포플라 Callus의 배양환경에 따른 Anthocyanin 생합성

방혜진,이승우,최근원,정인식,설일환 경희대학교 생명자원과학연구원 1997 遺傳工學論文集 Vol.9 No.-

Studies were carried out to investigate the effects of various cultural and environmental conditions on callus growth and anthocyanin biosynthesis of the callus derived from the leaf discs of the Populus hybrid (Populus nigra × Populus maximowiczii cv. Yanghwangchul). Even though the largest amount of anthocyanin was obtained from the calli grown in the vessels illuminated with white fluorescent light, no significant differences in callus growth could be obtained among other vessels illuminated with different light qualities, i.e., red, yellow, blue, green and dark. Callus growth and anthocyanin biosynthesis were promoted by long-day conditions (16-hour photoperiod or longer). The calli grown in Schenk & Hildebrandt (SH) medium continuously (SH→SH) showed the best growth whereas the highest amount of anthocyanin biosynthesis was obtained in the calli grown in SH→SH or SH→White media.

한국산 무릇(Scilla scilloides complex)의 세포유전학적 연구 : Ⅱ. 제주도 집단에서 게놈의 분포 Ⅱ. Distribution of Genomes in Chejudo Populations

方在旭,崔慧雲 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Cytogenetic analyses were done in thirteen natural populations of Scilla scilloides complex collected from Chejudo. Six genotypes, BB (2n=18), ABB (2n=26), AABB (2n=34), ABBB (2n=35), AABBB (2n=43) and AAABBB (2n=51) were observed, where A and B denote genomes with χ=8 and χ=9, respectively. Genotypes BB, ABB and AAABBB were found for the first time in Chejudo populations. Genotypes BB and ABB were never reported from the Korean populations. Six of the populations investigated were mixed populations of two or three cytogenetic types, and others were pure populations consisting of BB (1), AABB (4) and AABBB (2). AABB genotypes were predominant over other genotypes and AABBB was the next. AA genotypes growing in the mainland of Korea was not found. It was suggested that the Chejudo populations of S. scilloides complex were closely related to the Japanese populations than mainland populations of Korea. The number of B-chromosome ranged from one to four. The B-chromosomes were all isochromosomes (F) and no chromosome fragment (f) was found. The frequencies of B-chromosome were 75% in BB and AABBB genotypes, respectively. The frequency of B-chromosome in AABB plants was similar to that of AABB plants reported in the mainland populations.

순무(Brassica campestris ssp. rapa) 뿌리로부터 지질화합물의 분리 및 동정

방면호,오영준,유종수,한민우,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H_(2)O분획으로 나누었으며, EtOAc분획에 대해 silica gel column chromatography를 실시하여 3종의 지질화합물을 분리 정제하였다. ^(1)H-NMR, ^(13)C-NMR, DEPT spectrum 및 Mass spectrum등을 통하여 palmitic acid methyl ester(compound 1), linolenic acid methyl ester(compound 2), linoleic acid methyl ester(compound 3)으로 구조를 결정하였다. In order to isolate and identify the active compound from the Brassica campestris ssp rapa, the roots and the root skin were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel column chromatographies. From the result of spectral data including NMR and MS, the chemical structures of the compound were determined as palmitic acid methyl ester (compound 1), linolenic acid methyl ester (compound 2), linoleic acid methyl ester (compound 3).

한국 무릇(Scilla scilloides Complex)의 세포유전학적 연구 : Ⅲ. BB 게놈의 핵형과 B염색체 조성 Ⅲ. Karyotype of Cytotype BB and B-Chromosome Composition

方在旭,崔惠雲 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

A karyotype of cytotype BB plant in Scilla Complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides Complex. The frequency of B-chromosome was 78.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two types of B-chromosomes, F and F', were found; F is a large iso-chromosome and F' a small one.

호밀 (Secale cereale L.) Esterase 동위효소의 유전적 연구

방재욱,최혜운 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, where as EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.

동국의대에서 실시한 2002년 전염병관리실무과정에 대한 평가

방미란,임현술,정해관,한영란,정철 대한보건협회 2003 대한보건연구 Vol.29 No.1

Communicable diseases have recently reemerged, with new communicable diseases continually breakmg out on a global scale; threaten the health and economics of populations. The Korean National Institute of Health carries out Field Management Training Prcgrams (FMTP) to address this problem The objectives of this study were the evaluation of the understanding and satisfaction for this program, and recommendation for improvement strategies. We conducted a questionnaire survey of 80 persons who completed the FMTP, at Dongguk University, between March 1 and November 30, 2002. The data collected was analyzed by Student s t-statistics. The understanding of the curriculum was Well in 42 (52.5%) and Moderate in 24 persons (30.0%). The satisfaction with the curriculum was Moderate in 38 (47.5%) and Good in 30 persons (37.5%). The satisfaction for the period of education was Bad for 31 persons (38.7%), which accounted for the majority of cases. The mean scores, by Likert s scale, for the understanding and satisfaction of the curriculum were 3.70 (±0.71) and 3.32 (i0.721, respectively. The mean score for the epidemiological survey was the highest for satisfaction with the course, at 3.78 (±0.62). The mean value of satisfaction for curriculum in education of period was significantly higher in health department group than in nursing department group (P<0.05). From this survey it appears the respondents were satisfied with this program. The FMTP will be systematically revised for curriculums, and effort will be required to develop controls for communicable disease, both by the government and the university.

수용액상의 Fe(EDTA) 착물에 의한 NO 가스의 흡착에 관한 열역학적 연구

방효춘,김성옥,정해영 德成女子大學校 1996 德成女大論文集 Vol.27 No.-

In order to develope the method to remove the water-insoluble pollutant NO? in aqueous solution, metal chelate complex was used. Among the series of metals and chelates, Fe?(EDTA) was the first choice. On this paper, the binding of NO gas to Fe?(EDTA) was monitored through GC and thermodynamic parameters of that reaction was obtained. The equilibrium constants for the the binding of NO gas to Fe?(EDTA) are 1.44×10?M? at 40℃, 2.49×10?M? at 20℃, also△H?=-10.81㎉/㏖ and △S?=-12.00㎈/㏖·K.

집토끼 동방결절의 부위별 활동전압에 미치는 Vanadate의 효과

김해수,방효원,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.1

In order to investigate the effect of vanadate on the action potentials of the rabbit S-A node, 3M KC1 glass microelectrodes were introduced and analyzed in the central, transitional and peripheral areas in various strips which were prepared from a whole rabbit S-A node. The followings were results of findings: 1. In cases of vanadate 320μM/ℓ treatment, spontaneous firing rates were decreased to 78.7±6.94, 78.5±35.5 and 64..5±7.42% of the centrals respectively in the central, transitional and peripheral areas. 2. Maximum diastolic potentials(M.D.P.) were slightly increased in all three areas. 3. Overshoot potentials were increased at the central and peripheral areas but decreased at the transitional area in the vanadate-treated group. 4. Maximum depolarization rate(Vmax) were increased in all three areas. 5. Changes of the action potential induced by vanadate were qualitatively similar to those occuring when the calcium concentration of the bathing medium was raised to 4mM. These results may be interpreted as that vanadate increase the rate of Ca^2+ influx into all the cells in the S-A node.

제조업의 경쟁력과 재무성과와의 인과모형에 관한 실증적 분석

박해철,방재일 中央大學校經營硏究所 2002 經營學論集 Vol.28 No.2

탄화수소 자화세균의 분리

宋邦鎬,河惠貞 慶北大學校 1989 論文集 Vol.47 No.-

Four strains of hydrocarbon utilizing bacteria from the sludges of petroleum factory were isolated by testing the growth abilities to utilize methanol, benzene, paraffin and petroleum. Each strain was designated as KM5001 ,5002 ,5003 and 5004, and the putative genus was identified as Neisseria, Flavobacterium, Streptococcus, and Pseuomonas, respectively. These strains were grown well in the CⅢ minimal medium supplemented with above hydrocarbon sources. The strains KM-5001 and 5002 were grown better than the others by utilizing various hydrocarbons and methanol. However, considering its doubling time and specific growth rates, all strains could be applicable for the industrial use for the purpose of removing the petroleum contamination from the sea and river. The strains were grown well from pH 6.0 to 7.0, while the growth rate was decreased at pH 9.0. The optimal growth temperature was 40C. Above40C, the growth rate of cells reduced extremely. All strains carried plasmids in which the genes for utilizing hydrocarbons may be inherent.