複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

E. coli 과량발현을 통한 B형 간염 바이러스 preS1 유도체의 안정한 동위원소 N의 치환

박병관,김세하,한규훈,김선영,홍요정,이시형,한동설,김순종,박진구 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

We have used molecular biology technique to label the side chains and backbone of HBV preS1(1-56) with stable isotope (??) during E. coli over-expression and purified the protein to near homogeneity. The expression level of ?? labelled preS1(1-56) seems to be ∼15% of the total protein. to characterize the secondary structure of the labelled protein, circular dichroism was used. In different buffer conditions including pH 3.3 and pH 7.4 the protein seems to be devoid of any stable secondary structure probably suggesting that the N-terminal portion (preS1(1-56)) of HBV preS1 might be unstructured before binding to its target cells.

관상동맥 환자에서 관상동맥 스텐트 삽입술에 관한 임상경험

박시훈,신길자 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.2

Objective : The placement of stents in coronary arteries has been shown to be effective bail out procedure and reduce restenosis in comparison to balloon angioplasty. We experienced coronary stenting in 8 patients with coronary artery disease and report our results. Method : From October 1995 to April 1996, 8 cases of coronary artery stenting were per-formed at the Ewha Womans University Hospital. We evaluated results of the procedure, in-hospital complications, follow-up coronary angiography and follow-up clinical events. Result : All lesions were successfully stented without in-hospital complications including death, myocardial infarction, repeat coronary angioplasty, or CABG. During 2 months of fol-low-up, there was no clinical events. Follow-up coronary angiography was performed in one case, showing no restenosis. Conclusion : Acute procedural results and angiographic and follow-up clinical outcomes were favorable, so coronary stenting seemed to be good therapeutic tool in treatment of coronary artery disease.

류마티성 승모판 협착증 환자에서 Inoue 풍선을 이용한 경피적 승모판 풍선 성형술의 임상 경험

박시훈,신길자 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.1

Object : Percutaneous mitral balloon valvuloplasty was introduced by Dr. Inoue in 1984 and it is now widely used in the treatment of mitral stenosis due to its simple design to use. Method : From September 1993 to January 1996, 11 cases of percutaneous mitral balloon valvuloplasty were performed at the Ewha Womans University Hospital. Result : Following the percutaneous mitral balloon valvuloplasty, the mitral valve opening area was increased from 1.18±0.34㎠ to 1.91±0.62㎠(p<0.001). The mean mitral valve diastolic pressure gradient was decreased from 14.35±6.00mmHg to 5.41±4.03mmHg.(p<0.001) The NYHA functional class was improved significantly. Among 11 cases, 1 case(9%) was failed to expand mitral valve opening area and another 1 case developed grade 2 of mitral regurgitation. But there was no life threatening complication such as cardiac tamponade. Conclusion : It is concluded that percutaneous mitral balloon valvuloplasty with Inoue alloon is an effective and simple method for most patient with mitral stenosis who do not have thrombi.

대퇴동맥을 이용한 도자술에 합병된 대퇴 가성 동맥류의 비수술적 치료 : 수기 압박과 C-clamp의 병용사용

박사영,박시훈,신길자,이우형 梨花女子大學校 醫科大學 醫科學硏究所 1995 EMJ (Ewha medical journal) Vol.18 No.3

Femoral pseudoaneurysm is important complication after diagnostic femoral catheterizationor more complex procedure. With the increasing use of larger-size percutaneous instruments and periprocedual anticoagulant or antiplatelet agent the incidence of postcatheterization femorl artery injuries ncluding pseudoaneuiysm has increased in the past few years. Duplex ultrasonography and addition of color- flow Doppler provides an accurate, noninvasive. risk-free diagnosis and faster detection of intraaneurysrnal blood flow and the track betweenthe injured artery and the pseudoaneurysm. Though early surgical repair of the arterial defect is usually recommended because of severeand life-threatening complication such as rupture, fhrornboembolism, compression neuropathyetc, Ultrasono-Guided Compression Repair(UGCR) is to be first-line treatment for its advantagesuch as high success rate, low morbidity and cost-effectiveness. The authors report 2 cases of femoral psoudoaneurysrns treated using UGCR with nlanualcompression with C-clamp at the same time as a nonsurgical treatment.

재발성 다발성 연골염

박용범,이시훈,정상윤,이수곤,홍순원,김정민,이창률 대한내과학회 2001 대한내과학회지 Vol.61 No.3

Polymethylmethacrylate Microspheres(PMMA-NewPlastic®)로 치료한 칼자국상 2예

박정훈,오두진,강승훈,황선욱,조시형,박성욱 대한피부과학회 2006 대한피부과학회지 Vol.44 No.4

신체적 긴장후에 유발되고 애성이 동반된 대동맥 박리 1예

이기현,박시훈,신길자,이우형 梨花女子大學校 醫科大學 醫科學硏究所 1995 EMJ (Ewha medical journal) Vol.18 No.4

본 저자들은 고혈압이나 지지조직의 병변, 선천성 심장질환등의 유발 인자 없이 지내던 환자가 갑작스런 신체적긴장으로 흉통이 유발된 후 진행성 애성이 발생하여 애성의 원인 진단 및 치료를 목적으로 내원한 후 대동맥 박리를 진단받은 사례를 경험하였기에 이에 보고하는 바이다 Aortic dissection is caused by a circumferential or transverse tear of the intima by a discrepancy between the strength of the aortic wall and the intraluminal pressure. Arterial hypertension seems clearly to be a factor in the genesis of aortic dissection. An elevated blood pressure or evidence of its existence can be found in 70-80 percent of patients. Other factors predisposing to aortic dissection are congenital disorder of connective tissue, vasculitis, etc. But trauma or physical strain is unusual cause of classic dissection. Diagnosis is confirmed by computed tomography, aortography or echocardiography. A 62-year-old man without a history of hypertension, emphysema or congestive heart failure visited our hospital because of hoarseness which developed suddenly during the physical strain. A contrast material-enhanced computed tomographic (CT) scan was performed at an other hospital due to hoarseness, mild fever and general weakness. Then he visited to our ENT department and performed laryngeal CT due to hoarseness. The CT shows somewhat mass-like soft tissue, so he was transferred to our internal medical department. The chest CT scan and simple chest PA was done and we found a dissecting aneurysm. This case developed by Physical strain and diagnosed by hoarseness is unusual and rare.

최소차 하천의 임계면적에 따른 유역 지형매개변수의 변화특성

안승섭,박노삼,김종호,임기석,송시훈 한국환경과학회 2005 한국환경과학회지 Vol.14 No.1

The size of minimum order stream has a very sensitive effect on runoff analysis model using the divergence characteristic of stream. Therefore, in this study, the threshold area of minimum order stream has been examined the change characteristic of topographical parameters. The subject basin of the research was the upper basin of the Kumho water gage station which is located in the middle of the Kumho river. The 1:25,000 numerical geography which was constructed 10×lOm mesh was used. The range of investigation of topographical parameters are number of stream order, length, area, slope, basin relief, sinuosity ratio, drainage density and total stream length etc. It was found from the result of analysis that the threshold value of minimum order stream has a very big effect on topographical parameters of basin. It was found that the threshold area of minimum ord er stream revealed under 0.10km². Furthermore, the parameters showed a serious change except for over 0.10km².

류마티스성 승모판 협착증의 치료를 위한 경피적 승모판 성형술의 치료성적

김성은,조흥근,박성훈,박시훈 梨花女子大學校 醫科大學 醫科學硏究所 1999 EMJ (Ewha medical journal) Vol.22 No.4

연구목적 : 류마티스성 승모판 협착증에 대한 유용한 치료법인 경피적 승모판 성형술의 본원의 단기 치료 성적을 알아보고자 하였다. 방법: 1993년 10월에서 1999년 4월까지의 이대목동병원에 내원한 류마티스성 승모판 협착증 환자 21명에서 투시조명 하에 경피적 승모판 성형술을 시행하였다. 시술전에 경흥부심초음파와 경식도초음파가 시행되었고, 시술후에는 경흉부초음파를 사용하여 혈역학적, 임상학적 변화를 비교하였다. 연구대상의 거의 모두가 NYHA class Ⅱ에 속했고, 5명이 심방세동을 가지고 있었으며, 모두 심초음파점수가 8이하였다. 좌심방에 혈전이 있는 경우로 혈전을 용해시킨 후 시술한 경우가 2예였다. 결과 : 경피적 승모판 성형술 이후의 평균 승모판구면적은 시술전의 1.16±0.36㎠에서 2.06±0.33㎠로 증가되었다. 경승모판 이완기 평균 압력차는 시술전 11.60±5.54mmHg에서 시술 후 4.93±2.53mmHg(p<0.001). 좌심방 크기는 46.41±14.66mm에서 42.03±15.01mm로(p=0.042), 그리고 심박출량은 4.21±1.25L/min에서 6.88±9.57L/min로(p<0.0001) 의미있는 호전을 보였다. 3도 이상의 심한 승모관폐쇄부전이나 심각한 시술후 합병증은 없었다. 결론 : 본원에서 약 6년간 시행된 경피적 승모판 성형술는 그 사례가 적고 시술전 예상되는 난이도 면에서 특이한 것은 없었지만, 시술후 합병증이 거의 발견되지 않았으며 혈역학적 측정상에서도 성공적인 시술을 보여주었다. 승모판구면적에 따른 시술후 단기내 호전의 정도는 거의 예측할 수 없었으나, 심초음파점수는 모두가 8점이하로 높은 성공율의 조건을 제공하였다. Objective : Percutaneous mitral valvuloplasty(PMV) became a treatment modality or mitral stenosis because of its low morbidity, short hospital stay, and low cost. We reviewed clinical and hemodynamic results after PMV for the patients with mitral stenosis in Ewha Womans University Mokdong hospital. Methods : We compared the results of echocardiographic, hemodynamic, and clinical parameters before and after PMV. PMV was performed under fluoroscopic guidance in 21 patients(M:4, F:17, mean age 43±12 years) with mitral stenosis from October 1993 to April 1999. Transesophageal echocardiography(TEE) and Transthoracic echocardiography(TTE) were performed for the evaluation of mitral valve, chamber size, and the presence of left atrial thrombus before procedures. TIE was also used for follow-up evaluation. On presentation, all patients showed at least NYHA class Ⅱ. Five patients had atrial fibrillation. Two patients with thrombus in the left atrium were included to study group after thrombolytic treatment with coumadin. Echo-score of our patients was not greater than 8. Results : Mean mitral valve area(MVA) by 2 dimensional or Doppler echocardiography was increased from 1.16±0.36㎠ before PMV to 2.06±0.33㎠ after PMV. There were marked improvements in transmitral gradients(11.60±5.54mmHg before PMV vs 4.93±2.53mmHg after PMV, p<0.001), left atrial dimension(46.41±14.66mm vs 42.03±15.01mm, p=0.042), and cardiac output(4.21±1.25L/min vs 6.88±9.57L/min, p<0.0001) following PMV, Severe(≥GⅢ) mitral insufficiency or severe postprocedural complications were not noted. This suggested that all procedure was successful. Conclusion : The Procedural success rate of PMV in Ewha Womans University Mokdong hospital was 100%. Low echo score of our patients might explain this high procedural success rate. Long-term-follow-up is warranted in the near future.