Effective Combination of Agrobacterium tumefaciens Strains and Ti Plasmids for the Construction of Plant Vector System

Kim, Mi Suk,Park, Jeong Ku,Eum, Jin Seong,Sim, Woong Seop 한국식물학회 1996 Journal of Plant Biology Vol.39 No.3

The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transformed with four kinds of Ti plasmids, pTiBo542, pTiA6, pTiKU12 and pTiAch5, respectively. The stems of 28 species of dicotyledonous plants were then inoculated with these transformants and examined for crown gall formation. The different combination of A. tumefaciens strains and Ti plasmids showed quite a difference in terms of the crown gall formation. Agrobacterium strains A136 and KU12C3 have a same plant host range in case that both strains barbour the same kind of Ti plasmid, pTiBo542 or pTiAch5. However, the above-mentioned both strains have quite different host range in the event of containing the same Ti plasmid, pTiKU12 or pTiA6. In case that KU12C3 contains pTiA6 or pTiKU12, this strain has a wider plant host range than A136. The plant host range of pTiBo542 is the widest, followed by pTiA6, pTiKU12 and pTiAch5. Twelve plants among 28 tested plants are not transformed by any virulent Agrobacterium strains used in this study. In conclusion, A. tumefaciens KU12C3 and A136 harboring pTiBo542 showed the widest host range for transforming dicotyledonous plants. Also, it was acertained that the host range of Ti plasmids is affected by chromosomal level.

소아에서 발생한 골수이형성 증후군 2례

홍성진,윤석중,김성우,조형구,오명호,김기혁,이인성 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.5 No.-

Myelodysplastic syndrome(MDS) is a group of acquired conditions characterized by progressive bone marrow failure associated with normocellular or hypercellular bone marrow that cannot be attributed to nutritional deficiency, chronic infection, or other chronic systemic illness, and cannot be reversed by the successful treatment of those conditions. Whereas the cases of secondary MDS occur at all ages, primary MDS is essentially a disease of elderly, though no age group is exempt and predomonant in men. The MDS is fundamentally clonal disorders at the level of the haemopoietic totipotent stem cell. Clinically, they are characterized by varing degrees of peripheral cytopenia(s) with morphological and functional abnormalities of blood elements. We have experienced two unrelated pediatric cases, who showed pancytopenia on admission and the characteristic marrow findings of MDS. The first case, a 12 months-old girl, showed increased reticulin with abnormal localization of immature precursor cells(ALIP) in her bone marrow, in addition to dysplastic features such as bi-and multi-nucleation of late normoblasts and internuclear bridging. She also showed 22% of blasts in her bone marrow cells thus classified in the category of RAEB-t after FAB classification. Also found were a chromosomal abnormality namely 46 XX t(14q21q), which was an unusual one. Her family brought her home and, 4 month later, she deceased. The second case, a 12 year-old boy, showed pancytopenia with Pseudo-Pelger-Huet anomaly in neutrophils but no blasts in the peripheral blood. Bone marrow of this case also showed the picture of dyserythropoiesis with binuclearity, internuclear bridging, nuclear fragments, multinuclearity of late normoblasts, in addition to megaloblastoid change in the normoblasts and increased mitosis in granulocytic series. No chromosomal abnormality was found. Classified as RA after FAB classification of MDS. Showed good response to conservative management for anemia for more than one year.

햄릿 공연을 위한 무대의상 디자인 (Ⅰ) : 관객을 통한 인물이미지 분석 The Analysis of Actor Image by Spectator

김순구,황성원 한국의류산업학회 2004 한국의류산업학회지 Vol.6 No.1

This study is done to assist in the limit of stage dress design research considering the sense of the times and the sympathy of spectators for actual stage. For the base research, to visualize the image which the spectators are feeling from Shakespeare's Hamlet, the evaluators had to analyze the whole image and color tone of the figures. First of all, 2 clothing for Hamlet, Gertrude and Ophelia had to be designed, and following conclusion has drawn. Hamlet A corresponds with passive looks which is a combination of loneliness, sadness and the unstable mental state of not adapting to and hiding from the reality. Hamlet B has more of matured looks which make Hamlet more free and comfortable from life and death as he overcame a crisis of death. Gertrude A corresponds with the image of coming right out of the funeral of late king. Gertrude B shows more of her sadness as she hears of her son Hamlet's despair and resentment and her maternal return degradation which is a special situations emphasizing her unstable and dizzy image. Ophelia A shows her usual and normal looks of a livelihood and femininity. She congratulates the wedding and coronation, and falls in love with Ham-let which shows a lot of her girlish image. Ophelia B shows dismantled rationality and violated virginity which is a very complex and unstable state showing a completely different image. Based on the above research. It was proposed their clothing through the next study of the characters, and personally make them for 2003 Yeonheedan Street Group's performance Hamlet.

내측연수경색에서 관찰된 안구의 반대편 측방돌진

김지수,문소영,정선미,한문구,박성호 대한평형의학회 2003 Research in Vestibular Science Vol.2 No.2

가창학습시 계명창과 가사창의 상호 우선학습의 장단점에 대한 고찰 : Centered on the 6th Grade Students

김성복,양순구 晋州敎育大學校 初等敎育硏究所 1993 初等敎育硏究 Vol.3 No.-

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

율무 종자의 크기 및 溫度에 따른 幼苗의 生長 特性

윤성탁,김봉구 단국대학교 1998 論文集 Vol.32 No.-

This study was conducted to investigate seedling characteristics of Job's tears according to the seed size and different germination temperatures in order to find out the method to improve the seedling vigor and uniform establishment of Job's tears(Coix lachryma-jobi L.) in the field. The results obtained were summerized as follows. 1. In the 100 grain weight of seeds of two varieties. Yunchoon 9 ho was 14.2g, which was 2.4g more higher than that of Yulmoo 1 ho. 2. In the percentage of emergence of two varieties. Yunchoon 9 ho was 81.4% that was 7.4% more higher than that of Yulmoo 1 ho and small seed size was 6% more higher than large seed between seed sizes. 3. In the percentages of germination under different temperatures, 25℃ plot was 39.7%, which was lowest and 30℃ and 35℃ plots showed the same percentage of 96.7%. Accordingly it is judged that from 30℃ to 35℃ is advisable to ensure uniform emergence. 4. Days from seeding to emergence was not shown different between two varieties, but it was shown significantly different among seed size and temperature treated. Days from seeding to emergence of large seed size was 6.7 days which is 0.6 day shorter than that of 7.1 days of small seed. Among temperatures treated, 25℃, 30℃ and 35℃ were respectively and it was observed that 35℃ plot showed the shortest in days from seeding to emergence. 5. Dry weight of plant at 20 days after seeding among seed sizes and temperatures was shown statistically difference. Large seed was 38.0㎎ that is 8㎎ higher than 30㎎ of small seed. Dry weight due to the germinating temperatures of 25℃, 30℃ and 35℃, 35℃ plot showed 42.3㎎, which is highest compared with other two temperatures of 30.5㎎, and 29.0㎎ respectively. 6. Chemical composition of seeds in the content of moisture, ash and crude fat were not significantly different, but content of crude protein was significantly different.

X-cut LiNbO_3 광도파로 제작 및 패턴 특성

정운조,김성,박계춘 국립7개대학공동논문집간행위원회 2001 공업기술연구 Vol.1 No.-

The optical near field patterns, propagation loss and mode sizes of x-cut Ti:LiNbO_3 optical waveguide which was fabricated by Ti-diffusion varying with Ti strip thickness in wet oxygen atmosphere were investigated and tested at optical wavelength 1550㎚. As Ti thickness increased from 760A˚, the insertion loss of waveguide was decreased. But at Ti thickness 1500A˚, mode sizes are widely broadened. The Ti thickness of below 1100A˚ and above 1500A˚ showed negative effects to propagation loss and fiber coupling. The best Ti thickness for fabricating low propagation loss and good fiber coupling was inferred to be between 1100A˚-1500A˚ in our conditions. And for Ti thickness 1150A˚, its propagation loss, horizontal/vertical mode sizes were measured 1.61㏈/㎝, 11.9/8.9㎛ for TM, 0.22㏈/㎝, 12.0/9.1㎛ for TE respectively.

X-cut LiNbO_3 광도파로 제작 및 패턴 특성

정운조,김성,박계춘 木浦大學校 應用科學硏究院 2001 應用科學硏究誌 Vol.1 No.-

The optical near field patterns, propagation loss and mode sizes of x-cut Ti:LiNbO_3 optical waveguide which was fabricated by Ti-diffusion varying with Ti strip thickness in wet oxygen atmosphere were investigated and tested at optical wavelength 1550nm. As Ti thickness increased from 760Å, the insertion loss of waveguide was decreased. But at Ti thickness 1500Å, mode sizes are widely broadened. This Ti thickness of below 1100Å and above 1500Å showed negative effects to propagation loss and fiber coupling. The best Ti thickness for fabricating low propagation loss and good fiber coupling. The best Ti thickness for fabricating low propagation loss and good fiber coupling was inferred to be between 1100Å-1500Å in our conditions. And for Ti thickness 1150Å, its propagation loss, horizontal/vertical mode sizes were measured 1.61dB/cm, 11.9/8.9㎛ for TM, 0.22dB/cm, 12.0/9.1㎛ for TE respectively.

결절성 및 괴상성 간세포암에서 역동적 전산화단층촬영 소견에 따른 경동맥화학색전술결과의 예측

이성문,김홍,지성우,우성구,이정형,서수지,손철호 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.2

Purpose: To evaluate retrospectively whether it is possible to predict the transarterial chemoembolization(TACE) result with the pre-TACE dynamic CT findings. Materials and Methods: The dynamic CT findings of 33 patients with 35 nodular and 8 massive hepatocellular carcinoma(HCCs) who was taken follow up CT after TACE, were reviewed retrospectively. After excluding the diffuse or infiltrative HCCs, the mass was classified into two groups of nodular and massive types. The size, location, degree of enhancement, pseudocapsule, portal vein(PV) thrombosis and arterioportal(AP) shunt were evaluated on the dynamic CT scans. The vascularity, multiple or parasitic blood supply, AP shunt and the possibility of superselection were considered on the angiography. After TACE, the pattern of lipiodol uptake and residual enhancing nodules were evaluated on the follow-up CT scans. Results: The good TACE results could be obtained in 18 of 35 nodular HCCs with dynamic CT findings of small size(mean=2.94cm), peripheral location, hyper-or iso-enhancement, and absence fo PV thrombosis, with angiographic finding of possible superselection, but the pseudocapsule did not give important effect on the result. Most of the massive HCCs had poor TACE results. Conclusion: The prediction of the TACE results of nodular and massive HCCs was possible with dynamic CT findings. If the good prognostic factors were not observed on the pre-TACE dynamic CT, the multiple TACE and/or combination therapy with percutaneous ethanol injection(PEI), surgical resection or others should be considered for the effective control of the HCCs.