Nitric Oxide에 의한 난소 과립세포의 Apoptosis 조절에 대한 연구

강한승,이석자,김외리,이채관,신창숙,김성태,강성구 인제대학교기초과학연구소 1998 자연과학 Vol.2 No.-

여포의 퇴화가 진행되는 기작에서 apoptotic cell death 와 nitric oxide (NO)의 연관성에 관하여 실험하였다. 난소의 apoptosis는 GnRH에 의해 유도되고 스테로이드에 의해 억제된다. 여포에서 과립 세포의 apoptosis에 대한 호르몬의 영향과 NO 생산효과를 조사하기 위하여 중간크기 (직경 5-6 ㎜)의 여포에서 non-apoptotic 과립세포를 분리하여 스테로이드, GnRH agonist, GNRH agonist+SNP, 그리고 NO 공여체인 SNP를 처리하여 24시간동안 배양하였다. 배양된 과립세포에서 게놈 DNA를 추출하여 apoptotic DNA절편화 경향을 알아보았고, 배양액과 griess reagent를 반응시켜 nitrite 농도를 측정하였다. 스테로이드 호르몬을 처리하였을 때, 대조군에 비해 NO 생성이 다소 증가하였으며 apoptotic DNA 절편화도 억제되었다. GnRH agonist 처리군에서는 apoptotic DNA 절편화가 가속화 양상을 나타내었으며 NO 생성은 다소 감소하였다. 그러나 GnRH agonist+SNP 그리고 SNP 처리군에서는 apoptotic DNA 퇴화가 억제되었으며 NO 농도는 대조군에 비해 현저히 높았다. 위의 실험 결과로 보아 과립세포 분화와 여포성숙과 퇴화과정에서 호르몬과 NO가 밀접한 관련이 있는 것으로 사료된다. To study the regulation of porcine follicular cell apoptosis by steroids, GnRH agonist and nitric oxide, we have analyzed DNA fragmentation, the hallmark of apoptosis, and nitrite production in porcine granulosa cells. In, ovarian apoptosis is induced by GnRH but is prevented by steroids. Apoptosis is confined to the granulosa cells but it was not clear whether apoptosis of granulosa cell was regulated by NO in vitro. So non-apoptotic granulosa cells isolated, grown at 37℃, for 24 hrs, and treated with / without SNP, GnRH agonist, and sterioid, respectively. In cultured granulosa cells were used to extract genomic DNA and culture media was assayed for nitrite concentration. Nitrite production of culture media was increased and apoptotic DNA fragmentation was supressed in SNP treated groups, GnRH agonist+SNP, and estrogen, progesterone. Nitrite concentration in culture media was decreased and apoptotic DNA was induced treated with GnRH agonist. These data suggest that NO production and apoptosis may be involved of granulosa cell differentiation and ovarian follicular atresia by SNP, estrogen and progesterone. Moreover, NO can overcome the granulosa cell apoptosis induced by GnRH agonist.

Cu 촉매하에서 유기주석 화합물과 고분자에 결합된 Iodide의 Cross-Coupling 반응에 관한 연구

강석구,임권호,김재선,윤석근,윤승수 성균관대학교 기초과학연구소 1996 論文集 Vol.47 No.2

The copper iodide-catalyzed cross-coupling of polymer bound aryl iodide with organostannanesin N-methyl-2-pyrrolidone (NMP) was accomplishied smoothly in the presence of NaCl.

고혈압 관리를 위한 의사지원결정시스템의 데이터 마이닝 접근

강석민,조승연,채영문,김태수,호승희 대한간질학회 2003 대한간질학회지 Vol.9 No.2

RFID 표준 미들웨어 구현을 위한 고찰

강승석 서울여자대학교 2010 정보기술논문지 Vol.8 No.-

RFID is an effective automatic identification technology for a variely of objects including humans, animals, and artifacts, RFID has already found its way into a diverse successful business computing technology, The RFID applications spans from the business sectors to the public facilities to even daily activities using mobile services. In general, RFID system has four components including tags, reader devices, a middleware, and applications, EPC global has proposed the standard specifications for the components and the interfaces between them. ALE (Application Level Event) is a de facto standard fo the RFID middleware defined by the EPC global. This paper explains the four components in detail and emphasizes especially on the ALE v1.1 which contains five groups of APls. In addition, this paper includes the ALE logical model of the internal structure and the consideration of the implementation process. This paper hay help understanding the standard RFID system functions and implementing the model for the enhanced ALE v1.1 standard.

韓國의 Ombudsman制 適用化 硏究

姜錫勝 단국대학교 대학원 1980 學術論叢 Vol.4 No.-

Prediction is a risky, difficult, and unrewarding activity in any time, and forecasting social trends even more So. To engage in such an endeavor in a world of unprecedented complexity during changes of unparalleled rapidity is as absurd as it is necessary. We will argue that democracy has been So widely embraced not because of some vague yearning for human rights but because under certain conditions it is a more "efficient" form of social organization. We do not regard it as accidental that those nations of the world that have endured longest under conditions of relative wealth and stability are democratic, while authoritarian regimes have, with few exceptions, either crumbled or maintained a precarious and backward existence. I have discussed in this article the system of ombudsman, particularly accentuating adaptation of ombudsman System in Korea. This thesis is aimed at making treatise research and comparative methods. The trends of world events in the twentieth century is toward the dominance of super governments that are large in scale, top-level, and power-ful. Correspondingly, ombudsman is fashionable in the world, especially in Scandinavia countries. Because ombudsman is a protector of citizen's complains and is a sort of external control, which derived from Sweden in 1809. Ombudsman is originally appointed for term of parliament and may be removed by the parliament in Sweden. But Ombudsman is appointed by the president acting on the recommendation of the prime minister after consultation with the leader of the opposition and may be removed or suspended by the Governor-General upon an address from the House of Representatives for disability, bankrupcy, neglect of duty, on misconduct in new Zealand. Thus, Ombudsman is propagated to other nations in the world at present times. There are many methods of adapting ombudsman system as enumerated below in Korea. 1) The Office of Inspection (Gam-sa-won) 2) Legal relief systems of civil complains 3) The system of administrative counselling 4) The System of Petition Rights 5) Private relief systems, etc. The choice of any one of these methods varies, of course, according to state and local community even in the same state, as like U.S.A. I think that the best way to adapt ombudsman system in korea is; 1) Public relations of executive authorities have to be more increasing than before. 2) Make away with spoils characteristics in government dimensions. 3) Political education of democracy should be put one's knowledge to practical use for the people. 4) Establishment of new organizations which have ombudsmanic naturesis necessary to Governmental units except from overlapping functions that have existing systems. I have analyzed those various hindering elements as mentioned above and want close this thesis, hoping that decisive adaptation of ombudsman system be made within shortest time.

버스 정차시간 분석

강영균,서준석,고승영 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

The time spent by passengers to board and alight buses is an important component of the total bus travel time. On average, about one fifth of the total travel time of a bus is spent at bus stop. If this stopped time due to boarding and alighting could be reduced, the saving of total travel time would be reduced also, and this contributes to the efficiency of route operation and vehicle fleet usage. So, this paper defined several kinds of times which are measured in this research of passenger boarding time, alighting time and acceleration - deceleration time. Also, this paper developed a model for bus boarding and alighting time as a function of factors such as fare system, vehicle design and the difference of travel time, and to get the value of bus acceleration -deceleration time in particular factors such as vehicle design and bus stop design.

鐵 陽極 溶出反應에 의한 燐成分 除去

강민수,김형석,박승조 동아대학교 환경문제연구소 1995 硏究報告 Vol.18 No.1

This study was on the removal of phosphorus compounds in domestic and synthetic wastewater using iron-anode electrolytic reactor. The experiments were performed at different current, reaction time and flow rate. The results are as follows ; 1. For the synthetic wastewater with the flow rate of 1.5 ℓ/min and 0.75 ℓ /min, the removal efficiencies were 97% and 98%, respectively. The order conditions of this continuous experiments are current density 2.54 A/dm² and reaction time 20 minutes. 2. The optimal conditions of the batch experiment for synthetic wastewater were current density 0.95 A/dm² and reaction time 3.0 minutes with no addition of electrolyte. With electrolyte, the optimal conditions were current density 2.54 A/dm² and reaction time 1.0 minutes. For the both experiments, the removal efficiencies were far over 97%.

압축력과 휨을 받는 Hollow Core Slab의 구조적 거동에 관한 연구

강원석,김승훈,이리형,김용남,김종수 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Recently, Many engineers have used Hollow Core Slab(HCS) system on buildings. However, they couldn't apply this system to under three underground buildings, because the axial force effect hasn't yet been clearly investigated. This paper presents the test results for structural behaviors of HCS under axial and bending load. Variables are the thickness of Topping concrete and the maginitude of axial forces. The thicknesses are 100mm and l50mm. The axial forces are 0kN, 911.4kN, 1313.2kN, 1640.5kN. The results showed that HCS system has more strength capacity as the axial forces are increaser.

난원공 개존을 통한 우심방에서 좌심방으로의 단락 진단에 있어 경흉부 심초음파 하모닉 영상의 유용성

신미승,강석민,장길진,변기현,하종원,정남식,허지회,이병인 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

부산시 가로수 현황과 토양환경개선에 관한 연구

김석규,박승범,남정칠,김승환,강영조 동아대학교 건설기술연구소 1999 硏究報告 Vol.23 No.2

The purpose of this study is to analyze the correlation between soil hardness, soil acidity, and the vitality of street trees. This data was investigatived in 40 areas on 10 main street routes of Pusan; Chungang-ro, Naktong-ro, Suyeong-ro, Taejong-ro, Konghang-ro, Kudeok-ro, Kumjeong-ro, Mandeok-ro, Kaya-ro, Chungjang-ro. The findings are as follows : 1. The average soil hardness where tree grates have been built was shown to be 22.23㎜. In the absence of tree grates, the average soil hardness was 24.60㎜. These figures do not exceed the accepted parameters for acceptable soil hardness when rearing trees (22∼25㎜). However, soil hardness was measured higher in areas where tree grates were absent. Consequently, tree grates are necessary. 2. Soil acidity was measured as satisfactory(pH 5.5), mediocre(pH 5.5∼5.0), inferior(pH 5.0∼4.5), very inferior(pH 4.5). No areas were measured as satisfactory soil acidity. Four areas were measured as mediocre(10%), 19 areas as inferior(47.5%), and 17 areas as very inferior (42.5%). This indicates a requirement to improve soil acidity as about 90% of the investigatived areas were measured as either inferior or very inferior. 3. The following is an analysis of correlation between soil hardness, soil acidity and the vitality of the Ginkgo biloba ; The Ginkgo biloba was found to have a positive correlation was measured with the diagram (R²). In conclusion, the results of the analysis show a strong influence of soil hardness and soil acidity upon the vitality of Street Tree.