Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

Determination of Major Phenolic Compounds of Grape Juices and Wines with Different Geographic Origin

Seog-Won Chang,Nam-Sub Shin,Hwa-Ran Ka,Ki-Yeol Lee,Hyun-Ju Kim,Yong-Taek Rho 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.10

Bi-Sn 용융합금주입 목재복합체의최적제조조건 및 물리,기계적 특성

박계신 ( Kye Shin Park ),이화형 ( Hwa Hyoung Lee ),강석구 ( Seog Goo Kang ) 한국목재공학회 2014 목재공학 Vol.42 No.6

용융점이 138℃인 Bi-Sn 혼합합금 주입방법을 이용한 용융합금주입목재복합체의 제조를 통해 목재의 단점인 치수안정성과 내구성의 개선을 하고자 3가지 주요 침·활엽수 수종의 수종별 최적주입조건을 구명하고, 주입에 따른 금속주입목재복합체의 중량증가율, 열전도도, 전기저항 등의 물리적 성질과 기계적 성질을 실험하여 다음과 같은 결과를 얻었다. 1. 금속주입목재의 최적처리 조건은 185℃, 진공시간 10분 조건하에서 radiata pine의 경우 10 kgf/cm2 가압시간 2분30초, red oak는 30 kgf/cm2, 가압시간 10분, white oak는 50 kgf/cm2, 가압시간 10분에서 최적의 주입조건을 나타냈다. 2. 금속주입목재복합체의 외관적 특징은 전체적으로 재색은 회백색을 나타냈으며, 목재의 무늬를 그대로 유지하고 있다. 3. 침엽수 수종인 radiata pine은 저온용융합금주입처리로 인해 중량증가율은 12배, 밀도는 6.13 g/cm3으로 15배나 증가하였으나, 인테리어용으로 주로 사용되는 red oak와 white oak 등의 활엽수의 경우 목재해부학적 특성으로 의해 radiata pine보다는 낮은 5∼6배의 밀도증가율을 나타냈다. 4. 주입된 목재는 침·활엽수 모두 금속주입으로 인해 수분흡수율과 두께 팽윤율이 현저히 감소하였고, 매우 높은 치수 안정성을 나타냈으며, 금속주입목재복합체의 휨강도, 휨영계수, 경도 및 전기전도도와 열전도도 등도 무처리목재보다 매우 크게 향상되었다. In order to improve the dimensional stability and durability of wood, this study attempted to impregnate bismuth(Bi) - tin (Sn) alloy metal with low melting temperature into solid woods of three species such as radiata pine, redoak and white oak, and investigated to determine an optimum condition of manufacturing the metal alloy-wood composites with natural wood grains. These Bi-Sn alloys were chosen for this study because they were harmless to human and melting at low temperatures. The composites resulted in high dimensional stability and low thickness swelling, and also showed much improved performance such as high bending strength, high hardness, high electric conductivity, and high thermal conductivity as floor materials. A proper impregnating condition of all specimens was determined as 10minutes of the preliminary vacuum time, and 185℃ of the heating temperature. The proper processing condition for radiata pine wood was 2.5 minutes of the pressuring time at the pressure of 10 kgf/cm2. For red oak wood, 10 minutes of the pressuring time at the pressure of 30 kgf/cm2 were the proper condition. The proper manufacture conditions for white oak wood was determined as 10 minutes of the pressuring time at the pressure of 50 kgf/cm2.

Hepatic Stem Cell-Like Subtypes of Hepatocellular Carcinoma Revealed from the Integrative Multi-Omics Analysis Using Developmental Hierarchies

( Sung Hwan Lee ),( Bo Hwa Sohn ),( Yun Seong Jeong ),( Ji-hyun Shin ),( Ju-seog Lee ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Hepatocellular carcinoma (HCC) is lethal malignancy showing high relapse rates after curative resection in early- stage. Aggressive tumor biology in resectable HCC remains unclear. Methods: Using human fetal liver signatures, Multi-omics dataset from multiple clinical HCC cohorts were analyzed comprehensively to reveal molecular mechanisms for HCC stemness as well as potential biomarkers to enhance therapeutic efficacy for molecular targeted therapy or immunotherapy in stem celllike HCC subtypes. Results: The patients predicted to the hepatic stem cell (HS) subtype showed aggressive tumor features including large tumor size, high AFP, vascular invasion, and extrahepatic metastasis as well as worst prognosis with early recurrence even in early-stage. The oncogenic pathways in terms of cell cycle, epithelial-mesenchymal transition, and TGF-beta pathway were highly upregulated in the HS subtype. Higher mutations of TP53, RB1 with PTEN deletion were significantly identified in the HS subtype. We also identified subtype-specific tissue and serum biomarkers. Predicted responders for immunotherapy were significantly lower in stem cell-like subtypes due to higher accumulation of TAM and MDSC. The HS subtype showed potential higher response to multi-tyrosine kinase inhibitors, especially sorafenib and lenvatinib. Conclusions: Stem cell-like HCC is not only associated with a significantly higher relapse rate after curative resection but also with molecular biology for the aggressive subtype of HCC. We identified subtype-specific serum and tissue biomarkers for the stem cell-like subtypes and precise therapeutic strategies for each subtype regarding immunotherapy and molecular-targeted treatment. Our findings may offer the theoretical foundation of biomarker-based clinical trials for new therapeutic approaches to resectable early-stage HCC patients.

Cytological Features That Differentiate Follicular Neoplasm from Mimicking Lesions

Han, Kanghee,Ha, Hwa-Jeong,Kong, Joon Seog,Kim, Jung-Soon,Myung, Jae Kyung,Koh, Jae Soo,Park, Sunhoo,Shin, Myung-Soon,Song, Woo-Tack,Seol, Hye Sil,Lee, Seung-Sook 대한병리학회 2018 Journal of Pathology and Translational Medicine Vol.52 No.2

<P><B>Background</B></P><P>It is difficult to correctly diagnose follicular neoplasms (FNs) on fine-needle aspiration cytology (FNAC) because it shares many cytological features with other mimicking lesions. The aim of this study was to identify the cytological features that differentiate FNs from mimicking lesions. </P><P><B>Methods</B></P><P>We included the cytological slides from 116 cases of thyroid FN diagnosed on FNAC, and included their subsequent histological diagnoses. We evaluated the cytological architectural pattern and nuclear features of the lesions according to their histological groups. </P><P><B>Results</B></P><P>The final histological diagnoses of the 116 cases varied, and included 51 FNs (44%), 47 papillary thyroid carcinomas (40%) including follicular variant, and seventeen cellular nodular hyperplasias (15%). Regardless of the final histological diagnosis, microfollicular pattern was observed in most cases. On the other hand, trabecular pattern was identified in 34% of FNs, but not in any other lesions. Additionally, elongated nuclei and ground glass chromatin were found in only some papillary thyroid carcinomas. </P><P><B>Conclusions</B></P><P>This study shows that the trabecular pattern is a representative cytological feature of FNs that can be used to distinguish FNs from mimicking lesions. In addition, nuclear shape and chromatin pattern can be used to further confirm the diagnosis of FNs from mimicking lesions through FNAC.</P>

Clinically relevant stem cell-like subtypes revealed from integrative multi-omics analysis using developmental hierarchies of the liver: Precision strategies for an aggressive subtype in resectable early-stage hepatocellular carcinoma

SUNG HWAN LEE,Bo Hwa SOHN,Yun Seong JEONG,Ji-hyun SHIN,Ju-seog LEE 대한외과학회 2019 대한외과학회 학술대회 초록집 Vol.2019 No.10

품종별 고구마 전분의 이화학적 특성

김준평,석호문,신동화,남영중,박용곤 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.2

The physicochemical properties and charteristics of sweet potato starches which were isolated from the six varieties were investigated. The shapes of starch granules which observed through photomicroscope and scanning electron microscope lucre round and polygonal, but those of the Shinmi were most polygonal, and the average diameters were in the range of 10.4∼14.2 microns. The amylose contents were between 25% and 28%, and blue values and alkali numbers were in the range of 0.29∼0.36, 7.0∼12, respectively. The swelling power and solubility patterns of the starches were negligible until 50℃, thereafter it increased rapidly and the Eunmi showed highest water binding capacity of 211.6%. Amylogram pattern of 6% starch solutions were similar to no peak viscosity, but maximum viscosity varied widely with varieties. A significant positive correlation was observed between amylose content and average gelatinization temperature. Taste and texture of the steam cooked sweet potatoes were negatively and positively correlated with moisture and amylose contents, respectively, while those of the microwave cooked sweet potatoes were only positively correlated with amylose contents.

The Prevalence and Awareness of Hypertension and the Relationship between Hypertension and Snoring in the Korean Population

(Jin Seok Kim),(Woo Hyuk Song),(Chol Shin),(Chang Gyu Park),(Hong Seog Seo),(Wan Joo Shim),(Dong Joo Oh),(Sae Hwa Ryu),(Young Moo Rho) 대한내과학회 2001 The Korean Journal of Internal Medicine Vol.16 No.2

N/A Background: Hypertension is the most important, and yet modifiable, risk factor for cardiovascular diseases. But in many countries, hypertension remains poorly controlled. Moreover, sleep apnea syndrome has shown that it is correlated with hypertension. The purpose of this study was to investigate the prevalence, awareness and control of hypertension among the Korean people and to evaluate the relationship between hypertension and snoring. Methods: A total of 640 subjects living in Ansan, a regional city in Korea, were selected randomly, and trained nursing students investigated their age, sex, medical history, blood pressure, body mass index (BMI) and snoring score. Blood pressures were measured three times with a 10 - minute interval and then averaged. The degree of snoring was estimated using a questionnaire. We divided the subjects into hypertensive (BP≥140/90 mmHg) versus normotensive group and snorer versus non-snorer group, and correlated hypertension with snoring. Results: Of 640 subjects, 311 were male. The mean age was 39.7±14.6 years (18-77 years), the mean BMI (body mass index) was 22.4±3.0 kg/m2. The mean systolic and diastolic blood pressure was 121±15.7 mmHg and 79.5±11.6 mmHg. The prevalence and awareness of hypertension were 22.2% and 16.9%, respectively, and the prevalence of snoring was 35.2%. With the increment of age, in the male, the prevalence of hypertension and snoring were higher, and the snorer group showed a higher risk of hypertension than the non-snorer group (Odds ratio 2.32, CI=1.56-3.39, p=0.0001). Conclusion: In Korea, the prevalence of hypertension was similar to that in the western countries, but the awareness of hypertension was much lower compared with western countries. The prevalence of hypertension was higher in the snorer group, so more research on the correlation between the two conditions should be advanced in the future.

제분방법별 쌀가루의 이화학적 특성

박용곤(Yong-Kon Park),석호문(Ho-Moon Seog),남영중(Young-Jung Nam),신동화(Dong-Hwa Shin) 한국식품과학회 1988 한국식품과학회지 Vol.20 No.4

Lewis 폐암 마우스 모델에서 Interleukin-12가 E-selectin 발현에 미치는 영향

이상학 ( Sang Haak Lee ),신윤 ( Yoon Shin ),윤형규 ( Hyoung Kyu Yoon ),이숙영 ( Sook Young Lee ),김석찬 ( Seok Chan Kim ),권순석 ( Soon Seog Kwon ),김영균 ( Young Kyoon Kim ),김관형 ( Kwan Hyoung Kim ),문화식 ( Hwa Sik Moon ) 대한결핵 및 호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.47 No.2