말쥐치기름 및 들깨기름의 攝取가 高콜레스테롤 食餌 흰쥐의 血淸 및 肝臟의 脂質成分에 미치는 影響

鄭承鏞,朴畢淑,徐孟姬,姜晋順,禹靜淑 慶尙大學校 1985 論文集 Vol.24 No.1

Hypercholesterolemic male rats of Sprague Dawley, divided into six groups, were fed for 3 weeks with the diets containing several different oils of 10,0, the mixtures of 5% perilla oil and 5% other oils. The concentration of the lipid peroxide, T-cholesterol, phospholipid and triglyceride in the serum and liver were measured along with the HDL-cholesterol concentration of the serum. The results were as follows: 1. The concentration of LPO in the serum of the rats fed with lO% perilla oil, with 5% perilla oil+5% lard and with 5% perilla oil+5% file fish oil was higher than those with any other oils. The concentration of that was significantaly high in the liver of the rats fed with 10% perilia oil, while it was considerably low with 10% olive oil. 2. The concentration of T-cholesterol in the serum of the rats fed with 10% perilla oil, with 10% file fish oil and with 5% perilla oil+5% file fish oil was lower than those with any other oils. 3. The concentration of HDL-cholesterol was low in the serum of the rats fed with 10% perilla oil, while it was high with 5% perilla oil+5% file fish oil and with 10% file fish oil. The concen tration of that for the T-cholesterol in the serum of the rats fed with 10% file fish oil and with 5% perilla oil+5% file fish oil was much higher than those with any other oils. 4. The concentration of T-cholesterol in the liver of the rats fed with 10% perilla oil was high, while it was low with 10f file fish oil and with any other oils. 5. The concentration of PL and TG in the serum of the rats fed with 10% perilla oil and with 10% file fish oil was lower than those with any other oils, but it was lower in the liver of the rats fed with 10% perilla oil than the mixtures of perilla oil and with 10% file fish oil.

응급실에서의 확산강조 자기공명 영상

정성필,이석우,양영모,하영록,김승환,유인술 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: This study was designed to review the cases of patients who had undergone diffusion-weighted magnetic resonance imaging(DWI) in the emergency department(ED), and to investigate its clinical usefulness and current indications. Methods: We analyzed the cases of 152 consecutive patients who underwent DWI in the ED from Jan to Mar 2001. DWI was obtained with the use of a multislice, single-shot, spin-echo plana imaging technique(GE SignaR). Imaging time was less than one minute. The medical records, the DWI films and the computed topography results were reviewed. We investigated the chief complaint, initial findings of physical examination, final diagnosis, decision-making department, interval from admission to imaging, and DWI findings. Results: DWI showed positive findings of high signal intensity in 84 patients(55.3%). Among the 68 patients who yielded a negative result, false negative occurred with 12 patients(17.6%): 10 lacunar infarctions, a pons infarction, and a brainstem infarction. Eleven patients were determined as having a cerebral hemorrhage, all of whom showed the abnormal finding of a mixed signal in DWI. The sensitivity and the specificity of DWI to rule out stroke were 85.5% and 98%, respectively. Current indications for DWI in our ED are age older than 60, alert mental status, and one of the symptoms or signs among lateralyzing sign, language disturbance, and dizziness/vertigo. Conclusion: DWI was highly specific to rule out stroke, so emergency care professionals should be familiar with this new technology. Further prospective study is required to determine the proper indications and clinical usefulness of DWI in the ED.

말쥐치 기름의 投與가 흰쥐의 血淸 및 肝臟의 콜레스테롤 濃度수준에 미치는 影響

鄭承鏞,黃淑子,姜晋順,朴畢淑 慶尙大學校 1985 論文集 Vol.24 No.2

Sprague Dawley계 숫 흰쥐에 eicosapentaenoic acid 및 docosahexaenoic acid둥 고도불포화지방산을 함유하고 있는 말쥐치커기름과 monoenoic acid를 많이 함유하고 있는 올리브기름을 각각 10% 씩 첨가하고 또한 vitamin E를 식이 100g당 5, 50, 100 I. U.로 첨가한 사료로서 2주간 사육한 후 혈청 및 간장 중의 cholesterol 농도, 과산화지질 및 α-tocopherol의 농도를 측정한 바 다음과 같은 결과를 얻었다. 1. 증체량 및 사료섭취량은 전 시험군 간에 있어 유의적인 차이는 없으나 말쥐치기름 투여군이 약간 낮은 경향이었다. 2. 혈청 중의 총 cholesterol 농도는 올리브기름 및 말쥐치기름 투여 군 모두 vitamin E의 첨가량을 증가시킴에 따라 낮아지는 경향이었으며, 말쥐치기름 투여군 은 올리브기름 투여군에 비해 유의적으로 그 농도가 낮았다 (P<0.05). 3. 혈청 중의 HDL-cholesterol 농도 및 총cholesterol 농도에 대한 HDL-cholesterol 농도의 비율은 말쥐치기름 투여군이 올리브기름 투여군에 비해 대체로 높은 경향이었다. 4. 간장 중의 총 cholesterol 농도는 말쥐치기름 투여군이 올리브기름 투여군에 비해 약간 낮은 경향이었다. 5. 혈청 및 간장 중의 과산화지질의 농도는 말쥐치기름 투여군이 유의 적으로 높았으며 (P<0.05) vitamin E의 첨가량을 증가시킴에 따라 감소되는 경향이었다. 6. 혈청 및 간장 중의 α-tocopherol 농도는 말쥐치기름 투여군이 유의적으로 낮았다 (F<0.05). The Sprague Dawley male rats were fed experimental diets containing 10% file fish oil or 10% olive oil, and three graded levels of vitamin E(5,50 and 100 I.U./l00g diet) were provided for each test oil group. After two weeks feeding, the concentration of cholesterol, lipid peroxide and a-tocopherol in serum and liver of rats were determined. The results were summarized as follows. 1. The amount of body weight gain and food intake of rats were not significantly different among all the experimental groups but tended to slightly be low in rats fed file fish oil diet. 2. Total cholesterol concentrations in serum tended to be decreased as vitamin E level increased all of the file fish oil and olive oil diet groups, serum total cholesterol concentrations of the file fish oil diet groups were significantly lower than those of the olive oil diet groups. 3. HDL-cholesterol concentrations and the ratio of HDL-cholesterol to total cholesterol in serum generally tended to be higher on the file fish oil diet groups than those of the olive oil diet groups. 4. Total cholesterol concentrations in liver tended to slightly be lower on the file fish oil diet groups than those of the olive oil diet groups. 5. Lipid peroxide values in serum and liver were significantly high on the file fish oil diet groups and tended to be decreased as vitamin E level increased. 6. The concentrations of α-tocopherol in serum and liver were significantly low on the file fish oil diet groups.

패러글라이딩 사고에 대한 분석

김승환,김인병,정순미,정성필,장석준 대한외상학회 1999 大韓外傷學會誌 Vol.12 No.1

$quot;Background: Paragliding injuries are increasing recently due to the growing popularity of this sport. This topic has been discussed in numerous journals in the European countries with large mountains, such as Austria, Germany and Switzerland, Those studies gave information regarding the flight phase during which an injury ocurred, the type and site of injury, and suspected reasons for the accidents. This study was done to compare our data to those of other nations and give preventive advices to reduce injuries occurring during paragliding. Method: Nineteen paragliders documented as having injuries during paragliding at Chung-Ju, Korea from 1990 to 1997 were included in the study. Their accident reports including medical records, were analyzed retrospectively. Result: Eighteen of the injured were male with one female and their mean age was 25.6 years old. Five injuries were suffered during take-off, of which technical error was the cause in most cases. Also, 5 injuries occurred during flight due to a sudden change of thermal and wind condi- tions. The majority of injuries were suffered during the landing period, affecting 9 paragliders. They were mostly due to forced landings, unexpected obstacles, and sudden change of wind conditions. Thirty-three percent suffered spine trauma and 29% were involved in injuries of the lower extremities. Upper limb injuries occurred in 19%, and 19% also suffered cerebral concussion or contusion. Conclusion: We have found that spine and ankle injuries were commonly occur during paragliding, especially the landing phase. Proper knowledge of the procedures, adequate training and appropriate foot wear could help in reducing injuries while paragliding.$quot;

B형 간염 보균산모에서 출생한 신생아에서 수직감염의 기전과 예방

신필진,배종우,정사준,최용묵,김승보 한국의학사 1995 診斷과治療 Vol.15 No.1

실리카 도파로형 WDM 광 필터의 설계 및 해석에 관한 연구

최대필,문성욱,정영철,신장욱,정명영,최태구 광운대학교 신기술연구소 1997 신기술연구소논문집 Vol.26 No.-

본 논문에서는 Mach-Zehnder 간섭기로 구성된 1×4 필터와 성형결합기로 구성된 5×5 광도파로 격자 라우터를 BPM CAD TOOL을 이용하여 설계하였고 최적화 하였다. 마스크 레이아웃을 효율적으로 실현하기 위한 전체 도파구조를 설계하였다. BPM 전산모사를 통해 설계된 소자의 WDM 특성을 0.8 nm(100 ㎓)와 8.0 nm(1000 ㎓)의 채널간격에 대해서 살펴보았다. 설계된 소자는 두가지 채널간격 모두에 대하여 3.5dB 이내의 잉여손실, 0.5dB 이내의 균일성과 -22dB 이내의 누화(crosstalk)특성을 보임을 확인하였다. In this paper, we designed and optimized 1×4 WDM Filter composed of Mach-Zehnder Interferometer and 5×5 Waveguide Grating Router(WGR) composed of two star couplers. The overall guiding structure is designed to realize the mask layout efficiently. Through the BPM simulation, we demonstrated WDM characteristics of these devices with 0.8 nm and 8.0 nm channel-spacing. It is shown that the excess loss is less than 3.5dB, uniformity less than 0.5dB and the crosstalk smaller than –22 dB for both the channel spacings.

케일녹즙이 고콜레스테롤식이 흰쥐의 혈청 및 간장의 지질성분에 미치는 영향

박필숙,강진순,정승용,박재옥,김행자 慶尙大學校 1987 論文集 Vol.26 No.1

체중 60~65㎏의 wistar계 숫쥐에게 cholesterol 무첨가식이에 물과 kale 녹즙을 각각 급여하여 혈청중 총 cholesterol 농도, HDL-cholesterol 농도, 간장중의 총 cholesterol 농도, 혈청 및 간장중의 TG와 PL의 농도 그리고 과산화지질 농도를 측정하여 다음과 같은 결과를 얻었다. 1) 혈청중 총 cholesterol 농도는 cholesterol 무첨가식이군인 1,2군산 cholesterol 첨가이식군인 3,4, 5군간에서 각각 유의성이 없었다. HDL-cholesterol 농도는 cholesterol 무첨가식이군인 1,2군과cholesterol 첨가이식이군인 3,4,5군에서 kale 녹즙을 급여한 군이 유의적으로 높았다. 2)혈청의 중성지방농도는 전 군간에 유의성은 없으나 물ㅇㄹ 급여한 군보다 kale 녹즙을급여한 군에 있어 약간 낮은 경향이었다. 혈청의 인지질 농도는 cholesterol 무첨가식이군 및 cholesterol 첨가이식군 모두 물을 급여한 군보다 kale 녹즙을 급여한 군이 유의적으로 높았다. 3)간장의 총 cholesterol 농도는 cholesterol 첨가식이군에 있어 물을 급여한 군이 약간 낮은 경향이었다. 4)간장의 중성지방농도는 cholesterol 무첨가식이군에 비해 cholesterol 첨가이식군인 3,4,5군이 유의적으로 높았으며, 이들 군간에 있어서는 kale 녹즙혼액군이 비교적 낮았다. 간장 인지질농도는 cholesterol 첨가식이군인 3,4,5군간에 있어 kale 녹즙과 녹즙혼액을 급여한 3,4,군이 비교적 낮았다. 5)혈청 및 간장의 과산화지질농도는 cholesterol 첨가이식군인 3,4,5군이 무첨가식이군에 비해 대체로 낮았으며 또한 kale녹즙과 녹즙혼액을 급여한 군에 있어 유의적으로 낮았다. 이상의 결과로 보아 kale 녹즙의 섭취는 혈청 HDL-cholesterol 농도의 상승효과가 있고 간장 중 cholesterol 및 인지질의 농도를 다소나마 저하시키며 또한 혈청및 간장의 과산화지질농도의 지하작요이 있으므로서 혈청 및 간장의 지질개선효과가 있는 것으로 사료된다. The effect of kale juice, Brassica oleracea, on lpid components in serum and liver were investigated by feeding male rats of wistar for four weeks. And the concentrations of T-cholesterol,phospholipid, triglyceride and lipid peroxide in serum and liver were analysed along with the HDL-cholesterol concentration of serum . The results were as follows: 1)There was not found ny significant influence on the concentration of T-cholesterol in serum, butsome of relatively higher inluence but some of relatively higher influence on the HDL-cholesterol concentration. 2)For the group fed with 100% kale juice the concentration of triglyceride was shown lower than for any other groups, while that of phospholipid was shown higher. 3)The concentration T-cholesterol in liver was shown a little lower for the kale juice groups. 4)The concentration of triglyceride in liver was remarkably decreased in the 100% kale juice group in comparison with other groups, whiel the phospholipid concentraion was not. 5)Lipid peroxide values in serum and liver were relatively decreased in the kale juice groups compared to other group.

PC12를 이용한 신경세포 허혈모델에서 irp94 유전자의 발현

김승환,양영모,하영록,정성필,유인술,김인병 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: The ischemia responsive protein 94 kDa(irp94) gene belongs to the heat shock protein 110 family and was isolated in 1999 from rat brain by transiently induced forebrain ischemia. The PC12 cell is the pheochromocytoma cell line of rat, which is differentiated to a sympathetic neuron-like cell by the stimulation of a nerve growth factor. This study is to determine whether irp94 is expressed when an ischemia-like condition is induced by ATP depletion in cultured PC12 cells in vitro. Methods: PC12 cells were maintained as monolayer cultures in RPMI-1640 medium(Sigma) supplemented with 10% horse serum, 5% fetal bovine serum, 5 mg/ml transferrin, and 1 mg/ml insulin in a humidified 5% CO2 incubator at 37℃. The ATP depleting agent antimycin A was added at concentrations of 1, 2.5, and 5 μM to simulate ischemia, and 10 μg/ml of tunicamycin, which is expected to express heat shock protein maximally, was used as a positive control. The cells were harvested after a 60-minute incubation, and the total RNA was extracted. The reverse transcription polymerase chain reaction(RT-PCR) was performed to use 501 bp irp94 cDNA as a molecular probe, and the expression of irp94 mRNA was analyzed by northern blotting. Results: The irp94 mRNA expression was enhanced, compared to the negative control group, as the concentration of antimycin A was increased. Conclusion: This study suggests that irp94 mRNA expression is enhanced as the severity of ischemia is increased. Thus, it is possible to investigate the mechanism of ischemic neuronal injury indirectly by using this in-vitro model of neuronal ischemia.

W-3계 고도 불포화지방산의 혼합비율이 다른 유지가 지질대사에 미치는 영향

박필숙,김성희,정승용,김경숙,문순열 慶尙大學校 1988 論文集 Vol.27 No.1

혈청 콜레스테롤 및 중성지방의 농도와 밀접항 관계가 있으며 혈청 lipprotein의 대사에 영향을 미치는 w-3계의 지방산의 적정한 섭취 비율을 구명하기 위한 일환으로써 들깨기름과 라아드의 혼합비율을 달리하여 흰쥐에게 급이한 결과 다음과 같은 결과를 얻었다. 1. 혈청 총-콜레스테롤 농도는 들깨기름 7.5%+라아드2.5%를 급이한 2군에서 유의성있게 낮은 반면 10%라아드 급이군은 현저히 높았다. 2. 혈청 HDL-콜레스테롤은 3군과 4둔이 1군, 2군에 비해 낮았으며, 혈청 총-콜레스테롤에 대한 HDL-콜레스테롤 비율은 2,3,4군이 5군에 비해 높았다. 3. 혈청 중 인지질과 중성지방 농도는 5군에 비해 2,3군에서 현저하게 낮았다. 4. 간장 중 총-콜래스테롤 농도는 4군이 1,2,3군에 비해 비교적 낮았다. 5. 간장 중 인지질 농도는 각 군간에 유의적인 차이는 없었으나 중성지방의 농도는 5군보다 1,2군에서 유의성 있게 낮았다. 6. 시험유지의 지방산조성은 1군에서 lionenic acid가 dir 58%이었으며, 5군에서는 w-3계 PUFA가 0.1%, oleic acid가 47.5%이었다. 7. 혈청 lipoprotein pattern은 2군에서 HDL함량이 가장 많은 반면 LDL함량은 가장 적은 경향이었다. In order to investigate the suitable composition of the w-3 series polyunsaturated fatty acid on lipoprotein metabolism related with cholesterol and triglyceride concentrations in serum, it was examined the effects of w-3 PUFA contents of mixed fat on oil lipid metabolism in serum and liver of rats. The results were summerized as follow ; 1. The concentration of T-cholesterol in serum of rats was lowest in the group 2 (7.5% perilla oil + 2.5% lard). whereas it was highest in group 5 (10% lard). 2. The concentration of HDL-cholesterol in serum was lower in group 3, 4 than in group 1, 2 and the ratio of HDL-cholesterol to T-cholesterol were higher in group 2, 3, 4 than in group 5. 3. The concentrations of phospholipid and trigyceride in serum were significantly lower in group 2, 3 than in group 5. 4. The concentration of T-cholesterol in the liver was relatively lower in group 4 than in group 1, 2, 3. 5. Phospholipid values in the liver were not significantly different between groups, but the concentrations of triglyceride were remarkably lower in group 1, 2 than those of the group 5. 6. Linolenic acid in group 1 (10% perilla oil), in the fatty acid composition of test lipids used in the experiment. was as much as about 58%, but w-3 PUFA in group 5 was only 0.1% and oleic acid was as much as about 58%, but w-3 PUFA in group 5 was only 0.1% and oleic acid was as much as 47.5%. 7. HDL contents, in lipoprotein composition of serum were the most in group 2 (7.5% perilla oil+2.5% lard), while LDL contents were the least.

파라콰트 중독 흰쥐에서 항산화제 U-74389G 의 치료 효과

오진호,정성필,임훈,노성훈,김혜영,김승호,이한식,민진식 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: This study was conducted to verify the hypothesis that the suppression of lipid peroxidation with the antioxidant, U-74389G, could improve the survivability of paraquat intoxicated rats. Methods: First, we obtained the 24-h mortality by using several paraquat dosages and calculated the 24-h LD5O in 24 male Wistar rats(250~350 g). To examine the effect of U-74389G, we divided the rats in 4 groups: a control group and U-74389G only group, a paraquat only group, and a paraquat plus U-74389G group(n=10 each). Paraquat, 35 mg/kg, was injected intraperitoneally at 0 h. U-74389G, 10mg/kg, was administered intraperitoneally at 0, 12 h or at 1, 12 h in the respective groups. The rats were observed for 24 hours. At 24 h, plasma and lung, liver, and kidney tissues were obtained after sacrificing the surviving rats to determine the degree of lipid peroxidation by using a thiobarbituric acid reactive substances (TBARS ) quantitative analysis. Results: The 24-h LD50 of paraquat was calculated as 40 mg/kg in our rats. The 24-h mortality was as follows: control group and U-74389G group 0%, paraquat group 30%, and paraquat plus U-74389G group 10%. The TBARS analysis showed no differences between the U-74389G and the control groups. The paraquat group showed significantly increased TBARS levels in the serum and in the kidney and lung tissue compared to the control group(p<0.05). With U-74389G, the increased TBARS levels were significantly decreased in the plasma, kidney, and lung tissues compared to the paraquat group(p<0.05). However, in the liver tissue, there were no significant differences among the groups. Conclusion: A 21-aminosteroid antioxidant, U-74389G, improved the survivablity of paraquat-intoxicated rats through the suppression of lipid peroxidation. Our result suggests the possibility of clinical application of this drug as an antidote for paraquat poisoning.