Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

지능형 자동차 주변 감시 시스템 설계 방안

장시웅(Si-woong Jang),박영식(Young-sik Park),성현용(Hyun-yong Sung),곽길봉(Gil-bong Kwak),김선남(Seon-nam Kim),김경진(Kyeoung-jin Kim) 한국정보기술학회 2009 Proceedings of KIIT Conference Vol.2009 No.-

본 연구는 차량 주변을 지능적으로 감시하는 자동차 주변 감시시스템에 관한 것으로, 자동차의 주행 및 주차 시 속도의 변화나 운전자의 요청에 의해 자동차 주변 지역에 관한 시야를 상황에 따라 달리하여 모니터링 함으로써 운전자가 안전한 주행 및 주차를 수행할 수 있게 하기 위한 것이다. 그러한 목적을 달성하기 위하여 본 연구에서는 차량의 주변부를 감시하기 위하여 차량의 외곽에 설치된 카메라의 각도가 지면으로부터 차량의 상부를 향하는 방향으로 변경될 수 있도록 제어하는 제어부, 카메라의 영상을 입력받아 차량주변의 영상을 생성하는 영상처리모듈, 영상처리모듈에서 생성된 영상을 출력하는 디스플레이를 포함하는 것을 특징으로 하는 지능형 자동차 주변 감시시스템을 제안한다. This study is on a vehicle surroundings monitoring system, which enables a driver to travel and park safely by monitoring vehicle surroundings, depending on the speed of vehicle and driver's control when traveling and parking. To achieve the aforementioned purpose, we propose an intelligent vehicle surroundings monitoring system, which consists of a control part, an image processing module and display module. Hence, the control module controls angle of camera to turn upward from the bottom to monitor vehicle surroundings. The image processing module merges the received images from cameras with the original vehicle image to display the vehicle’s surroundings in real time as if looking down from the sky.

미만성 축색 손상 환자의 MRI 소견 : 수상 초기 신경학적 상태 및 예후와의 관계 Correlation with Initial Neurological State and Outcome

박철웅,김은상,김재형,박인성,정진명,한종우 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.5

The authors have evaluated retrospectively the distribution and extent of traumatic lesions in brain MRI of 20 diffuse axonal injury(DAI) patients. DAI most commonly involves the white matter of the frontal lobe and posterior half of the corpus callosum. The lesions on MRI were classified according to the modified Adams staging as fellows. Stage 0 ; no lesion identified(3 cases, 15%) ; Stage 1 ; lesions confined to the lobar white matter(4 cases 20%) ; Stage 2 : lesions in the lobar white matter and corpus callosum(9 cases 45%) ; Stage 3 ; additional lesions in midbrain and upper pons(4 cases 20%). Correlations between the modified Adams staging and initial posttraumatic consciousness state, presence of focal neurological deficit and the outcome of the patients were observed. Also, the presence of corpus callosum lesions tends to be associated with subarachnoid and/or intraventricular hemorrhage. T2 weighted image was found to be the most sensitive sequence for detection of DAI lesions.

필기체 숫자인식의 최적 신경망 구조 설계를 위한 개선된 유전자 알고리즘

박찬란,김향태,정재영,위승정,최봉한,최호진,이웅기 조선대학교 통계연구소 1999 統計硏究所論文誌 Vol.1 No.1

본 논문에서는 필기체 숫자인식의 최적 신경망 구조 설계를 위하여 유전자 알고리즘과 역전파 알고리즘을 병합하는 방법을 이용하였으며, 유전자 알고리즘은 유전인자의 역할에 따라 독립적으로 적응하는 개선된 선택, 교배, 돌연변이 연산자를 제안하였다. 실험결과는 유전인자의 역할에 적응하는 개선된 연산자들의 실행으로 최적의 신경망 구조를 설계하였고, 설계된 신경망 구조에서의 학습이 가장 좋은 성능을 나타내었다. In this study, the method of using both genetic algorithm and back propagation is proposed to design the structure of optimal neural network recognizing handwritten digits. Improved selection, crossover and mutation operator which can control the genetic algorithm independently according to the role of gene are used in the proposed method. Simulation result shows that the neural network of this method can search effectively the optimal hidden node numbers, connection weight, learning rate and the momentum because of above proposed operators.

전기장 인가에 따른 DNA 신장에 관한 AFM 연구

박진영,한성웅,손세영,송성훈,김종민,Ohtani, Toshio,Muramatsu, Hiroshi,장상목 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.1

본 연구에서는 마이카 표면상에서 전기장에 의한 극히 낮은 농도의 DNA 신장에 대한 효율적인 방법론을 제시하였다. 전기장에 의한 DNA 신장을 AFM형상으로 분석한 결과, DNA 농도범위가 약 57×10 exp(-3)ng/mL-57×10 exp(-6)ng/mL일 때 매우 잘 적용되며, 신장된 DNA는 두 전극 중앙에 존재하였다. 또한, 일반 마이카, Mg^2+ 처리된 마이카, 그리고 AP-마이카 표면들에 대한 신장 효율의 차를 기술하였다. 동일한 실험조건 하에서 AP-마이카의 표면이 가장 좋은 효율의 DNA 신장 결과를 나타내었다. An effective method of DNA stretching using electric field on mica surface was proposed for extremely low solution concentration of DNA. DNA stretching based on electric field was observed by AFM and well applied on the concentration range from 57×10 exp(-3)ng/mL to 57×10 exp(-6)ng/mL. Stretched DNA existed in the middle of two electrodes. The difference in stretching efficiency for the different surfaces of bare mica, Mg^2+ soaked mica and AP-mica was discussed. The best performance of stretching was found from the surface of AP-mica under the same experimental condition.

"한국판 학령전기 기질성격검사[K-psTCI]" 의 신뢰도 및 타당도 검사

박진균,김지웅,기선완,신의진 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : The purpose of this study was to evaluate the reliability and validity of the Korean version of Preschool Temperament and Character Inventory (K-psTCI), a questionnaire based on Cloninger's seven-factor model of personality. Methods : The psTCI was translated into Korean and administered to 266 children aged 2-6 years. α test-retest study of the K-psTCI was conducted across a 4-month interval, Internal consistency was calculated by Cronbach a. Test-retest and reliability was analyzed by Pearson correlation analysis. Factor analyses for the temperament and character dimensions were performed using principal component analysis, rotating factors by varimax. The psTCI scores of Korean and United States preschoolers were compared Results : Cronbach α values for the K-psTCI scales ranged from .62 to .78 for each dimensions. Test-retest correlations (r) ranged from .50 to .77 for each dimensions. Explorative factor analysis with the condition of eigenvalue greater than 2 produced four factors for the temperament items and three factors for the character items like the US original version of psTCI. Through factor analyses, five items in the K-psTCI were categorized differently from the US version of psTCI. Korean preschoolers had higher mean scores on Cooperativeness (CO) in males as compared to a sample of US preschoolers. Conclusion : The results of this study suggests that the Korean preschool TCI is satisfactory in reliability and validity.

全身麻醉中 筋弛綬劑가 血淸 Ca^++, Na^+ 및 K^+에 미치는 影響

朴辰雄 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

전신마취시 succinylcholine, d-tubocurarine 및 pancuronium 투여후 10, 20 및 30분의 혈청 Ca^++, Na^+ 및 K^+ 농도를 마취전과 비교하였던 바 다음과 같은 관찰을 할 수 있었다. 탈분극성근이완제인 succinylcholine을 투여한 군에서는 K^+치는 10분에서는 대조치와 비교해서 큰 변화를 볼 수 없었으나 20 및 30분에서는 대조치와 비교해서 의의있는 상승치를 보였고, Na^+치는 대조치에 비해 10분에서 의의있는 감소를 보였고 20분에서 의의있는 상승을 보였으나 30분에서는 별 변화를 볼 수 없었다. Ca^++치는 대조치에 비해 10, 20 및 30분에서 의의있는 감소를 볼 수 있었다. 비탈분극성인 d-tubocurarine 투여후의 Ca^++ 및 K^+치는 대조치와 비교해서 10, 20 및 30분에서 별 변화가 없었으나 Na^+치는 10분과 20분에서 의의있는 감소를 보였고 30분에서는 별 차이를 볼 수 없었다. 비탈분극성인 pancuronium 투여후의 Ca^++, Na^+ 및 K^+치는 대조치와 비슷한 경향을 보였다. The changes of the serum calcium, sodium, and potassiun, concentration after use of succinylcholine, d-tubocurarine and pancuronium were compared before anesthesia with during anesthesia in 60 surgical patients. In succinylcholine administration group, potassium concentration was significantly increased at 20 and 30 minutes and calcium concentration was slightly decreased at 10, 20 and 30 minutes and sodlum concentration was decreased at 10 minutes and increased at 20 minutes slightly. In-d-tubocurarine administration group, sodium concentration was significantly decreased at 10 and 20 minutes, but calclum and potassium concentration were not changed during anesthesia. In pancuronium administration group, calcium, sodium and potassium concentration were not changed during anesthesia.

氣管內揷管時 Lidocaine 前處置가 循環에 미치는 影響

朴辰雄 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

경북대학교 의과대학 부속병원에서 기관내삽관에 의한 전신마취하에 수술을 받을 20세에서 68세의 성인을 대상으로 하여 대조군, lidocaine분무군,lidocaine정주군 및 lidocaine을 경기관내직접주입군으로 관찰하였던 바 다음과 같은 결과를 얻었다. 수축기혈압은 대조군보다 lodocaine정주군 및 분무군에서 삽관후 의의있게 억제(P<0.05 및 P<0.001)되었으며, 경기관내 주입군에서는 삽관후가 마취유도 이전으로 되돌아오는 상태가 빨랐다. 확장기혈압은 대조군보다 각군에서 마취유도시,삽관시 및 삽관후 다같이 의의있게 감소된 것을 알수 있었다(P<0.05 및 P<0.001). 또한 각군 공히 마취유도전으로 회복은 빨리되지는 않았다. 심박동수는 대조군보다 삽관시 분무군 및 경기관내 직접주입시 에서는 의의있는 감소(P<0.05)를 보였고 삽관후는 정주군 및 경기관내 직접주입시에 의의있는 감소(P<0.05)를 보였다. 이상과 같이 lidocaine의 다양한 방법으로의 투여는 삽관시 혈압 상승 및 빈 맥을 어느정도는 감소시켰으나, 완전한 방법은 되지 못했다. Changes in systolic and diastolic blood pressure and heart rate during direct laryngoscopy and tracheal intubation with lidocaine were determined in 136 patients scheduled for elective operation under general anesthesia. These patients were divided into 4 groups each: Group Ⅰ served as a control group. (N=32) Group Ⅱ served as a alidocaine sprayed group. (N=30) Group Ⅲ served as a intravenous lidocain injected group. (N=46) Group Ⅳ served as a transtracheal lidocaine injected group. (N=28) The changes of systolic and diastolic blood pressure and heart rate following intubation were analyzed and compared among the groups. The results were as follows : In the lidocaine intravenously injected or sparayed group during intubation, systolic blood pressure elevation was less magnitudethan the control group (statistically significant) and in the transtracheal lidocaine injected group returned rapidly to the pre-induction level. In the magnitude of diastolic blood pressure ele vation, all the groups were less elevation than the control group and not returned rapidly to the pre-induction level. In the lidocaine sprayed group or transtracheal injected group, the magnitude of heart rate elevation during intubation was less elevation than the control group (statistically significant). In the lidocaine intravenously injected group, the magnitude of heart rate elevation after intubation less than the control group.

자갈함량과 질소시비량이 고랭지 배추재배시 침투수에 의한 질소용탈 및 생육에 미치는 영향

박철수,이계준,정영상,주진호,황선웅 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

고랭지 배추재배지의 성토재로 사용되고 있는 석비레는 모래와 자갈함량이 높아 토양침식과 양분용탈의 주 요인이 되고 있어 본 연구는 자갈함량에 따른 배추수량과 질소의 용탈양상을 구명하고자 pot 시험을 수행하였다. 시험 결과, 배추의 생체수량은 자갈의 함량이 증가할수록 감소하는 경향이었고, 특히 질소시비량 240 kg/ha 구에서는 현저한 수량감소를 보였다. 질소시비량 60 kg/ha 구에서는 자갈함량 30%, 질소시비량 120 kg/ha 구에서는 자갈함량 50%를 변환점으로 하여 배추수량이 급격히 감소하였다. 배추의 생육장해는 질소시비량이 240 kg/ha인 구에서는 대부분 위조현상이 나타났으며 결구 초기인 8월 초순에는 자갈함량이 50% 이상이 되면 질소시비량을 ha당 120 kg만 시용해도 50% 이상이 위조현상을 보였다. 침출수의 암모늄태 질소는 자갈함량과 질소시비량이 많을수록 침출수의 농도가 높아지는 경향을 보였는데, 특히 배추의 고사가 발생한 30% 이상의 자갈함량과 질소시비량 240 kg/ha인 처리구의 농도는 139-339 mg/L이었다. 침출수의 질소용탈량은 자갈함량에 따라 정의 상관관계를 보였고, 자갈함량이 10% 증가될 때마다 pot당 0.14g씩 증가하여 자갈함량이 10%일 때에 비하여 자갈함량이 30%가 되면 약 3.8배 증가하였다. 배추재배 시험 후 토양의 화학성은 NH₄-H 농도와 pH는 처리간에 일정한 경향을 보이지 않았고, NO₃-N 농도는 자갈함량 30%까지는 증가하다가 50%부터는 감소하는 경향을 보였다. 사양토에서 자갈의 함량이 30%일 때 질소비료 240 kg/ha, 자갈함량이 50%일 때 질소비료 120 kg/ha을 시용하면 배추의 생육장해를 가져올 수 있다. 따라서 이러한 토양에 시비를 하게 되면 염류장해와 위조현상을 초래하므로 토양유실이 심한 고랭지는 특수환경을 고려한 시비기준이 설정되어야 할 것으로 판단된다. Continuous monocropping of Chinese cabbage in Gangwon highland increased gravel and sand contents due to surface soil erosion. Nutrient leaching and Chinese cabbage growth were investigated with different treatments of gravel contents and nitrogen application levels by using 0.5 m²Wagner pots. Gravel contents were 0, 10, 30, 50, 70, and 90%(w/w), nitrogen application levels were 60, 120, and 240 kg/ha, and manure compost application rate was 15 ton per hectare, respectively. Wagner pots were filled with loamy sand soil mixed with 5 cm-sized gravels. Fresh weight of Chinese cabbage was decreased as gravel contents in soil increased, and particularly severely decreased at 240 kg-N/ha. Yields of Chinese cabbage were remark-ably decreased at the rate of 60 kg-N/ha with 30% gravel content and 120 kg-N/ha with 50% gravel content. Most of Chinese cabbages were severely wilted by heavy N application at the rate of 240 kg-N/ha in the middle of growth stages regardless of gravel contents, while about 50% of Chinese cabbage showed wilting symptom in the treatment of more than 50% of gravel contents and 120 kg-N/ha. N content in leachate in-creased as gravel content and N application increased. The relationship between gravel content and N con-tents showed linear regression: N in leachate = 0.014(gravel content) -0.039 (r = 0.961). Particularly, NH₄-N contents in leachates with more than 30% gravel content and 240 kg-N/ha ranged from 139~339 mg/L. Chinese cabbage growth in loamy sand soil containing 30%, and 50% gravel contents could be adversely affected by N application at the rate of 240, and 120 kg-N/ha, respectively.