Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

지리산국립공원 심원계곡 생태경관 복원공사지역 식생 변화

정태준 ( Tae-jun Jung ),김연경 ( Yeon-Gyeong Kim ),김영진 ( Young-jin Kim ),정명희 ( Myung-hee Jung ),박경희 ( Kyoung-hee Park ),신창근 ( Chang-keun Shin ),박선홍 ( Seung-hong Park ),김영선 ( Young-sun Kim ) 한국환경생태학회 2021 한국환경생태학회지 Vol.35 No.3

본 연구는 지리산국립공원 심원계곡 생태경관 복원공사지역의 모니터링 결과를 분석하여 체계적인 복원관리를 도모할 수 있는 기초자료를 확보하는데 그 목적이 있다. 복원사업이 완료된 2017년도에 식생 모니터링을 위한 조사구 12개소와 대조구 4개소를 설치하였고, 2017년과 2020년의 상대우점치, 종다양도지수, 유사도 등의 변화를 분석하였다. 복원사업 시 수목이 식재되었던 조사구는 종다양도지수가 0∼1.4552로 나타났고, 대조구와의 유사도지수에서는 1개 조사구가 1.32%로 분석된 것을 제외하고 모두 0%로 나타났다. 조사구의 종다양도지수 및 유사도지수가 매우 낮은 것은 복원사업 시 식재된 수목이 고사하거나 멧돼지 피해, 유수에 의한 침식으로 유실되는 등의 영향으로 판단되었다. 반면 복원사업 시 수목이 식재되지 않은 미식재 조사구는 종다양도지수가 0.9538∼2.3222로 나타났고, 유사도지수는 최대 8.33%까지 분석되었다. 국립공원 생태경관 복원공사지역의 복원 공법 개발을 위한 장기 모니터링과, 미식재 조사구의 천이과정을 분석하는 지속적인 연구가 필요하다. This study aims to obtain basic data for systematic restoration by analyzing the monitoring results of the Shimwon Valley Ecological Landscape Restoration Project area in Jirisan National Park. In 2017, when the restoration project was completed, 12 monitoring plots and 4 control plots were installed for vegetation monitoring, and changes in the relative dominance, species diversity index and similarity between 2017 and 2020 were analyzed. The species diversity index of the surveyed areas where trees were planted during the restoration project was 0-1.4552, and the similarity index with the control group was 0% except for one survey area at 1.32%. The very low species diversity index and similarity index in the survey areas were attributed to the loss of trees planted during the restoration project due to death, damage by wild boars, or erosion by running water. On the other hand, the species diversity index was 0.9538-2.3222 in the monitoring plot where no tree was planted, and the similarity index was analyzed to be as high as 8.33%. It is necessary to continue the long-term monitoring for the development of ecological landscape restoration methods in the national park and analysis of the succession in monitoring plots where no trees were planted.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

당 첨가량에 따른 송이정과의 물성 및 관능 특성

박미란 ( Mi Lan Park ),최수근 ( Soo Keun Choi ),정인창 ( In Chang Jung ),변광인 ( Gwang In Byun ) 한국식생활문화학회 2006 韓國食生活文化學會誌 Vol.21 No.6

農業構造變化와 農業金融問題의 再認識

朴正根,崔圭晧 全北大學校 1978 論文集 Vol.20 No.-

This study presents crucial features of structural changes in agriculture and their applications to farm finance problems. The rapid growth of economy since the 1960's in Korea, has accelerated the pace of industrialization and urbanization. Its influences have caused agricultural transformation. The process of structural transformation has been based on the interrelationships between agriculture and industry through the market for capital and labor. The rapid economic development has stimulated a great number of rural people to migrate to large cities, so the number of agricultural workers has begun to decline since 1968. Therefore, in the past, labor was the most important input of agricultural production. Nowadays, because of the shortage of labor, observations concerning the relative changes of resource inputs in agricultural production indicate that capital acts as a major factor in the substitution for labor in the expansion of agricultural output. At the same time, wide-scale substitution of capital for labor has come about because of the technological progress based on labor-saving and capital-augmentation. On the other hand, as economic development continues to increase nation's per capita income, the commodities with higher income-elasticity, which are produced mainly for market by specialized producers have also increased rapidly. Thus, increased technological progress and marketing functions have contributed to structural change, namely, the farmers purchase more of their inputs which, in turn, encourage the changes in the financial structure of the farm sector in Korea. In order to adapt to the rapid structural changes farmers are required to have a larger amounts of capital than they have had in the past, so their liquidity position is increasing. The rapidity with which these developments emerged has created problems with some credit agencies financing farmers. However, it has been generally assumed that in subsistence agriculture rural people are too poor to save, and farm finance problems have received relatively little attention. But now, on the demand side of capital, modernizing agriculture requires large infusions of credit to finance the use of purchased inputs. On the supply side of capital, the experimental data developed in Korea recently showed that rural households have save a significant part of their incomes even though per capita incomes were quite low. This situation indicates that in farm finance Korean agriculture has arrived at the threshold of a new era. The changes in the financial structure of agriculture necessitate us to examine some of the accompanying problems and implications. From the individual farmer's standpoint farm financial management will become more important and will consume a larger proportion of the operator's time. Credit agencies will meet more nearly the challenges of greatly increased credit needs, and must perform the function of transferring savings between sectors, between regions and between income classes. To insure economic progress and an equitable distribution of its profits, policy makers should prepare to create and nurture a strong appropriate institutional program.

육상 운동 지식이 초등학생의 정의적 특성에 미치는 영향 : Focused on a long distance race in physical education for 6 grade students in elementary school

박근호,전중기 대구대학교 인문과학 예술문화연구소 2003 人文科學硏究 Vol.25 No.-

The purpose of this paper is to make a comparative study of educational effects that teachers' knowledge obtained by studying text has on physical education. This will analyze how a teacher with special knowledge from text reaches study aim in physical education, and influences students who intend to accomplish study subject. The comparative study was conducted between two types of teachers. One accumulates propositional knowledge and practical knowledge of physical education according to study aim, the other does according to their experience. The focus of this study is a long run race, an athletic sport in physical education for 6 grade students in elementary school. The study result is dealt by SPSS(7.52) and shows as below. First, the teacher who instructed students after studying text developed psychomotor domain, The experimental group consists of people who study text and instruct athlete sports. While the control group depends on its experience. The research result shows an educational effect of experimental group is more significant than the control group, regardless of sex. Second, the teacher who instructed after studying text changed affective domain positively. Third, teacher's professionalism gained through studying text made higher effects on psychomotor domain than affective domain. Forth, increasing interrelation among subordinate factors of psychomotor domain was show in the experimental group, compared to the control group

輸入開放에 對應한 中長期 全北地域 農業政策方向에 關한 硏究

朴正根,徐東均 全北大學校 1992 論文集 Vol.34 No.-

This paper attempts to analyze the direction of agricultural polices of Chonbuk region in the trade liberalization and to suggest an alternative regional policy of agricultural development in Chonbuk province. The increasing pressure of agricultural import liberalization from abroad has given great burden to Chonbuk agricultural sector which has lagged behind the average growth of national agriculture. The charactersitics of Chonbuk agriculture can be summarized as wide-spread small scale farming, greater portion of old-aged labor and rice monoculture system. However, the agricultural production sector of Chonbuk becomes more and more commercialized and the consumption patterns of agricultural products become diversified and high-quality oriented continuously. The present situation of Chonbuk agricultural is in a vulnerable state to those changes. Divergent measures in Chonbuk agriculture are urgently requested for the improvement of present production structure. Among these, the increase of Chonbuk local government investment for various in infra-structure of agricultural facilitis should be carried out in such a way that agricultural productivies can be enhansed. Furthermore, it is also important to emphasize on fostering specific activities that develops an appropriate technologies induced by changes of resource endowments.

PelB Signal Sequence로 유도된 제조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

박세철,남정현,김정근,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

전사개시 단계중에 삽입되는 N말단의 methionine이 제거되고 pelB signal sequence를 삽입하여 인체 epidermal growth factor (hEGF)가 직접 분비발현 되도록 고안된 T7과 tac promoter에 의하여 각각 발현되는 pYHB101과 pYHB2를 제조하였다. 또한 T7 promoter에 의하여 발현되지만 signal sequence를 삽입하지 않은 pYHB1도 제조하여 각각 제조된 재조합 plasmid들의 발현 및 분비효율을 비교하였다. pelB signal sequence hEGF 유전자를 갖도록 제조된 pYHB101을 포함하고 있는 균주가 pYHB1보다 균체 성장속도는 낮았지만 pYHB101을 1 mM IPTG로 5시간 induction 하였을 때 13 mg/l의 rhEGF가 분비발현되어 T7 promoter의 발현 체계를 갖는 경우가 tac promoter보다 높은 발현 효율을 보였다. 이때 pYHB101에서 발현되는 rhEGF의 발현량은 준최적 조건에서 14 mg/l이었다. We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

新農政과 農業勞動力問題

朴正根 韓國農業政策學會 1993 농업경영정책연구 Vol.20 No.2

최종사용자중심의 DSS실행 성공요인에 관한 연구

박정근 京畿大學校 韓國産業經濟硏究所 1994 産業硏究 Vol.5 No.-