Claudin-1 expression decreases with increasing pathologic grade in actinic keratosis and may be used as a marker of high-risk actinic keratosis

( Ji Su Lee ),( Hyun-sun Park ),( Hyun-sun Yoon ),( Soyun Cho ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Actinic keratosis (AK) is a common sun-induced skin disorder of importance for the progression to invasive squamous cell carcinoma. However, there is still no good way to predict high risk AK. Objectives: To identify markers which reflect the biologic behavior of AK and to understand the pathogenesis of AK Methods: A total of 52 patients with AK and site-matched 17 normal controls were included. We evaluated solar elastosis and immunohistochemical features using the following antibodies: p53, vitamin D receptor (VDR), claudin-1, and Langerin. Results: Solar elastosis increased and Langerhans cell (LC) density decreased with aging in both patients and controls. Solar elastosis and p53 expression were higher and VDR expression was lower in patients than controls; however, they showed no statistical difference in relation to the pathologic grade of AK. Claudin-1 expression gradually decreased from normal control to severe AK and decreased in the areas with epidermal atypia. LC density in severe AK was significantly lower than in normal control and mild AK; no difference in LC density was seen among control, mild AK and moderate AK. Conclusion: Claudin-1 can be a useful marker of pathologic severity of AK. In contrast, p53 increases and VDR decreases in AK not in gradual manner but in the early steps of carcinogenesis. LC density is relatively maintained in AK until it reaches severe dysplasia.

Atypical pigmented basal cell carcinoma mimicking traumatic pigmented tattoo

( Ji Won Kim ),( Hyun-sun Park ),( Hyun-sun Yoon ),( Soyun Cho ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Basal Cell Carcinoma, the most common type of skin cancer in humans, usually develops in the sun-exposed area of head and neck. A 63-year-old woman presented with 1-year history of a bean-sized, slightly protruded bluish dermal patch in her right para-nasal area, and it gradually increased in size after she perceived its presence. It looked like traumatic pigmented tattoo because of bluish color and flat patchy features. A punch biopsy was performed, and the lesion was diagnosed as a basal cell carcinoma (BCC). Histopathologically, melanophages were seen scattered between typical basaloid BCC tumor nests in the reticular dermis, making the lesion appear similar to a traumatic pigmented tattoo by Tyndall effect. Typically pigmented BCC appears as a hyperpigmented, translucenet papule which may also have eroded borders. The patient only showed bluish colored patch without eroded borders, making it appear clinically similar to a pigmented tattoo. Herein, we report an interesting case of pigmented BCC which mimicked traumatic pigmented tattoo but was confirmed to be a BCC by biopsy.

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

자가면역 용혈빈혈의 임상적 분석

박치영,강지은,박상곤,김상현,김형선,정춘해 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background: Autoimmune hemolytic anemia (AIHA) is a very uncommon disorder with the incidence between one and three cases per a hundred thousans a year. There have been very few studies about AIHA in Korea because of its low incidence. Most AIHA studies in Korea were about case history. The aim of this study was to review the clinical features, therapy and outcome of AIHA patients in single institution clinic. Methods: We reviewed retrospectively the medical records of AIHA patients from December 2002 to December 2006. Results: Twelve patient with AIHA were identified, and their median age was 49 years (range 27-75). Male to female ratio is 9:3. The median hemoglobin level was 5.0 g/㎗ (range 2.5-8.2). Warm type AIHA was most common (8/12), cold type was three, and mixed type was one. There was histories of associated disease in six patients. Two patients were complicated by systemic lupus erythematosus and mycoplasma infection and one patient by idiopathic myelofibrosis and solid tumor. Oral steroid was used as the first-line treatment in nine patients. An initial response was observed in eight patients with complete response. One patient had not responded to the initial steroid treatment, and then showed complete response with rituximab. Two relapsed patients were treated with rituximab and partial splenic embolization. Conclusion: The majority of patients can be treated with steroid. Rituximab can improve the response rate in relapsed and refractory AIHA.

간호정보의 처리, 분석, 관리 기술개발-인공신경망을 이용한 간호진단시스템 구현-

유지수,유황빈,박지원,고일선 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.2

쌓기 활동에서 유아들의 친 사회적 행동 출현에 대한 맥락적 이해

박화윤,마지순,최재숙 한국영유아보육학회 2002 한국영유아보육학 Vol.0 No.31

The purpose of this ethnographic study is to explore the emergent context of prosocial behavior and to find the children's character promoting of prosocial that within the area of block. The result are as follows: 1) It appeared that emergent context of prosocial; behavior is completion of structures, participation of symbolic play using them and clean-up. 2) It appeared that children's character promoting prosocial behavior that appearence of altruism by children's perspective-taking, exchage of idea and problem-solving.

현행 고등학교 중국어 교과서에 게재된 사진의 문화적 고찰

朴現圭,陳秀美,柳知惠,金善兒 한국중국문화학회 2001 中國學論叢 Vol.12 No.-

自從1992年8月, 韓中兩國建交以后, 在外交·商務·文化交流1留學·旅游等各個方面都得到了蓬勃的發展. 爲了順應這一國際潮流, 最重要駒就是語言能力. 因此, 敎育部的敎育課程也增大了對中國語敎育的關心, 事實上在高中敎育中, 將中國語選爲第二外國語的比率呈逐漸增加的縕勢. 在外國語敎育中, 作爲基本的敎材的作用不僅在外國語敎育方面, 而且還注重于對使用相應語言的國家和民族的文化進行理解. 因此, 最近爲了在中國語敎育課程中也强化有關中國文化敎育的重要性, 在敎材課文中直接載入中國文化以及韓中兩國文化之뇌較等內容, 而且在敎材中還附加照片和揷鬪資料, 幷行視覺敎育 通過這-方法, 學生在首次接觸中國語敎材時, 通過封面和封面內的照片, 以及本文中的據獨照片, 可以更加准確地把握何霜中國的形象和文化.本論文正是着眼于這-點, 選擇第雌騙驗育課程中被選定的一般高中現行中國語敎材共5冊, 以其中所登載的照片爲對象進行分析. 同時分析內容還包括, 讀些照片對于學生來說, 在了解中國文化方面如何有效地傳達知識, 在增進理解方面會起到多大作用, 幷旦對這里所存在的間題進行討論, 探求合理的改善方案. 書本是內容的充實也固然重要, 但外部的設計以及附加照片 1 揷圖等視覺效果也不容忽視, 具有同等的重要性. 一般的書籍如此, 而作爲敎材來講, 在增進學習效果方面, 這種視覺效果更重要. 本論文雖然以對現行中國語敎材中所收錄駒部分照片爲進行了分析, 但在誤計彧圖片部分也可作與沈相同約結論. 2002年敎育課程的轉換時機卽將來臨, 中國語敎材也重新進拒了改編. 希望今盾被改編的中國語敎材將對本論文中所提及的問題進行改善, 對書中的每一張照片都進行愼重的選擇, 從而可使學習中國語的學生更加准確多方面地了解中國文化. 爲了對這些間題進行改善, 筆者認爲敎育當局應該投入更多的關心, 幷在制度上予以充分的協助.

물류시스템 최적화를 위한 설비배치에 관한 연구

박세준,이영해,허선,이문환,유지용 한국경영과학회 1998 한국경영과학회 학술대회논문집 Vol.- No.2

We consider a facility layout problem for optimized material flow system design in the automated production system design. Existing facility layout algorithm have a weak point that arranged facilities have irregular shape or don't preserve their own shape and size. The proposed algorithm give a layout which is minimize total material handling cost and maximize space utility under preserving each facilities own shape and size.

노인용 게이트볼웨어 개발에 관한 연구

박지윤,최혜선 이화여자대학교 생활환경대학 인간생활환경연구소 2005 인간생활환경연구소 논집 Vol.- No.4

The purpose of this study is to develop an improved summer Gateball wear that will be much more comfortable and convenient than what is currently worn for the game by those between the ages of 60s and 70s. The investigation has been conducted to evaluate current clothing to find out any problems and discomforts related to the current clothing. For accurate data, hands-on investigation and a short interview have been performed. The questionnaires were answered by 230 persons. The result of the questionnaire enables us to design a sample Gateball wear. material, pattern and design have been improved. A sample Gateball wear was evaluated through a wearing test and an appearance test to compare it to current Gateball clothing. The sample gate-ball wear received more positive assessment than the current clothing.