쪽의 핵형분석과 rRNA 유전자의 염색체상 위치

최혜운,이상훈,김수영,방재욱 한국약용작물학회 2008 한국약용작물학회지 Vol.16 No.3

Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 μm to 1.50 μm in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

Angelica속 식물 7종의 세포유전학적 분석

최혜운,구달회,이우규,김수영,성정숙,성낙술,서영배,방재욱 韓國藥用作物學會 2005 한국약용작물학회지 Vol.13 No.3

한국에서 재배되고 있는 당귀속 (Angelica) 식물 7종을 대상으로 핵형 분석을 수행하여 다음과 같은 결과를 얻었다. 당귀속 식물들의 기본염색체 수는 x=11로, 체세포 염색체 수는 모든 종에서 2n = 2x = 22로 관찰되었다. 참당귀의 핵형은 K(2n) = 2x = 20m + 2sm, 일당귀의 핵형은 K(2n) = 2x = 12m + 10sm, 당당귀의 핵형은 K(2n) = 2x =16m + 6sm, 고본의 핵형은 K(2n) = 2x = 22m, 바디나물은 K(2n) = 2x = 18m + 4sm, 구릿대는 K(2n)= 2x = 10m + 10sm 2st, 왜친궁은 K(2n)= 2x = 22t로 각각 구분되었다. 염색체 크기는 3.56 μM-8.91 μM 사이였다. 고본의 체세포 염색체는 모두 중부 염색체로, 왜천궁은 모두 차단부 염색체로만 관찰되어 다른 종들과 이질적인 핵형을 보였다. 한국에 본포하고 있는 당귀속 식물의 핵형과 러시아, 일본, 중국에 분포하는 식물들의 핵형과 비교에서는 일부 다형현상이 관찰되었다. Karyotypes were established in seven Angelica species cultivated in Korea. The somatic chromosome numbers were 2n = 2x = 22 with the basic number of x = 11 in all Angelica plants examined. Their metaphase chromosomes ranged from 3.56 μm. to 8.91 x. in length. Distinctive Karyotypes were found in two species, A. tenuissima with all metacentries, K(2n) = 2x = 22m, and A. genuflexa with all subtelocentrics, K(2n) = 2x = 22st. Karyotype formulas of A. gigas, A. acutiloha, A. sinensis, A. decursiva and A. dahurica were K(2n) = 2x = 20m + 2sm, K(2n) = 2x = 12m + 10sm, K(2n) = 2x = 16m + 6sm, K(2n) = 2x = 18m + 4sm and K(2n) = 2x = 10m + 10sm + 2st, respectively. Cytological data showed that chromosomal polymorphisms within species were observed in Angelica plants compare to other regions.

한국 무릇 ( Scilla scilloides Complex ) 의 세포유전학적 연구 - 1. 게놈에 따른 분포 및 B 염색체의 조성과 출현 빈도

최혜운(Hae Woon Choi),방재욱(Jae Wook Bang) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.4

Geographical distribution of diploid plants with AA genome (2n=16)and allotetraploid with AABB genome (2n=34) of Scilla scilloides Complex from Korea has been studied. The composition and frequencies of B chromosomes were also investigated. Plants with AABB genome were predominant over AA genome plants. A mixed population of AA and AABB genome plants was found for the first time. Aneuploid plants have not been found. Chromosomes of AA genome were composed of three pairs of metacentric, two pairs of submetacentric, two pairs of subtelocentric and one pair of telocentric chromosomes, whereas BB genome was four pairs of metacentric and five pairs of subtelocentric chromosomes. B chromosomes were classified into two categories, isochromosome (F) and chromosome fragment (f). The frequencies of B chromosomes were 43% in AA genome plants and 44% in AABB genome plants. The number of B chromosome ranged from 1 to 3 and 1 to 7 in AA and AABB genome plants, respectively. B chromosome combinations were F and F+f in AA genome plants and F,F+f and f in AABB genome plants.

호밀 (Secale cereale L.) Esterase 동위효소의 유전적 연구

방재욱,최혜운 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, where as EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.

게놈 유형이 다른 무릇(Scilla scilloides comlpex)에서 FISH를 이용한 인 우성 다양성 분석

이상은,최혜운,방재욱 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2

Nucleolar dominance, an epigenetic phenomenon in which one parental set of ribosomal RNA gene is silenced in an interspecific hybrid, was investigated in different cytotype plants of Scilla scilloides complex; AA, BB, AABB, ABBB, BBBB and AABBB. To determine the loci of the nucleolar organizer regions (NORs) in metaphase chromosomes, physical mapping of 45S rRNA gene (3.8 Kb) using FISH method was applied. Two signals of the 45S rRNA gene loci were observed in both the cytotypes of AA (2n=16) and BB (2n=18). Four signals were found in tetraploid BBBB, while two and three signals were found in cytotypes of AABB and ABBB, respectively. However, AABB plants in the population of Daegukhul-do Island had four NORs in both the A and B genome. Four signals were found in pentaploid AABBB plants. The different numbers of 45S rRNA gene signals suggested the phenomenon of nucleolar dominance or amphiplasty among different cytotype plants of S. scilloides. It is speculated that inactivation of NORs on a2 chromosome in the cytotype AABB plants is the result of the amphiplastic suppression between A and B genomes.

수영 ( Rumex acetosa ) 의염색체 연구 CHROMOSOME STUDIES ON Rumex acetosa

이미경,최혜운,방재욱 한국유전학회 1990 Genes & Genomics Vol.12 No.4

N/A

전기영동법에 의한 호밀 ( Secale cereale L . seedling proteins 의 분석

방재욱,최혜운 한국유전학회 1987 Genes & Genomics Vol.9 No.4

N/A

지모에서 McFISH를 이용한 rDNAs의 물리지도 작성

김수영,최혜운,방재욱 韓國藥用作物學會 2004 한국약용작물학회지 Vol.12 No.6

약용식물로 재배되고 있는 지모를 대상으로 McFISH 기법을 이용하여 45S와 5S rDNA 유전자의 염색체상의 위치를 확인하여 물리지도를 확립하였다. 2쌍의 45S rDNA는 1번 염색체의 단완 말단과 3번 염색체의 동원체 부위에서 관찰되었고, 1번 염색체의 signal이 3번 염색체에서의 signal보다 더 강하게 나타났다. 한 쌍의 5S rDNA signal은 45S rDNA signal과 함께 3번 염색체의 동원체 부위에서 관찰되었다. Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.

할미꽃속 식물 5종의 핵형 분석

이우규,최혜운,방재욱 한국약용작물학회 2004 한국약용작물학회지 Vol.12 No.6

한국에 분포하고 있는 할미꽃속 5종에 대한 핵형 분석을 수행하여 다음과 같은 결과를 얻었다. 체세포 중기 염색체 수는 모두 2n=2x=16, 기본 염색체 수는 x=8로 관찰되었다. 가는잎할미꽃 (P. cernua)의 염색체 조성은 5쌍의 중부 염색체 (1, 2, 3, 4, 5번), 1쌍의 차중부 염색체 (6번)와 2쌍의 차단부 염색체 (7, 8번)로 핵형은 K(2n) = 2x = 16 = 10m + 2sm + 4st로 나타났고, 염색체의 크기는 4.62~8.25 μm였다. 분홍할미꽃 (P. davurica), 할미꽃 (P. koreana) 및 중국할미꽃 (P. chinensis) 염색체 조성은 5쌍의 중부 염색체 (1, 2, 3, 4, 5번)와 3쌍의 차단부 염색체 (6, 7, 8번)로 핵형은 K(2n) = 2x = 16 = 10m + 6st로 유사하게 나타났다. 염색체의 크기는 분홍할미꽃 에서 5.90~10.66 μm, 할미꽃에서 5.25~8.80 μm, 중국할미꽃에서 4.33~6.99 μm로 차이를 보였다. 동강할미꽃 (P. tongkangenesis)의 염색체조성은 5쌍의 중부 염색체 (1, 2, 3, 4, 5번), 2쌍의 차중부 염색체 (6, 7번)와 1쌍의 차단부 염색체 (8번)로 핵형은 K(2n) = 2x = 16 = 10m + 4sm + 2st로 구분되었으며, 염색체의 크기는 4.67~8.97 μm로 나타났다. Karyotypes were established in five Pulsatilla species from Korea : P. cernua, P. davurica, P. koreana, P. chinensis and P. tongkangensis. The somatic chromosome numbers of five species were all 2n=2x=16 with the basic number of x=8. The chromosome complement of P. cernua consisted of 5 pairs of metacentric, 1 pair of submetacentric and 2 pairs of subtelocentric. P. davurica, P. koreana and P. chinensis consisted of 5 pairs of metacentric and 3 pairs of subtelocentric. P. tongkangensis consisted of 5 pairs of metacentric, 2 pairs of submetacentric, and 1 pair of subtelocentric. Karyotype formulas of P. davurica, P. koreana, and P. chinensis were the same as K (2n) = 2x = 16 = 10m + 6st, while those of P. cernua was K (2n) = 2x = 16 = 10m + 2sm + 4st and P. tongkangensis was K (2n) = 2x = 16 = 10m + 4sm + 2st, respectively.

깽깽이풀의 핵형분석과 McFISH를 이용한 rDNA의 물리지도 작성

김수영,최혜운,구달회,김찬수,방재욱 韓國藥用作物學會 2005 한국약용작물학회지 Vol.13 No.1

보호식물이며, 약용식물인 깽깽이풀 (Jeffersonia dubia)을 대상으로 핵형 분석과 McFISH 기법을 이용한 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 체세포 염색체 수는 2n=2x=12였으며, 2쌍의 중부 염색체 (염색체 1, 3), 2쌍의 차중부 염색체 (염색체 2, 4) 그리고 2쌍의 차단부 염색체 (염색체 5, 6)로 구분되었고, 염색체의 평균 길이는 1.95~3.50μM이었다. McFISH기법을 이용하여 45S와 5S rDNA의 염색체상의 위치를 확인한 바, 3쌍의 45S rDNA signal은 4번, 5번 그리고 6번 염색체의 단완 말단 부위에서 관찰되었고, 한 쌍의 5S rDNA signal은 2번 염색체의 동원체 부위에서 관찰되었다. Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged 1.95~3.50 μm. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.