Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

말초혈액에서 Tg mRNA에 대한 역전사 중합효소 연쇄 반응법의 갑상선 재발암의 분자생물학적 진단

권성일,박기룡,김현영,신채희,임영찬,최영식,박요한,이강대,장희경,이재화,염하용 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 갑상선암은 다른 조직에 발생한 암에 비해 비교적 천천히 자라므로 대부분 예후가 양호하지만, 일부에서는 주위 조직으로 침윤하거나 혹은 원격 전이로 인하여 치명적인 결과를 초래할 수 있다. 갑상선전절제술 및 131^I 제거술 후 경과 관찰시 갑상선암의 재발과 전이의 진단에 있어 131^I 스캔과 혈청 Tg의 측정이 현재 임상에서 가장 많이 이용되고 있으나 이 방법에는 여러 가지의 결점이 있다. 최근 Tg mRNA에 대한 RT-PCR법을 이용한 여러 연구결과는 131^I 스캔과 혈청 Tg 측정의 결점을 보완할 수 있는 좋은 보조적인 진단법으로 이용할 수 있을 가능성을 제시하였다. 이에 말초혈액에서 측정한 Tg mRNA에 대한 RT-PCR법이 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무의 진단에 유용한가를 알아보고자 이 연구 시행하였다. 방법: 분화된 갑상선암으로 진단된 후 갑상선전절제술을 시행받고 방사성요드 치료를 받은 환자 중 현재까지 한차례에 이상 추적 방사성요드 전신 스캔을 시행하고 추적관찰이 가능했던 유두선암 35예, 여포선암 5예를 대상으로 연구를 시행하였다. 대상군은 131^I 스캔 소견상 음성인 군(Group Ⅰ), 잔여조직이 있는 군(Group Ⅱ), 국소전이가 있는 군(Group Ⅲ), 및 원격전이 군(Group Ⅳ)으로 구분하였다. 정상 대조군은 갑상선질환이 없는 10예의 건강인으로 하였다. 대상환자의 말초혈액을 이용한 Tg mRNA에 대해 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행하였다. 결과: 본 연구 결과는 다음과 같다. 1) 131^I 스캔 소견상 음성인 군 21예 중 1예에서 Tg가 양성소견을 보였다. Anti Tg Ab가 양성인 4예 모두 Tg가 음성을 보였다. 잔여조직이 있거나 국소전이 및 원격전이를 보인 군 19예 중 Tg가 양성인 경우는 4예였으나, Tg mRNA는 전예에서 양성이었다. 2) 131^I 스캔에서 국소 및 원격전이 소견을 보인 8예 중 4예에서 Tg가 음성으로 131^I 스캔과 혈청 Tg 사이에 불일치 소견을 보였다. 3) 말초혈액에서 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행한 결과 대상군 40예 및 정상 대조군 10예 모두에서 Tg mRNA가 양성을 보였다. 결론: 본 연구에서 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무를 평가함에 있어 역전사 중합효소 연쇄 반응법을 이용한 Tg mRNA 측정의 의의는 재평가되어야 한다고 생각된다. Background: Differentiated thyroid cancer is the most common endocrine malignancy. Despite advances in the treatment of thyroid cancer, disease recurrence and metastasis may occur in as many as 20% of patients, and so continues to pose major problems in its clinical management. Serum thyroglobulin (Tg) measurements, by immunoassay, are used to detect residual or recurrent thyroid cancer following thyriod ablation. However, the usefulness of immunoassay is limited by both the requirement for thyroid hormone withdrawal, to attain optimal test sensitivity, and interference by the antithyroglobulin antibody (Anti-Tg Ab). Recent studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of Tg mRNA in the peripheral blood of patients with differentiated thyroid carcinomas. We performed this study to evaluated the usefulness RT-PCR of Tg mRNA in peripheral blood of patients with thyroid carcinoma following a total thyroidectomy and radioiodine ablation therapy. Methods: Forty cases that underwent a total thyroidectomy and radioiodine ablation therapy were included in this study. Of the 40 patients, 35 were papillary carcinomas and 5 were follicular carcinomas. Ten normal control subjects were also studied. Tg mRNA was extracted. Then RT-PCR and nested RT-PCR, were run with specific Tg primers. Concurrently, DNA sequencing of the isolates was carried out to prove the isolates were identical to the nucleotide sequence of the Tg. Results: The Tg was detected in 4 of 19 patients, with either a residual thyroid bed, or metastasis, on a 131^I whole body scan and in 1 of 21 patients with a negative radioiodine scan. Surprisingly, the Tg mRNA was detected in all the patients and normal controls. Conclusion: From our results we can not recommend Tg mRNA, detected by RT-PCR in peripheral blood, as a tumor marker superior to that of the Tg serum level. We consider an intensive re-evaluation of the method is required before considering its clinical applications (J Kor Soc Endocrinol 17:501∼513, 2002).

Parts Feeder의 진동특성에 관한 연구(제2보) : Vibration Characteristics due to Bowl Materials Bowl의 재질에 관한 진동특성

김희남,권동호,김순채 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

In this paper, we have describes about the relationships between a core gap and a feeding velocity, an amplitude and the core gap by the exciting force, the parts movement and a bowl materials, and the feeding velocity and the weight of the parts in the parts feeder. The main results as compared with the stainless and the aluminum materials are as follow : 1) The mean feeding velocity by bowl material shown more relative proportion in the STS304 to the exciting voltage than the C5AV. 2) In the exciting voltage, the response time for the aluminum material is slow, but the stainless is sensibility. 3) An exciting voltage is rised by an increase of the weight, but an amplitude has been in the range between 23μm through 40μm.

Parts Feeder의 진동특성에 관한 연구

김순채,김희남,권동호 한국공작기계학회 1996 한국생산제조학회지 Vol.5 No.1

In this paper, we have studied about the relationships between a core gap and a feeding velocity, an amplitude and the core gap by the exciting force, the parts movement and a bowl materials, and the feeding velocity and the weight of the parts in the parts feeder. The obtained are as follow: 1) Optimal condition of mean feeding velocity is speeded up largely when the core gap is in 0.6mm. 2) It can be safe to say that the relation between the feeding velocity and the exciting voltage relay on the core gap. 3) An exciting voltage is rised by an increase of the weight, but an amplitude has been in the range between 23㎛ through 40㎛.

자연 동굴 토양 방선균의 속 다양성 분포

임채영,권오성,김판경,박동진,이동희,김창진 한국산업미생물학회 1996 한국미생물·생명공학회지 Vol.24 No.5

국내에 소재 하는 자연 동굴 중 충청북도의 고수굴, 천둥굴, 노동굴, 강원도의 고씨굴, 경상북도의 성류굴, 제주도의 협재굴, 쌍용굴, 만장굴 등 8개 동굴의 내부에 존재하는 토양 방선균 분포를 조사하였다. 그 결과 전체적인 속 수준의 분포로서는 Streptomyces가 52.5%, Micromonospora가 16.3%, Nocardioform 22.8% 정도 분포하고 있었으며 Kineosporia, Actinomadura, Nocardiopsis, Streptosporangium의 순서로 방선균이 0.3~1.4% 정도 분포하고 있었다. 지금까지 보고된 일반적인 방선균 분포와 비교해 볼 때 특히 Streptomyces의 분포 비율이 낮고 상대적으로 Micromonospora, Nocardioform이 많이 분포하고 있었다. 한편 노동굴 지역을 대상으로 동굴 내부와 외부로 나누어 방선균 분포를 비교해 보았다. 그 결과 동굴의 내부에는 Streptomyces가 52.0%, Micromonospora가 16.8%, Nocardia가 32.0% 정도 분포하였고 외부에서는 Streptomyces가 75.8%, Micromonospora가 10.7%, Nocardia가 7.1% 정도 분포하였다. 따라서 동일 지역 내에서도 동굴의 내부와 외부에 따라서 큰 차이를 나타내고 있었다. 그리고 분리된 방선균 중에서 Streptomyces속 균주의 형태적인 특징을 조사한 결과, 기균사의 색에 있어서는 회색 계열 45.7%, 적색 계열 7.8%, 황색 계열 2.3%, 녹색 계열 13.2%, 백색 계열 27.1%, 불량 3.9%의 분포를 나타내었다. 일반적으로 Streptomyces속 방선균에서 잘 분리되는 회색과 적색 계열의 분리 비율은 크게 낮아 졌으나 희소한 녹색과 백색 계열은 분리 비율이 크게 증가하였다. 그리고 성류굴에서는 황색, 녹색, 백색, 계열등 다양한 계열의 Streptomyces속 방선균이 많이 분리되었다. 포자 연쇄의 형태에 있어서는 retinaculiaperti 형태가 35.7%, spirales 형태가 12.4%, rectiflexibiles 형태가 51.9%로 나타났다. Total 276 soil actinomycete strains were isolated from 46 soil samples collected at domestic natural caves; the Kosu, Chundong, and Nodong caves at Chungbook province, the Kossi cave at Kangwon province, the Sungruye cave at Kyungbook province, the Hyupjae, Ssangyoug, and Manjang caves at Cheju province. All of these isolates were identified to the genus level based n morphological and physiological characteristics. As the result, 52.5% of those isolates were Streptomyces, 16.3% were Micromonospora, 22.8% were Nocardioform group, 1.1% were Actinomadura, 0.3% were Nocardiopsis, 0.3% were Streptosporangium, 0.3% were Nocardioides, 1.4% were Kineosporia, 4.7% wee the others. Streptomycete strains were the most abundant, but were relatively less comparing to general distribution pattern. Nocardioform and Micromonospora strains were quite abundant, and other rare actinomycete groups were somewhat abundant comparing to general distribution pattern previously reported. Especially Nocardioform strains were highly abundant at almost of the natural caves.

제조업 근로자들의 혈청 Perfluorooctanoic Acid (PFOA) 농도와 생활습관 및 직업적 관련요인

장상환,이채관,김대환,김권복,안진홍,김휘동,이창희,김정호,이종태 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.3

목적: PFOA에 노출이 가능한 제조업 근로자들을 대상으로 혈청 PFOA농도를 조사하고 생활습관, 직업적 요인, 간기능 및 혈청 지질과의 관련성을 연구하고자 하였다. 방법: 2007년 8월부터 12월까지 부산지역 1개 대학병원 산업의학과에서 건강진단을 받은 PFOA노출 가능성이 있는 제조업 사업장 근로자 남자 203명과 여자112명의 총315명을 대상으로 하였다. 생활습관 및 직업적 요인을 설문조사 하였고, 혈청 PFOA 농도를 LC-MS/MS로 분석하였으며, 간기능 및 혈청 지질 검사를 실시하였다. 결과: 혈청 PFOA 농도의 평균은 전체가 8.0 ng/mL이었으며, 남자가 9.0 ng/mL, 여자가 6.3 ng/mL로 남자가 여자보다 통계적으로 유의하게 높았다(p<0.001). 다중회귀분석을 시행한 결과 성, 연령, 체질량지수, 총 콜레스테롤 등이 유의하게 나타났으며, 성별로는 남성에서, 연령이 많을 수록, 총 콜레스테롤이 높을 수록, 체질량지수가 낮을 수록 혈청 PFOA의 농도가 통계적으로 유의하게 높았다. 로지스틱 회귀분석을 실시하여 성과 연령을 보정한 교차비를 산출한 결과, 섬유제품 염색, 정리 및 마무리 가공업(OR 6.16)이 통계적으로 유의하게 높았고, 주형 및 금형 제조업(OR 4.84), 선박 구성부분품 제조업 (OR 3.87), 플라스틱 합성피혁 제조업 (OR 10.05) 등이 상대적으로 높았다. 결론: 제조업 근로자들의 혈청 PFOA 농도는 성, 연령 및 총 콜레스테를 등과 관련성이 있었고, 업종별로는 섬유제품 염색, 정리 및 마무리 가공업에서 높은 것으로 조사되었다. 이러한 결과는 PFOA의 인체 위해성을 조사하기 위한 기초자료로 활용가치가 있을 것이다. Objectives: The objective of this study was to determine serum perfluorooctanoic acid (PFOA) concentrations and to investigate the relationship of lifestyle, health status and work-related factors among workers at manufacturing companies with possible occupational exposure to PFOA. Methods: The study subjects were 315 workers (male 203, female 112) of manufacturing companies with possible exposure to PFOA from August to December, 2007. A questionnaire was administered to investigate lifestyle and work-related factors. Serum PFOA concentrations were analyzed by LC-MS/MS, and liver function and total cholesterol were also tested. Results: Serum PFOA concentrations ranged from 1.0 to 40.9 ng/mL (mean 8.0 ng/mL). Serum PFOA concentrations increased with age. Male workers had higher PFOA concentration than female workers (male 9.0 ng/mL; female 6.3 ng/mL). According to stepwise multiple regression analysis, factors significantly associated with PFOA concentration were male, being older, higher total cholesterol, and lower body mass index. Adjusted for age and gender by logistic regression, dyeing and finishing textiles had significantly higher odds ratio (OR) of 6.16, and moulding patterns, moulds and industrial patterns (OR=4.84), sections for ships (OR=3.87), and plastics synthetic leather (OR=1O.05) had marginally significantly high odds ratios. Conclusions: This study demonstrated the factors affecting serum PFOA concentration of workers at manufacturing companies. Further study is needed to ensure the effect of occupational exposure on serum PFOA concentration.

Schisandrin A suppresses lipopolysaccharide-induced inflammation and oxidative stress in RAW 264.7 macrophages by suppressing the NF-κB, MAPKs and PI3K/Akt pathways and activating Nrf2/HO-1 signaling

Kwon, Da Hye,Cha, Hee-Jae,Choi, Eun Ok,Leem, Sun-Hee,Kim, Gi-Young,Moon, Sung-Kwon,Chang, Young-Chae,Yun, Seok-Joong,Hwang, Hye Jin,Kim, Byung Woo,Kim, Wun-Jae,Choi, Yung Hyun UNKNOWN 2018 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.41 No.1

<P>Schisandrin A is a bioactive lignan occurring in the fruits of plants of the <I>Schisandra</I> genus that have traditionally been used in Korea for treating various inflammatory diseases. Although the anti-inflammatory and antioxidant effects of lignan analogues similar to schisandrin A have been reported, the underlying molecular mechanisms have remained elusive. In the present study, schisandrin A significantly suppressed the lipopolysaccharide (LPS)-induced production of the key pro-inflammatory mediators nitric oxide (NO) and prostaglandin E<SUB>2</SUB> by suppressing the expression of inducible NO synthase and cyclooxygenase-2 at the mRNA and protein levels in RAW 264.7 macrophages. Furthermore, schisandrin A was demonstrated to reduce the LPS-induced secretion of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β; this was accompanied by a simultaneous decrease in the respective mRNA and protein levels in the macrophages. In addition, the LPS- induced translocation of nuclear factor-κB (NF-κB), as well as activation of mitogen-activated protein kinases (MAPKs) and phosphatidylinositol-3 kinase (PI3K)/Akt pathways were inhibited by schisandrin A. Furthermore, schisandrin A significantly diminished the LPS-stimulated accumulation of intracellular reactive oxygen species, and effectively enhanced the expression of NF erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). These results suggested that schisandrin A has a protective effect against LPS-induced inflammatory and oxidative responses in RAW 264.7 cells by inhibiting the NF-κB, MAPK and PI3K/Akt pathways; these effects are mediated, at least in part, by the activation of the Nrf2/HO-1 pathway. Based on these results, it is concluded that schisandrin A may have therapeutic potential for treating inflammatory and oxidative disorders caused by over-activation of macrophages.</P>

Gomisin N has anti-allergic effect and inhibits inflammatory cytokine expression in mouse bone marrow-derived mast cells

Chae, Hee-Sung,Kang, Ok-Hwa,Oh, You-Chang,Choi, Jang-Gi,Keum, Joon-Ho,Kim, Sung-Bae,Kim, Yong-Sik,Mun, Su-Hyun,Shin, Dong-Won,Han, Sin-Hee,Kwon, Dong-Yeul Informa Healthcare 2011 Immunopharmacology and immunotoxicology Vol.33 No.4

<P>Gomisin N is a bioactive compound and a prominent anti-allergic agent found in the fruits of tree <I>Schizandra chinensis</I>. However, its effects on the bone marrow-derived mast cell (BMMC)-mediated allergy and inflammation mechanism remain unknown. In this study, the biological effects of gomisin were evaluated while focusing on its effects on the allergic mediator in PMA ++ A23187-stimulated BMMCs. The anti-allergic effect of gomisin has shown that inhibited PMA ++ A23187-induced interleukin-6 (IL-6) production. An investigation was also conducted to determine its effects on the production of several allergic mediators including prostaglandin D<SUB>2</SUB> (PGD<SUB>2</SUB>), leukotriene C<SUB>4</SUB> (LTC<SUB>4</SUB>), β-hexosaminidase (β-Hex), and cyclooxygenase-2 (COX-2) protein. The results revealed that gomisin inhibited the PMA ++ A23187-induced production of IL-6, PGD<SUB>2</SUB>, LTC<SUB>4</SUB>, β-Hex, and COX-2 protein. Taken together, these findings indicate that gomisin N has the potential for use in the treatment of allergy.</P>

HCV, Acute, LT : Epidemiologic Study for the Risk Factors of HCV Transmission

( Hee Bok Chae ),( Byung Seok Lee ),( An Na Kim ),( Tae Hee Lee ),( Soon Young Ko ),( Hong Soo Kim ),( Il Han Song ),( Jung Il Lee ),( Jeong Heo ),( Ki Tae Yoon ),( Oh Sang Kwon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: In Korea, 1-2% of population is infected, and 15-20% of them have chronic liver diseases from HCV infection. On an annual basis, 3-4 million people are infected through blood transfusion, reckless drug abuse sexual contact, and exposure during childbirth. We want to know what risk factors contribute significantly to HCV infection in Korea. Methods: We surveyed 149 HCV-infected patients and 146 healthy controls (overall group) with a questionnaire to find out the risk factors related to HCV transmission. Results: The results were as follows. The statistically significant factors were ex-drinker, current smoking, history of acute hepatitis, having a HCV-positive family member, history of blood transfusion, acupuncture, moxa cautery, tatooing, sharing a razor, history of IV drug use, history of inhaled hallucinogen, more than one sexual partner, and history of venereal disease. We also performed a subgroup analysis according to their place of residence, inland group and coastal region group. The coastal region group was composed of 40 HCV-infected patients and 40 healthy controls. The statistically significant factors were ex-drinker, history of moxa cautery, tatooing, history of IV drug use, and history of inhaled hallucinogen. The inland group was composed of 106 HCV infected patients and 106 healthy controls. The statistically significant factors of inland group were similar to those of overall group. We found significant factors with multivariate analysis were revealed as follows, IV drug use (OR 14.73), history of acute hepatitis (OR 12.55), history of alcohol drinking (OR 7.46), tatooing (OR 3.64), moxa cautery (OR 3.64). Conclusions: The most important risk factor was IV drug use. The proportion of IV drug use patients in coastal region was two times as that of inland.(25% vs. 11.3%) Eighty seven percent of IV drug use patients experienced sharing of syringe with other people. Needle exchange program is recommended. Also, anti-HCV antibody test should be included in the test list of national health screening program, for people at age 40.

PRODUCTION AND CHARACTERIZATION OF POLYCLONAL ANTIBODIES TO THE MULTIFUL ANTIGENIC PEPTIDES ( MAP )

Kwon Suk Chae,Myung Hee Nam,Joo Hee Chung,Jong Bok Seo,Oh Oak Kwon 생화학분자생물학회 1997 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-