25±15.7㎜ to 11.5±7.3㎜ at 1week after surgery and 12.3±7.3㎜ at latest follow-up. There were no trunk imbalance(less than 10㎜ distance from C7 plumb line), no shoulder imbalance(less than 10㎜ shoulder height difference) and no screw related complications. Conclusions : The segmental pedicle screw fixation and translation technique is an effective method in treating for idiopathic scoliosis.

드라마틱 발레의 형식적 특성에 관한 연구

정종훈,이진숙 東亞大學校附設스포츠科學硏究所 2004 스포츠科學硏究論文集 Vol.22 No.-

The ballet, as a representative genre of theatrical art, has developed with constant opposition between dramatic ballet laying emphasis upon its dramatic characteristics and plotless ballet putting stress on integrity of dance. In this respect, this study attempts to investigate some types of ballet with its brief historical survey. Jean George Noverre(1727-1810), a historical father of dramatic ballet, insisted union of dancing and drama on the basis of his theory "ballet diction". Mikhail Fokin(1880-1942) in the early 20th century, experiments a possibility of "new ballet" with a stress upon synthesis of ballet, completing its "five principles" and he values human's inner emotion and its expression above everything else. In the mid 20th century, Antony Tudor(1908-1987), a successor of M. Fokine's spirit, raised up the level of formal characteristics of dramatic ballet, creating a genre called "psychological ballet". In addition, Kurt Joose(1901-1979) and John Granko(1927-1973) created a structural characteristic of dramatic ballet. Its main contents are as follows : First, plot, a composition of story required to dramatic ballet, is an essential requirement providing the inner order of work. Second, persons or characters are the main element developing story, Third, action is only direct means to understand persons, and prior to others. Fourth, uniting all expressive elements of theatrical art make sure the synthetic feature which dramatic ballet is pursing as a total art. In conclusion, it can be expected in this study that the formal features of dramatic ballet and its types according to the times would be recognized and received more seriusly and deeply in understanding and producing ballet.

유리 전박골피판을 이용한 음경 재건술

정성광,변진석,백봉수,김법완 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Reconstruction of the penis is indicated in a traumatic or surgical amputation, congenital penile absence, micropenis, male pseudohermaproditism, or transsexualism. Initially, penile reconstruction has been performed using a local flap, but often failed because of the multiple procedures, fistula or stricture of the urethra, and no sensation of the phallus. Ideally, penile reconstruction should be a one-stage procedure, creating a phallus with both tactile and aerogenous sensibility, water-tight neourethra allowing for voiding while standing, enough bulk with stiffness, and aesthetically acceptable appearance. Since August 1989 we have constructed a penis in 4 patients. We selected the radial forearm flap as a donor site because it offers a reliable artery, veins, nerves, and stiffner. It also offers thin, malleable skin with adequate width and length. We have followed these patients from 5 months to 10 months. All operations were successful except for one fistula formation.

후방 도달 척추 절제술을 이용한 강직성 요천추 변형의 치료

석세일,정의룡,이정희,김진혁,김성수,이지호,최원기,박용원 대한척추외과학회 2004 대한척추외과학회지 Vol.11 No.2

한국인 만성 간 질환 환자에서 G형 간염 바이러스 감염의 유병률에 대한 연구

이진호,이한주,윤정환,이효석,김정룡 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.31 No.3

흰쥐의 방사선 피폭후 생물학적 지표로서의 혈액효소

김진규,김상복,김국찬,천기정,김인규,박효국,이강석 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

생물학적 선량평가를 위한 생화학적 지표 연구로서 흰쥐 혈액내 효소활성도의 변화를 조사한 결과는 다음과 같다. 1) Alkaline phosphatase 활성도는 0.1, 0.25, 0.5, 2, 4 Gy의 방사선 조사후 24시간까지 혈액내 활성도가 증가하였고 72시간 경과시에는 대조군과 비슷한 활성도를 보였다. Creatine kinase는 2, 4 Gy 방사선 조사후 혈액내에서 활성도에서 활성도가 72시간까지 증가하였으나 0.1, 0.25Gy, 0.5 Gy 방사선 조사시에는 커다란 변화를 보이지 않았다. 2) Malate dehydrogenase 활성도는 0.1, 0.25, 0.5 Gy 방사선 조사시에는 커다란 변화가 없었으며 lactate dehydrogenase는 방사선 조사후 활성도가 감소하였다. 3) GOT의 활성도는 선량률 0.1Gy/min.로 0.1, 0.25, 0.5, 2, 4 Gy 조사후에는 어떠한 변화도 없었으며 선량률 0.5 Gy/sec.로 0.5, 1, 1.5, 2, 3, 5, 7 Gy로 조사후에는 증가현상을 보이고 있다. Acid phosphatase 활성도는 상기의 어떠한 선량에서도 나타나지 않고 있다. 잠재적으로 이러한 효소들은 방사선 피폭의 지표물질로 사용될 수 있으며 생화학적 지식과 기술을 이용한 좀더 나은 지표물질을 찾기 위하여 계속적인 조사가 필요하다. Enzyme activity changes in rat blood as biochemical indicator useful for evaluating exposure dose were experimentally studied. The experimental results obtained are as follows: 1) Alkaline phosphatase activities increased in the blood serum until 24 hours after 0.1, 0.25, 0.5, 2 and 4 Gy irradiation and its activities returned mormal condition after 72 hours of post-irradiation. Creatine kinase activities increased in the blood serum until 72 hours after 2 and 4 Gy irradiation but any significant activity changes were not detected after 0.1, 0.25 Gy irradiation. 2) Malate dehydrogenase activities did not reveal available changes changes after 0.1, 0.25, 0.5 Gy irradiation and lactate dehydrogenase activities decreased in the blood serum after 0.1, 0.25, 0.5 Gy irradiation. 3) Glutamate oxaloacetate transaminase activity changes were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and GOT activities increased after 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.). Any acid phosphatase activities were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.) irradiation. Potentially some of these enzymes can be used as indicator protein for radiation injury. Futher investigation is needed to find better biochemical indicatiors utilizing recent knowledge and techniques of biochemistry.

전슬관절 동통환자의 등속성 근력 평가에 관한 연구

柳碩柱,李聖喆,朴泰永,柳文集,金明昊,鄭宣根 단국대학교 1995 論文集 Vol.29 No.-

We studied 25 cases of patients with anterior knee joint pain without any other knee problems with a isokinetic evaluation technique using Cybex. The results are 1. The peak torques for the knee extensor muscles were decreased statistically 2. The peak torques for the knee flexor muscles were decreased relatively also but not statistically 3. TAE(Torque Acceleration Energy) values for knee extensor & flexors were all decreased from disuse atrophy 4. The torque curve for extensors showed decreased power around 30 degrees of knee flexion