면역기능 증강 신물질에 대한 마우스의 면역학적 및 혈액학적 변화

정지윤,Jung, Ji-Youn 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

Immu-Forte composed of chitosan, ${\beta}-glucan$, manno-oligosaccharide and pangamic acid was evaluated for its effectiveness as a nonspecific immunostimulator in mice. The effects of Immu-Forte were determined by analysis of cytokines using ELISA and phenotype of leukocyte subpopulations using monoclonal antibodies specific to mouse leukocyte differentiation antigens and flow cytometry. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in Immu-Forte A-treated group increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages and IL-2 in Immu-Forte EX-treated low and middle dose groups increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In the Immu-Forte soybean-treated group, NK cells and IL-4 were significantly higher in middle dose-treated group, and IL-2, IL-4 and IFN-r were significantly higher in low dose-treated group. In the Immu-Forte F-treated group, all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, NK cells, IL-2, IL-4, IL-12 and IFN-r in high dose-treated group and all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in middle dose-treated group and NK cells, IL-2, IL-4, IL-12 and IFN-r in low dose-treated group were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In conclusion, this study has demonstrated that Immu-Forte had an immunostimulatory effect on mice through proliferation and activation of mouse immune cells.

Intralipidos에 대한 변이원성시험

정지윤,이원우,임종희,남정석,제정환,이광훈,강병철,이병희,박재학,이영순,Jung, Ji-Youn,Lee, Won-Woo,Ihm, Jong-Hee,Nam, Jeong-Seok,Che, Jeong-Hwan,Li, Guang-Xun,Kang, Byeong-Cheol,Yi, Beoung-Hi,Park, Jae-Hak,Lee, Yong-Soon 한국독성학회 1998 Toxicological Research Vol.14 No.3

In order to evaluate the mutagenic potential of Intralipidos produced by Greenmate cooperation. We performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells. In the reverse mutation test using Salmonella typhimurium TA98 and TA100, Intralipidos did not increase the number of revertant at any of the concentration tested in this study. Intralipidos did not increase the number of cells having structural or numberical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase were observed in the occurrence of micornucleated polychromatic erythrocytes in ICR male mice intraperitoneally administered with Intralipidos. These results indicate that Intralipidos has no genetic toxicity under these experimental conditions.

실험동물에서의 환경호르몬 물질의 생체내 영향 및 검색법 정립에 대한 연구

정지윤,이영순,Jung, Ji-Youn,Lee, Yong-Soon 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. Chemicals were DEHP, DBP, BPA and NP, were injected sc once daily with combinations of chemicals treatments for 3 days. In the results, the reported estrogenic chemicals DEHP and DBP were both negative in the single dose treatments. But, in the combinations of chemicals treatments, DEHP and DBP increased in bud number of mammary gland. Treatment of ovariectomized mice with combinations of other chemicals resulted in uterine and vaginal hyperplasia. The additive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and DBP treatment. the competitive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and nonylphenol, $17{\beta}$-Bestradiol and bisphenol-A treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. it provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals. The results described illustrate the intrinsic complexity of evaluating chemicals for estrogenic activities and conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.

유방의 세침흡인 세포검사: 수기 액상세포검사법(Liqui-$PREP^{TM}$)과 고식적 도말법과의 비교

정지윤,김정식,김영수,김혜정,박지영,Jeong, Ji-Yun,Kim, Jeong-Shik,Kim, Young-Su,Kim, Hye-Jung,Park, Ji-Young 대한세포병리학회 2008 대한세포병리학회지 Vol.19 No.1

Fine needle aspiration (FNA) cytology of the breast is a useful method for diagnosing breast lesions. Yet making the definite diagnosis with performing FNA is limited by some problems, such as the low cellularity, the poor preservation and the obscuring background. Recent studies have found that liquid-based cytology solves such problems, but it is an expensive method and it is limited by the loss of the background information. The purpose of this study is to compare the Liqui-$PREP^{TM}$, a new manual liquid-based method of cytology, and the conventional smears for analyzing breast FNA cytology materials. A total of 31 randomized FNA specimens of breast were studied. In each case, both the conventional smears and the Liqui-$PREP^{TM}$ method were performed, and the smears were evaluated for cellularity, cellular preservation, the background, the cytologic features and the architectural arrangement. The cellularity and architectural arrangement were equal for both preparations. The Liqui-$PREP^{TM}$ specimens showed better cellular preservation, loss of the obscuring background, no overlapping of cells and a smaller area to screen compared with the conventional smears. Moreover, it has the potential advantages of being able to use the remaining specimens for immunohistochemical study and ploidy analysis, and it can reduce the costs for preparation compared with the other liquid-based methods of cytology. But some background information is lost in the Liqui-$PREP^{TM}$ specimens, the same as the other liquid-based methods of cytology. In conclusion, the Liqui-$PREP^{TM}$ and conventional smears showed good correlation, but they have their respective advantages and disadvantages. These results suggest that Liqui-$PREP^{TM}$ can contribute to making the accurate diagnosis with performing breast FNA cytology when it is used along with other methods.

음양곽(淫羊藿) 열수 추출물의 Smad 신호 억제를 통한 간성상세포의 활성 조절

정지윤,민병구,박정아,변성희,조일제,김상찬,Jung, Ji Yun,Min, Byung-Gu,Park, Chung A,Byun, Sung Hui,Cho, Il Je,Kim, Sang Chan 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.3

Objectives : In Traditional Korean Medicine, Epimedium koreanum Nakai has diverse pharmacological activities to treat impotence, forgetfulness, cataract and exophthalmos. Present study investigated anti-fibrogenic effects of E. koreanum water extract (EKE) in hepatic stellate cells (HSCs). Methods : To study anti-fibrogenic effects of EKE, LX-2 cells, a human immortalized HSCs, were pre-treated with $3-300{\mu}g/mL$ of EKE, and then subsequently exposed to 5 ng/mL of transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$). Expression level of ${\alpha}-smooth$ muscle actin was determined by immunoblot analysis. Phosphorylation of Smad, transactivation of Smad, and expression of plasminogen activator inhibitor-1 (PAI-1) were monitored to investigate the effect of EKE on $TGF-{\beta}1-mediated$ signaling pathway. Results : Up to $100{\mu}g/mL$, EKE did not show any cytotoxicity on LX-2 cells. Pre-treatment of EKE ($100{\mu}g/mL$) significantly inhibited ${\alpha}-smooth$ muscle actin expression induced by $TGF-{\beta}1$. In addition, EKE significantly decreased Smad2 and Smad3 phosphorylations, Smad binding element-driven luciferase activity and PAI-1 expression by $TGF-{\beta}1$. Of three flavonoid compounds found in EKE, only quercertin ($30{\mu}M$) attenuated $TGF-{\beta}1-mediated$ PAI-1 expression. Conclusion : These results suggest that EKE has an ability to suppress fibrogenic process in HSCs via inhibition of $TGF-{\beta}1/Smad$ signaling pathway.

‘추론의 인식 양상어’에서 ‘확신의 담화 표지’로의 확장

정지윤(Ji Yoon Jung) 경북대학교 러시아-유라시아 연구소 2023 러시아유라시아연구 Vol.10 No.-

본 논문에서는, 발언에 대한 화자의 심리적 태도를 나타내는 양상에 관한 기존 연구들에서 ‘추론의 인식 양상어’와 ‘확신의 담화 표지’ 개념을 구별하지 못했던 이유는 발화의 ‘인식적 명제 차원’과 ‘화행(표명) 차원’을 구분하지 않았기 때문이며,‘확신의 담화표지’는 ‘추론의 인식 양상어’로부터 은유적으로 확장된 개념임을 밝히고자 한다. 해당 연구는 현대 언어학의 주요한 경향과 원리인 ‘통합적 연구’를 통해 언어 범주적·의미적으로 은유-확장적 개념인 ‘추론’과 ‘확신’에 관하여 기술할 것이다. 근원이 되는 원형을 토대로 다른 개념들이 확장돼 점차 새로운 뜻과 의미를 넓혀가고, 끊임없이 범주를 확장해나가는 방사형 구조를 지니는 언어의 특성을 고려하여 넓은 구조적 가능성에서 ‘추론’과 ‘확신’의 양상어가 보이는 언어 현상이 종합적이고 총체적으로 분석될 것이다. 기존의 양상 연구는‘가능과 필연’의 세계를 주축으로 하는 경우가 대부분이고, ‘믿음(신뢰성; 확신; сommitment)의 세계’ 에 대한 연구는 여전히 부족한 상태이다. 본 연구는 양상 연구가‘믿음 세계’에 관한 연구로 확장되고, 양상의 정의, 즉, ‘화자의 심리적 태도’에 더욱 근접하게 다가가는 발판이 될 것이다. In this paper, I will argue that the existing studies on the aspects that indicate a speaker's psychological attitude towards an utterance have not been able to distinguish between the concepts of 'epistemic modality of inference' and 'discourse marker of commitment' because they do not distinguish between the 'epistemic propositional dimension' and the 'speech act dimension' of an utterance, and that 'discourse marker of commitment' is a metaphorical extension of the concept of 'epistemic modality of inference'. This study will describe the linguistic categorical and semantic metaphorical extensions of the concepts 'inference' and 'commitment' through 'integrative research', a major trend and principle of modern linguistics. Considering the radial structure of language, in which other concepts are expanded on the basis of the underlying archetypes, gradually expanding new meanings and connotations, and constantly expanding categories, the linguistic phenomenon of 'inference' and 'commitmnet' in a wide range of structural possibilities will be analyzed comprehensively and holistically. Existing modal studies are mostly centered on the world of possibility and necessity, and research on the world of belief (reliability; certainty; commitment) is still lacking. This study will serve as a stepping stone for the expansion of modality research into the study of the world of belief, and for moving closer to the definition of modality, i.e. the psychological attitude of the speaker.

프레드니솔론과 타목시펜으로 호전된 경화성 피막성 복막염 3례

정지윤 ( Ji Yoon Jung ),장원익 ( Won Ik Jang ),김의식 ( Eui Sik Kim ),김난희 ( Nan Hee Kim ),정사라 ( Sa Rah Chung ),최대은 ( Dae Eun Choi ),나기량 ( Ki Ryang Na ),이강욱 ( Kang Wook Lee ),신영태 ( Young Tai Shin ) 대한신장학회 2008 춘계학술대회 초록집 Vol.28 No.1

울산 산단지역 PM2.5 중 중금속 노출에 의한 건강위해성평가

정지윤(Ji-Yun Jung),이혜원(Hye-Won Lee),박시현(Si-Hyun Park),이정일(Jeong-Il Lee),윤단기(Dan-Ki Yoon),이철민(Cheol-Min Lee) 한국환경보건학회 2023 한국환경보건학회지 Vol.49 No.2

Background: When particles are absorbed into the human body, they penetrate deep into the lungs and interact with the tissues of the body. Heavy metals in PM2.5 can cause various diseases. The main source of PM2.5 emissions in South Korea’s atmosphere has been surveyed to be places of business. Objectives: The concentration of heavy metals in PM2.5 near the Ulsan Industrial Complex was measured and a health risk assessment was performed for residents near the industrial complex for exposure to heavy metals in PM2.5. Methods: Concentrations of heavy metals in PM2.5 were measured at four measurement sites (Ulsan, Mipo, Onsan, Maegok) near the industrial complexes. Heavy metals were analyzed according to the Air Pollution Monitoring Network Installation and Operation Guidelines presented by the National Institute of Environmental Research. Among them, only five substances (Mn, Ni, As, Cd, Cr6+) were targeted. The risk assessment was conducted on inhalation exposure for five age groups, and the excess cancer risk and hazard quotient were calculated. Results: In the risk assessment of exposure to heavy metals in PM2.5, As, Cd, and Cr6+ exceeded the risk tolerance standard of 10–6 for carcinogenic hazards. The highest hazard levels were observed in Onsan and Mipo industrial complexes. In the case of non-carcinogenic hazards, Mn was identified as exceeding the hazard tolerance of 1, and it showed the highest hazard in the Ulsan Industrial Complex. Conclusions: This study presented a detailed health risk from exposure to heavy metals in PM2.5 by industrial complexes located in Ulsan among five age groups. It is expected to be utilized as the basis for preparing damage control and industrial emission reduction measures against PM2.5 exposure at the Ulsan Industrial Complex.

경도지적장애인의 마음읽기와 언어발달에 관한 연구

정지윤 ( Ji Yun Jung ),김화수 ( Wha Soo Kim ),이근용 ( Kun Youg Rhee ) 대구대학교 한국특수교육문제연구소 2014 특수교육저널 : 이론과 실천 Vol.15 No.3

The purpose of this study is to investigate relationship of mind reading and language development. This study was conducted about 20 adult with mild intellectual disability, 20 general people in the chronological age matched group, and 20 general people in the language age matched group. All theory of mind task performance was compared with mild intellectual disability group (MA), chronological-age (CA) matched group and language-age (LA) matched group. And, language development and theory of mind within MA groups’ correlations were examined. The results of this study were as follows: First, a one way ANOVA was carried out comparing the three groups (MA, CA and LA) with respect to their scores on first-order theory of mind task. The main effect for group was significant. Second, the result of analysis showed that there was statistically significant difference in MA vs. CA groups on the second-order theory of mind task. Third, mild intellectual disabilities within the group in the language test and correlated with theory of mind task performance. The findings are discussed in terms of the specificity of the intellectually disabled group. Therefore the result of this study could give suggestion for intervention and understanding of adult with mild intellectual disability.

黃藥子 메탄올 추출물의 염증억제 효과

정지윤(Ji Yun Jung),이종록(Jong Rok Lee),변성희(Sung Hui Byun),정지욱(Ji Wook Jung),김용한(Yong Han Kim),김상찬(Sang Chan Kim) 한의병리학회 2010 동의생리병리학회지 Vol.24 No.2