Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)

양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.

가족공동체를 떠난 간호대학생들의 대학생활체험

김고은,김유은,김희진,조유진,안주영,허다연,홍서우,차지영,박송이 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: The purpose of this study was to understand and explain the experiences of college life among junior nursing students who live apart from their families. Methods: 14 junior nursing students living apart from their families for at least one semester were recruited through snowballing and convenience sampling. Participants were interviewed individually using open ended questions. Data were collected from July to October in 2017 until data saturation. Interviews were transcribed verbatim and analyzed by using hermeneutic phenomenological method proposed by van Manen. Results: Five essential theme clusters emerged: (1) faced with diverse problems while living apart from family, (2) depend on family when stressed, (3) adapt to college life by being independent and forming self-initiative lifestyle, (4) confront with difficulties once again when adapting to academic curriculum restructured by practicum, (5) pave the way to nursing career and feel proud of overcoming the difficulties independently. Conclusion: Although most participants faced diverse problems in college life, they tried to adapt independently by forming self-initiative lifestyle and overturn the difficulties to positive experiences. In conclusion, the process of living apart from families transformed participants into proactive individuals. Adopting counseling programs could support starting their junior years successfully.

Development of bulk heterojunction morphology by the difference of intermolecular interaction behaviors

Cha, H.,Baek, J.Y.,An, T.K.,Kim, S.O.,Kwon, S.K.,Kim, Y.H.,Park, C.E. Elsevier Science 2014 ORGANIC ELECTRONICS Vol.15 No.12

The morphology of a bulk heterojunction can be controlled by adding a processing additive in order to improve its power conversion efficiency (PCE) in photovoltaic devices. The phase-separated morphologies of blends of PONTBT or P3HT with fullerene derivatives are systematically examined in the presence of processing additives that possess various alkane alkyl chain lengths or end-group electronegativities. We determined the morphologies of the bulk heterojunction layers by using atomic force microscopy (AFM) and grazing incidence wide angle X-ray scattering (GIWAXS). The photocurrent-voltage characteristics of the bulk heterojunction solar cells were found to be strongly dependent on the intermolecular interactions between the conjugated polymers, the fullerene derivatives, and the processing additives in the photoactive layer. The optimal PONTBT:fullerene derivative blend morphology was obtained with a processing additive, 1,3-diiodopropane (1,3-DIP), that possesses a short alkyl chain and an end group with weak electronegativity, and was found to exhibit a high fill factor (FF) and a high current density (J<SUB>SC</SUB>). In contrast, in blends of P3HT with the fullerene derivative, PCEs with higher FF and J<SUB>SC</SUB> values were achieved by incorporating the processing additive, 1,8-dibromooctane (1,8-DBrO), which has a long alkyl chain and a strong electronegative end group. Thus the selection of the processing additive with the aim of enhancing photovoltaic performance needs to take into account the intermolecular interaction of the conjugated polymer.

Development of Continuous Manufacturing Process for High-performance Thin Film Ni-YSZ Anode and Anode Functional Layer by Co-sputtering Method for Solid Oxide Fuel Cells

Y. J. Kim(김양재),S. Lee(이상훈),S. W. Cha(차석원) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.11월

The process of manufacturing an anode functional layer (AFL) in the conventional SOFC is inefficient, because, typically, the powder is ball-milled for a long time and sintered above 1,200°C. Also, in thin-film SOFCs, after fabricating the anode, a new target material or precursor is loaded on the vacuum deposition equipment to deposit the AFL. Alternatively, transferring the cell to other thin-film deposition equipment to lay the AFL requires more time and labor. Therefore, this study developed a process to fabricate an anode and AFL through the co-sputtering process continuously. By applying DC power to Ni and RF power to the Y/Zr metal alloy target, a Ni-YSZ anode of 700 nm and an AFL of 150 nm were continuously fabricated. This one-step fabrication process of an anode and AFL controls the proportion of the deposited material by adjusting DC and RF’s sputtering power. The film deposited with various power ratios was applied to anodic aluminum oxide wafers to characterize its electrochemical performance as SOFCs. XPS and EDS analyses were performed to confirm the compositional change according to the application of power. FESEM, AFM, and FIB-SEM analyses were performed to identify the microstructure change according to YSZ content.

운송하역 향상을 위한 Y/T 정차유도 프로토타입 장치 연구

김동훈(D. H. Kim),송준엽(J. Y. Song),차석근(S. K. Cha),강일용(I. Y. Kang),이승호(S. H. Lee) 한국정밀공학회 2009 한국정밀공학회 학술발표대회 논문집 Vol.2009 No.10월

Y염색체 장완 결실을 동반한 무정자증 1례

남윤성,김현주,이숙환,곽인평,윤태기,차광열,Nam, Y.S.,Kim, H.J.,Lee, S.H.,Kwak, I.P.,Yoon, T.K.,Cha, K.Y. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

Different Y mutation in Yq11 occurring de novo in sterile males were first described 19 years ago. Since the phenotype of the patients was always associated with azoospermia or severe oligospermia, it was postulated that these mutations interrupt a Y spermatogenesis locus in the euchromatic Y region (Yq11) called azoospermia factor (AZF). Recently, it became possible to map AZF mutations to different subregions in Yq11by molecular deletion mapping. This indicated that azoospermia is possibly caused by more than one Y gene in Yq11 and the Yq11 chromatin structure. The frequency of AZF mutations in idiopathic sterile males $(5{\sim}20%)$ may indicate a need for a general screening programme for its analysis in infertility clinic. We have experienced a case of deletion distal to Yq11 region in azoospermic patient. So we report this case with a brief review of literatures.

Cyclobuxine D의 토끼 적출 장관에 대한 작용

이종화,박영현,조병헌,김종배,김정목,김유재,김천숙,차영덕,김영석 최신의학사 1988 最新醫學 Vol.31 No.1

JBR(Jeju Island Bovine Rotavirus)의 Sequencing에 의한 Homology 비교에 관한 연구

이종익,谷口孝喜,김응률,류영수,박선오,송진욱,유제현,조홍찬,차광종,浦澤正三,이태협,김유성,이영건 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

본 연구는 PCR에 의해 로타바이러스의 serotype을 결정하고 염기배열의 유사성을 다른 지역에서 발견된 로타바이러스와 비교 분석하였으며 그 결과는 다음과 같다. 1. 제주도 목장에서 송아지 설사변을 채취하여 로타바이러스를 분리 후 MA104세포에 감염시켜 세포변성 효과(CPE)를 확인하였다. 2. 전자현미경에 의해 형태학적로 로타바이러스임이 판명되었으며 형광항체법에 의해 MA104세포에 감염된 것을 재확인 하였다. 3. 제주도 송아지 로타바이러스(JBR)RNA를 PAGE에 의해 genotype를 분석한 결과 bovine특유의 4:2:3:2 pattern이지만 일반 PAGE로 분석한 결과는 NCDV, UK, KK3, A5-37, 61A, B223와 차이가 있었으며, Ns-5, Nc-5, Kawatabi(Japan)와는 비슷한 경향을 보였다. 4. Plaquing 후 titer한 결과 NCDV, UK보다는 낮은 ??PFU/ml이었다. 5. RNA-RAN hybridization과 ELISA 및 VP7과 VP4의 1차, 2차 PCR 사물을 1% agarose(EtBr 1㎕의 TAE)에서 전기영동한 결과 G6P11의 serotype이었다. 6. JBR의 P serotype이 동일하게 나타난 B223과의 비교 결과 총 JVP8 750 bases 중에서 731개의 염기가 B223과 같은 것으로 나타나 염기배열의 유사성은 97.47%로 나타났고, 아미노산 배열의 유사성은 97.57%로 나타났다. This study was carried our to identify JBR's serotype by polymerase chain reaction(PCR) and to analyze homology of JBR' sequence. The results obtained in these experiments were as follows: 1. Fecal samples of calf diarrhea were taken on farms in Jeju island, rotavirus was isolated and cytopathic effect(CPE) was determined after infection to MA104 cell. 2. Morphological evaluation on electron microscopy proved it as rotavirus. Also, its infection to MA104 cell was reidentified using a fluorescence antibody method. 3. Genotype of Jeju island bovine rotavirus(JBR) analyzed thorough PAGE was 4:2:3:2 pattern, which was unique in bovine and that analyzed through general PAGE was somewhat different from NCDV, UK, KK3, A5-37, 61A, B223 and similar to Nstool-5, N cultrue-5 and Kawatabi(Japan). 4. By titration after plaquing, the level was ??PEU/ml, which was lower than those of NCDV and UK. 5. Electrophoresis analysis of RNA-RNA hybridization. ELISA, adn first and second PCR products of VP7 and VP4 in 1% agarose(TAE+1㎕ EtBr) revealed that the rotavirus was a serotype of G6P11. 6. Alignment of two(JBR and B223) amino acid sequences is 97.57% and nucleotide sequences is 97.47%.

흰쥐 적출 심근의 수축 현상에 대한 cyclobuxine과 칼슘 효현제인 Bay K 8644의 상호작용

박영현,김유재,김종배,차영덕,이종화,조병헌,권준택,김창호 순천향대학교 1992 논문집 Vol.15 No.2

Cyclobuxine, the major alkaloid of Buxus microphylla var. koreana Nakai, is a steroidal alkaloid recognized to contain a cyclopropane ring and has a substitution pattern at C-4 and C-14. In our previous studies, cyclobuxine blocked potassium-activated calcium channels(voltage-sensitive calcium channels) in ileal smooth muscle. Cyclobuxine also exerted negative chronotropic and inotropic effects in the anesthetized rat and the isolated rat heart. In this study, antagonism between either the dihydropyridine calcium agonist, Bay K 8644, and high external Ca++ or nifedipine, verapamil, diltiazem, and cyclobuxine was investigated in the isolated ventricular muscle from rats. Bay K 8644(?? M) produced a slowly developing increase in developed tension. Cyclobuxine and the calcium antagonists decreased dose-dependently developed tension. Bay K 8644(??M) produced a rightward parallel shift of the concentration-response curves for the negative inotropic effects of nifedipine(??). Bay K 8644 produced only a slight rightward shift of the concentration-response curves for the negative inotropic effects of diltiazem(??) and cyclobuxine (??). These results indicate that Bay K 8644 antagonizes the negative inotropic effect of nifedipine by competing with the same site closely associated with the calcium channel. In contrast, Bay K 8644 antagonizes noncompetitively the negative inotropic effects of cyclobuxine, verapamil and diltiazem in the isolated ventricular muscle.

MSV로 변이시킨 가토신장세포의 배양조건에 따른 지방조성변화에 대한 연구

최한용,박주홍,김화영,김영래,이인수,조영준,박재윤,차종희,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Differencies of lipid composition of murine sarcoma virus 97017 transformed rabbit kidney cells and their plasma membranes in monolayer and suspension culture were studied. Free cholesterol was the major neutral lipid extracted from the whole cells and plasma membranes of all these cultures. Triglyceride increased about 6 to 50 folds in suspension grown whole cells and their plasma membranes compared to those from monolayer cultures. The μ mole of total neutral lipids and phospholipids are elevated in plasma membranes compared to whole cells from monolayer cultures bur arc similar in suspension cultures. The distribution of fatty acids in the major lipid classes of plasma membranes and whole cells generally follow the composition of calf serumused in the culture media.