유방의 세침흡인 세포검사: 수기 액상세포검사법(Liqui-$PREP^{TM}$)과 고식적 도말법과의 비교

정지윤,김정식,김영수,김혜정,박지영,Jeong, Ji-Yun,Kim, Jeong-Shik,Kim, Young-Su,Kim, Hye-Jung,Park, Ji-Young 대한세포병리학회 2008 대한세포병리학회지 Vol.19 No.1

Fine needle aspiration (FNA) cytology of the breast is a useful method for diagnosing breast lesions. Yet making the definite diagnosis with performing FNA is limited by some problems, such as the low cellularity, the poor preservation and the obscuring background. Recent studies have found that liquid-based cytology solves such problems, but it is an expensive method and it is limited by the loss of the background information. The purpose of this study is to compare the Liqui-$PREP^{TM}$, a new manual liquid-based method of cytology, and the conventional smears for analyzing breast FNA cytology materials. A total of 31 randomized FNA specimens of breast were studied. In each case, both the conventional smears and the Liqui-$PREP^{TM}$ method were performed, and the smears were evaluated for cellularity, cellular preservation, the background, the cytologic features and the architectural arrangement. The cellularity and architectural arrangement were equal for both preparations. The Liqui-$PREP^{TM}$ specimens showed better cellular preservation, loss of the obscuring background, no overlapping of cells and a smaller area to screen compared with the conventional smears. Moreover, it has the potential advantages of being able to use the remaining specimens for immunohistochemical study and ploidy analysis, and it can reduce the costs for preparation compared with the other liquid-based methods of cytology. But some background information is lost in the Liqui-$PREP^{TM}$ specimens, the same as the other liquid-based methods of cytology. In conclusion, the Liqui-$PREP^{TM}$ and conventional smears showed good correlation, but they have their respective advantages and disadvantages. These results suggest that Liqui-$PREP^{TM}$ can contribute to making the accurate diagnosis with performing breast FNA cytology when it is used along with other methods.

음양곽(淫羊藿) 열수 추출물의 Smad 신호 억제를 통한 간성상세포의 활성 조절

정지윤,민병구,박정아,변성희,조일제,김상찬,Jung, Ji Yun,Min, Byung-Gu,Park, Chung A,Byun, Sung Hui,Cho, Il Je,Kim, Sang Chan 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.3

Objectives : In Traditional Korean Medicine, Epimedium koreanum Nakai has diverse pharmacological activities to treat impotence, forgetfulness, cataract and exophthalmos. Present study investigated anti-fibrogenic effects of E. koreanum water extract (EKE) in hepatic stellate cells (HSCs). Methods : To study anti-fibrogenic effects of EKE, LX-2 cells, a human immortalized HSCs, were pre-treated with $3-300{\mu}g/mL$ of EKE, and then subsequently exposed to 5 ng/mL of transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$). Expression level of ${\alpha}-smooth$ muscle actin was determined by immunoblot analysis. Phosphorylation of Smad, transactivation of Smad, and expression of plasminogen activator inhibitor-1 (PAI-1) were monitored to investigate the effect of EKE on $TGF-{\beta}1-mediated$ signaling pathway. Results : Up to $100{\mu}g/mL$, EKE did not show any cytotoxicity on LX-2 cells. Pre-treatment of EKE ($100{\mu}g/mL$) significantly inhibited ${\alpha}-smooth$ muscle actin expression induced by $TGF-{\beta}1$. In addition, EKE significantly decreased Smad2 and Smad3 phosphorylations, Smad binding element-driven luciferase activity and PAI-1 expression by $TGF-{\beta}1$. Of three flavonoid compounds found in EKE, only quercertin ($30{\mu}M$) attenuated $TGF-{\beta}1-mediated$ PAI-1 expression. Conclusion : These results suggest that EKE has an ability to suppress fibrogenic process in HSCs via inhibition of $TGF-{\beta}1/Smad$ signaling pathway.

면역기능 증강 신물질에 대한 마우스의 면역학적 및 혈액학적 변화

정지윤,Jung, Ji-Youn 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

Immu-Forte composed of chitosan, ${\beta}-glucan$, manno-oligosaccharide and pangamic acid was evaluated for its effectiveness as a nonspecific immunostimulator in mice. The effects of Immu-Forte were determined by analysis of cytokines using ELISA and phenotype of leukocyte subpopulations using monoclonal antibodies specific to mouse leukocyte differentiation antigens and flow cytometry. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in Immu-Forte A-treated group increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages and IL-2 in Immu-Forte EX-treated low and middle dose groups increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In the Immu-Forte soybean-treated group, NK cells and IL-4 were significantly higher in middle dose-treated group, and IL-2, IL-4 and IFN-r were significantly higher in low dose-treated group. In the Immu-Forte F-treated group, all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, NK cells, IL-2, IL-4, IL-12 and IFN-r in high dose-treated group and all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in middle dose-treated group and NK cells, IL-2, IL-4, IL-12 and IFN-r in low dose-treated group were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In conclusion, this study has demonstrated that Immu-Forte had an immunostimulatory effect on mice through proliferation and activation of mouse immune cells.

실험동물에서의 환경호르몬 물질의 생체내 영향 및 검색법 정립에 대한 연구

정지윤,이영순,Jung, Ji-Youn,Lee, Yong-Soon 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. Chemicals were DEHP, DBP, BPA and NP, were injected sc once daily with combinations of chemicals treatments for 3 days. In the results, the reported estrogenic chemicals DEHP and DBP were both negative in the single dose treatments. But, in the combinations of chemicals treatments, DEHP and DBP increased in bud number of mammary gland. Treatment of ovariectomized mice with combinations of other chemicals resulted in uterine and vaginal hyperplasia. The additive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and DBP treatment. the competitive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and nonylphenol, $17{\beta}$-Bestradiol and bisphenol-A treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. it provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals. The results described illustrate the intrinsic complexity of evaluating chemicals for estrogenic activities and conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.

Intralipidos에 대한 변이원성시험

정지윤,이원우,임종희,남정석,제정환,이광훈,강병철,이병희,박재학,이영순,Jung, Ji-Youn,Lee, Won-Woo,Ihm, Jong-Hee,Nam, Jeong-Seok,Che, Jeong-Hwan,Li, Guang-Xun,Kang, Byeong-Cheol,Yi, Beoung-Hi,Park, Jae-Hak,Lee, Yong-Soon 한국독성학회 1998 Toxicological Research Vol.14 No.3

In order to evaluate the mutagenic potential of Intralipidos produced by Greenmate cooperation. We performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells. In the reverse mutation test using Salmonella typhimurium TA98 and TA100, Intralipidos did not increase the number of revertant at any of the concentration tested in this study. Intralipidos did not increase the number of cells having structural or numberical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase were observed in the occurrence of micornucleated polychromatic erythrocytes in ICR male mice intraperitoneally administered with Intralipidos. These results indicate that Intralipidos has no genetic toxicity under these experimental conditions.

교통사고로 인한 중증손상 환자에 대한 분석

정지윤 ( Ji Yoon Jung ),백경원 ( Kyung Won Paek ),이국종 ( Kug Jong Lee ),조준필 ( Joon Pil Cho ) 대한외상학회 2004 大韓外傷學會誌 Vol.17 No.2

Background: To provide information about serious traffic accident injuries in Suwon, Korea, during the year of 2000, we conducted an epidemiologic study of injured patients. Methods: The data were obtained by reviewing medical records of patients requiring hospital admission due to traffic injury. A retrospective study, the selection of patients was made according to ICD-10 code. Results: A total of 1389 patients were surveyed. With respect to the causes of injury, traffic accidents accounted for the highest percentage (40.2%). More men than women were injured in all age groups. The highest frequency of traffic injuries was noted in patients ranging in age from 20 to 39 years. The occurrences of accidents were distributed evenly throughout the year, with little seasonal correlation. As for the time of day, the accidents occurred more often in the afternoon and evening. Most of patients were admitted to general ward. The mean ISS of all traffic accident patients was 9.41±7.81. Factors associated with injury severity were sex, age, road user, time of day, and results of ED treatment. Conclusion: This is the first study of serious traffic accident injuries in Suwon for the year 2000. As such, our findings constitute the seminal information for future research, including collection of further data, analyses, and extensions to other cities.

문화ㆍ예술의 도시 이미지 개선을 위한 시각적 아이덴티티의 적용에 관한 고찰

정지윤(Jung Jee-Youn),김남훈(Kim Nam-Hoon) 한국디자인학회 2005 한국디자인학회 학술발표대회 논문집 Vol.2005 No.10

EU 난민정책 형성과정에서 집행위원회(European Commission)의 역할

정지윤(Ji Yun Jung),박성호(Sung Ho Park) 연세대학교 동서문제연구원 2017 동서연구 Vol.29 No.3

본 연구는 다층거버넌스 (multi-level governance, MLG)이론에 기반하여, 유럽연합(European Union, EU)의 난민정책 형성과정을 분석한다. 특히 EU 집행위원회(European Commission)가 정책결정에 미친 영향력에 초점을 두고, 이를 설명하는 초국적 인과메커니즘을 탐구한다. 핵심가설은 집행위원회가 EU회원국 및 이사회 기구에 정책결정의 영향력을 행사하는 데에, 해당 이슈영역의 권위적 국제기구인 UN난민기구(United Nations High Commissioner for Refugees, UNHCR)와의 초국적 정책연합이 중요 요인으로 작용했다는 것이다. 이러한 가설을 검증하기 위해 본 연구는 현 EU 난민정책의 근간이 형성된 2000년부터 2005년까지의 주요 정책의 도입과정을 살펴본다. This article applies multi-level governance theory to examine refugee policy making in the European Union (EU). The authors focus on the role of European Commission in the policy making process, identifying a transnational causal mechanism which explains the Commission s varying influence vis-a-vis member states and other EU institutions. The core argument is that the Commission s influence depended on whether or not it could build a transnational policy coalition with UNHCR(United Nations High Commissioner for Refugees), the most established international organization on refugee issues. The authors support their claim by examining major refugee policies, introduced during the 2000-2005 periods, which established the foundation of the EU refugee system.

눈꺼풀로 전이된 진행성 위암

정지윤 ( Ji Yoon Jung ),구은주 ( Eun Joo Goo ),이재창 ( Jae Chang Lee ),송재이 ( Jay Song ),고성애 ( Sung Ae Koh ),이경희 ( Kyung Hee Lee ),배영경 ( Young Kyung Bae ) 영남대학교 의과대학 2016 Yeungnam University Journal of Medicine Vol.33 No.2

Gastric cancer is the most common cancer in Korean males and can easily spread to distant organs such as the liver, lungs, brain, or bones. However, skin metastasis, particularly of the eye, is rare. Metastatic eyelid cancer is extremely rare; metastases from internal organs have not been reported so far. We recently experienced a patient with metastatic eyelid cancer from adenocarcinoma of the stomach. A 62-year-old female was admitted with a right upper eyelid mass and foreign body sensation. She had a history of stomach cancer of 3 years. She was treated by chemotherapy and radiotherapy for pathologic fracture. After receiving supportive care for 2 years, the mass appeared on her right pupil. Punch-biopsy of the mass was performed and histological examination revealed adenocarcinoma, the same as the initial histological result. We report this case with a review of related literature.

타목시펜과 프레드니솔론으로 호전된 경화성 피막성 복막염 (sclerosing encapsulating peritonitis)

정지윤 ( Ji Yoon Jung ),장원익 ( Won Ik Jang ),윤지현 ( Ji Hyun Yoon ),김의식 ( Eui Sik Kim ),정사라 ( Sa Rah Chung ),최대은 ( Dae Eun Choi ),나기량 ( Ki Ryang Na ),이강욱 ( Kang Wook Lee ),강대영 ( Dae Young Kang ),신영태 ( Young 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.6

Sclerosing encapsulating peritonitis (SEP) is an uncommon but serious complication of long-term peritoneal dialysis (PD). Entrapment of the intestine in fibrous tissue, causing complete intestinal obstruction, is referred to as SEP. The usual clinical presentation is with partial or complete small bowel obstruction, ascites, abdominal mass, or impaired peritoneal ultrafiltration. Conservative treatment carries a poor outcome and surgery has offered variable results. Even though there is no established medical treatment, immunosuppressive drugs, steroid and tamoxifen are often used. Tamoxifen is a nonsteroidal anti-estrogenic drug commonly used in the management of breast cancer. To our knowledge, this is the first case of sclerosing encapsulating peritonitis successfully treated with tamoxifen and prednisolone in Korea. Recently, we have treated three SEP patients with tamoxifen and prednisolone. All three patients showed clinical improvement within a few months.