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          The effect of positive-end expiratory pressure on oxygenation during high frequency jet ventilation and conventional mechanical ventilation in the rabbit model of acute lung injury

          방재욱,하승일,최인철 대한마취통증의학회 2012 Korean Journal of Anesthesiology Vol.63 No.4

          Background: The use of positive end expiratory pressure (PEEP) in patients with acute lung injury (ALI) improves arterial oxygenation by alleviating pulmonary shunting, helping the respiratory muscles to decrease the work of breathing, decreasing the rate of infiltrated and atelectatic tissues, and increasing functional residual capacity. In a rabbit model of saline lavage-induced ALI, we examined the effects of PEEP on gas exchange, hemodynamics, and oxygenation during high frequency jet ventilation (HFJV), and then compared these parameters with those during conventional mechanical ventilation (CMV). Methods: Twelve rabbits underwent repeated saline lavage to create ALI. The animals were divided in 2 groups: 1) Group CMV (n = 6), and 2) Group HFJV (n = 6). In both groups, we applied 2 levels of PEEP (5 cmH2O and 10 cmH2O) and then measured the arterial blood gas, mixed venous blood gas, and hemodynamic parameters. Results: With administration of PEEP of either 5 cmH2O or 10 cmH2O, the arterial oxygen content of both groups was increased, although without statistically significant differences between groups. On the contrary, the arterial carbon dioxide content was significantly decreased in the HFJV group, as compared with the CMV group, during the entire experiment. Furthermore, there was significant decreases in mean arterial pressures in both groups with a PEEP of 10 cmH2O. Conclusions: The application of PEEP in rabbits with ALI effectively improves oxygenation in either HFJV or CMV. Background: The use of positive end expiratory pressure (PEEP) in patients with acute lung injury (ALI) improves arterial oxygenation by alleviating pulmonary shunting, helping the respiratory muscles to decrease the work of breathing, decreasing the rate of infiltrated and atelectatic tissues, and increasing functional residual capacity. In a rabbit model of saline lavage-induced ALI, we examined the effects of PEEP on gas exchange, hemodynamics, and oxygenation during high frequency jet ventilation (HFJV), and then compared these parameters with those during conventional mechanical ventilation (CMV). Methods: Twelve rabbits underwent repeated saline lavage to create ALI. The animals were divided in 2 groups: 1) Group CMV (n = 6), and 2) Group HFJV (n = 6). In both groups, we applied 2 levels of PEEP (5 cmH2O and 10 cmH2O) and then measured the arterial blood gas, mixed venous blood gas, and hemodynamic parameters. Results: With administration of PEEP of either 5 cmH2O or 10 cmH2O, the arterial oxygen content of both groups was increased, although without statistically significant differences between groups. On the contrary, the arterial carbon dioxide content was significantly decreased in the HFJV group, as compared with the CMV group, during the entire experiment. Furthermore, there was significant decreases in mean arterial pressures in both groups with a PEEP of 10 cmH2O. Conclusions: The application of PEEP in rabbits with ALI effectively improves oxygenation in either HFJV or CMV.

        • SCOPUSKCI등재

          호밀 염색체 C - banding pattern 의 다형현상

          방재욱,이웅직 한국유전학회 1984 Genes & Genomics Vol.6 No.3

          한국에서 생육되고 있는 호밀 중 5개 지방산 호밀에서 염색체의 C-banding pattern의 다형현상을 분석하였다. 다형현상은 다음과 같이 나타났다. 염색체I : 장완 중앙의 band, 장완의 subterminal band 염색체II : 다형현상 없음. 염색체III : 장완 말단의 band 염색체IV : 장완 중앙의 band 염색체V : 장완과 단완의 intercalary band 염색체VI : 장완의 intercalary band 염색체VII : 장완 중앙의 band B염색체에서는 영월산 호밀에서 2개의 intercalary bands가 나타나지 않는 것이 특징이었다.

        • SCOPUSKCI등재

          호밀 Seedling Protein 의 전기영동 Pattern

          방재욱 한국유전학회 1986 Genes & Genomics Vol.8 No.1

          한국에서 생육되고 있는 호밀 계통을 재료로 하여 seedling proteins의 SDS-PAGE(sodiumdodecylsulfate-polyacrylamide gel electrophoresis) pattern과 2-D 전기영동(two-dimensional electrophoresis) pattern을 조사하였다. SDS-PAGE pattern에 있어서 계통별로 band의 수와 크기의 차이를 나타냈으며, 발아 시간별로는 band의 크기에 있어서의 차이를 관찰할 수 있었다. Seedling의 부위별로는 뿌리와 자엽이 비슷한 band pattern을 나타냈으며, seed와는 많은 차이를 보였다. 2-D pattern에서 80여개의 polypeptides가 비교 가능하였다. 전체적인 양상은 계통간에 비슷하게 나타났으며, Type 1(position difference)보다는 Type 2(presence/absence)와 Type 3(intensity difference)의 차이가 많이 관찰되었다 B염색체의 존재유무에 따른 차이도 마찬가지였다.

        • SCOPUSKCI등재

          전기영동법에 의한 호밀(Secale cereale L .seedling proteins)의 분석

          방재욱,최혜운 한국유전학회 1987 Genes & Genomics Vol.9 No.4

          금산 호밀 (Secale cereale L.)의 seedling을 재료로 Polyacrylamide Gel Electrophoresis (PAGE)를 이용하여 호밀의 계통, seedling의 각 기관 및 발달단계에 따른 total protein, secalin 및 isozymes의 차이를 분석하였다. SDS-PAGE 분석에서 total protein과 secalin의 pattern이 계통 및 발달단계에 따라 차이를 나타냄을 관찰할 수 있었다. PAGE에 의한 isozymes의 분석에서도 seedling의 기관 및 발달 단계에 따른 isozyme band의 수와 activity의 차이를 볼 수 있었다.

        • SCOPUSKCI등재

          전기영동법을 이용한 호밀(SECALE CEREALE L .)SEEDLING PORTEINS 의 유전적 분석 : SOLUBLE PROTEINS 의 SDS - PAGE 와 2차원 전기영동 SDS - Page of Soluble Proteins and Two - Dimensional Electrophoresis

          방재욱 한국유전학회 1988 Genes & Genomics Vol.10 No.2

          The seedling proteins of rye were investigated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis. Also the difference of electrophoretic pattern related to the presence of B chromosomes was studied. In SDS-PAGE gels, mass differences in high molecular weight proteins were observed between plants with B and without B chromosomes. More than 90 polypeptide spots were scored on 2-D gels. In comparison of 2-D electrophoretic patterns between seedlings with and without B chromosomes, mass modifications were found in 3 polypeptides on gels.

        • SCOPUSKCI등재후보

          한국 무릇(Scilla scilloides Complex)의 세포유전학적 연구 : Ⅲ. BB 게놈의 핵형과 B 염색체 조성 Ⅲ. Karyotype of Cytotype BB and B-Chromosome Composition

          方在旭,崔惠雲 한국식물학회 1993 Journal of Plant Biology Vol.36 No.3

          A karyotype of cytotype BB plant in Scilla scilloides complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides complex. The frequency of B-chromosome was 38.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two types of B-chromosomes, F and F', were found; F is large iso-chromosome and F' a small one.

        • SCOPUSKCI등재후보

          한국산 무릇(Scilla scilloides complex)의 細胞유전학적 연구 : Ⅱ. 제주도 집단에서 게놈의 분포 Ⅱ. Distribution of Genomes in Chejudo Populations

          方在旭,崔慧雲 한국식물학회 1991 Journal of Plant Biology Vol.34 No.2

          Cytogenetic analyses were done in thirteen natural populations of Scilla scilloides complex collected from Chejudo. Six genotypes, BB (2n=18), ABB (2n=26), AABB (2n=34), ABBB (2n=35), AABBB (2n=43) and AAABBB (2n=51) were observed, where A and B denote genomes with x=8 and x=9, respectively. Genotypes BB, ABB and AAABBB were found for the first time in Chejudo populations. Genotypes BB and ABB were never reported from the Korean populations. Six of the populations investigated were mixed populations of two or three cytogenetic types, and others were pure populations consisting of BB (1), AABB (4) and AABBB (2). AABB genotypes were predominant over other genotypes and AABBB was the next. AA genotypes growing in the mainland of Korea was not found. It was suggested that the Chejudo populations of S. scilloides complex were closely related to the Japanese populations than mainland populations of Korea. The number of B-chromosome ranged from one to four. The B-chromosomes were all isochromosomes (F) and no chromosome fragment (f) was found. The frequencies of B-chromosome were 75% in BB and AABBB genotypes, respectively. The frequency of B-chromosome in AABB plants was similar to that of AABB plants reported in the mainland populations.

        • SCOPUSKCI등재후보

          밀(Triticum spp.)의 미성숙배로부터 유도한 현탁 배양세포에서의 염색체 변이

          方在旭 한국식물학회 1990 Journal of Plant Biology Vol.33 No.3

          Suspension cell lines have been newly established from the calli derived from the immature embryo culture of hexapolid(Triticum aestivum var.sicco), tetrapolid(T.durum) and diploid (T. tauchii or Aegilops squarrosa) wheat species. The chromosomal variation in suspension cultured cell lines was examined and old cell line, C82d, established from T. aestivum var. copain was also used. New method using 1-bromonaphthalene for metaphase trapping of suspension cells was developed. Variation in chromosome number was observed amongst all the suspension lines. Cells with doubled chromosome number and deleted chromosome were also observed. Extensive structural changes in chromosomes were found in C82d line. Chromosome aberrations showed loss of chromosome arms and chromosome segment. The mean chromosome number in suspension cells of T. aestivum var. sicco was 40, in C82d line 33, in T.durum 28 and in T. tauchii 14. The stability of chromosomes in suspension cells of diploid and tetrapolid wheats was higher than that of hexaploid wheat.

        • SCOPUSKCI등재후보

          韓國 호밀(Scale cereale L.)의 B 染色體 出現密度와 地理的 分布

          方在旭,李雄稙 한국식물학회 1986 Journal of Plant Biology Vol.29 No.2

          The frequencies and geographical distribution of B chromosomes on 15 strains of rye(Secale cereale L.) collected from various localities in Korea were investigated. All of the 15 strains of rye investigated were found to have B chromosomes, and the frequencies of B chromosomes ranged from 6% to 51% with 20.1% average. Plants with 2Bs seem to be the most stable in populations with B chromosomes. Of 1400 plants examined, one plant was observed to have a deficient-B chromosome in Buyo rye.

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