연구논문 : 만성 바이러스간질환에서 간섬유화의 다양한 비침습 혈청표지자검사의 비교

김선민 ( Sun Min Kim ),손주현 ( Joo Hyun Sohn ),김태엽 ( Tae Yeob Kim ),노영욱 ( Young Wook Roh ),은창수 ( Chang Soo Eun ),전용철 ( Yong Cheol Jeon ),한동수 ( Dong Soo Han ),오영하 ( Young Ha Oh ) 대한간학회 2009 Clinical and Molecular Hepatology(대한간학회지) Vol.15 No.4

목적: 만성 바이러스간질환에서 병의 경과와 예후를 평가하고 치료반응을 예측함에 있어 간섬유화의 진단은 중요하다. 최근 간섬유화 평가에 표준방법이지만 침습적 간생검을 대신하여 여러 가지 비침습적 진단법이 이용되고 있고, 또한 새로운 검사법들이 개발되고 있다. 저자들은 만성 B형 및 C형간염 환자를 대상으로 임상에서 광범위하게 흔히 시행하는 혈액검사와 임상 소견을 이용한 아래와 같은 진단법들만으로 간섬유화를 얼마나 정확하게 평가할 수 있는지 알아보고, 단일 검사로 간섬유화를 평가하는 데 유용하다고 알려져 있는 hyaluronic acid(HA)와 IV형 콜라겐 농도와 비교하여 그 임상적 유용성을 평가하였다. 대상과 방법: 2002년 3월부터 2007년 2월까지 만성 바이러스간 질환으로 간생검을 시행받은 225명의 환자(HBV 180명, HCV 43명, HBV+HCV 2명)를 대상으로 분석하였다. 간생검을 시행하는 날에 말초혈액검사, 혈액응고검사, 혈청생화학검사를 시행하고, 혈중 HA, IV형 콜라겐 농도를 측정하였다. 간섬유화의 정도는 F0(섬유화가 없음), F1(문맥역 섬유화), F2(문맥주변부 섬유화), F3(섬유성 격막) 및 F4(간경변증)의 4단계로 구분하였다. 대상 환자를 F0-1, F2-4 혹은 F0-2, F3-4의 두 집단으로 분류하여 두 집단을 구분하고자 할 때 AAR(AST/ALT ratio), API(age-platelet index), APRI(AST to platelet index), CDS(cirrhosis discriminant score), platelet count, HA, IV형 콜라겐의 예측능을 area under the receiver operating characteristic curve (AUROC)값을 이용하여 비교하였다. 결과: 대상환자의 섬유화의 단계는 F0 집단은 17명, F1 집단은 40명, F2 집단은 61명, F3 집단은 74명, F4 집단은 33명이었다. 의미 있는 간섬유화를 F2 이상으로 판단할 때, 의미 있는 섬유화의 예측에 대한 AUROC 값은 APRI=0.822, CDS=0.776, platelet count=0.773, API=0.756, HA=0.749, IV형 콜라겐=0.718, AAR=0.642 순이었고, F3 이상의 광범위한 섬유화 예측에 대한 AUROC값은 CDS=0.835, platelet count=0.795, API=0.794, HA=0.766, AAR=0.711, IV형 콜라겐=0.697, APRI=0.691 순으로 관찰되었다. 결론: 만성 바이러스간질환에서 간섬유화를 평가하는 데 임상 소견 및 혈액검사를 이용한 방법들이 비침습적 진단법 중 단독 검사로도 유용한 것으로 알려져 있는 혈중 HA와 IV형 콜라겐 농도와 비교하여 우월하거나 대등하였다. 특히 APRI는 다른 인자들에 비해 F2 이상의 의미 있는 섬유화를 예측하는 데 가장 유용하였고, CDS는 F3 이상의 광범위한 섬유화를 예측하는 데 가장 유용하였다. Background/Aims: The aim of this study was to determine the clinical performances of noninvasive serum markers for the prediction of liver fibrosis in chronic viral liver diseases. Methods: We analyzed a total of 225 patients with chronic viral liver diseases (180 with hepatitis B virus, 43 with hepatitis C virus, and 2 with hepatitis B+C virus) who underwent a liver biopsy procedure at the Hanyang University Guri Hospital between March 2002 and February 2007. Serum was also obtained at the time of liver biopsy. Liver fibrosis was staged according to the scoring system proposed by the Korean Study Group for the Pathology of Digestive Diseases. Various noninvasive serum markers were evaluated, including the aspartate aminotransferase (AST)/alanine aminotransferase (ALT) ratio (AAR), age-platelet (AP) index, AST/platelet ratio index (APRI), cirrhosis discriminant score (CDS), platelet count, hyaluronic acid (HA), and type IV collagen. Results: There were 17, 40, 61, 74, and 33 patients at stages F0, F1, F2, F3, and F4, respectively. The overall diagnostic accuracies of each marker, as determined by the area under receiver operating characteristics curves, were APRI=0.822, CDS=0.776, platelet count=0.773, AP index=0.756, HA=0.749, type IV collagen=0.718, and AAR=0.642 for predicting significant fibrosis (≥F2); and CDS=0.835, platelet count=0.795, AP index=0.794, HA=0.766, AAR=0.711, type IV collagen=0.697, and APRI=0.691 for predicting extensive fibrosis (≥F3). Conclusions: Conclusions: All noninvasive serum markers evaluated in this study were useful for predicting significant or extensive liver fibrosis in chronic viral liver diseases. In particular, APRI was most useful for the prediction of significant fibrosis, and CDS was most useful for the prediction of extensive fibrosis. (Korean J Hepatol 2009;15:454-463)

수직적 치조제 증강술후 발생하는 골흡수량에 관한 연구

전하룡(Ha-Ryong Jeon),김종원(Jong-Won Kim),권호범(Ho-Beom Kwon),이동환(Dong-Hwan Lee),홍종락(Jong-Rak Hong),김창수(Chang-Soo Kim) 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.3

Purpose: This study presents radiographic and laboratory analysis and comparison of bone resorption rate of grafted endochondral bone and intramembranous bone on the aspect of height and volumetric change. Patients and Methods: 18 partially edentulous patients who needed alveolar ridge augmentation for implant placement during the years 2002 to 2004 were selected for this study. Group A consisted of 5 males & 3 females who were treated with intraoral(intramembranous) bone and Group B consisted of 8 males & 2 females who were treated with iliac(endochondral) bone. Non-standard periapical X-ray was taken at day 1, 2 month, 4 months, 8 months after the surgery. Resorption rate of grafted bone were measured on these X-rays and compared. Also we calculated volume of grafted bone with models which was fabricated at 1.5 months, 6 months. Results: There was bone resorption in both groups. Group B showed more bone resorption than Group A. In Group A, the resorption rate according to the radiographic measurements was 9.81 %, and resorption rate according to volumetric measurement was 16.5 %. In group B, the resorption rate according to the radiographic measurements was 15.9 %, and resorption rate according to volumetric measurement was 30.6 %. Significant difference is on radiographic resorption of post-op 2, 4, 8 months on two groups (P < 0.05). Also significant difference is on volume resorption on two groups (P < 0.05) Conclusion: We found that more bone resorption occurred with iliac(endochondral) bone and when we use intraoral bone, that bone can maintain their vitality for alveolar ridge augmentation.

위장관 암환자들에서 관찰한 혈중 총항산화능(Total Antioxidant Capacity)의 변화

전창호,이은하,이한일 대한임상병리학회 1998 대한임상병리학회지 Vol.18 No.2

마이크로파 여기 플라즈마광 생성을 위한 도파관 시스템 설계에 관한 연구

전상재,전후동,송창현,하석영,이승혁,이태호,박의준 금오공과대학교 2005 論文集 Vol.26 No.-

In this paper, the plasma lighting system(PLS) excited by the commercial high power magnetron is developed. The design concepts are based on maximizing the huninous efficacy in conjunction with the miniaturization of waveguide system Furthermore the fine tuning is simplified by using only one stub, and the impedance matching is maximized by introducing the tapering technique. The experimental results show that the luminous efficacy can be dramatically improved by the proposed design method.

자동화학 분석기 ISP-1000의 평가 Ⅱ

김문연,고은하,하경임,김지용,전창호 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

검사방법의 평가로서 자동화기기에 대한 적절한 평가는 특히 수입에 전적으로 의존하는 국내여건을 생각할때 중요한 과제이다. ISP-1000은 대용량, 다항목의 선택적 생화학 자동 분석기로 국내에서는 거의 채택된 경험이 없는 기기로서 효율적인 선정을 하기위하여 4가지 화학검사에 대한 평가를 하였다. 시판되는 대조물질 Level Ⅰ,Ⅱ로 검사내 정밀도, 검사일간 정밀도, 직선성을 구하였으며 100명의 환자검체로는 Cobas Mira와의 상관성을 구하였다. 검사내 정밀도는 Level Ⅰ대조물질에서는 변이계수가 4.31-8.73%, Level Ⅱ 대조물질에서의 변이계수는 1.32-8.22%이었으며, 검사일간 정밀도는 Level Ⅰ 대조물질로 얻은 변이계수는 6.29-8.974%, Level Ⅱ 대조물질로 검사한 경우는 5.78-8.22%이었다. 직선성의 평가에서는 0.9917-0.9998의 우수한 직선성을 보여주었고(P<0.01), Cobas Mira에 대한 상관관계는 0.8427-0.9857의 범위에 있었다(P<0.01). 결론적으로 ISP-1000에 대한 4가지 화학검사의 평가에 있어서 검사중 정밀도, 검사일간 정밀도, 직선성은 우수하였으며, 상관관계는 만족할만하였으나 더 좋은 결과를 얻기위하여 parameter등에 대한 재검토가 필요한 것으로 생각되었다. It is an important task to evaluate autochemical analyzers as one of the labortory method evaluations, especially considering of the domestic environments almost depending on the import. By analyzing 4 chemical tests, we evaluated the high throughoutput, multichannel, selective and rarely adopted chemical autoanalyzer ISP-1000. We analyzed within run precision, between day precision, lineality with the commercial control materials, and correlation with 100 patients samples. In within run precision, CVs were ranged from 4.31~8.73% in Level I control material, l.32-8.22% in Level Ⅱ control material. In between day precision, CVs were ranged from 6.29-8.974% in Level I control material, 5.78-8.22% in Level Ⅱ control material. The lineality was excellent(r²>O,9917, p<O.Ol). The correlation coefficients between ISP-l000 and Cobas Mira were ranged from 0.8247∼0.9857%(P<O.0l). In conclusion, 4 chemical tests of ISP-l000 were acceptable in within run precision, between day precision and lineality, and correlation with Cobas Mira was good but adjusting the parameters would be necessary for the better results.

Evaluation of Agronomic Stability of North Korean Rice Varieties using Statistical Models

O-Young Jeong,Jeom-Ho Lee,Ha-Cheol Hong,Eung-Gi Jeong,Jin-Soo Paek,Chang-Ihn Yang,Yong-Hee Jeon,Myeong-Ki Kim,Kyu-Seong Lee,Sae-Jun Yang,Young-Tae Lee 韓國作物學會 2008 한국작물학회지 Vol.53 No.1

This experiment was carried out to evaluate the agronomic stability of North Korean rice varieties using the statistical model developed by Grafius, Finlay, and Ever hart. The lowest yearly variation based on coefficients of variation was found in Hannam 29 for number of panicles per hill, in Sijoong 9 for number of grains per panicle, in Pyeongyang 3 for ripened grain ratio, in Sijoong 16 for 1,000 grain weight, and in Yeomju 1 for grain yield. By Grafius's model, Pyeongbook 3, Weonsan 66 in early maturing groups and Seohaechalbyeo in medium maturing groups show stable for 3 years. Weonsan 66 in early maturing groups and Seohaechalbyeo in medium maturing groups were found to be highly stable as analyzed by both Finlay and Wilkinson's model and Everhart & Russell's model. With reference to three models, Weonsan 66 was highly stable for 3 years with showing more yield than Odaebyeo in early maturing groups while Seohaechalbyeo was highly stable for 3 years with showing high yield than Hwaseongbyeo in medium maturing groups above 5~;t~;ha-1 of milled rice respectively.

마이크로 엔드밀링에서 음향방출 신호를 이용한 상태감시

정연식,강익수,김전하,강명창,김정석,안중환 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

Ultraprecision machining and MEMS technology have been taken more and more important position in machining of microparts. Micro endmilling is one of the prominent technology that has wide spectrum of application field ranging from macro parts to micro products. Also, the method of micro-grooving using micro endmilling is used widely owing to many merit, but has problems of precision and quality of products due to tool wear and tool fracture. This investigation deals with state monitoring using acoustic emission(AE) signal in the micro-grooving. Characteristic evaluation of AE raw signal, AE hit and frequency analysis for state monitoring is also presented in the paper.

Evaluation of Agronomic Stability of North Korean Rice Varieties using Statistical Models

Jeong, O-Young,Lee, Jeom-Ho,Hong, Ha-Cheol,Jeong, Eung-Gi,Paek, Jin-Soo,Yang, Chang-Ihn,Jeon, Yong-Hee,Kim, Myeong-Ki,Lee, Kyu-Seong,Yang, Sae-Jun,Lee, Young-Tae The Korean Society of Crop Science 2008 한국작물학회지 Vol.53 No.1

This experiment was carried out to evaluate the agronomic stability of North Korean rice varieties using the statistical model developed by Grafius, Finlay, and Ever hart. The lowest yearly variation based on coefficients of variation was found in Hannam 29 for number of panicles per hill, in Sijoong 9 for number of grains per panicle, in Pyeongyang 3 for ripened grain ratio, in Sijoong 16 for 1,000 grain weight, and in Yeomju 1 for grain yield. By Grafius's model, Pyeongbook 3, Weonsan 66 in early maturing groups and Seohaechalbyeo in medium maturing groups show stable for 3 years. Weonsan 66 in early maturing groups and Seohaechalbyeo in medium maturing groups were found to be highly stable as analyzed by both Finlay and Wilkinson's model and Everhart & Russell's model. With reference to three models, Weonsan 66 was highly stable for 3 years with showing more yield than Odaebyeo in early maturing groups while Seohaechalbyeo was highly stable for 3 years with showing high yield than Hwaseongbyeo in medium maturing groups above $5\;t\;ha^{-1}$ of milled rice respectively.

AE 신호를 이용한 회전형 압축기의 이상상태 감시

이감규,정지홍,김전하,강명창,김정석 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.5

The compressor has one of important roles in refrigeration cycle and it determines refrigeration efficiency and quality. This paper aims to monitor rotary compressors for room air conditioners by using Acoustic Emission(AE) technique. The reliability of rotary compressors has been evaluated through visual inspection on them after long term test. This paper describes methods for acquisition and processing AE raw signal to monitor the state of rotary compressor. A detecting method of abnormal compressor in real time is suggested and special-purpose monitoring system which can be applied to automatic manufacturing line is developed using one-chip microprocessor at low cost.

폐경후 여성에서 지속적 복합 호르몬대체요법시 Medroxyprogesterone Acetate가 혈중 지질대사에 미치는 영향

박만철,이용우,이경희,김정욱,전현아,장전호,김병석,윤종률 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.5

에스트로겐 호르몬대치요법시 자궁내막의 증식을 예방하기위해 병용투여하는 황체호르몬제제 중 비교적 안드로젠 성향이 낮은 MPA를 지속적 호르몬대치요법으로 사요하여 적은 용량으로 매일 투여할 경우, MPA가 혈중지질 및 지단백질대사에 미치는 영향을 알아보고자, 자궁이 있는 폐경후 여성 15명에게 CEE0.625 mg과 MPA 2.5 mg을 6개월간 매일 지속 투여하였으며, 대조군으로 자궁의 양성 질환으로 자궁적출술 및 양측 난소절제술을 시행받고 폐경 상태인 16명의 여성에게 CEE 0.625 mg을 단독 투여하여 호르몬제제 투여전과 투여후의 혈중 지질 및 지단백질치를 측정하여 비교한 결과는 다음과 같았다. 1. 혈중 총 콜레스테롤치는 대조군과 MPA병용투여군 모두에서 호르몬대치요법 시행 후 통게학적 유의성 있는 감소를 보였다. 2. 혈중 고밀도 지단백질 콜레스테롤치는 두 군 모두에서 호르몬대치요법 시행 후 통계학적 유의성 있는 증가를 보였다. 3. 혈중 저밀도 지단백질 콜레스테롤치는 호르몬 대치요법 시행후 두 군 모두 유의성있는 감소를 보였다. 4. 혈중 중성 지방치는 두 군 모두 호르몬대치요법 시행 후 유의성있는 증가를 보였다. 5. 두 군간의 호르몬대치요법 시행후의 변화는 통계학적 유의성 있는 차이를 보이지 않았다. 이상의 결과로 보아, 호르몬대치요법시에 자궁내막의 증식을 예방하고 환자의 순응도를 높이기 위하여 지속적 호르몬요법시 사용하는 저용량인 MPA 2.5 mg의 지속적인 투여는 에스트로젠에 의한 혈중 지질 및 지단백질의 유익한 변화를 상쇄시키지 않는 것으로 사료된다. 도한 이후로 MPA를 더 오랜 기간 사용할 경우 혈중지질 및 지단백질에 미치는 효과와 MPA외의 다른 황체호르몬제제(특히, natural progesterone인 utrogestan)를 사용할 경우 혈중 지질 및 지단백질치의 변화는 어떠한가에 대한 비교연구 및 각각의 황체호르몬제제가 한국인 여성의 골밀도에 미치는 영향에 대한 연구가 필요하다고 사료된다. This study evaluates the impact on serum lipid and lipoproteins of adding continuously medroxyprogesterone acetate to conjugated equine estrogens in postmenopausal women. That is whether concomitant progesterone administration opposes beneficial effect of estrogen on lipoproteins whiles reducing the incidence of endometrial hyperplasia and cancer, or not. We studied on 31 menopausal women who had never hormonal therapy and no history of stroke, diabetes or any other cardiac, hepatic or renal disorker. Group A patients(n=16) were castrted postmenopausal women for at least 1 year and were given daily conjugated equine estrogen 0.625 mg for 6 months. Group B patients(n=15) received daily conjugated equine estrogen 0.625 mg and medroxprogesterone acetate 2.5 mg for at least 6 months. Pre- and post-treatment evaluation of serum lipoprotein concentration demonstrated significant declines in total cholesterol(10.35% and 12.34%) and low-density lipoprotein cholesterol(24.21% and 28.13%) and significant increases high-density lipoprotein(16.18% and 11.69%) and triglyceride(23.59% and 25.40%) within group A and group B(p$lt;0.05). And no differences were noted in the degree of changes of post-treatment serum levels in total cholesterol, high-density lipoprotein cholesterol, triglyceride between two groups. In conclusion, medroxyprogesterence acetare at low doses in continuensly combinded administration in estrogen replacement therpy dose not oppose the beneficial estrogen-induced lipoprotein changes.