Ciprofloxacin의 돼지 호흡기 감염증 및 설사증에 대한 치료효과

전무형,김지영,채미경,서상희,김명철,박창식,김태용,윤효인 충남대학교 수의과대학 동물의과학연구소 2003 動物醫科學硏究誌 Vol.11 No.-

To evaluate the clinical efficacy of ciprofloxacin (CFX) on treatment of the pigs with respiratory diseases or diarrhea, CFX at various dosages were administered intramuscularly to the diseased pigs selected from the pig farms located in Chungnam province. One hundreds and fourteen heads of pigs with respiratory diseases were divided into five groups and CFX at 0.25, 0.5, 1.0 ( = 2.5mg CFX/Kg B.W.) and 2.0 doses were administered i.m. for 5 days. The recovery rates were found 31.8%, 73.9%, 91.3% and 92.0%, respectively, being higher than that of the untreated control group (19.0%). The mortality of the treated groups were 0%, that is lower than that of the control (9.5%). Seven kinds of bacteria were isolated from the respiratory organs of 86 pigs, and 34 (39.5%) of 86 pigs showed the complicated infection. In analyzing by the pigs infected with a single pathogen as P. multocida, A. pleuropneumonia, Staphylclcoccus spp. or Mycoplasma spp., the recovery rates were 0∼66.6% at 0.25 dose. 66.6∼100% at 0.5 dose and 100% in all of 4 pathogens at 1.0 to 2.0 doses. At 0.25 dosage the pigs infected with A. pleuropneumonia showed the lowest recovery rate. One hundreds and twenty two heads of pigs with diarrhea were divided into five groups and CFX at 0.25, 0.5, 1.0 ( = 2.5mg CFX/Kg B.W.) and 2.0 doses were administered i.m. for 5 days. The recovery rates were 29.1%, 40.0%, 80.0% and 88.0%, respectively. The mortality of the treated groups were 0-8.3%, being much lower than that of the control. Four kinds of bacteria were isolated from the fecal specimens of 94 pigs, and 84 (89.0%) of 94 pigs showed the complicated infection.

멸치에서 분리한 protease의 특성

정용진,이기동,서지형 東國專門大學 1998 金龜論叢 Vol.6 No.1

최적 반응온도 및 pH에 대한 반응표면분석 결과 멸치 protease 활성의 R^(2)는 0.9237이었으며 5% 이내의 유의수준에서 유의성이 인정되었다. 멸치에서 분리된 protease는 pH 8.03, 반응온도 46.02℃에서 활성이 가장 높았다. Protease 활성은 반응시간이 길어질수록 활성이 증가하였으나 12시간 이상 경과 시에는 큰 변화가 없었다. K^(+)이온은 1mM의 첨가에서는 protease활성을 약간 저해하였으나 10mM로 첨가량을 높인 경우 protease활성을 증가시켰으며, Mg^(2+)이온도 protease활성을 증가시키는 것으로 나타났다. 특히 Cu^(2+)이온은 10mM의 첨가시 protease활성이 3배 이상 증가하였다. NaCl은 첨가량이 증가할수록 protease 활성이 저해 받는 것으로 나타났으며, 25% 이상 첨가시에는 protease활성이 거의 실활 되었다.

Ciprofloxacin의 돼지 호흡기 감염증 및 설사증에 대한 치료효과

전무형,김지영,채미경,서상희,김명철,박창식,김태용,윤효인 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

To evaluate the clinical efficacy of ciprofloxacin (CFX) on treatment of the pigs with respiratory diseases or diarrhea. CFX at various dosages were administered intramuscularly to the diseased pigs selected from the pig farms located in Chungnam province. One hundreds and fourteen heads of pigs with respiratory diseases were divided into five groups and CFX at 0.25, 0.5, 1.0 (= 2.5mg CFX/Kg B.W.) and 2.0 doses were administered i.m. for 5 days. The recovery rates were found 31.8%, 73.9%, 91.3% and 92.0%, respectively, being higher than that of the untreated control group (19.0%). The mortality of the treated groups were 0%, that is lower than that of the control (9.5%). Seven kinds of bacteria were isolated from the respiratory organs of 86 pigs, and 34 (39.5%) of 86 pigs showed the complicated infection. In analyzing by the pigs infected with a single pathogen as P. multocida; A. pleuropneumonia. Staphyloccus spp. or Mymplasma spp., the recovery rates were 0∼66.6% at 0.25 dose, 66.6∼100% at 0.5 dose and 100% in all of 4 pathogens at 1.0 to 2.0 doses. At 0.25 dosage the pigs infected with A. pleuropneumonia showed the lowest recovery rate. One hundreds and twenty two heads of pigs with diarrhea were divided into five groups and CFX at 0.25, 0.5, 1.0 (= 2.5mg CFX/Kg B.W.) and 2.0 doses were administered i.m. for 5 days. The recovery rates were 29.1 %, 40.0%, 80.0% and 88.0%, respectively. The mortality of the treated groups were 0∼8.3%, being much lower than that of the control. Four kinds of bacteria were isolated from the fecal specimens of 94 pigs, and 84 (89.0%) of 94 pigs showed the complicated infection.

심한 척추후측만증 환자의 대퇴골절 복원술을 위한 부위마취 : 증례보고 A case report

김지은,박대용,유시현,강규식,권진형,김천숙,안기량 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Kyphoscoliosis is a disease manifested as lateral curvature of spine and accompanying rotation of the vertebrae, resulting in distortion of rib cage. Restrictive lung disease and pulmonary hypertension progressing to cor pulmonale are the major cause of mortality in patients with kyphoscoliosis. We had experienced a successful spinal and caudal epidural anesthesia for the open reduction and internal fixation with plate of fractured femur shaft in two patients with severe kyphosoliosis. We had chosen regional anesthesia as an anesthetic method because of there poor pulmonary function and severe deformities of thoracolumbar spine. We could achieve the adequate anesthetic sensory dermatome level, T_(5) and T_(8), for operation without any significant deterioration of blood pressure and respiration. We recommended that regional anesthesia is a useful anesthetic method for a patients with severe kyphoscoliosis when the operation on hip or lower extremity is required.

일부 도시농촌 영유아의 빈혈에 관한 연구

강지용,위자형,구연철 梨花女子大學校 醫科大學 醫科學硏究所 1982 EMJ (Ewha medical journal) Vol.5 No.4

From August to September of 1982, the study was conducted a survey of 152 urban infants and children (who visited the well baby and out patient clinic of th Department of Pediatrics of the Han Kang Sung Shim Hospital located at Young Deung Po Koo, Seoul) and of 62 rural infants and children (who are the children and infants living at Soo Dong Myun, Yang Choo Country, which is a comprehensive rural medical care demonstration area of Ewha Womans Universi-ty). The purpose of this survey has been to know the relationship between ane-mia and social demographic background, maternal child health status, and the intake of nutrition. The results were as follows : 1) The frequency of anemia in urban area was shown as 25.7% and that of rural area as 11.7%, which means that the frequency of anemia is higher in ur-ban area than in rural area. 2) In terms of the frequency of anemia according to age, the infants and chil-dren aged 12-18 months in ruban area showed a highest rate of 31.6%, and those aged 0-12 months in rural area a highest rate of 20.0%. In terms of sex, female infants and children were contracted with a little more anemia than male counterparts in urban area. And male infants and children were contracted with a little more anemia than female counterparts in rural area. 3) In terms of mothers, 29 year old mother were set as standard age and those under 29 years in urban area showed an frequency rate of 28.7%, and those un-der 29 years in rural area showed an frequency rate of 13.2%. This means that there was more frequency rate in the mothers under 29 years than in those above 29 years. 4) In terms of economic situation, 290,000 won per month was set as standard amound made per month. And those earning less than 290,000 won in urban areas showed an frequency rate of 35.7%, and those earning less than 290,000 won in rural area 33.3%, which means that there was more frequency of anemia among those making less than 290,000 won than those making more than 290,000 won. And this was significant in terms of statistics. 5) The more the number of siblings was, the higher the frequency of anemia was. The frequency in case of two brothers was shown as 27.6% in ruban area, and as 15.2% in rural area respectively. 6) Such things as number of parity and birth interval were not so much signi-ficant in ruban area. However, the higher the number of parity was in rural area, the higher the frequency of anemia was. And in case birth interval was long, the frequency of anemia was rather high. 7) In terms of the relationship between pregnancy and anemia symptom during pregnancy of index child those pregnant women with anemia symptom in urban area showed an frequency rate of 27.8%, and those in rural area 19.2% respecit-vely. This reveals that the frequency rate of anemia symptom of pregnant women was higher in ruban area than in rural area. 8) In case infant and children took an initial(first) step within the age under one year, those in urban area showed an frequency rate of anemia of 31.7%, which is higher than in those who took an initial step when they were above one year old. And none of the rural infant and children under the age less than one year showed any frequency rate of anemia. 9) In terms of nurition, the infant and children raised by breast feeding showed an frequency rate of anemia of 36.9% and 12.8% in urban area and rural area and rural area respectively. This means that the infant and children who were weaned after six months showed more frequency rate of anemia than those who were weaned within six months. 10) In case food intake was irregular, the frequency rate of anemia were disc-losed as 30.0% and 18.0% un ruban area and rural area respecitvely, which me-ans that irregular food intake has brought about more frequency rate of anemia than regular food intake. And in case iron contained food was rarely taken, the frequency rates of anemia were 25.0% and 3.6% in urban area and rural area respectively. This shows that rarely taking of iron conained food has led to more frequency rate of anemia than frequently taking of iron contained food.

Brucella abortus 국내 분리주의 Heat Shock Protein 암호 groE 유전자의 염기서열 분석과 발현

김태용,김지영,장경수,김명철,박창식,한홍율,전무형 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains. In comparison of the sequences of nucleotides and amino acids among the strains, GroES showed 100% identity in both sequences. GroEL was evaluated 99.0∼99.9% in nucleotides and 98.0∼100% homology in amino acids. The groE gene including groES and groEL was inserted into pET29a vector and constructed pET29a-GroE recombinant plasmids. The inserted groE was confirmed by digestion with Ncol and EcoRl endonucleases and nucleotide sequencing. E. coli BL (DE3) was transformed with pET29a-GroE, named as E. coli BL (DE3)/pET29a-GroE. In SDS-PAGE, it was evident that the recombinant plasmid effectively expressed the polypeptides for GroES (10 kDa) and GroEL (60 kDa) in 0.5, and 2 hours after IPTG induction. The immuno-reactivity of the expressed proteins were proved in mouse inoculation and Western blot analysis.

코자 정(로자탄 칼륨 50mg)에 대한 로자르탄 정의 생물학적 동등성

김용원,박완수,김성수,서지형,조성희,이헌우,류재환,이경태 한국약제학회 2006 Journal of Pharmaceutical Investigation Vol.36 No.2

바닥공조시스템과 천장공조시스템의 실내환경 비교 평가

유기형,조동우,유지용,이진영,이상엽 대한건축학회 2004 대한건축학회논문집 Vol.20 No.5

The purpose of this study was to evaluate the indoor environment of office building with underfloor air distribution system and ceiling based air conditioning system. The underfloor air distribution system was introduced to use access floor and aim to economical motive such as reducing floor to height level. Researches were not sufficient to preserve the characteristics between ceiling based air conditioning system and underfloor air distribution system. Experiments have been carried out to indoor environment in the summer and winter. As the result, thermal environment of the underfloor air distribution system is better than ceiling based air conditioning system in the winter. Ventilation effectiveness of the underfloor air distribution system is bigger than ceiling based air conditioning system.

메밀묵의 제조조건에 따른 텍스쳐 및 관능적 특성 변화

정용진,이명희,서지형,이기동 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.2

Response surface methodology (RSM) was used for optimizing preparation conditions and monitoring the quality of buckwheat mook prepared using buckwheat starch. The textures(hardness, adhesiveness cohesiveness and gumminess) of buckwheat mook were decreased in inverse proportion to the increase of water content. The L and b values of Hunter color parameters in buckwheat mook were increased in proportion to the increase of water content. However, The a value of Hunter color parameters of buckwheat mook were decreased in inverse proportion to the increase of water content. Organoleptic properties(color, form, taste and mouth-feel) of buckwheat mook showed a maximum score in 700㎖(water content), 14min(gelatinization time).

5인 미만 사업장의 산업안전 규제 순응 실태

이관형,이경용,손두익,서남규,서광국,오지영 한국안전학회 2003 한국안전학회지 Vol.18 No.4

This study is planned to investigate the compliance of small sized enterprises of under 5 employees directed by Industrial Safety and Health Act. Occupational safety regulation can be required for employer and employee to comply because that all members of workplace should be involved to protect worker's health. When all members of workplace comply with safety regulation, effectiveness of regulation will be realized as expectation. The study subject is composed of three groups as 501 employers, 501 employees of small sized enterprises of under 5 employees and 107 safety inspectors sampled by stratified random sampling for comparison. Data for analysis is collected from each sample using interview with structured questionnaires. Compliance is measured by 5 point scale composed by 8 sub items such as general perception, understanding, clearness, necessity, relevancy, implementation, penalty, and general compliance of the regulation. Among sub items of employer's compliance, the level of perception of regulation, necessity of regulation, relevancy of penalty are differentiated among three groups. The level of all 8 items of employee's compliance also differentiated among three groups. These results show that strategies for ensuring the same level of compliance among three groups will be needed through education, training and evaluation of regulation and feedback.