새로운 Acetylcholinesterase inhibitors의 합성

최순규,김형민,조승환,최학기,박유미,이용균,정대일,김인식,한정태 동아대학교 부설 기초과학연구소 2004 基礎科學硏究論文集 Vol.21 No.1

노인성 치매의 일종인 alzheimer's disease의 효과적인 억제제를 합성하기 위해 우리는 분자 모델링에 의한 가장 적합한 물질인 m-[(N,N,N-trimethylammonio)phenyl]boronic acid를 합성하였다. 출발물질인 3-aminophenylboronic acid monohydrate의 경우 boronic acid의 작용기인 hydroxyl group을 protecting시킴으로써 반응의 안정성을 기하였다. Quarternary ammonium salt는 과량의 methyliodide와 염기 촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시켜 상당히 높은 수율을 얻을 수 있었다. 또한 과량의 methyliodide와 염기촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시킨 결과 boronic acid의 protection없이도 안정하게 반응이 진행되어짐을 확인할 수 있었다. In order to syntheisize a effective inhibotor for alzheimer's disease, we synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 which is designed by molecular modeling form. We protected the hydroxyl group of 3-aminophenylboronic acid monohydrate 1 with ethlyne glycol to remove the reactivity if hydroxyl group. To synthesize m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4, we reacted 3-aminophenylboronic acid monohydrate 1 with ezcess methyl iodide and potassium hydrogen carbonate as a base-catalyst in methanol. but On executed reaction without protection on hydroxyl group, we found out the result that the hydroxyl group of boronic acid group at 3-aminophenylboronic acid monohydrate 1 didn't react with excess methyliodide. Synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 is in progress about biological tests as a plausible acetylcholinesterase inhibitor.

비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

PCR과 SDS-PAGE를 이용한 젖산균의 분류

최동훈,이중림,민승기,박종환,김해영,조재선 慶熙大學校 1998 論文集 Vol.27 No.-

Strain type of lactic acid bacteria was analysed by PCR and SDS-PAGE. PCR allows the rapid and specific detection of lactic acid bacteria using primers based on 16S rRNA gene-sequences. The analysis of protein pattern on SDS-PAGE can be used to determine the differences among the same or similar species of lactic acid bacteria. Therefore, the complementary use of PCR and SDS-PAGE method is one of simple way to classify the lactic acid bacteria and to compare with genetic analysis.

SS41 용접재의 피로크랙 진전거동에 관한 연구

최병기,권택용,정환규 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

SS41 is welded automatically and is investigated some effects of the welding residual stress in the growth and propagation of fatigue crack, so as to study the fatigue behaviour in the welding residual stress field. The summarized results are as follows; 1) In case of the load range is constant, as the stress ratio is changing to 0.1, 0.33 and 0.5 the propagation life is constant but the growth life decreases. And than, when maximum load or minimum load is constant, as the stress ratio increases the growth life and propagation life. 2) It was shown that the fatigue crack propagation ratio da/dN was almost constant regardless of the stress ratio change at constant load amplitude and that the larger stress ratio, the slower was the fatigue crack propagation ratio. 3) The opening ratio U is influenced by Kmax but it isn't only the function of Kmax because data range is very large. 4) The growing and propagation fatigue crack in the welding residual stress field is similar to original specimen after passing the original residual stress field.

환경친화성 계면활성제를 이용한 어병균의 살균효과

최상원,정관용,오남희,김은영,방정환,김정우,여문환 한국환경과학회 2000 한국환경과학회지 Vol.9 No.5

The antibacterial effect amino acid-copper(Ⅱ) surfactant on fish pathogens was studied. Fish pathogens of Edwardsiella tarda, Vibrio anguillarum, Aeromonas hydrophila and Streptococcus sp. were selected, cultured in nutrient agar and adjusted at 2 × 10 exp (5)∼10 exp (6) CFU/㎖ in phosphate buffer saline before the addtion of amino acid-copper(Ⅱ) surfactant with different concentrations. All tested pathogens died within 1 hour with 1 ppm of amino acid-copper(Ⅱ) surfactant. In comparison with formalin and ET, amino acid-copper(Ⅱ) surfactnat was more effective in antibacterial capacity.

FCL(5-FU, Carboplatin, Leucovorin) 항암 화학요법에서 Gm-CSF의 효과

최지영,김현수,김종숙,박상준,윤환중,조덕연,남상륜,김삼용 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background: One of the major side effects of cancer chemotherapy is myelosuppression. Neutropenia and/or thrombocytopenia are dose-limiting factors in chemotherapy. Colony-stimulating factor induces proliferation and functional maturation of hematopoietic progenitor cells. GM-CSF is primarily active on progenitor cells of granulocytic and monocytic lineage. Methods: Fifteen patients with histologically proven malignancy diagnosed at Chungnam National University Hospital from January 1993 to August 1995 were included in this study. We could evaluate the clinical efficacy of GM-CSF in 13 patients undergoing FCL(5-FU, Carboplatin and Leucovorin) chemotherapy; the first cycles involved no GM-CSF while the second cycles involved GM-CSF on day 6 through 15 of chemotherapy. Results: 1) The subjects were fifteen patients in all, there were five patients with head and neck cancer, which was the most common types of maligancy. There were four patients with colon cancer, two patients with stomach cancer, and one patient with breast cancer, gallbladder cancer, cervix cancer and cholangiocarcinoma respectively, two patients, who did not complete two cycles of chemotherapy were excluded. 2) Age distribution was from 38 years to 78 years with a median age of 57. 3) In FCL chemotherapy cycles with GM-CSF, the duration of neutropenia(<500/μL) was 0.5±0.3 day, while FCL chemotherapy cycles without GM-CSF, it was 2.9±0.7 day(P=0.008). 3) There was no significant difference in platelet count between the two chemotherapy cycles(P=0.133). 4) Febrile duration without GM-CSF was 4.9±2.1 day, but with GM-CSF the duration was 1.3±0.7 day, which was significantly different(P=0.003). The duration of antibiotics use with GM-CSF was 1.7±1.2 day and without GM-CSF was 6.8±3.2 day, also significantly different(P=0.002). But hospital stay between the two cycles were not significantly different(P=0.064). Conclusion: GM-CSF was effective in preventing or restoring bone marrow depression after FCL chemotherapy.

한국 연안 퇴적물에서 ^137Cs, ^90Sr 및 Pu 동위원소의 분포

최석원,진현국,김철수,노정환,김창규,노병환 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.2

한국 주변 연안 15개 정점의 퇴적물(0∼20 cm)에서 ^137Cs, ^90Sr, ^239+240Pu 의 농도와 농도비를 조사했다. ^137Cs, ^90Sr, ^238Pu 및^239+240Pu의 평균 방사능 농도는 각각 2.24±0.79 Bq·kg^-1-dry, 0.20±0.04 Bq·kg^-1-dry, 0.009±0.005 Bq·kg^-1-dry 및 0.27±0.17 Bq·kg^-1-dry이다. ^137Cs/^90Sr, ^239+240Pu/^137Cs 및 ^238Pu/229+240Pu의 평균 농도 비와 ^239Pu/240의 평균 원자 비는 각각 11.2±2.9, 0.123±0.053, 0.033±0.01 및 0.218±0.036이다. 퇴적물에서 핵종의 농도 범위와 핵종간의 농도 비는 그 동안 주변국가의 퇴적물에서 조사한 값과 비슷하였다. 퇴적물에서 ^137Cs과 ^239+240Pu의 상관계수는 0.80이다. ^137Cs과 SOM, ^239+240Pu과 점토 함량과의 상관계수는 각각 0.69, 0.67 이다. The concentrations and activity ratios of ^137Cs, ^90Sr, ^238Pu and ^239+240Pu in sediment(0∼20 cm) at 15 coastal stations of Korea were determined. The mean concentrations of ^137Cs, ^90Sr, ^238Pu and ^239+240Pu are 2.24±0.79 Bq·kg^-1-dry, 0.20±0.04 Bq·kg^-1-dry, 0.009±0.005 Bq·kg^-1-dry and 0.27±0.17 Bq·kg^-1-dry, respectively. The mean activity ratios of ^137Cs/^90Sr, ^239+240Pu/^137Cs and ^238Pu/^229+240Pu and atomic ratio of ^240Pu/^239Pu are 11.2±2.9, 0.123±0.053 and 0.033±0.017 and 0.218±0.036, respectively. The concentrations and activity ratios in sediment samples are similar to those reported from neighbouring country in the northern hemisphere. The correlation coefficient of ^137Cs and ^239+240Pu is 0.80. The correlation coefficient of ^137Cs and soil organic matter(SOM), and ^239+240Pu and clay content are 0.69 and 0.67, respectively.

광고비주얼의 전략적 선택방법 연구 : 지면광고의 언어지향적 아이디어발상 적용사례를 중심으로 focusing on examples applied for the way of creative idea that is based on language of print advertisements

최정환,양정선 제주한라대학 1999 論文集 Vol.23 No.-

The purpose of this study is to present the considerable basis when a proffer advertising visual idea system is conceived and a visual element is selected. Thus, this study is seeking for the solution that the Bite system in conceiving idea based on language applies to the practical use of the cosmetic advertisement. This is the result for the step of the creative idea. The first step of the idea is to collect materials such as the state of product, consumer, market and economics. The second idea is to represent the concept of advertising, and to conceive a proffer idea using the Bite system 1 for the materials analyzed. The third idea, it is to apply the Bite system 2 for the optional creativity, UㆍSㆍP, positioning and brand image strategy, and the Bite system 3 for extracting from the materials analyzed. Next is to apply the Bite system 4 for the transformation of word or sentence and the development step of the messages extracted. The final idea is to verify idea selecting the right sentence for the creative strategy which is decided already, and to associate the visual element in the selected sentence. The meaning of this study is to present the verification step to verify the creative idea by a designer himself or herself in the process of the creative idea. Actually, many designers come into conflict with AE(Account Executive) frequently in the process of persuasion emphasizing on the visual part of design without a logical basis. To be sure, the result of this study is not the perfect solution to systematize the creative idea. However, I am convinced that it is able to be a foundation for the practical use of the material basis beside advertising strategy for the designers. Also I hope that this study can help designers to create idea effectively.

이진 배열 기법을 이용한 내포된 곡선 성분 검출에 관한 연구

최종주,위승정,양환석 서강정보대학 1997 論文集 Vol.16 No.-

The general Hough transform was not influenced to detect the external circle and ellipse for when the circle and ellipse was detected by the external noises, it was filtered in various way, and the rest image had inclused trouble in detecting, for it was regarded as the intinernal noises. So, this paper suggests the new algoritsm to detect another circle existing inside and to remove not from the general method but from the original image when the external circle detected is excluded.

양이장단자극을 이용한 각성유지 및 수면유도

정환목,배기성,김형식,우창진,최평,이만기 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1