방사선 이용 벼 돌연변이 계통 선발 및 농경 형질조사

이인석,김동섭,이상재,송회섭,임용표,이영일,Lee, In-Sok,Kim, Dong-Sub,Lee, Sang-Jae,Song, Hi-Sub,Lim, Yong-Pyo,Lee, Young-Il 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1

Radiation technique in agriculture was initiated to develop mutant rice. Seeds of Daechungbyeo rice were irradiated with 250 Gy gamma ray for the purpose of inducing and selecting rice variants. Some quantitative traits of the variants in M$_{8}$ generation were evaluated and RAPD analysis was carried out. Variants showed a wider range of agronomic characteristics in both a positive and a negative direction compared with their original variety. The new mutants were characterized by an increased or decreased in plant height, lodging resistance and shorter panicle. RAPD analysis showed that polymorphic bands were presented in most of the primers. In comparison with the original variety, variants were classified into four groups through UPGMA analysis. Among mutants no. 91, 139, 140 and 141 was ranked as salt tolerance and the proline content of these mutants was more increased than that of original variety. The lines of 139, 140 and 141 had the highest genetic distance as compared to original variety in the dendrogram. It is expected that such variants will be useful not only for studying molecular genetics but also for breeding research and genetic analysis.s. 본 실험은 방사선 처리에서 유기된 벼 변이계통의 몇 가지 농경 형질 분석 및 RAPD 분석을 통하여 방사선 이용 돌연변이 육종연구를 위한 기초적인 자료를 얻기 위하여 실시하였다. 1. 방사선에 의해 선발된 변이 계통의 초장, 수장 및 내도복성 형질은 모품종과 비교하여 정 (+)및 부 (-)의 방향으로 작용하였다. 2. RAPD 분석에 의해 계통 간 polymorphic band를 관찰할 수 있었고, UPGMA에 의해 변이계통를 4 groups으로 분류할 수 있었다. 3. 변이체들 중 계통 91, 139, 140 및 141은 내염성 형질을 나타냈고, 유리 proline 함량이 모품종보다 유의성 있는 증가를 나타내었다. 4. 139, 140 및 141 계통은 dendrogram에서 같은 그룹을 이루었고 모품종과 가장 먼 유전적 거리를 나타내었다. 5. 이러한 계통들은 육종 및 분자유전 연구에 유용한 재료로 이용될 수 있다.

방사선 처리에 의해 유도된 돌연변이 벼의 주요 특징

이인석,김동섭,최수련,송희섭,이상재,임용표,이영일,Lee, In-Sok,Kim, Dong-Sup,Choi, Su-Ryun,Song, Hi-Sup,Lee, Sang-Jae,Lim, Yong-Pyo,Lee, Young-Il 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3

Radiation technique has been used to develope mutant rice. Suwon 345 rice seeds were irradiated with 250 Gy gamma ray. Morphological characteristics of the variants in M$_{8}$ generation were observed and random amplified polymorphic DNA(RAPD) analysis was carried out. Plant height, panicle length, 1,000 grain weight and lodging were very different in mutants compared with donor cultivar. RAPD analysis showed that polymorphic bands were presented in several primers of the mutants. In comparison with original variety, variants were classified into four group through UPGMA analysis. A group has mutation trait in panicle length, B group in plant height and C group in 1,000 grain weight. Among mutants, no. 46 and 147 was ranked as salt tolerance and the malonaldehyde content of these mutants was more increased than that of original variety. Valuable mutants obtained will be useful for developing new cultivars and for studing gene function in molecular level.l.

HPLC를 이용한 지역 농산물의 비타민 B₆ 함량 분석

최소라 ( So-ra Choi ),송은주 ( Eun-ju Song ),송영은 ( Young-eun Song ),최민경 ( Min-kyung Choi ),한현아 ( Hyun-ah Han ),이인석 ( In-sok Lee ),신소희 ( So-hee Shin ),이기권 ( Ki-kwon Lee ),최용민 ( Young-min Choi ),김행란 ( Haeng-r 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.4

Contents of water soluble vitamin B6 in a total of 62 agricultural products cultivated in local areas in Korea were analyzed by high performance liquid chromatography with fluorescence detector (HPLC/FLD). To verify the method of vitamin B6 analysis, a quality control chart was formulated with in-house control using a mixture of broccoli and shiitake mushrooms. Among cereals, high content of vitamin B6 measured 234.3~260.3 μg/100 g in dried mung bean and soybean. Vitamin B6 content of non-glutinous and glutinous black rice measured 105.0 μg/100 g and 129.7 μg/100 g, respectively. In vegetables, high content of vitamin B6 were measured in passion fruit (104.3 μg/100 g), gat (55.7~84.3 μg/100 g), gomchwi (31.3~88.0 μg/100 g) and garlic (72.7~98.3 μg/100 g). Among fruits, gold kiwi 'Zespri' and green kiwi 'Hayward' revealed high vitamin B6 content of 116.3 μg/100 g and 78.7 μg/100 g, respectively. In persimmons, daebongsi had high vitamin B6 content (36.0~72.7 μg/100 g) than bansi and sweet persimmon. Vitamin B₆ content in dried jujube and persimmon increased more than 86.7 μg/100 g compared to fresh materials. Among specialty crops, green tea powder (64.7~251.0 μg/100 g) and sansuyu (172.3 μg/100 g) revealed high content. Of mushrooms, vitamin B6 content of Sparassis crispa (139.3 μg/100 g) was the highest. Vitamin B6 content information of agricultural products in local areas in Korea collected from this experiment will be used as valuable preliminary data for grasp national nutritional status.

방사선을 이용한 내염성 계통의 기내선발 및 특징

이인석,김동섭,현도윤,임용표,이영일,Lee, In-Sok,Kim, Dong-Sub,Hyun, Do-Yoon,Lim, Yong-Pyo,Lee, Young-Il 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4

1.5% NaCl이 함유된 배지에 캘러스를 치상하여 방사선을 처리한 결과 무처리구보다 처리구 (30, 50 Gy)에서 캘러스 생존율 및 재분화율이 증가하여 내염성 캘러스를 선발하는데 방사선의 적용이 효과적임을 알 수 있었고, 내염성 캘러스에서 재분화된 M$_3$세대 종자에서 내염성 계통들은 모품종보다 초장, 근장 및 근수의 생육이 우수하여 이러한 계통은 내염성 연구를 위한 유용한 유전자원으로 이용할 수 있을 것으로 생각된다. 또한 RAPD 기술은 대조구와 내염성 캘러스에서 재분화된 계통을 구분하는데 유용한 기술임을 알 수 있었다. The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.

고품질 볏짚 사일리지 제조를 위한 다양한 유산균주 평가

이인석,이송이,최민경,강찬호,김정만,Lee, In-Sok,Lee, Song-Yee,Choi, Min-Kyung,Kang, Chan-Ho,Kim, Jeong-Man 한국작물학회 2018 한국작물학회지 Vol.63 No.2

현재 국내에는 C사에서 생산하는 한 종의 사일리지 제조 유산균이 있다. 본 연구는 이를 대체할 균주를 선발하기 위해서 사일리지의 특성과 사료가치를 분석하였다. C사의 유산균(CMRT, 비교균주)보다 우수한 균주를 선발하기 위해 김치에서 발견한 유산균 4종(Lactobacillus. leuconostoc mesenteroides M17, L. sakei C11, M5, SP2)을 이용하였다. 성장속도를 측정할 수 있는 흡광도는 L. sakei C11이 가장 높았고, 향은 M17이 제조 20일부터 비교균주와 유사하였다. M17의 pH는 비교 균주보다 낮았다. 단백질 함량은 M17의 사일리지에서 비교균주의 사일리지보다 유의성 있는 증가를 보였다. 유기산 중 초산과 낙산은 검출되지 않았고 젖산은 4개의 선발균주가 비교균주보다 높았다. 4개의 선발 균주 ADF와 NDF의 함량은 비교균주와 비교시 각각11~24% 및 13~20% 낮아 유의성(p < 0.05)있는 차이를 보였다. 비교균주 TDN은 평균 63.44%로 4개의 선발균주의 TDN 값(66.05~69.65)보다 낮은 유의성(p < 0.05)있는 감소를 보였다. 한편 상대사료가치(RFV)는 비교균주 사일리지에서 107.35로 2등급(RFV 124~103)에 해당하는 품질이었으나 4개의 선발 균주 사일리지는 특(RFV 151 이상)~1등급(RFV 125~150)에 해당하여 선발 균주의 사일리지가 품질이 우수한 것으로 조사되었다. 이상의 결과를 종합하여 볼 때, 선발 균주 중 M17에 의해 제조된 사일리지는 비교균주(CMRT) 사일리지 보다 pH, ADF 및 NDF가 낮고, 단백질, 젖산 및 상대사료가치가 높아 고품질의 사일리지 생산이 가능할 것으로 판단되었다.

전북지역 간척지에서 최고품질 벼 품종의 작물학적·이화학적 특성 비교 및 선발

최창학,김갑철,이덕렬,조승현,조대호,이송이,이인석,Choi, Chang-Hak,Kim, Kab-Cheol,Lee, Deok-Ryeol,Cho, Seung-Hyun,Cho, Dae-Ho,Lee, Song-Yi,Lee, In-Sok 한국작물학회 2018 한국작물학회지 Vol.63 No.3

The main objective of this study was to identify the best quality rice for cultivation on reclaimed land in Jeollabukdo-Province based on yield, head rice ratio, protein content, amylose content, and Toyo taste value evaluations. Seven varieties of rice were grown for two years on the reclaimed land. The Sukwang variety had the earliest heading date. The tiller number for the Younghojinmi variety was 19 on average, and its variation in tiller number was the lowest, which showed that the safety for cultivation of the variety was high on the reclaimed land with respect to that of the other varieties. The culm and panicle lengths of the Sukwang and Hyunpum varieties were the highest at 81.8 and 21.3 cm, respectively. Shindongjin produced the highest thousand grain weight at 27.7 g, whereas Haepum produced the lowest at 22.3 g. The number of grains per panicle for 7 all varieties was statistically equal for both years. The yield order for the varieties was Shindongjin (590) > Hopum (575) > Younghojinmi (552) > Sukwang (551) > Hopum (543) > Mipum (534) > Haepum (498 kg/10a), which showed that there was no significant difference in average yield between the varieties when both years are taken into consideration. However, the annual difference in Haepum yield between 2015 and 2016 was the highest at 108 kg/10a, indicating low cultivation safety. The head rice ratio of Younghojinmi was higher than for the other cultivars in both 2015 and 2016. However, there was no significant difference between the 2 years when the averages for all varieties were analyzed. There was was a considerable difference in protein and amylose content between the varieties in the two years, although this was not apparent in the results when the average over 2 years for each variety was calculated. There was no significant difference between the two years with respect to the average head rice ratio, and the protein and amylose contents of the seven varieties. The Toyo taste values for the Sukwang and Mipum varieties were higher than for the other varieties. Based on the principle characteristics, such as Toyo value, the Sukwang variety is a suitable substitute for Shindongjin, which has been previously used to produce high-quality rice on the reclaimed land.

벼멸구 저항성 유전자와 내염성과의 연관

양대화,김진홍,위승곤,백명화,임상용,이인석,이규성,이명철,임용표,정병엽,김재성,Yang Dae Hwa,Kim Jin-Hong,Wi Seung Gon,Baek Myung-Hwa,Lim Sang Yong,Lee In Sok,Lee Kyu-Seong,Lee Myung Chul,Lim Yong-Pyo,Chung Byung Yeoup,Kim Jae-Sung 한국생명과학회 2005 생명과학회지 Vol.15 No.1

Using two japonica rice cultivars (Ilpumbyeo and Sanghaehyanghyella), which are distinguishable by the brown planthopper (BHP) resistance maker (R208), a relationship between the BPH resistance gene (Os-Bil) and salt-tolerance was investigated. To do this, changes in the expression level of Os-Bil by the salt stress were quantified by the real-time PCR in the two cultivars, and compared with those in other two indica rice cultivars (Pokkali and IR29). In Ilpumbyeo, the expression level of Os-Bil decreased by the treatments of 50 and 200 mM NaCl in a concentration-dependent manner, and in Sanghaehyanghyella it rather increased slightly at 50 mM but decreased drastically at 200 mM. Comparably, IR29, a salt-sensitive cultivar, showed a reduction of the Os-Bil gene expression after the treatment of 100 mM NaCl, but Pokkali, a salt-tolerance cultivar, rather increased about two times in the level of Os-Bil transcripts. These results suggest that the BPH resistance gene may involve in the difference in the salt-tolerance at least between the two indica rice cultivars. 벼멸구(Nilapawata lugens Stal.)에 대한 저항성 마커 (R208)에서 확실한 차이를 보인 두 자포니카 품종(일품벼과 상해향혈나)을 대상으로 벼멸구 저항성 유전자(Os-Bil)와 내염성과의 관련성을 조사하였다. 이를 위해 두 품종에서 염처리에 의한 Os-Bil 발현량의 변화를 real-time PCR을 이용해 정량화 하였으며, 인디카 두 품종(Pokkali와 IR29)의 결과와 비교하였다. 일품벼는 50, 200 mM NaCl 처리에서 Os-Bil 유전자의 발현량이 농도 의존적으로 감소하였으며, 상해향혈나는 50mM에서만 약간 증가하고 200mM에서는 크게 감소하였다. 비교해서, 내염성인 Pokkali는 Os-Bil의 발현량이 100 mM의 NaCl 처리에 의해 약 2배 증가하였으나 감수성인 IR29는 같은 농도에서 발현량이 감소하였다. 이러한 결과들은 벼멸구 저항성 유전자(Os-Bil)가 적어도 인디카 품종들의 내염성 차이에 관여함을 의미하는 것으로 보인다.

여교잡을 통한 케나프 바이오매스 우수 계통 선발

이인석(In-Sok Lee),강찬호(Chan-Ho Kang),이진재(Jin-Jae Lee),김희준(Hee-Jun Kim) 한국육종학회 2021 한국육종학회지 Vol.53 No.3

Multipurpose utilization, including as rope, pulp, and filtration media, is what makes kenaf (Hibiscus cannabinus L.) special. Thisexperiment was conducted to select elite pedigrees with high biomass production through backcrosses. The agronomic performance, fuelcharacteristics, and histological analysis of the elite lines were investigated at the BC1F2 generation in comparison with the Control. Significantdifferences were observed in 12 evaluations of variety and pedigrees. Five plants, including the two Controls (Jangdae and Hongma300) andthree pedigrees, showed a large difference in the number of flowering days from July 5 to September 30. The plant height of BC100-10(447 cm) was the highest among the genotypes. Regarding branch number, BC100-15 showed the best performance with 29 branches. Stemdry weight was found to be the lowest in Jangdea at 792 g, whereas BC100-17 showed the highest dry weight. Hongma did not set seedsowing to late flowering. However, with 34 g/plant on average, three elite lines had more seeds than Jangdae (16 g/plant), which are vulnerableto decay owing to viviparous germination. In the fuel characteristics analysis, the heating value of the three elite lines ranged from 4,510to 4,930 kcal/kg, which was much higher than that of the two Controls (4,320 kcal/kg). The ash content analysis indicated the lowest valueat 3.2% for the line BC100-10, which was similar to Korea’s 3rd-grade. Based on the histological analysis of the stem, the pedigrees wereclose to Hongma. Taken together, these three elite lines would be useful as genetic resources for high biomass production.

아산시 지역에서 과민성 장 증후군의 유병률: 표본인구 설문조사

조영석 ( Young Seok Cho ),최명규 ( Myung Gyu Choi ),신석준 ( Seok Jun Shin ),추교영 ( Kyo Young Choo ),정우철 ( Woo Chul Chung ),이인석 ( In Seok Lee ),김상우 ( Sang Woo Kim ),최황 ( Hwang Choi ),한석원 ( Sok Won Han ),정인식 ( I 대한소화기기능성질환·운동학회 2004 Journal of Neurogastroenterology and Motility (JNM Vol.10 No.1

목적: 우리나라의 과민성 장 증후군과 배변증상의 유병률을 조사하고, 다른 진단 기준을 적용하였을 때 유병률에 차이가 있는 지를 알아보기 위하여 지역사회 주민을 대상으로 무작위 표본추출조사를 시행하였다. 대상 및 방법: 충청남도 아산시 보건소와 협조하여 11개 면과 2개동을 무작위로 선정한 후, 인구에 비례하여 18세 이상 70세 미만의 남녀 2,024명을 무작위로 표본추출하였다. 조사원이 대상가구를 직접 방문하여 배변증상을 설문지로 조사하였다. 결과: 3회 방문에 부재 중이거나 제외기준에 속한 주민을 제외하고, 1,417명(남 762명, 평균 44±14세, 여 655명, 평균 43±15세) 이 설문지를 완료하였다. 로마기준 II에 의한 과민성 장 증후군의 유병률은 2.2% (95% 신뢰구간 [95% CI], 1.4~3.0)이었고, 로마기준 I으로는 2.8% ([95% CI], 1.9~3.7)였다. 복통과 Manning 기준 중 2가지 이상을 만족하는 과민성 장 증후군의 유병률은 3.6% ([95% CI], 2.6~4.6)였다. Manning 기준과 로마기준 I, 그리고 Manning 기준과 로마기준 II간에는 각각 moderate agreement를 나타내었다(κ=0.48; p<0.001), (κ=0.50; p<0.001). 로마기준 I과 로마기준 II간에는 substantial agreement를 나타내어(κ=0.66; p<0.001) 세 가지 진단기준 중 로마기준 I과 로마기준 II의 경우에 일치의 정도가 가장 높았다. 결론: 로마기준 II를 적용하여 표본추출 설문조사에 의한 우리나라의 과민성 장 증후군의 유병률은 2.2%이며, 적용된 진단기준에 따라 차이가 있었다. Backgr ound/Aims: Irritable bowel syndrome (IBS) is common in Caucasians and Japanese, but its epidemiology has not been studied in random Korean populations. It has been suggested that the variation in the prevalence of IBS may be due to the application of different diagnostic criteria. New criteria for IBS have been proposed (Rome II). The aim of this study was to estimate the prevalence of IBS in a random population in Asan city, Korea by using the Bowel Symptom Questionnaire (BSQ), which was based on the multinational, diagnostic, Rome II criteria for functional bowel disorders, as a measure of GI symptoms and to compare diagnostic criteria. Methods: A cross-sectional survey, using a reliable and valid questionnaire based on the fulfillment of the Rome II criteria was performed in a random population in Asan city, Korea, on residents aged 18~69 yr. The response rate was 78.4% (n=1417). All respondents were interviewed at either their homes or offices by a team of interviewers. Results: The prevalence for BS according to the Rome II, Rome I, and Manning criteria was 2.2% (95% confidence interval [CI], 1.4~3.0), 2.8% (95% CI, 1.9~3.7), and 3.6% (95% CI, 2.6~4.6), respectively. There was moderate agreement between the Manning and Rome II criteria (κ=0.48; p<0.001), and between the Manning and Rome II criteria (κ=0.50; p<0.001), but substantial agreement between the Rome I and Rome II criteria (κ=0.66; p<0.001). The prevalence for abdominal bloating was 4.0% (95% CI, 3.0~5.1), for diarrhea was 0.8% (95% CI, 0.3~1.2), and for constipation was 2.6% (95% CI, 1.8~3.5). Conclusions: The prevalence of IBS based on the Rome II criteria was 2.2%. The prevalence of IBS varied substantially depending on the specific definition of IBS used. (Kor J Neur ogastr oenterol Motil 2004;1:49-56)

다양한 건조방법에 따른 블랙베리 분말의 품질 특성

최소라 ( So-ra Choi ),송은주 ( Eun-ju Song ),송영은 ( Young-eun Song ),최민경 ( Min-kyung Choi ),한현아 ( Hyun-ah Han ),이인석 ( In-sok Lee ),신소희 ( So-hee Shin ),이기권 ( Ki-kwon Lee ),김은주 ( Eun-ju Kim ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.3

This experiment was carried out to enhance the availability of blackberry. Since it is difficult to use blackberry as a fresh fruit, we investigated the quality characteristics of blackberry powder obtained by various drying methods (freeze drying and hot-air drying at 40~80℃). The L- and b-values of freeze-dried powder was higher than hot-air dried powder. The pH (3.2) was lowest and the acidity (14.4%) was highest in freeze-dried powder. In freeze drying, the brix degree was 65.7 °Bx, but it increased from 54.7 °Bx to 68.5 °Bx with increasing temperature during hot air drying. The total polyphenol and flavonoids contents were the highest in freeze-dried powder, at 9.3 and 6.2 mg/g, respectively. The levels increased as temperature increased in hot air drying. Anthocyanin content in freeze-dried powder was 8.51 mg/g, while it sharply decreased to 1.17~2.45 mg/g in hot-air drying. Vitamin C content in freeze drying (979.4 μg/g) was higher than that in hot-air drying (48.3~303.2 μg/g). The sample concentration required for 50% reduction of DPPH free radical scavenging (RC50) was 79.7 μg/mL in freeze drying, and showed high antioxidant activity. Also it decreased from 122.4 μg/mL to 87.7 μg/mL with temperature increase during hot air drying. We therefore conclude from the above results that freeze drying is more suitable for the production of blackberry powder, because this method showed high value of chromaticity, total polyphenol, flavonoid, anthocyanin content, vitamin C and antioxidant activity.