Caco-2 세포 단층막 투과 실험시 교반이 약물의 투과계수에 미치는 영향

홍순선,유호정,이홍,정석재,김대덕,심창구 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

The unstirred water layer (UWL), which has been known to exist in the boundary of the intestinal lumen and intestinal wall, often behaves as an absorption barrier especially for lipophilic drugs. The intestinal absorption of drugs is often characterized using Caco-2 cell monolayers grown on Transwell polycarbonate membranes. The permeability (Pare) of drugs across the cell monolayer might be influenced by the agitation of the donor compartment, since the width of UWL on the surface of the cell monolayer would be reduced by the agitation. In this study, the effect of agitation of the donor compartment with 60 rpm on the permeability was measured for 12 drugs with a wide range of lipophilicity and permeability. The of mannitol, tributylmethyl ammonium, cimetidine, ranitidine, hydrocortisone, benzylpenicillin and loxoprofen was not influenced by the agitation, while the P_(app) of theophylline, propranolol, YH439, phenylpropanolarnine and testosterone was increased by the agitation. There was a significant correlation between the increase of P_(app) by agitation and the lipophilicity for the compounds having P_(app) > 2 x 10^(-5) cm/sec. No correlation was observed for the difference in P_(app) by agitation and the molecular weight, or lipophilicity of the drugs. Therefore, the agitation rate of the donor compartment in the Caco-2 cell monolayer study should be carefully controlled in order to estimate Pap, reproducibly especially for iipophilic drugs.

더블 팬터그래프형 매니퓰레이터의 설계 및 수평위치제어

홍대선,신중호,정원지,김성철 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

In this paper, a double pantograph type manipulator activated by two slider joints is proposed for a fuel handling machine in atomic power plants. The manipulator consists of two parallelogrammic structures considered as simplified 2-DOF linkage systems. The linkage motion is controlled in such a manner that the joint rates of two linkages are constant and the movement of end-effector is horizontal, because the constant joint rates have advantages for the stable movement of the system. In addition, the static deflection of the proposed mechanism was analyzed using a transfer-stiffness matrix method.

관동맥 재개통 후 QTc간격분산의 감소 : 정량적으로 측정한 심근 허혈의 호전 정도와의 연관성 Relationships with the Quantitative Degree of Improvement in Myocardial Ischemia

박상선,최기준,남기병,이철환,홍멸기,박성욱,박승정,신중우,문대혁,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

국내 데크작업의 생산성 증대에 관한 연구

윤대중,윤준선,백준홍 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The use of plates have gone on constantly increasing the slab system of the steel structures in construction field but the productivity of deck plate installation has constantly declined because of the lack of coordination, fragmented nature, and queuing time. This paper provides models for analyzing the complex cause and effect relationships that may lead to a loss of productivity in construction. The purpose of this study is to suggest the methods to improve the productivity and reduce the idle time of the deck plate installation through work sampling, crew balance, work sequence evaluation, and Extend simulation. The model introduced in the paper will provide a new approach to evaluating occurrences of productivity.

항결핵 치료과정에서 호전되었다가 악화된 뇌 결핵종 1예 보고

이대훈,유진홍,강미자,김홍기,김선화,고동훈,천지성,유원종 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.3

In spite of nationmide tuberculosis control program, tuberculosis is still prevalent in Korea. Accordingly, tuberculous meningitis also poses one of the main trouble some problems. Recently, We experienced a perplexing case of tuberculous menin-gitis and tubercdoma in a 24-year old female patient. She was admitted to our hospital owing to meningeal irritation sign, aphasia, VI nerve palsy, and ,fever. CSF analysis showed a finding consistent with that of presumptive tuberculous meningitis.. Initial MRI of the brain revealed a large mass(tuhercu1oma) on left parieto-temporal area. Antitubeculous regimen(1soniazid (INH), rifampin(RMP), ethambutol(EMB), pyrazinamide(PZA)} of meningeal dose was given immediately, and her symptoms and signs were dramatically disappeared with a few days after treatment. She was discharged with improved condition and under maintenance treatment of tuberculosis. Follow-up one year after treatment. revealed that the previous lesion was nearly diminished. She showed no symptom at all until she recognized a total blindness of sudden onset on her left eye a t the 18th month of treatment. Brain MRI checked at that time showed multiple masses of tuberculoma scattered over the brain base and one of them invaded left optic nerve. In our opinion, the cause of relapse in this patient might he explained by the selection and ultimate replication of a minor population of resistant strain. Although such relapse is an extremely rare event, we think that we should always pay attention to this possible resistance problem every time we meet patients with tuberculous meningitis.

건설 클레임의 예측모델에 관한 연구 : 턴키프로젝트 중심으로

윤대중,윤준선,백준홍 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The purpose of this study is to identify those attributes that affect a construction project, and to construct a model to predict construction claim in Turn-key construction projects. Data for the model development were collected using forty questionnaire surveys. This study developed a model and the likelihood of construction claims was modeled using logistic regression. People, process, and project issues play a role in predicting the likelihood of Turn-key construction claims. In this paper, the theoretical framework for predictable factors is first presented. This is followed by the research methodology. The results of the relevance of the attributes as tested in the fieldwork are then presented and discussed. Next, the Logistic regression model development is described, followed by model validation. The final section discusses the limitations of this paper and concludes the study. The essence of this study is that claims in Turn-key construction projects can be predicted by using variables.

PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

동적 GTM와 BMA을 이용한 움직이는 객체의 얼굴 검출

장혜경,홍종선,강대성 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.11 No.1

This paper estimates dynamic object information in video stream and proposes face detection technique using dynamic GTM(genetic tree-map) algorithm. The existing general object estimate methods divided image information through cluster process, and estimated as reconstructing the moving object of the separate information. On the other hand, proposed method obtains object information that is moving in video stream as taking advantage of BMA(b1ock matching algorithm) and employs PCA(principal component analysis) as cluster algorithm. PCA algorithm searches for principal components utilizing statistical properties for input data, and then divides data making use of region division algorithm. Only face region is detected in specific object abstracted using separate object information, according to dynamic GTM map information generated about feature of face region using dynamic GTM.

리니어 모터로 이송되는 탄성체의 진동 최소화를 위한 이송속도 최적화 연구

최영휴,홍대선,최응영,최국진 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

Linear motor feed drive systems have been broadly used in machine tools or precision automatic systems. Recently, modern machine tools require high speed and high precision feed drive system to achieve high productivity. Unfortunately, a feed drive system, even though it was designed optimally, may experience severe transient vibrations during high-speed operation if its feed rate control is unsuitable. A rough feed rate curve having discontinuity in its acceleration profile causes serious vibrations in the feed slides system. This paper presents a feed rate optimization of a machine tool feed slide system, which is driven by a linear motor, for its minimum vibrations. Firstly, a 4-degree-of-freedom lumped parameter model is proposed for the vibration analysis of a linear motor driven machine tool feed drive system. Next, a feed rate optimization of the feed slide is carried out for minimum vibrations. The feed rate curve optimization strategy is to find out the most appropriate acceleration profile with jerk continuity. Of course, the optimized feed rate should approximate to the desired one as possible. A genetic algorithm with variable penalty function was used in this feed rate optimization.

다량의 하부위장관출혈이 병발된 Vibrio vulnificus 패혈증 1예

최주연,유진홍,한석원,이대훈,최민호,박순민,김연식,김선우,신완식,강문원,강성구 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.2

최근 저자들은 Vibrio vulnificus 감염에 의해 패혈증 및 이차성 피부 병변을 보인 42세의 남자 환자의 치료중 하부 위장관 출혈이 병발된 1예를 경험하였기에 보고하는 바이다. We experienced a case of Vibrop vulnificus septicemia which showed an unusual manifestation such as hematochezia. The patient was a 42-year-old man with chronic liver disease. The blood and wound culture revealed the organism. During intensive care, he suffered from massive hematochezi for several days. Colonoscopy and abdominal CT showed findings suspicious of ischemic colitis. To our knowledge, this is the first unusual case of V.vulnificus infection which showed massive lower gastrointestinal bleeding.