분만혈액을 이용한 HLA Class I 검사용 tray의 제작을 위한 연구

권소영,강성은,박경신,황유성,김상인 大韓輸血學會 1999 大韓輸血學會誌 Vol.10 No.1

비행청소년과 일반청소년의 자아존중감과 의사소통유형의 비교연구

소용주,강문희 서울여자대학교 특수치료전문대학원 2007 심리치료: 다학제적 접근 Vol.7 No.1

본 연구의 목적은 비행청소년과 일반청소년의 자아존중감과 의사소통 유형의 차이를 비교하는 것이다. 비행청소년과 일반청소년의 자아존중감과 의사소통유형을 비교했다. 이를 위해 자아존중감 검사와 자녀가 지각하는 의사소통 유형 검사지를 사용했다 연구대상은 흡연,음주,도벽,폭력 등의 일탈 행동을 하는 서울과 수도권의 중학생,학교에서 청소년 상담실에 의뢰되거나 학교 상담실로 의뢰된 청소년들을 비행청소년으로 규정하고 100명을 조사했다. 그리고 같은 연령대의 일반청소년 100명을 대상으로 두 집단 간의 자아존중감과 의사소통 유형의 차이에 대한 조사를 실시하였다 그 결과 비행청소년이 일반청소년에 비해 자아존중감이 낮고,문제형 의사소통을 더 많이 하는 것으로 검증되었다. Purpose of this study is comparing self-esteem and communication type between delinquent juvenile and general juvenile. This study compared self-esteem and communication type between delinquent juvenile and general juvenile. For this study,a self-esteem test and a testing form of communication type which children can perceive are used. To examine,the object of study are one hundred delinquent juveniles living in Seoul and the Metropolitan area who were also asked to a juvenile counselor office or a school guidance office from school because of their maladjustment behaviors such as smoking,drinking,gambling,and using violence. Also this study examined one hundred general juveniles of the same ages to compare differences of self-esteem and communication type between two groups. The results of study verify delinquent juveniles have lower self-esteem than general juveniles,and use more controversial communications.

씨름선수에서 체중 감량과 회복기의 Leptin과 PAI-1 변화

김용운,도경오,권태동,박덕일,장응찬,박소영,김종연,이석강 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Obesity which is defined as accumulation of excess body fat, is central factor of insulin resistance syndrome. Recently, it is revealed tat adipose tissue is not simply an energy storage organ but it also secretes a variety of molecules which affect the metabolism of the whole body, those are leptin and plasminogen activator inhibitor-1(PAI-1). Therefore, leptin and PAI-1 are increased in the obese state. Leptin regulates energy homeostasis and satiety and PAI-1 regulates fibrinolytic system. For these reasons, elevated levels of leptin and PAI-1 are considered as link factors between obesity and insulin resistance syndrome. However, the exact regulating mechanism for serum levels of leptin and PAI-1 is not fully understood yet. In this study, to evaluate the regulating mechanisms of serum levels of leptin and PAI-1 according to the weight changes, we measured leptin, PAI-1, physical, metabolic, and endocrine parameters during 10 days of weight reduction and 10 days of regain period in 7 young athletes. The mean body weight change was -4.7 kg(5.0%) in the weight reduction period and -2.4 kg(2.5%) in the weight regain period compared to baseline value. Baseline level of leptin in athletes was 1.7±0.66 ng/ml, which was significantly correlated with body weight, BMI, percent body fat, body fat mass, triglyceride, insulin, and PAI-1. Baseline level of PAI-1 in athletes was 16.6±5.26 ng/ml, which was significantly correlated with body weight, BMI, triglyceride, insulin, and leptin. Leptin was decreased to 0.7±0.39(44% of the basaline value) in the weight reduction period, and increased to 1.9±0.64(119% of the baseline value) in the regain period. PAI-1 was decreased to 7.4±2.72(44% of the basaline value) in the weight reduction period, and increased to 22.8±7.33(138% of the baseline value) in the regain period. The changes of leptin during weight reduction period were significantly correlated with the changes of insulin(r=0.890, P<0.01) and triglycerides(r=0.874, P<0.01). The changes of PAI-1 during weight reduction period were significantly correlated with the changes of FFA(r=0.889, P<0.01) and triglycerides(r=0.869, P<0.05). The changes of both leptin and AAI-1 during weight regain period were significantly correlated with the changes of insulin(r=0.755 and 0.849, P<0.05, respectively). In summary, these results suggest that serum levels of leptin and PAI-1 were affected by weight cycling, the percentages of change were more greater than that of weight change, and rebound phenomena were occurred during weight regain period.

서동증과 의지감소증으로 발현한 간성 뇌병증 : 증례보고

문소영,정용,강수진,진주희,나덕렬 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

We report a 70-year-old woman who, without a prior history of liver disease, presented abulia and bradykinesia as the initial symptoms of hepatic encephalopathy (HE). Neurological examination showed bilateral extensor plantar responses in addition to mild parkinsonian features On neuropsychological tests, she was impaired at the attention, visuospatial function, memory, and frontal lobe function. Laboratory tests were remarkable for increased AST and ammonia, and positive igG HbcAb. The results of ultrasonography of the liver were consistent with chronic liver disease. Brain T1 weighted MRI revealed a high signal intensity in bilateral basal ganglia. Brain PET showed diffuse cerebral hypometabolism After treatment with oral lactulose, the above symptoms remained improved for the next 6-month of follow-up From this case, we learned that hepatic encephalopathy could be a reversible cause of dementia plus parkinsonism even in patients without a prior history of chronic liver disease.

한국 재래 산양에서 Leptospira spp.에 대한 혈청역학적 연구

한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

Between January 1999 and September 2001, 948 sera (120 from each province except 108 from Chungbuk) from 8 provinces were sampled from Korean goats. All the samples were serologically tested for Leptospira spp. By the microscopic agglutination test against four L. interrogans serovars including canicola, icterohaemorrhagiae, pomona and hardjo, 948 Korean goats were confirmed as 11 seropositives (1.2%) only infected with L. icterohemorrhagiae. All positive sera were collected from Gyeonggi (5), Gangwon (4) and Chungbuk (2). Titration of them were classified as 1:100 of 10 sera except one case with 1:200 from Gyeonggi. It was meaningful that the pathogen in Korean goats was mainly distributed in the northen part of the country. Consequently, the results in the study were confirmed and analyzed the recent status of infection of leptospirosisas zoonotic agentsome in Korean goats.

한국 재래산양에서 Bovine Viral Diarrhea, Bovine Herpesvirus-1, Parainfluenza Type-3 바이러스에 대한 혈청학적 연구

한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

The 948 goat sera were serologically tested for ten pathogens including bovine viral diarrhea (BVD), bovine herpes virus type-1 (BHV-1) and parainfluenza virus type-3 (PI-3). By viral neutralizaion test, three pathogens including BVD virus, BHV-1, PI-3 were applied for screening each neutralizing antibody and its titration. Among 948 sera, there were 215 seropositives (22.7%) against BVD virus, 25 (2.6%) against BHV-1 virus, and 161 (17.0%) against PI-3 virus, respectively. In the seropositive cases to BVD virus, provincial prevalence of them were recorded as Gyeongnam(30.8%) for the highest one, while as Jeonbuk (15.8%) and Gyeongbuk(l6.7%) relatively for the lower ones. Among them, higher than 1:8 in antibody titration was classified as 74 cases (34.4%) which were suspected the cycle of its infection in their herds. Among the seropositives to BHV-1, it was relatively lower prevalence rate ranging from 1.7% to 6.7% that those of BVD virus and PI-3, but more importantly they were prevailing across the country. Most of the seropositive cases (42.9%) to PI-3 were classified as 1:4-1:16 in titration, but also distributed all around the country. The prevalence in goats varied according to areas (15.8 to 30.8%). Related to the provincial prevalence of three viral agents in Korean goats, it seemed that three viral pathogens would be directly or indirectly involving in various disorders as primary or secondary causative agents resulting in decreased productivity.

채소류에 의한 카드뮴과 납의 흡수에 관한 연구

박병윤,이부용,양소영,강상재,이동훈 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.6 No.1

본 연구에서는 전통적으로 우리 식탁의 주요 부식으로 이용되어온 콩나물과 미나리를 Cd와 Pb가 함유된 배양액으로 재배하는 동안 이들 채소에 Cd와 Pb 가 어느 정도 흡수되는가를 조사하였다. 또한 수경재배에서 Cd와 Pb에 의한 콩나물의 생육장해 정도를 조사하였다. 배양액속에 함유된 Pb이온은 재배기간 동안 미나리와 콩나물 체내로 쉽게 흡 수되었다. 그리고 미나리는 콩나물보다 더 많은 양의 Pb를 흡수하였는데, 이 는 미나리의 줄기에 다량의 뿌리가 붙어있기 때문인 것으로 생각된다. 또한 배양액속의 Pb의 농도와 미나리와 콩나물 체내에 축적된 Pb의 함량과는 높은 상관성을 나타내었는데, 각각의 상관계수(r^2)는 0.9411, 0.9828이었다. Cd역시 Pb와 마찬가지로 재배기간 동안 미나리와 콩나물 체내로 쉽게 흡수되 었다. 또한 배양액속의 Cd의 농도와 미나리와 콩나물 체내에 축적된 Cd의 함 량과는 높은 상관성을 나타내었는데, 각각의 상관계수(r^2)은 0.9900, 0.9054이었다. 그러나 두 식물체에서 Pb의 흡수 경우와는 달리 배양액속에 함유되어 있는 Cd의 농도가 높을 경우 콩나물이 미나리보다 더 많은 Cd을 흡수하였다. 콩나물의 경우에는 Cd과 Pb이 재배기간 동안 거의 비슷한 양으로 흡수되었으며, 그리고 배양액 속에 함유된 이들 중금속들과 콩나물 체내에 축적된 중금속들 사이의 직선회귀선의 기울기도 거의 유사하였다. 그러나 미나리의 경우에는 Pb이 Cd보다 상당히 많은 양이 흡수되었으며, 또한 직선회귀선의 기울기도 Cd보다 Pb의 경우 상당히 크게 나타났다. Cd와 Pb의 농도가 1mg/l일 경우에는 콩나물 길이의 성장 또는 외관상의 생육에 거의 영향이 없었다. 그러나 10mg/l 농도에서부터는 길이의 성장장해뿐만 아니라 외관상으로도 분명한 장해현상이 나타났다. 50mg/l와 100mg/l의 농도에서는 콩나물의 생육장해가 더욱 심해졌다. Pb의 경우에는 100mg/l의 농도에서, Cd의 경우에는 50mg/l의 농도에서부터 재배기간 동안 콩나물의 상당 부분이 썩는 현상이 나타났다. This study was conducted to investigate the uptake of Cd and Pb by vegetables. Bean sprout and dropwort, which are very important for traditional korean dishes, were cultivated in culture fluid added with Cd and Pb cations, and the content of Cd and Pb absorbed by these vegetables were measured. Pb cations in culture fluid were easily absorbed into bean sprout and dropwort. And dropwort absorbed Pb more than bean sprout did. Pb contents of bean sprout and dropwart dramatically increased with increasing Pb concentration in culture fluid. The correlation coefficients(r^2) between Pb contents in dropwort and bean sprout and Pb concentrations in culture fluid were 0.9411 and 0.9828, respectively. Cd cations were also easily absorbed into bean sprout and dropwort. The correlation coefficients(r^2) between Cd contents in dropwort and bean sprout and Cd concentrations in culture fluid were 0.9900 and 0.9054, respectively. The growth of bean sprout was not hindered at the concentration of 1mg/ℓ Cd and Pb in culture fluid. But high Cd and Pb concentrations hindeded remarkably the growth of this vegetable. A large part of bean sprout was rotten at the concentrations of 50 mg/ℓ Cd, and 100 mg/ℓ Pb.

Anti-osteoporotic Effects of Sericin-Calcium Conjugate in Ovariectomized Rats

Ehn-Kyoung Choi, Dongsun Park, So-Young Choi , Yun-Hui Yang, Seock-Yeon Hwang, Seongho Yeon, Jeong-Yong Lee, Ill-Hwa Kim, Yun-Bae Kim, Hyun-Gu Kang1 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.2

This study was conducted in order to evaluate the effect of sericin-calcium (SC) as therapy for ovariectomy-induced osteoporosis in rats. Three weeks after ovariectomy (OVX), Sprague-Dawley rats were divided randomly into three groups: sham-operated group (Sham), ovariectomized group, and SC-treatment group (OVX+SC). Rats in the OVX+SC group were given drinking water containing 0.07% SC for eight weeks. Bone breaking force, mineralization, and blood parameters related to bone metabolism were analyzed. In OVX animals, blood concentration of 17β-estradiol showed a significant decrease, while osteocalcin and type I collagen C-terminal telopeptides (CTx) showed an increase. Breaking force of femurs as well as bone mineral density (BMD), ash, calcium, and phosphorus in femurs showed a significant decrease following OVX. Treatment with SC (0.07% in drinking water) resulted not only in remarkable restoration of the decreased 17β-estradiol and increased osteocalcin and CTx concentrations, but also led to recovery of decreased femoral breaking force, BMD, ash, calcium, and phosphorus. It is suggested that SC effectively improves bone density by preventing bone turnover-mediated osteocalcin, CTx, and minerals, and that it could be a potential candidate for use in therapy or prevention of post-menopausal osteoporosis.

Antifungal activities of zoysiagrass (Zosia japonica Steud.) chitinases against Rhizoctonia solani and analysis of fungus responsive cis-elements in chitinase genes promoter

Ji-Nam Kang,So-Mi Kang,Hong-Gyu Kang,Hyeon-Jin Sun,Yong-Ik Kwon,Suk-Min Ko,Hyo-Yeon Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Zoysiagrass are damaged by fungi diseases such as large patch, dollar spot, pythium blight and brown patch. Large patch is one of the major diseases caused by Rhizoctonia solani AG2-2 on zoysiagrass fields e.g. golf courses. Plant chitinases have been known PR (Pathogen related)-protein. In this study, we isolated two chitinase genes (Zjchi1 and Zjchi2) from zoysiagrass. Antifungal activity analysis revealed that Zjchi2 protein inhibited mycelium extension of fungi. A further study, we cloned 5` upstream region from two chitinase genes for investigating transcription regulatory mechanism that inducing of two chitinase genes dependent R. solani. -818 bp and -799 of upstream region from Zjchi1 and Zjchi2 successfully isolated using in vitro LA (Long and Accurate) PCR system. And then, we generated promoter-GUS reporter constructs with deletion construct based on W-boxes. Constructs were introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation for stable expression of GUS reporter gene.

Optimized progerin-Lamin A binding inhibitor, Progerinin, ameliorates premature senescence phenotypes of Hutchinson Gilford progeria Syndrome and Werner Syndrome

( So-mi Kang ),( Min-ho Yoon ),( So-young Park ),( Tae-gyun Woo ),( Ah-young Oh ),( Nam-chul Ha ),( Gyu-yong Song ),( Bum-joon Park ) 한국장기요양학회 2018 한국장기요양학회 추계학술대회자료집 Vol.2018 No.-

In our previous study, we found that progerin-Lamin A binding inhibitor (JH4) can improve the pathological features of Hutchinson Gilford progeria syndrome (HGPS) such as nuclear deformation and growth suppression in patient’s cells and very short life span in in vivo mouse model (1,2). Despite favorable effect of this chemical, JH4 is very rapidly eliminated in in vivo pharmacokinetic (PK) analysis. Thus, we improved the chemical property through chemical modification and finally obtained the optimized drug candidate, Progerinin (Code No. SLC-D011). This chemical can extend 5 weeks of life span of Lamn^G609G/G609G mouse (from 14.5 week to 19.5; maximum life span is 20 week) with increase of body weight. In addition, we can observe the favorable effect of progerinin in werner syndrome (WS) cells, such as enhancing cell proliferation and inhibition of SA-β-galactosidase expression. Moreover, elimination of progerin in WS cells can restore the nuclear morphology and enhance H3K9me3 expression. These results indicate that progerinin is one of strong candidate for HGPS as well as WS syndrome.