생쥐 위장관의 Interstitial cells of Cajal(ICC)에 대한 면역조직화학적 연구

김영철,차경훈,신무경,임건한,김주영,안병수,김장만,양경철,박도영,오재욱,장인엽 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Interstitial cells of Cajal(ICC) are the pacemakers in gastrointestinal tract that modulates gastrointestinal motiliey and these cells also transmit neural input from enteric nerves to smooth muscles. Recent work on tissues from patients with motility disorders that suggest that loss or defect in ICC could be related to pathophysiology in human and animal models. Immunolabelling of ICC in intestinal wall is recently developed by using specific marker, anti-c-kit antibody. Immunohistochemistry was done for ICC network in attempt to provide a morphological basis for the mechanism regulating gastrointestinal motility Methods : Cryosection was done, and whole-mount preparations of mouse stomach, gastrointestinal tract were immunolabelled using the anti-c-Kit. Immunolabelled ICC networks were observed under a confocal laser scanning microscopy. Results : According to three dimensional reconstruction study, we found that the c-Kit-positive celluar networks were widely distributed in the gastrointestinal muscle (1) circular muscle layer(IC-IM), (2) myenteric plexus(IC-MY), (3) deep muscular plexus(IC-DMP) in ileum, (4) submucosal plexus(IC-SMP) and longitudinal muscle layer(IC-LM) in colon. Conclusion : The characteristic profiles of ICC celluar networks provide a morphological basis upon the mechanism regulating gastrointestinal motility. Additional studies for the enteric nerves-ICC interaction are need to evaluate the detailed roles of Icc in gastrointestinal tract.

아동의 사회적 문제해결능력과 어머니의 대화형태와의 관계연구

김재림,백은주,신유림,심미경,이혜경 연세대학교 생활과학대학 1985 婦學 Vol.18 No.-

The present study investigated the relationship between the social problem solving ability of children and the language patterns of their mothers. The subjects included 193 nine year­olds (185 girls, 108 boys), and 230 twelve year­olds (115 girls, 115 boys). The instruments used were a language patterns test for the mother and IPA (Interpersonal Problem Solving Ability Test) for children. Major finding from this study were: ⑴ the correlation between the social problem solving ability of children and the language patterns of their mothers was not statistically significant, ⑵ the language patterns of the mothers were significantly different by child's sex; for boys, mother used more ordered language patterns and for girls, mothers used more ordered language patterns and for girls, mothers used more human­oriented language patterns, ⑶ the social problem solving ability of children were significantly different by child's age; 12­year­olds exhibited higher ability than 9­year­olds.

견/합성섬유 혼방품의 1욕염색(Ⅶ) : 견/아크릴로니트릴 섬유 혼방품의 염색거동 Adsorption Behavior of Silk/Acrylonitrile Fiber

金公朱,김경수,전재홍,이화선 全北大學校 1995 論文集 Vol.40 No.-

In one bath dyeing system of silk/acrylonitrile(acryl) fabric with acid/disperse dyes and acid/cation dyes, adsorption behavior of acid dyes, disperse dyes and cation dyes on silk and acryl fabrics were examined. In the dyeing of acryl with the C. I. Disperse Red 19(Red 19) and The C. I. Disperse Red 60(Red 60), dye uptake with Red 19 was higher than that with Red 60. When the silk and acryl fabric were dyed with C. I. Basic Red 27(Red 27), dye uptake on the acryl was higher than that on the silk. When the silk/acryl fabric was dyed with Red 19 and Basic 27, solid shade could be obtained with Red 19 but could not be obtained with Basic 27. In the dyeing of silk/acryl fabric dyed with Blue 80/Red 27 and C. I. Acid Blue 113(Blue 113)/C. I. Basic Blue 116(Blue 116), compatibility could be obtained with Blue 80/Red 27 but that could not be obtained with Blue 113/Blue 116.

초등학교 아동의 비만지수와 신체활동에 관한 조사연구

신재신,김명희,박형숙,송미경 韓國學校保健學會 2000 韓國學校保健學會誌 Vol.13 No.1

This study researches an obesity inde( and the reaiities of physical activity of etementary school childrnl and pvide basic data to instruct correct physical activity for low weight md obese childraL The subject was 813 Elementary School Chiliherl of grade 4-6 in Krimgiu and data were collected with a Physical Activir Questionnaire from fTs, USA The collected data ware an3ly2ed with real number, pnfentagf avErge and standard deviation techniques. The rarlge was by SPSS WIN. Both of them were in the normal range, between -38.3 and 77.7, between low weight and obesity The results are as follows : 1. An Obesity index according to grade and sex: the average obesity index of 423 boys was 7.56 ± 15.7, for girls it was 2.56± 14.3. Both of them were in the nrnmal range, between -38.3 and 77.7. There were low weight(-38.85±0.9) students as well as obese, including extremely low weight children (-38.85 ±0.9) and extremely obese children. 2. On the whole, in the Freouency of the 25 item physical activities breakdown, children reported to watch TV 1.(2.7±0.6), or do homework(2.1 ±0.9), more than three-fourths of the week, male children, however, played more football(2.0 ±0.9). As male obese children do less MET5, MET8(highly active activity) than male normal children, it was also discovered that low weight children do mnre severe activity in MET8(high activity). In female cases, it was revealed that neither do highly intense activity. 3. In physical activitiy, male children received higher points than fonale children with 92.94±37.95 to 74.46 ±29.50. On the whole, male and female obese children had lower score than normal children. 4. As for attitude toward physical activiv, male children(31.9614.37) had a positive attitude than female children (29.57 ±3.89), but obese male children had a negative attitude regarding activity viewed as normal. ThaefgrE in peventing obesity in elementary school childrerl the treating of under-weight childrern. should be included in the obesity Fgram so as to Pevent mal-nutrition or denciency. It is needed to instruct obese childrul to carry out interlsive w[rk-outs at least 3 times a weft through systematic grams. It is also needed that parents and teachers educate elementary school chil(hell from an early age with iDcimation on the frequency and interlsity of work-outs to prevent obesity. It is also desirable that children be eddcated to cauy out physical activities that address thetr specific needs. As a result of this study, obesif management must be started from elementary school and children should be instructed to act in hiRh-interlsity actirities when they spend leisure time.

A Functional Polymorphism in the CHRNA3 Gene and Risk of Chronic Obstructive Pulmonary Disease in a Korean Population

유승수,( Hyo Gyoung Kang ),( Jin Eun Choi ),( Hyo Sung Jeon ),( Jae Hee Lee ),( Shin Yup Lee ),( Seung Ick Cha ),( Chang Ho Kim1 ),( Jae Yong Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Purpose: A genome-wide association study has identified the 15q25 region as being associated with the risk of chronic obstructive pulmonary disease (COPD) in Caucasians. This study intended as a confirmatory assessment of this association in a Korean population. Methods: The rs6495309C>T polymorphism in the promoter of nicotinic acetylcholine receptor alpha subunit 3 (CHRNA3) gene was investigated in a case-control study that consisted of 406 patients with COPD and 394 healthy control subjects. Results: The rs6495309 CT or TT genotype was associated with a significantly decreased risk of COPD when compared to the rs6495309 CC genotype (adjusted odds ratio=0.69, 95% confidence interval=0.50-0.95, P=0.02). The effect of the rs6495309 C>T on the risk of COPD was more evident in moderate to very severe COPD than in mild COPD under a dominant model for the variant T allele (P-value of test for homogeneity=0.02). Conclusions: We confirmed the association between the 15q25 region and the risk of COPD in a Korean population.

경피적 겸자 정복 및 골수강내 금속핀 고정을 이용한 쇄골 골절의 치료

홍기도,하성식,정남식,심재천,김경호 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적 : 경피적 겸자 정복 및 골수강내 금속판 고정술을 이용한 쇄골 간부 골절의 치료에 대해 유용성을 규명해 보고자 하였다. 대상 및 방법 : 2002년 1월부터 2003년 7월까지 본원에서 쇄골 간부 골절 환자 중 신경 및 혈관 손상이 없었고, 골절의 분쇄상이 비교적 적었던 총 16례를 대상으로 겸자를 이용한 경피적 정복 후에 골수강에 금속판을 삽입하여 고정하였다. 술 후 유합 정도 및 결과를 임상적, 방사선학적으로 분석하였다. 결과 : 방사선학적으로 골유합을 보인 경우가 총 15례였으며, 술 후 평균 9.1주에 유합 소견을 관찰하였다, 임상적으로 강 등의 기준에 따라 구분한 결과, 우수 이상이 14례로 나타났다. 1례에서 1주 만에 K-강선의 내측전위로 골절부위가 재전위되어 관혈적 정복 및 금속판 내 고정을 시행한 이외에 다른 주요 합병증은 없었다. 결론 : 저자들의 방법은 수술시간이 짧고 간단하며, 연부조직의 추가적 손상이 적어 골유합율이 높고, 수술 반흔을 남기지 않아 수술적 적응을 신중히 고려한다면 쇄골 간부 골절의 치료법의 하나로 유용할 것으로 사료된다. Purpose: To investigate the utility of surgical treatment of clavicle shan fracture using a percutaneous towel clip reduction and intramedullary fixation. Materials and Methods : This study was conducted for total 16 case of patients who had no neurovascular injury and a few comminuted bone fragment among patients with clavicle shaft fracture from January 2002 to July 2003. The method of operation was percutaneous towel clip reduction and intramedullary fixation. The clinical and radiological results were evaluated. Results : Radiologically, 15 cases showed bone unions and the avenge time was 9.1 weeks. According to Kang's criteria clinically, there were 14 cases which were more than an excellence. One case substituted open reduction and nailing fixation due to a medial migration of K-wire and re-displacement of fracture even in 1 week. However, there wasn't any other major complication. Conclusion : Due to its having no additional injury to soft tissues, no scar formations, and its short operation time, percutaneous towel clip reduction and intramedullary fixation will be very useful as one of the treatments of clavicular shaft fracture if it follows correct surgical indications.

Isolation of a Bacillus amyloliquefaciens EMD17 with Antimicrobial and Fibrinolytic Activity from Cheonggukjang

Jae Yong Lee,Min Jeong Cho,Kye Man Cho,Chang Kwon Lee,Gyoung Min Kim,Jung-Hye Shin,Jong Sang Kim,Jeong Hwan Kim 한국식품영양과학회 2013 한국식품영양과학회 학술대회발표집 Vol.2013 No.11

경추손상환자에서의 임상적 분석과 치료의 고찰

김경범,최휴진,신현철,김익수,김기욱,허재택,김형동 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.4

Simple and Rapid Prostate Specific Antigen (PSA) Spot Test for Initial Screening of Prostate Cancer

Kim, Wun Jae,Lee, Sang Cheol,Lee, Hyung Lae,Kim, Yong Tae,Lee, Seok Young,Lee, Gyoung Gyou,Han, Kwang Hee,Shin, Kwan Hee,Lee, Seung Chan,Jang, Hoon,Kim, Tae Hwan 충북대학교 의학연구소 2002 忠北醫大學術誌 Vol.12 No.1

연구 목적: 증상이 없는 고령의 남성에서 전립선특이항원 (PSA) 검사가 전립선암의 조기 발견 및 치료를 통해 전립선암의 사망률을 감소시킨다는 데에는 논란의 여지가 있다. 또한 검사는 과도하고 불필요한 추가rja사를 하게 함으로서 환자에게 경제적 부담을 줄 수 있다. 따라서 선별검사로서 기존의 PSA 분석보다 값 싼 검사법이 필요하다 할 수 있겠다. 이에 저자들은 PSA spot 검사가 전립선암의 초기 선별검사로서 유용성이 있는지를 알아보았다. 재료 및 방법: 210개의 혈청에 대하여 PSA spot 검사 및 고전적인 단일클론항체를 이용한 PSA 면역분석 (monoclonal antibody-based PSA immunoassay)으로 PSA 수치를 측정하였다. PSA spot 검사는 면역모세관 분석(immunochromatographical assay)으로 기구는 sample well 및 2개의 반응 구획으로 구성되어 있으며 혈청 4 방울을 sample well에 점적 후 10분 내에 결과를 확인할 수 있다. 이 검사는 PSA 의 기준치를 4 ng/ml로 하였으며 양성인 경우 PSA 치가 4 ng/ml 이상임을 의미한다. 결과: PSA 4.0ng/ml 이상인 혈청 검체의 96% (132/137)가 PSA spot test 양성으로 나타났으며, 4.0 ng/ml 이하인 혈청 검체의 93% (68/73)가 음성으로 나타나 전체적인 일치율(concordance rate)은 95%였다. 52명의 여성 혈청 전부에서 가양성은 나타나지 않았다. 결론: PSA spot 검사는 PSA 4.0ng/ml을 기준으로 양성 여부를 판단하였으며 95%의 민감도 및 특이도를 나타내었다. PSA spot 검사는 간단하면서도 10분 내에 결과를 확인할 수 있는 신속한 검사법이다. 따라서 전립선암의 초기 선별검사로서 추후 검사의 필요성 여부를 결정할 수 있을 것으로 생각된다. Purpose : The routine use of PSA testing in asymptomatic men, as a means for reducing prostate cancer mortality by early detection and treatment, is controversial. PSA testing may result in further tests that are unnecessary and costly without proof that screening reduces prostate cancer mortality. Therefore, a less expensive but accurate test other than the conventional PSA assay would be favorable for initial screening purposes. We evaluated whether the PSA spot test could be used as an initial screening test for prostate cancer. Materials and Methods: PSA levels of 210 serum samples were simultaneously determined by the PSA Spot test and by a classic monoclonal antibody-based PSA immunoassay. The test was also performed in 52 female serum samples as negative controls. The PSA Spot test is an immunochromatographic assay. The device consists of 3 sections, a sample well and 2 reaction zones. Results are read within 10 minutes after applying 4 drops of serum to the sample well. This test is designed to have a detection limit at about 4 ng/ml of PSA. Any positive result would be indicative of a PSA level of 4 ng/ml or above. Results: Overall concordance rate was 95%, and 96% (132/137) of all samples with a PSA value 4.0 ng/ml were positive in the PSA Spot test while 93% (68/73) of samples with PSA <4.0 ng/ml were negative. None of 52 female sera showed false positive with the test. Conclusion: The PSA Spot test was able to distinguish serum with PSA concentration below and above 4.0 ng/ml with sensitivity and specificity of approximately 95%. The test is simple and results can be obtained in 10 minutes. On a single visit to the clinic, all essential information is generated to decide whether further evaluation tests would be needed or not.

Characterization of a 27kDa Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH51 Isolated from Cheonggukjang

Gyoung Min Kim,Ae Ran Lee,Kang Wook Lee,Ae Yong Park,Ji Yeon Chun,Jae Ho Cha,Young Sun Song,Jeong Hwan Kim 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.9