cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

한국 대학생들의 영어능력과 어휘 반응 속도 간의 상관관계

강상구 청주대학교 인문과학연구소 2017 人文科學論集 Vol.54 No.-

This study utilized a body-part picture naming task (HALA - Hawaii Assessment of Language Access) to assess the proficiencies and dominance of Korean and English from 20 Korean college students. Their accuracy and response time were gauged to show the correlation between language proficiency and vocabulary response time. The results of this study suggest that lexical accessibility can be used as a legitimate reflection of a speaker's overall language proficiency and thus, the potential of utilizing the HALA project as a simple and convenient tool for future related research should be seriously considered.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

생활도우미활동이 장애학생에 대한 일반학생의 또래관계에 미치는 영향

강성구 국립특수교육원 2005 특수교육연구 Vol.12 No.2

이 연구는 일반중학교에서 장애학생의 학급생활을 돕기 우해 활용되어지고 있는 생활도우미 활동이 장애 학생에 대한 일반학생의 또래관계에 미치는 영향에 대해 알아보고자 하는 데 목적이 있다. 이를 위해 통합교육을 실시하고 있는 일반중학교 1개교(208명)를 대상으로 학급배치요인(장애학생과 같은 반/다른 반)과 또래관계개입요인(개입 유/무)을 기준으로 집단을 구분하고, 또래관계 질문지를 통해 또래관계를 전체 점수와 하위 점수(공유활동, 친사회적행동, 친밀감, 애착ㆍ자존심ㆍ고양, 신의, 갈등)로 환산하여 각 집단 간 차이를 분석하였다. 연구 결과는 1. 학급배치요인(physical proximity)은 일반학생과 장애학생의 친밀감을 감소시키지만 갈등도 감소시켰다. 그 효과는 미미하다. 2. 또래관계개입요인(peer-relationship intervention)은 일반학생과 장애학생의 또래관계에서 갈등을 제외한 대부분 영역에서 또래관계 점수를 높여주었다. 3. 또래관계점수는 학년에 영향을 받았고, 상호작용효과는 학년과 학급배치요인 간에 두드러졌다. 그러나 또래관계 점수는 성별에는 영향 받지 않았다. 따라서 통합교육환경에서 단순히 학급배치만을 고려하는 것은 장애학생과 일반학생의 또래관계개선에 큰 도움이 되지 못했다. 그렇지만 생활도우미활동을 실시했을 때 또래관계개입을 함께 고려하면 또래관계를 개선하는데 큰 도움이 된다는 결론을 내릴 수 있었다. The objects of this study is to find the effects of Peer helper activity which are related with the non-disable student's peer relationship with disable student. I took sampling 208 students who went to N School which progress peer-helper activity, surveying and analyzing it according to proximity, peer relationship intervention, grade, sex. The peer-helper activity effects remarkably on non-disable student's peer relationship with disable student. However, there is some question in building up the in creasing non-disable student's peer relationship with disable student which is only peer-relationship intervention. The remarkably peer-helper activity's effects is peer-relationship intervention and a chance which interact with disable students. and The grade interacts significantly with Physical proximity and has main effects but, The sex does not interact significantly with any ones and does not have main effects. Come to the Conclusion that activity which is only refer to proximity is not helpful to non-disable student's peer relationship with disable student but that activity which is refer to both Physical proximity and peer-relationship intervention is helpful to non-disable student's peer relationship with disable student.

비외상성 두개내 출혈 환자에서 심근손상의 발생과 예후에 미치는 영향

강구현,황성호,이강현,조준휘,김성환,문중범,박해상,이서영,이성수,김헌주 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Objective: The aim of this study was to investigate the clinical significance of myocardial injuries in patients with nontraumatic intracranial hemorrhage by identifying the occurrence of myocardial injury and defining its correlation with subsequent cardiovascular events. Subjects and methods: One hundred twenty-four patients with nontraumatic intracraninal hemorrhage presented to the emergency department within six hours from onset of symptoms were enrolled. Brain CT, serial electrocardiography, and echocardiography were done at the emergency center. Blood samples for troponin I and creatine kinase(CK)-MB were drawn immediately and eight hours after admission, Troponin I and CK-MB were measured using a chemiluminescent immunoassay, respectively. Results: Electrocardiographic and echocardiography abnormalities were found in 65 cases(52.4%) and 21 cases(17%), respectively. Serum troponin I and creative kinase-MB were increased in 35 cases (28.2%) and in 58 cases(46.8%), respectively. Abnormal findings of echocardiography and ECG, as well as elevated levels of serum troponin I and creative kinase-MB, were associated with an increased risk of cardiovascular event and survival. Logistic regression analysis revealed that an abnormal echcocardiographic finding and elevation of serum troponin I were factors associated with the occurrence an adverse cardiovascular event and that electrocardiographic abnormalities and initial mental status were factors associated with poor prognosis. Conclusion: This study reveals that actual myocardial injury develops in a significant proportion of patients with nontraumatic intracranial hemorrhage and that the development of the myocardial injury is associated with an adverse cardiovascular event that occurs during admission.

영어 이중목적어 구문 구사 시 한국 고등학교 영어 교사들의 담화원칙 적용 양태

강상구 淸州大學校 韓國文化硏究所 2023 人文科學論集 Vol.66 No.-

In this paper, Korean high school English teachers’ use of English dative constructions is compared with that of English native speakers, especially focusing on how the given-before-new(GN) discourse principle is applied. A written elicited production task with six dative verbs presented in each of the given-theme and ivenrecipient conditions was provided to the participants in order to probe how the two objects are aligned in English dative constructions. The results showed that Korean teachers tend to instantiate the GN order more in prepositional datives than in double object datives. This result suggests that besides their knowledge about the GN discourse principle, other factors such as the difference of difficulty in acquiring the prepositional datives and double object datives might be in play. A more in-depth research with more Korean participants at varying different English proficiency levels is required to investigate how the GN discourse principle might be instantiated by Korean learners of English.

마이크로파에 의한 WC의 합성 및 특성에 관한 연구

강성구,정윤중 明知大學校 産業技術硏究所 2005 産業技術硏究所論文集 Vol.24 No.-

Tungsten carbide(WC) powders were synthesized by self-propagating high temperature synthesis(SHS) chemical furnace that microwave heating system. Attempt to find the optimal condition for synthesis of tungsten carbide(WC), the effects of molar ratio between carbon and tungsten, compaction pressure, pellet thickness and heating time of microwave on synthesis were investigated. Powder was synthesized in Ar atmosphere. Chemical fuel was Ti-C mixed powder. Compositional and structural characterization of these powders was carried out by scanning electron microscope(SEM) and x-ray diffraction(XRD). Powder characterization was carried out by the measurement of particle size distribution with laser-particle size analyzer. Tungsten carbide(WC) obtained by microwave depend very much on the mole ratio between carbon and tungsten and heating time of microwave given in a product, i.e. Optimum molar ratio of synthesized powder was tungsten : carbide = 1 : 1.5, pellet thickness 10㎜, compaction pressure l500Psi and microwave heating time was 60sec after propagation to best condition powder. By optimizing the synthesis conditions, it is possible to fabricate tungsten carbide(WC) powders which have little secondary phases.

전해질로 나피온을 사용한 Ti/IrO_2 전극상에서 유기물의 양극산화 반응

강태구,윤영재,김희정,노성희,김선일 한국공업화학회 2003 응용화학 Vol.7 No.1

This paper describes the anodic oxidation od various alphathic alcohols in pure water using Nafion cation exchange membrane as solid polymer electrolyte. The oxidation has been carried out on an IrO_2 anode under conditions of simultaneous oxygen evolution. The experimental results for the anodic oxidation of four different alcohols are in good agreement with the theoretical model presented in a previous paper. Finally a simple experimental method is described for the determination of the relative reactivity of organics oxidation on IrO_2 anodes.

전기경련치료(ECT)시 뇌파의 비선형 분석 : 단일증례 연구 A Single Case Study

강웅구,이운철,정선주,윤세창,조맹제,이상훈,김용식 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 비선형 분석을 통해 전기경련 치료(ECT)의 적절성 및 효과를 판정할 수 있는 새로운 변인을 추출해 낼 가능성을 탐색하기 위하여 한 여성 우울증 환자에서 ECT중 기록한 뇌파의 단일증례 분석을 하였다. 방 법 : 1주일 간격을 둔 두 ECT 회기에서 치료전 안정상태, 마취후-경련전상태, 경련상태, 경련후 뇌파억제상태, 치료후 안정상태의 다섯 상태에 따른 뇌파를 디지털 기록한 데이터를 분석하였다. 결 과 : 분석의 결과 1) 뇌파가 10차원 이하 저차원의 결정론적 성질을 가지고있으며 2) 뇌파는 비정상적(non-stationary)이지만 비선형 지수들이 각 상태 뇌파를 안정적으로 구분해 줄 수 있고 3) ECT 회기의 진행에 따라 경련중 뇌파의 결정론적 성질이 증가하며 4) ECT 회기의 진행에 따라 치료전후 안정상태 뇌파의 최대발산지수(PLE) 및 상관차원이 증가한다는 사실을 관찰할 수 있었다. 결 론 : 단일증례에 대한 다양한 비선형 분석의 결과는 ECT의 치료효과를 정량화할 수 있는 변인으로서 PLE, 상관차원 등 비선형 변수들이 사용될 수 있을 가능성을 제시한다. Objectives : In order to search for new parameters for the evaluation of the adequacy of electroconvulsive therapy(ECT), the authors performed a non-linear analysis of electroencephalogram(EEG) recorded during the course of electroconvulsive therapy(ECT) in a single female patient with depression. Methods : Digitized EEG recordings from two ECT sessions spaced one week were analyzed at five different stages ; pretreatment stable, postanesthetic-preictal, ictal, postical suppression and posttreatment stable stages. Results : Our analysis showed that 1) EEG signals had low dimensional(below 10 dimensions) deterministic characteristics 2) although EEG was nonstationary signal, non-linear parameters could reliably differentiate between various stages we examined. 3) the deterministic property of ictal EEG increased according to the progression of sessions and 4) the primary Lyapunov exponent(PLE) and correlation dimension of pre- and post-treatment stable stage EEG increased according to the progression of sessions. Conclusion : In this single case study, results of various non-linear analyses suggested that non-linear variables extracted from EEG recordings during ECT may have utilities as tools for the quantitative evaluation of ECT.