L-Ascorbic Acid가 Guinea Pig에서 혈장 지질과산화값, Prostaglandin 생합성, 광용혈, Superoxide Dismutase 및 Catalase활성에 미치는 영향

이정원,이태녕,모수미,이진호,이대형,박수남,이보경,Lee, Joung-Won,Lee, Tae-Young,Mo, Su-Mi,Lee, Jin-Ho,Lee, Dae-Hyung,Park, Soo-Nam,Lee, Bo-Kyung 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.4

L-Ascorbic acid(AsA)를 결핍, 석정, 과잉(적정의 40배)의 세 수준으로 나누어 guinea pig(평균체중 428g, male)에게 5주동안 경구 투여했을때, 적정 및 과잉으로 공급된 AsA는 AsA 결핍군에서 나타난 혈장 및 간조직의 매우 상승된 지질과산화값(TBA값)을 저하시켰으며 이 효과는 과잉공급시 더욱 컸다. 이는 AsA가 항산화작용을 통해 지질 과산화를 억제하였음을 의미한다. 그러나 과량군의 혈장 TBA값은 적정군의 혈장 TBA값 수준에 머물렀는데 이는 과잉의 AsA가 동시에 혈장 TBA값에 포함되어지는 prostaglandin (PG) endoperoxide의 생합성을 촉진했기 때문이다. 그리고 superoxide dismutase(SOD)와 catalase 활성은 AsA결핍시 특히 적혈구에서 증가경향을 보였으나 과잉 공급인 경우엔 별 변화가 없었다. 동물 개개의 SOD활성도는 간조직에서는 TBA값과 (r = .324) 적혈구에서는 용혈수준과 (r = .323) 상관관계 (p < 0.1)가 있었다. AsA의 대량공급이 지질의 과산화반응을 억제한다는 의의 이외에 PG생합성의 촉진수단으로서 중요한 구실을 할 수 있다는 주목할만한 vitamin C의 새로운 생화학적 의의를 시사한다. 또한 AsA의 과량공급은 적혈구의 $^1O_2$에 의한 광용혈을 적정량 공급군에 비해 촉진시키는 결과를 보이고 있다. 이 광용혈 촉진의 생화학적 의의는 PG endoperoxide 생합성 활성화의 결과와 연관성이 있을 것으로 보이며 PG endoperoxides 촉진문제와 더불어 앞으로 검토해야 될 문제로 사료된다. Three levels of L-ascorbic acid(AsA) were orally given to 20 male Hartley guinea pigs weighing average 428 g for five weeks. AsA level was 0, 2.5, and 100 mg per 100g body weight per day in the deficient, the adequate, and the excess group, respectively. Plasma lipid peroxide value(TBA value) of deficient was increased to the highest among the three groups and both the adequate and the excess groups exhibited the lower TBA values, suggesting the antioxidant action of AsA. In the excess of AsA, plasma TBA value was greatly lowered one day after the feeding, but TBA value after 5 weeks was elevated comparable to that of the adequate. Aspirin administrating experiment suggested that this elevation was due to increased. production of prostaglandin(PG) endoperoxides induced by excess AsA. Thus it could be concluded that excess AsA actually inhibited the tissue lipid peroxidation in vivo. The activities of both superoxide dismutase(SOD) and catalase in erythrocytes demonstrated the elevations in the deficient comparing with the auequate, but not any significant variations in the excess. SOD activities were correlated with TBA value in liver (r = .324) and with percent hemolysis in erythrocytes (r = .323). Photohemolysis of erythrocyte was increased not only in the AsA deficient but also in the excess comparing with the adequate. A new finding that the large excess administration of AsA in guinea pig demonstrated a significant stimulation of PG endoperoxide biosynthesis, would suggest an important biochemical implication on vitamin C as an activator of phospholipase $A_2$ and/or PG endoperoxide synthetase. The stimulation of PG biosynthesis might be associated with the enhanced photohemolysis, and this problem needs to be further investigated.

L - Ascorbic Acid 가 Guinea Pig 에서 혈장 지질과산화값 , Prostaglandin 생합성 , 광용혈 , Superoxide Dismutase 및 Catalase 활성에 미치는 영향

이정원,이태녕,모수미,이진호,이대형,박수남,이보경 ( Joung Won Lee,Tae Young Lee,Sumi Mo,Jin Ho Lee,Dae Hyung Lee,Soo Nam Park,Bo Kyung Lee ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.4

Three levels of L-ascorbic acid(AsA) were orally given to 20 male Hartley guinea pigs weighing average 428 g for five weeks. AsA level was 0, 2.5, and 100 mg per 100 g body weight per day in the deficient, the adequate, and the excess group, respectively. Plasma lipid peroxide value(TBA value) of deficient was increased to the highest among the three groups and both the adequate and the excess groups exhibited the lower TBA values, suggesting the antioxidant action of AsA. In the excess of AsA, plasma TBA value was greatly lowered one day after the feeding, but TBA value after 5 weeks was elevated comparable to that of the adequate. Aspirin administrating experiment suggested that this elevation was due to increased. production of prostaglandin(PG) endoperoxides induced by excess AsA. Thus it could be concluded that excess AsA actually inhibited the tissue lipid peroxidation in vivo. The activities of both superoxide dismutase(SOD) and catalase in erythrocytes demonstrated the elevations in the deficient comparing with the auequate, but not any significant variations in the excess. SOD activities were correlated with TBA value in liver (r = .324) and with percent hemolysis in erythrocytes (r = .323). Photohemolysis of erythrocyte was increased not only in the AsA deficient but also in the excess comparing with the adequate. A new finding that the large excess administration of AsA in guinea pig demonstrated a significant stimulation of PG endoperoxide biosynthesis, would suggest an important biochemical implication on vitamin C as an activator of phospholipase A₂ and/or PG endoperoxide synthetase. The stimulation of PG biosynthesis might be associated with the enhanced photohemolysis, and this problem needs to be further investigated.

유사어 벡터 확장을 통한 XML태그의 유사성 검사

이정원,이혜수,이기호,Lee, Jung-Won,Lee, Hye-Soo,Lee, Ki-Ho 한국정보과학회 2002 정보과학회논문지 : 소프트웨어 및 응용 Vol.29 No.9

XML(extensible Markup Language)문서가 웹 문서의 표준으로 자리 매김 할 수 있는 가장 큰 성공요인은 사용자가 문서 타입을 기술할 수 있는 유연성(flexibility)이다. 그러나 XML의 유연성으로 야기되는 문제점은 동일한 의미를 표현하기 위해 XML문서 작성자마다 서로 다른 태그명과 구조를 사용한다는 점이다. 즉 서로 다른 태그 집합, 요소(element), 속성(attribute)에 대한 서로 다른 이름 또는 다른 문서 구조로 인해 다른 태그로 표현된 문서는 서로 다른 부류의 문서로 간주되기 쉽다. 따라서 본 논문은 XML태그에 내재된 의미 정보(semantic information)와 구조 정보(structured information)를 추출하여 의미적으로 최대한 유사한 동의어로 확장하고, XML문서의 확장된 태그간의 의미적 유사도를 비교 분석할 수 있는 개념 기반의 태그 패턴 매처(Tag Pattern Matcher)를 설계 구현하였다. 두 XML문서의 태그간의 의미적 유사도에 가중치를 부여하여 기존의 비구조적인(semi-structured) 문서를 위한 벡터 스페이스 모델(vector space model)을 확장함으로써 두 XML문서가 유사한지를 파악할 수 있다. The success of XML(eXtensible Markup Language) is primarily based on its flexibility : everybody can define the structure of XML documents that represent information in the form he or she desires. XML is so flexible that XML documents cannot be automatically provided with an underlying semantics. Different tag sets, different names for elements or attributes, or different document structures in general mislead the task of classifying and clustering XML documents precisely. In this paper, we design and implement a system that allows checking the semantic-based similarity between XML tags. First, this system extracts the underlying semantics of tags and then expands the synonym set of tags using an WordNet thesaurus and user-defined word library which supports the abbreviation forms and compound words for XML tags. Seconds, considering the relative importance of XML tags in the XML documents, we extend a conventional vector space model which is the most generally used for document model in Information Retrieval field. Using this method, we have been able to check the similarity between XML tags which are represented different tags.

지역사회 획득 소아 요로 감염에서 Extended-Spectrum ${\beta}-Lactamase$ 생성

이정원,신지선,서정완,이미애,이승주,Lee Jung-Won,Shin Jee-Sun,Seo Jeong-Wan,Lee Mi-Ae,Lee Seung-Joo 대한소아신장학회 2004 Childhood kidney diseases Vol.8 No.2

Purpose: Appropriate antibiotic therapy is important in childhood urinary tract infection and the selection of anibiotics is based on antimicrobial sensitivity of Escherichia coli. Extended-Spectrum ${\beta}-Lactamase(ESBL)$ is an enzyme produced by gram-negative bacilli that has the ability to hydrolyse penicillins, broad-spectrum cephalosporin and monobactam. There have been many reports of outbreaks of hospital infection by ESBL-producing organism. However, community-acquired infection with ESBL-producing organism are rare. This study was performed to retrospectively identify the incidence, characteristics and risk factors of ESBL (+) E. coli in community-acquired childhood UTI. Methods: In 288 children admitted in Ewha Womans University Hospital with E. coli UTI from Mar 2001 to February 2003, ESBL was isolated. ESBL was confirmed by the utilization of an automatized machine(Vitek GNS 433 card) using liquid medium dilution method according to National Committee for Clinical Laboratory Standard. The clinical characteristics, risk factors, antimicrobial resistance and treatment effectiveness were compared with ESBL(-) E. coli UTI. Results: Of 288 E. coli isolates, 31(10.8%) produced ESBL and 93.5%(29/31) occurred in infants younger than 6 month of age(P<0.01). No significant differences were noted in prior antibiotic use, prior admission history and underlying urogenital anomaly. Antimicrobial resistance was significantly higher in ESBL(+) E. coli compared with control patients (P<0.05). Although ceftriaxone showed 100% resistance in ESBL(+) E. coli, bacteriologic sterilization rate after ceftriaxone therapy was higher(96.8%). However, the recurrence rate of febrile UTI within 6 months was higher(25.8%) than control patients(6.6%). Conclusion: Epidemiologic study is required to find out any new risk factors of community-acquired ESBL(+) E. coli UTI and changes in selection of empirical antibiotics should be considered.

송전철탑 Compact화에 따른 전기환경 영향 연구

이정원,이원교,이동일,Lee, Jung-Won,Lee, Won-Kyo,Lee, Dong-Il 한국전기전자재료학회 2010 전기전자재료학회논문지 Vol.23 No.8

The continuous increase demand for electric power leads to the additional construction of transmission facilities, but it is not easy to acquire right-of-way for transmission facilities. Therefor, there is a need for compact tower that can be built on a narrow right-of-way the compact tower with polymer insulation arm is a solution. It can be upgrading conventional 154 kV transmission line voltages to 345 kV levels. However transmission voltage is increasing, environment interference (corona noise, radio interference, etc.) will occur gradually. This environment interference is depending on the electrical clearances of tower and configuration of conductors. Therefore the analysis of the factors of environmental interference is necessary in order to upgrading transmission voltage. This paper presents the design factor of a compact tower to meet the environmental interference standard.

하수오환(何首烏丸)이 노화(老化)유발 백서(白鼠)의 항산화능에 미치는 영향

이정원,이송실,백진웅,이상재,김광호,Lee Jeong-Won,Lee Song-Shil,Baek Jin-Woong,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2004 대한예방한의학회지 Vol.8 No.1

Hasuohwan(何首烏丸) composed of Polygonum multiflorum Thunb and some medical herbs are known as formula of senescence delay effect. The aim of this study is to investigate the effect of Hasuohwan(何首烏丸) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS) in rat plasma and liver, Superoxide dismutase(SOD), Glutathione peroxidase(GSH-px), Catalase(CAT) in rat erythrocyte and liver. Rats were sacrificed and TBARS was measured in rat plasma and liver. SOD, GSH-px and CAT were measured in rat erythrocytes and liver. TBARS in plasma concentrations of HSO group was significantly lower than those of control group. RBC and liver GSH-px activities of HSO group were significantly higher than those of control group. According to above results, it is considered that Hasuohwan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat. Therefore, Hsuohwan is considered in effective of senescence delay.

한의과대학 학생의 직업가치가 전공몰입, 전공만족에 미치는 영향

이정원,김경철,Lee, Jeong Won,Kim, Gyeong Chul 대한예방한의학회 2020 대한예방한의학회지 Vol.24 No.1

Objective : The purpose of this study is to determine the Work value of Korean medicine college students and check how the Work value of college students affects major commitment and major satisfaction. Method : The data for this study were collected through a self-administered survey to 207 subject from students enrolled in the University in B city. Frequency analysis, repeated measures ANOVA, multiple regression analysis were used for the statistical analysis. Results : As a result of analyzing the occupational value of students enrolled in the College of Korean Medicine, the pursuit of professionality and interest was the highest, the pursuit of achievement was the next highest, and the pursuit of diversity and change was the lowest. As a result of the multiple regression analysis, pursuit of achievement and professionality had a positive(+) effect, and pursuit of ease had a negative(-) effect on the major commitment. Also, the pursuit of professionality and interest had a positive(+) effect, and pursuit of ease had negative(-) effect on major satisfaction. Conclusion : In order to increase students' major commitment and satisfaction, it is necessary to make efforts to compose the programs and educational contents that can satisfy these values. In addition, the efforts of universities and professors are needed to help students establish the correct their work value.

Powder blasting을 이용한 Fused silica glass의 마이크로 채널 가공 및 특성 평가에 관한 연구

이정원,김태민,신봉철,Lee, Jung-Won,Kim, Tae-Min,Shin, Bong-Cheol 한국금형공학회 2020 한국금형공학회지 Vol.14 No.1

Recently, due to the development of MEMS technology, researches for the production of effective micro structures and shapes have been actively conducted. However, the process technology based on chemical etching has a number of problems such as environmental pollution and time problems due to multi-process. Various processes to cope with this process are being studied, and one of the mechanical etching processes is the powder blasting process. This process is a method of spraying fine particles, which has the advantage of being an effective process in manufacturing hard brittle materials. However, it is also a process that adversely affects the material surface roughness and material properties due to the impact of the injection of fine particles. In this study, after fabricating micro-channels in fused silica glass with excellent optical properties among the hard brittle materials, we used the nano indentation system to analyze the micro parts using nano-particles as well as machinability and surface roughness analysis of the processed surface. The analysis was performed for the effective processing of powder blasting.

초음파 보조하에 시행한 치골상부 방광천자의 유용성

이정원,박소은,조수진,유은선,김혜순,이승주,Lee Jung Won,Park Soeun,Cho Su Jin,Yoo Eun Sun,Kim Hae Soon,Lee Seoung Joo 대한소아신장학회 2002 Childhood kidney diseases Vol.6 No.1

Purpose: Suprapubic bladder aspiration(SBA) of urine is the most reliable method to obtain urine avoiding contamination in non-toilet trained infants. Ultrasonography is a useful tool for guiding the anatomic location as well as for direct visualization during procedure. We evaluated the success rate and complication of ultrasound(US) assisted SBA Methods. Sixty infants who visited Ewha Womans University Mokdong Hospital, with suspected urinary tract infection were randomly divided into the US assisted (n=32) and blind SBA(control, n=28) group. In US assisted SBA group, the anteroposterior(AP), transverse, and sagittal diameters and the volume of the bladder were measured. In the blind SBA group, urine was blindly aspirated when the urinary bladder was palpated at the suprapubic area. The rate of successful urine aspiration, the number of attempts until successful aspiration, aspirated urine volume were compared between the two groups. Results: The success rate was $100\%$(32/32) in the US assisted group, which was significantly higher than $85.7\%$(24/28) of the control group (P<0.05). The aspirated urine volume in the US assisted group was $7.4{\pm}3.7\;mL$, which was significantly higher than $4.5{\pm}3.4\;mL$ of the control group (P<0.05), The diameters and volume of bladder in successful aspiration were $2.1{\pm}0.7\;cm$ in AP diameter, $3.1{\pm}0.6\;cm$ in transverse diameter, $4.2{\pm}1.0\;cm$ in sagittal diameter and $15.2{\pm}10.4\;mL$ in volume, which were significantly higher than those ($1.7{\pm}0.3\;cm,\;1.7{\pm}0.3\;cm,\;1.8{\pm}0.7\;cm,\;2.4{\pm]0.6\;cm,\;3.9{\pm}2.5$) of the control group (P<0.05) The correlations between the AP(r=0.78), transverse (r=0.72), sagittal(r=0.91) diameter and bladder volume were significant (P<0.05). SBA was $100\%$ successful in the AP diameter >3 cm, transverse diameter >4 cm, depth >4 cm and bladder volume >5 mL. Conclusion: US assistance can significantly improve the success rate of SBA in infant with suspected urinary tract infection. (J Korean Soc Pediatr Nephrol 2002 ; 6 : 68-74)

가습 입자를 활용한 고효율 난방 시스템 개발

이정원,홍경보,Lee, Jeong-Won,Hong, Kyung-Bo 한국금형공학회 2020 한국금형공학회지 Vol.14 No.4

Products for heating indoors in low temperature and dry winter are largely divided into products using fossil fuels and products using electricity. The fossil fuels can warm the entire space by convection, but there is a high risk of fire and the frequent ventilation due to the increase in carbon monoxide and carbon dioxide. Heaters using electricity are mainly used because they are convenient to use and are cheap. However, these products can not efficiently warm the air because they use radiation energy. In other words, only the front part exposed to the heater is warm, and the rear part has no heating effect at all. Also, because it emits a large amount of light, fatigue of the eyes is very high. Another problem is that when using electric heaters, the room tends to be dry by high heat. Indoor humidity maintenance is a very important factor in the prevention and treatment of respiratory diseases. Especially, it is essential for health care for infants, bronchial organs and people with weak respiratory because humidity is low in winter. In this study, we conducted a study to develop a product that can improve heating efficiency while maintaining proper indoor humidity by combining heat energy and moisture particles. The concept of humidification and heating at the same time, moisture particles generated in the humidifier pass through the heater, include thermal energy, and the moisture particles with thermal energy are diffused into the space by forced convection, thereby warming the entire space. In addition, the heating time is shortened as the feeling temperature is increased with the high relative humidity, and this has the effect that the heating cost in winter is reduced.