코디세핀이 마우스 복강 대식세포에서 전염증성 사이토카인의 생성에 미치는 영향

서민정(Min-Jeong Seo),강병원(Byoung-Won Kang),김민정(Min-Jeong Kim),이혜현(Hye-Hyeon Lee),서권일(Kwon-il Seo),김광혁(Kwang-Hyuk Kim),정영기(Yong-Kee Jeong) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.1

본 연구는 동충하초(Cordyces militaris) 유래의 기능성 물질인 코디세핀의 면역활성을 검증하기 위하여 C57BL6 마우스 복강 대식세포를 이용하여 코디세핀이 대식세포의 활성화에 미치는 영향에 대하여 시험하였다. 그 결과 LPS에 의해 유도된 마우스 복강세포는 코디세핀의 작용에 의해 IL-1β, IL-12, TNF-α의 염증성 사이토카인의 생성이 증대되어 초기 염증매개 반응을 유도하여 선천면역반응의 활성화와 그리고 면역작용에 있어 후기 적응면역의 전환으로의 T 림프구의 활성화가 예상된다. 또한 IL-6의 생성증대로 활성화된 T 림프구에 의해 B 림프구의 항체생성반응을 매개하는 면역반응도 상승할 것으로 사료된다. 그리고 대식세포에 의한 염증반응에서 염증매개인자인 NO와 H₂O₂의 생성을 증대시킴에 따라 대식세포의 독성작용을 활성화시켜 염증반응을 효과적으로 유도할 것으로 보이며, 또한 H₂O₂의 후기 생성을 저해하였는데 이는 염증반응에 유도될 수 있는 세포의 손상으로부터 세포를 보호할 수 있을 것으로 사료된다. 따라서 코디세핀은 외부인자로부터 염증매개성 면역반응의 증강작용을 나타내는 것으로 사료된다. The effect of cordycepin purified from Cordyceps militaris on macrophage activation was investigated in peritoneal macrophages isolated from C57BL6 mice. Lipopolysaccharide-induced mouse peritoneal cells showed that cordycepin treatment increased the expression of the inflammatory cytokines interleukin (IL)-1β, IL-12, and tumor necrosis factor-α (TNF-α), leading to early inflammation-mediated reactions, the activation of immunological responses, and T lymphocyte activation. T lymphocytes, activated by a greater production of IL-6, resulted in antibody-generating immune reactions, suggesting that cordycepin was effective at inducing immunological responses. Consistent with the increase in the inflammation-mediating factors including nitric oxide (NO) and hydrogen peroxide (H₂O₂), the toxic response of macrophages was activated and effectively induced inflammation. These findings demonstrate that cordycepin is involved in reducing cell injury provoked by inflammatory reactions. Therefore, these results suggest that cordycepin treatment of mouse peritoneal cells induces inflammation-mediated immunological responses and immunostimulation.

GM-CSF Promotes Antitumor Immunity by Inducing Th9 Cell Responses

Kim, Il-Kyu,Koh, Choong-Hyun,Jeon, Insu,Shin, Kwang-Soo,Kang, Tae-Seung,Bae, Eun-Ah,Seo, Hyungseok,Ko, Hyun-Ja,Kim, Byung-Seok,Chung, Yeonseok,Kang, Chang-Yuil American Association for Cancer Research 2019 Cancer immunology research Vol.7 No.3

<P>Granulocyte-macrophage colony-stimulating factor (GM-CSF) functions as an adjuvant for antitumor immunity through an unclear mechanism. By activating monocyte-derived dendritic cells, GM-CSF induces Th9 development and IL9 production, which facilitates antitumor cytotoxic T lymphocyte responses.</P><P>GM-CSF as an adjuvant has been shown to promote antitumor immunity in mice and humans; however, the underlying mechanism of GM-CSF–induced antitumor immunity remains incompletely understood. In this study, we demonstrate that GM-CSF potentiates the efficacy of cancer vaccines through IL9-producing Th (Th9) cells. GM-CSF selectively enhanced Th9 cell differentiation by regulating the COX2–PGE<SUB>2</SUB> pathway while inhibiting the differentiation of induced regulatory T (iTreg) cells <I>in vitro</I> and <I>in vivo</I>. GM-CSF–activated monocyte-derived dendritic cells converted tumor-specific nai¨ve Th cells into Th9 cells, and delayed tumor growth by inducing antitumor CTLs in an IL9-dependent manner. Our findings reveal a mechanism for the adjuvanticity of GM-CSF and provide a rationale for the use of GM-CSF in cancer vaccines.</P>

큰느타리버섯(Pleurotus eryngii) 조다당체의 면역세포 활성화 효과

강혜인(Hye-In Kang),김재용(Jae-Yong Kim),문광덕(Kwang-Deog Moon),서권일(Kwon-Il Seo),조영숙(Young-Sook Cho),이상대(Sang-Dae Lee),이성태(Sung-Tae Yee) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.7

큰느타리버섯의 기능성 식품으로서 활용도를 높이기 위해 동결 건조된 자실체에서 분리한 조다당체 추출물이 면역세포 활성에 미치는 효과를 조사한 결과는 다음과 같다. 조다당체 추출물은 300 및 1,000 ㎍/mL 농도에서 비장세포의 증식을 유도하였으며, 이때 비장세포는 IL-6와 IFN-γ 분비를 유도하는 것으로 나타났다. 조다당체 추출물은 농도 의존적으로 B세포의 증식을 유도하였으며, 특히 100 ㎍/mL 농도 이상에서는 B세포의 증식이 현저히 증가하는 것으로 나타났다. 그리고 조다당체 추출물 1,000 ㎍/mL 농도에서 B세포가 생산하는 IgG1, IgG2a, IgG3의 분비량이 현저히 증가하였다. 또한 농도 의존적으로 대식세포주의 일산화질소 생산을 유도하였으며, 대식세포가 분비하는 IL-6, TNF-α, GM-CSF의 생산도 현저히 증가하는 것을 확인할 수 있었다. The objective of the current study was to determine the effects of the crude polysaccharide isolated from fruit body of Pleurotus eryngii on mouse splenocytes, B cells, and macrophages in vitro. The crude poly-saccharides directly induced the proliferation of spleen cells in a dose-dependent manner and increased IL-6 and IFN-γ synthesis. The crude polysaccharides also increased the proliferation of B cells in a dose-dependent manner. The production of immunoglobulin G1, G2a and IgG3 in the presence of the crude polysaccharides was increased progressively in the culture supernatant. When the crude polysaccharide were used in macrophage cell line (RAW264.7) stimulation, there were marked induction of NO synthesis in a dose-dependent manner and IL-6, TNF-γ and GM-CSF synthesis. These results suggest that the crude polysaccharide isolated from fruit body of Pleurotus eryngii seem to act as a potent immunomodulator causing augmentation of immune cell activity, and thus could be used as a biological response modifier having possible therapeutic effects against immunological disorders, without any side effects.

교원효소로 유발된 랫드 대뇌 출혈병변의 병리학적 관찰

서일복,소경순,김광호,김영석 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

This study was carried out to characterized the hemorrhagic lesion sof the collagenase induced hemorrhagic stroke in rats. Fifty three Sprague-Dawley male rats were divided into two group. Group Ⅰ was served as model animals for hemorrhagic stroke, which were infused with 2㎕ saline containing 0.5unit collagenase(type Ⅶ) into the caudaate nuclei. Group Ⅱ was served as control animals, which were infused with 2㎕ saline only into the caudate nuclei. Six heaks of the rats of group Ⅰ were sacrificed at 30 min, 1, 5, 24 hours, 2, 5, 10 days after the infusion , and 3 heads of rats were sacrificed at 15 days after the infusion. Two heads of the rats of group Ⅱ were sacrificed at 30min, 1,5, 24 hours after the infusion. The brains of the rats were removed and examined grossly and histopathologically. Grossly, pin point sized or linear hemorrhage were occurred at 30minutes and the hemorrhagic lesions extended to 5mm in diameter at 24 hours. After then the hemorrhagic lesions faded gradually. Histopathologically, extended hemorrhage with degeneration of brain parenchyma were observed at 5 hours. Large area of malacia were observed at 24 hours. At 5 days, proliferation of astrocytes, proliferation of newly formed capillaries, and infiltration of lipid laden macrophages were seen at the periphery of the area of malacia. At 15 days, most of the area of malacia were replaced by granulation tissue. These observations may be useful parameters for the screening of therapeutic drugs using collagenase induced hemorrhagic stroke animal model.

Pyrantel Pamoate (Combantrin)에 依한 蛔蟲 및 鉤蟲感染의 集團治療

徐丙卨,朴定圭,趙昇烈,姜信榮,魚光本,康世喆,李源宰,李正雨,黃邱一 中央醫學社 1972 中央醫學 Vol.23 No.6

The mass treatment trial of pyrantel pamoate on Ascaris lumbricoides and Ancylostoma duodenale infections was carried out to school-children and inhabitants in rural areas in the central part of Korea. Pyrantel pamoate ("Combantrin") tablet (123 mg base) was administered as a single dose of 10. 0 mg per kg. body weight to a total of 4,198 Ascaris infected cases selected out of 4,436 (41. 6%) positives, who were screened from the total 10,660 collected stool specimens. Of these, only 3,169. cases were followed up after treatment to provide an analysis in this study. Incidentally 161 cases with concomittently infected hookworm were also subjected to assess the efficacy of pyrantel pamoate against A. duodenale. The egg negative conversion rate on A. lumbricoides was 95.3%, and on A. duodenale, 97.5% four weeks following treatment. In failed cases, the egg reduction rates on both of the infections were 97.6% and 97.3% respectively. No side effects and no problem in drug administration were demonstrated. It is concluded that this study confirms the high efficacy of pyrantel pamoate in the treatment of Ascaris lumbricoides and Ancylostoma duodenale infections and also proves the acceptability and safety of this drug in the mass treatment of ascariasis in a population with relatively high worm burden.

김치 발효중 생체활성 아민 생성에 관한 연구

서일원,류춘선,이주돈,이영환,변광의 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Biogenic amines can be formed and degraded either as the result of endogenous amino acid decarboxylase activity in raw materials or by the growth of decarboxylase-positive microorgannisms under conditions favorable to enzyme activity. Three biogenic amines(Tryptamine, Histamine, Tyramine) were intensively investigated by the HPLC during Kimchi fermentation at 5℃. The patterns of microfloral changes in lactic acid bacterial group, Leuconostoc, Streptococcus and Lactobacillus, were similar to the typical low-temperature fermentation. The population of microbial growth were high after 10 days and gradually decreased. The levels of Tyramine were lowered(10mg/kg) during this mid-periods(8∼16 days) but very high in initial-(15mg/kg) and final-periods (30mg/kg), in which the total acidity ranged between 0.2∼0.35% and 0.4∼0.6%, while the acidity in mid-periods revealed 0.35∼0.4% beginning the initial ripening period. Leuconostoc was highly increased in number in mid-period but slightly declined as the acidity increased, whereas Streptococcus was highly distributed in initial-period and Lactobacillus in final-period. The levels of Tryptamine and Histamine were relatively low throughout the whole fermentation period, but the profile of Tryptamine level was very similar to the case of Tyramine. Histamine was maintained at a certain level after mid-period of fermentation.

LC/MS/MS 분석법을 이용한 Amlodipine의 약물동태연구

서정원,윤민혁,강원구,권광일 충남대학교 약학대학 의약품개발연구소 2007 藥學論文集 Vol.22 No.-

The aim of this study were to confirm the analysis method and also to estimate the pharmacokinetic parameters of amlodipine in human volunteers. In an open-label single-dose pharmacokinetic study, a group, consisting of 24 healthy volunteers, received single oral dose of 5mg amlodipine. Blood sample were taken for up to 120 hours. The concentration of amlodipine in these body fluids was determinated using a validated high-performance liquid chromatography(HPLC) method with tandem mass spectrometry. Amlodipine and ketoconazole, an internal standard, were extracted from plasma using ethyl acetate in the presence of 0.1M sodium carbonate. After drying the organic layer, the residue was reconstituted in mobile phase(acetonitrile : water = 70 : 30 v/v (0.1% formic acid)) and injected onto a Zorvax C18 column (2.1 × 100 mm, 3.5 ㎛ particles). The isocratic mobile phase was eluted at 0.2ml/min. The ion transitions monitored in multiple reaction-monitoring mode were m/z 410.10 → 294.95 and 532.11 → 81.95, respectively. The coefficient of variance of the assay precision was less than 12%, and the accuracy exceeded 99.1%. The limit of quantification was 0.1 ng/ml. The pharmacokinetic parameters were calculated with non-compartmental(AUC, C_(max), T_(max), CL_(t), V/F) and compartmental(K_(el), K_(a), t_(lag)) pharmacokinetic analysis using WinNonlin program. The estimated means of AUC_(0-120hr), C_(max) and T_(max) were 196.90 ± 5.02 ng·hr/ml, 3.36 ± 0.09 ng/ml and 10.44 ± 0.61 hr, respectively. The means of other pharmacokinetic parameters(V/F, CL_(t), K_(el), K_(a) and t_(lag)) were 1208.06 ± 50.61 L, 25.39 ± 0.65 L/hr, 0.2806 ± 0.0294 hr^(-1), 0.0210 ± 0.0008 hr-1 and 0.4574 ± 0.0635 hours, respectively.

식이 상태와 카페인 투여가 일회적 운동 시 흰쥐 가자미근의 제 4형 당수송체에 미치는 영향

김광찬,김종오,오명진,윤재석,서태범,윤진환,정일규 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to evaluate the effects of diet condition and caffeine ingestion prior to long distance treadmill running on the GLUT-4 protein expression in Sprague-Dawley rats' soleus muscle. Total is rats were used For subjects and assigned to six groups: Fasting control(FaC group), feeding control(FeC), asting+exercise(FaE), feeding +exercise(FeE), Fasting +caffeine+exercise(FaCE), and Feeding+caffeine+exercise(FeCE). Each animal belongs to fasting group was forced to be in fasting state at least for 14 hours, The food was provided to each rat in feeding group 12minutes prior to treadmill running and the caffeine was ingested through the stainless steel tube 60minutes prior to exercise. All rats in exercise groups were forced to perform treadmill running for B9 minutes at the speed of 18m/min, and 0%grade, immediately after exercise, all animal was anesthetized by Chlorofolm (DC Chemical, Co, Ltd) and about 10ml of blood was drawn from each animal's heart and than soleus muscle was exposed and separated from the rat's leg. we found that the GLUT-4 in feeding groups increased much significantly compared to fasting groups(p<.05). The exercise in fasting state appeared to suppress the expression of GLUT-4 protein different from the exercise in feeding state. Cafreine ingestion prior to treadmill running of 60 minutes induced the significant increase or GLUT-4 protein expression both in fasting or feeding states.

Afloqualone의 LC/MS/MS분석과 건강한 성인 지원자에 대한 약물동태 연구

이서판,이병요,윤휘열,이은주,권광일 충남대학교 약학대학 의약품개발연구소 2008 藥學論文集 Vol.23 No.-

Afloqualone is a centrally acting muscle relaxant that inhibits mono- and polysynaptic reflexes. The purpose of this study was to estimate the pharmacokinetic parameters of afloqualone in healthy volunteers. The pharmacokinetics of afloqualone tablet was examined on 24 healthy volunteers who received a single oral dose(20 Bg) of each preparation in the fasting state. Blood samples were ten at 0, 0.5, 1, 1.5 2 3 4 6 8 10, 12 and 24 hr after drug administration. Blood concentrations of afloqualone were determined using a liquid chromatography tandem mass spectrometry(LC/MS/MS) systems. A two compartment model was used to explain the pharmacokinetic properties of afloqualone. The pharmacokinetic parameters were calculated with model independent(AUC, C_max, T_max, CL_t, V/F) and model dependent(K_ei, K_a, K_cp, K_pc, t_1/2) Pharmacokinetic analysis using WinNonlin program. The estimated means of AUC_0-24hours, C_max, T_max CL_t and V/F were 148.99 ± 127.39 ng·hi/ml 35.11 ± 56.62 ng/ml, 1.81 士 1.34 hr, 189.01 士 79.67 L/hr and 848.61 ± 567.06 L, respectively. The model dependent parameters(K_ei, K_a, K_cp, K_pc, t_1/2) were 0.14 ± 0.18 hr^(-1), 11.64 ± 30.03 hr^(-1), 0.34 ± 0.39 hr^(-1), 0.1 ± 0.16 hr^(-1) and 10.56 ± 4.30 hr, respectively. In conclusion a two compartment model was best described the pharmacokinetic behavior of afloqualone in healthy human.

3차원 영상 재생을 위한 집적결상법에서 기본영상 획득 방법과 기본렌즈의 형태에 따른 기본영상의 특성 연구

서장일,강근호,서광범,차성도,신승호 강원대학교 정보통신연구소 2006 정보통신논문지 Vol.10 No.-

We have investigated the properties of an elemental image of singlet elemental lens for film and direct pick-up method, respectively. The optimized conditions, which are minimizing the distortions of the elemental image, of the elemental lens have been analyzed with the geometrical method and the comparison of the picked up images. As a result, it is down that the elemental image quality is strongly depend on not only the lens shape but also the pick-up method.