Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

敎師의 職務滿足度 分析에 관한 硏究

이항재,이경복 順天鄕大學校 人文科學硏究所 1998 순천향 인문과학논총 Vol.6 No.-

사용자 분석을 통한 대학기숙사 거주환경 개선에 관한 연구

이청웅,박행자,임경희 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.2

This study, as a case study of four university dormitories in Gwangiu, examined the situation of interior space and conducted a quantitative survey invesitgating importance about the interior space and assessment factors of residential environment on users. Then user satisfaction at the assessment factors of residential environment was analyzed to draw problems and explore improvement. The results showed the following problems that should be resolved: Bedrooms should increase the area of convex space; toilets and bathrooms should be changed into a cluster type at least in common use by floor; rest space should reinforce heating and cooling system; robby space should become mixed space for various user's behavior; private fitness rooms should be planned as group fitness space; private libraries should reinforce lighting facilities: and computer rooms should complement and improve HVAC.

가시화 기법을 통한 선박 구조설계 지원 전문가 시스템 개발

이경호,한순흥,이동곤,김은기 한국경영과학회 1993 한국경영과학회 학술대회논문집 Vol.- No.1

본 논문에서는 설계 경험이 적은 설계자가 효율적으로 선급규정을 이용하여 선체설계를 할 수 있도록, 범용 전문가시스템 쉘(Shell)인 NEXPERT를 이용하여, 선체 구조설계를 지원할 수 있는 전문가 시스템을 개발하였다. 먼저, 선급 규정집(LLoyd Rule)과 기존의 rule scantling프로그램으로 부터 지식을 추출하고, 이를 바탕으로 객체지향개념에 의해 지식의 그룹핑과 계층화를 시도하였다. 또한, NEXPERT가 갖고 있는 외부 프로그램과의 접속기능(Callable Interface)을 이용하여 각종 설계에 필요한 프로그램과 실적선 데이타베이스를 접속하였다. 특히, Motif를 이용한 그래픽 사용자 인터페이스(Graphical User Interface : GUI)를 도입하였고, 설계자가 결과에 대해 검토를 할 수 있도록 Knowledge tracing 기능을 부여함으로써, 사용자 지향의 구조부재 치수 결정 지원 전문가 시스템을 개발하였다. 이러한 연구를 통해 범용 전문가 시스템이 선박설계에 이용될 수 있다는 가능성을 보였으며, 그 추구해야 할 방향을 설정하였다. 특히, 어떤 설계결정에 이르는데 관여한 규칙들을 보여주며,객체로 정의된 설계 부재들의 값들을 확인하게 함으로써 설계자가 효율적인 설계를 수행할 수 있게 되었다.

胃疾患의 食餌療法

강미경,김미숙,김선숙,김수향,양이선 효성여자대학교 가정대학 학도호국단 1987 家政大論集 Vol.6 No.-

The circumstance being surrounded with a rapidly changing society causes us to build up manystresses during our modern life. For this reason, there is increasing the occurrence of gastroentericdisorder in frequency. The dietary treatment of gastroenteric disorder requires to prepare the program necessary fora meal's amount quality and cookery with careful attention. In addition, it is essential for gastroentericpatients to fully supplement the needed nutritive substance depending on the degree of the trouble,by controling their mealtimes. Consequently, the successful dietary treatment entirely depends uponthe close cooperation between the patient involving his family and doctors and dietitians becausethe patient has to observe the standard of the programed dietary treatment. And it also is very impor-tant that the person who prepares table for these patients, attempts to improve their appetite, bysupplying foods suitable for their digestion and taste on the basis of the dietary treatment's standard. Several mental elements of gastroenteric patients have great effect upon the recovery of their di-sorder so that their family and nurses must lead these patients to stabilize their mind and to berestored to health as well as the adequate medical treatment.

非定常熱源인 方形波펄즈 加熱法에 의한 세라믹 高誘電體의 熱物性値測定에 關한 硏究

車京玉,李興周,張熙碩 明知大學校 産業技術硏究所 1993 産業技術硏究所論文集 Vol.12 No.-

본 연구에서는 비정상열원인 방형과 펄즈 가열법에 의하여 고주파 특성이 우수하고 내열성이 양호하며 산업용 전자기에 필수적인 세라믹 고유전체의 MT-2, CT, SN, PZT-20, TLT-13의 열물성치를 측정하였다. 여기서 측정의 이론을 설정하고 측정의 자동화를 시도하므로 매우 짧은 시간내 시료의 열확산계수, 비열 및 열전도율을 얻을 수 있었다. 또한 시료는 실온으로부터 약 1300K 온도 범위까지 측정하였으며 높은 유전율이 열물성에 미치는 영향에 대해서 검토 및 분석을 하였다. In this paper, thermophysical properties of the ceramic high-dielectric materials such as MT-2, CT, SL, PZT-20, TLT-13 were measured by a single rectangular pulse heating method. The values of Thermal diffusivities, specific heats, and thermal conductivities were measured as a function of temperature ranging from room temperature to 13--K. Thus, the effects of dielectricity upon thermophysical properties were analysed in detail.

치료 저항성 환청을 나타내는 만성 정신분열병 환자에서 측두두정부 반복 경두개자기자극의 효과

정경희,최충식,박진수,이규항,함웅,이승환,채정호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.5

Objectives : It has been reported that repetitive transcranial magnetic stimulation (rTMS), which stimulate the focal region of brain may relieve auditory hallucination in patient with schizophrenia. This study was aimed to evaluate the effect of rTMS on left and right temporopahetal cortex in schizophrenic patients with treatment-resistant auditory hallucination. Methods : Twenty-six patients with chronic schizophrenia with treatment -resistant auditory hallucination were randomly a1-located to rTMS group on left and right temporoparietal head regions. The rTMS was applied to TP3/4 region by EEG 10-20 international system at 1 Hz for 20 minutes per day for 101reatment days. A Figure of eight TMS coil with a solid core was used for rTMS at 100% of individual motor threshold. Efficacy was evaluated with Auditory Hallucination Rating Scale (HRS), Positive and Negative Symptoms Scale (PANSS), Clinical Global Impression (CGI) Scale on 5 and 10 days of treatment. Results : Ten days administration of l-Hz rTMS to temporopahetal cortex significant improved frequency (p<0.001) and attentional salience (p=0.038) of auditory hallucination, Positive (p<0.001) and negative symptom (p=0.02), and CGI-impro-vement (p<0.001). There were no significant differences between right and left side stimulations. Conclusion : These results suggest that the low frequency rTMS on temporopahetal cortex may be useful in treating Schizo-phrenic patients with treatment-resistant auditory hallucination and it could be a useful novel treatment option.

영아에서 발생한 골수섬유증 1례

최성민,이동석,권경배,김두권,김나연,원유흥,이준희,권영무 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

특발성 골수섬유증은 만성 골수증식성 질환의 하나로 말초혈액도말에서 백적아구증, 부동변형적혈구 눈물방울세포 소견을 보이며, 골수 생검상 망상 섬유와 교원섬유로 구성된 섬유조직이 증가하는 골수의 섬유화 소견을 보이며, 골수외 조혈작용으로 인한 대상적 비장·간장 종대를 특징으로 하는 소아에서는 극히 드문 질환이다. 저자들은 본원에 복부종괴와 안면창백으로 입원하였던 8개월된 남아에서 임상증상, 말초혈액검사, 골수생검에서 특발성 골수섬유증으로 진단되어 Methylprednisolone 대량 정주 요법으로 치료하였으나 진단7개월만에 패혈증과 심부전으로 사망했던 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Idiopathic myelofibrosis is a chronic myeloproliferative disorder characterized by anemia, leukoerythroblastosis, anisopoikilocytosis, tear-drop cell, extramedullary hematopoiesis with hepatosplenomegaly and varying degrees of myelofibrosis of bone marrow. The mean age is about 60 years, and pediatric cases are rare. We experienced a case of idiopathic myelofibrosis in a 8 months old male who was presented with pallor, huge splenomegaly, leukoerythroblastosis in peripheral blood, myelofibrosis in bone marrow biopsy. We presented a case of idiopathic myelofibrosis with brief review of the literatures.

학업우수 초등학생 자녀를 둔 어머니의 진로지도 경험에 관한 질적 연구

하정,이항심,정혜윤,김은덕,유성경 한국직업능력개발원 2007 직업능력개발연구 Vol.10 No.3

본 연구는 학업우수 초등학생 자녀를 둔 어머니의 진로지도 경험을 한국의 사회·문화적 맥락에서 밝히고자 하였다. 이를 위해 서울지역에 살고 있는 초등학교 6학년인 학업우수아의 어머니 9명을 심층면담하고, 질적 연구 방법으로 분석하였다. 연구 결과, 107개의 개념과 44개의 하위 범주, 그리고 14개의 범주가 도출되었다. 어머니들의 진로지도 경험을 포괄하는 핵심범주로는 '자녀의 성취를 위해 자녀의 특성을 존중하고 자녀의 자율성을 키우면서 자신이 바람직하다고 여기는 방향으로 이끌어감'으로 밝혀졌다. 연구 결과를 바탕으로 학업우수 초등학생 자녀를 둔 어머니의 진로지도 경험의 실체를 논의하였으며, 부모를 대상으로 한 진로교육프로그램의 필요성과 후속 연구에 대한 제언을 하였다. This study examined the experience about career guidance of mother who has a academically talented child in Korea. Nine mothers were interviewed and the interview data were analyzed based on the Qualitative Research Method. Result has107 concepts, 44 subcategories and 14 categories. The core category is "The mothers consider their children's characteristics for the children's accomplishment and foster children's self-control and lead their children to desirable object the mothers view".Implications of the results and suggestions for future research are discussed.