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호밀(Secale cereale L .)Esterase 동위효소의 유전적 연구
방재욱,최혜운 한국유전학회 1990 Genes & Genomics Vol.12 No.2
Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, whereas EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.
방재욱 한국유전학회 1988 Genes & Genomics Vol.10 No.2
The seedling proteins of rye were investigated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis. Also the difference of electrophoretic pattern related to the presence of B chromosomes was studied. In SDS-PAGE gels, mass differences in high molecular weight proteins were observed between plants with B and without B chromosomes. More than 90 polypeptide spots were scored on 2-D gels. In comparison of 2-D electrophoretic patterns between seedlings with and without B chromosomes, mass modifications were found in 3 polypeptides on gels.
호밀 (Secale cereale L.)B染色體의 Transmission에 관한 硏究
方在旭 木浦大學校基礎科學硏究所 1984 基礎科學硏究誌 Vol.2 No.-
The transmission of B chromosomes in PALDANG rve(Secale cereale L.) was investigated. In the progeny of the cross in population of Bs plants average number of B chromosome per plant increased 2 to 2.5 in first generation. In the progeny of 2Bs×2Bs, the B chromosome number per plant was found to be 2.1 and in the case of the crosses between 4Bs( ♀) and 2Bs (♂), it appeared 3.5, 3.1 and 3.2 respectively.