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한국재래산양 태자 및 신생양 중뇌에서 GFAP 면역반응세포의 분포에 관한 형태학적 연구
송치원(Chi-Won Song),이강이(Kang-Iee Lee),변명우(Myung-Woo Byun),육홍선(Hong-Sun Yook),김정재(Jeong-Jae Kim),신상태(Sang-Tae Shin),이경열(Kyoung-Youl Lee),김무강(Moo-Kang Kim) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.4
한국재래산양의 임신 30, 45, 60, 90, 105, 120일령 태자 및 신생양의 중뇌를 대상으로 면역조직화학적 방법을 이용하여 GFAP 면역반응세포를 형태학적으로 연구하여 아래와 같은 결과를 얻었다. 1. GFAP 면역반응세포는 임신 45일령 태자에서부터 관찰할 수 있었으며, 이들은 무극형이었다. 2. 임신 60일령 태자에서는 뭇극형 면역반응세포가 관찰되었으며, 임신 90일령 태자에서는 중뇌수도관주위구역에 뭇극형면역반응세포가 관찰되었다. 3. 방사형아교세포의 돌기형태의 변화는 무극형면역반응세포가 두극형세포로 변화하였고, 두극형세포는 홑극형으로 변한 다음 뭇극형면역반응세포로 변형되고 있었다. 4. 면역반응을 나타낸 두극형면역반응세포들의 돌기들은 뇌실에서 연질막으로 뻗고 있었으며 연질막부위에서 종말발로 확장되어 있었다. 5. 한 시야당 GFAP 면역반응세포의 수는 임신 45일령 태자에서 임신 90일령 태자까지는 증가하였고, 임신 90일령 태자 에서 105일령 태자까지는 감소하였으며 임신 120일령 태자에서는 그 수가 105일령 태자보다 약간 증가한 양상을 나타내었다. 6. GFAP 면역반응세포의 면역반응은 임신 95일령 태자에서 120일령 태자까지 감소하는 양상이 관찰되었지만 대부분의 연질막부위와 뇌실부위에서는 강한 면역반응을 나타내고 있었다. 이러한 결과들은 면역반응세포가 중뇌수도관주위구역보다 중뇌부위에서 더 빠르게 발달하고 있었다. GFAP (Glial Fibrillary Acidic Protein) was one of the intermediate filament group and used as an astrocyte marker. The numerous studies about GFAP immunoreactive cell’s distribution were investigated for fetus, neonate and aged brains. There are several reports about that GFAP immunoreactive cells were appeared at early fetus and after birth. In cases of mammalian fetus radial glia cells migrated toward pial surface at early stage and revealed GFAP immunoreactivity by the immunostain. But in cases of rodents, they migrated at late gestation or after birth. This study, the GFAP immunoreactive cells’ localizations and distribution in the fetuses (the 30 th, 45 th, 60 th, 90 th, 105 th, 120 th of gestation) and neonate mesencephalon of korean native goat were investigated by immunohistochemistry (ABC method). The results obtained in this study were summarized as followings; 1. Multipolar astrocytes at 60 days of gestation were found in midbrain, in 90 days of gestation were found in cerebral aqueduct. 2. Radial glial cell presented 60 days of gestation and process of GFAP immunoreaction was to stretch out from ventricular to pia mater and nonpolar immunoreactive cell was transformed to bipolar, monopolar and multipolar immunoreactive cell. 3. The number of GFAP immunoreactive cells of field were gradually decreased from 90 days of gestation till 105 days of gestation. But in 120 days of gestation and newborn were slightly increased. 4. Immunoreactivity of GFAP immunoreactive cells were gradually decreased from 95 days of gestation till 120 days of gestatioin. These results were suggested that radial glial cell of midbrain developed very earlier than that of cerebral aqueduct. However, cerebral aqueduct developed lately than that of midbrain, but faster developing than other.
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한국산 야생등줄쥐 (Apodemus Agrarius coreae)의 대뇌피질과 줄무늬체에서 Neuropeptide Y 면역반응신경세포의 분포
이경열(Kyoung-Youl Lee),박일권(Il-Kwon Park),송치원(Chi-Won Song),권효정(Hyo-Jung Kwon),박미선(Mi-Sun Park),김무강, 정영길(Young-Gil Jung),이강이(Kang-Iee Lee),이철호(Chun-Ho Lee),현병화(Byung-Hwa Hyun),최농훈(Nong-Hun Choe) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.2
Neuropeptide Y (NPY)는 돼지의 뇌에서 처음 분리 추출된 이후 여러학자들에 의해 여러동물의 뇌에 존재함이 밝혀졌으 며 이런 NPY는 신경조절물질 또는 신경활성물질에 관여하는 것으로 있려져 있다. 본 연구에서는 한국산 야생등줄쥐 (apodemus agrarius cereae)의 대뇌피질과 줄무늬체에서의 NPY면역반응신경세포체의 분포를 관찰하고자 면역조직화학염 색을 실시하여 관찰하였던 바 다음과 같은 결론을 얻었다. 1. 야생등줄쥐의 대뇌피질에서 NPY면역반응신경세포체가 모든 층에서 출현하였으며 특히 V층과 VI층에 주로 분포하 였고 I층과 VI층에서는 드물게 나타났다. 2. 대뇌피질에서 NPY면역반응신경세포체의 모양은 타원형, 난원형, 뭇각형 등 다양하게 존재하였고 I층과 II층에서는 원 형과 타원형이 많았으며 V층과 VI층에서는 뭇각형이 많았다. 세포돌기는 두극형 또는 뭇극형으로 존재하였는데 그 세포돌기의 주행방향이 I층과 II층에서는 연질막 (pia mater)과 평행으로 III층에서 두극형의 세포는 수직방향으로 그 돌기가 뻗어 있었으며 VI층에서는 뇌량과 평행하게 주행하고 있었다. 세포체의 크기는 중간크기 (10~20 μm)의 세포가 대부분이고 20 μm 이상인 세포는 드물게 관찰되었다. 3. 대뇌피질의 부위별 분포는 후각뇌주위피질에서 그 수가 가장 많이 나타났고 두정피질에서 그 다음으로 많이 나타났 으며 팽대뒤피질에서는 가장 적은 것으로 나타났다. 그러나 섬피질에서는 갯수는 적으나 그 밀집도가 높았다. 두정피 질부위에서는 대개 III-VI층에 주로 분포하였고 전두피질에서는 다른 부위보다 II층에 많은 세포체가 존재하였다. 4. 줄무늬체에서의 NPY면역반응신경세포체는 꼬리-조가비핵에서만 관찰되었으며 뒷쪽으로 갈수록 그 수가 줄어들었고 중간부위에서는 NPY면역반응신경세포체가 배쪽내측부분에 많이 분포되어 있는 것을 볼 수 있었다. 5. 줄무늬체에서의 NPY면역반응신경세포체의 모양은 타원형, 난원형, 뭇각형 등 다양하였으나 주로 뭇각형이 많았으며 그 세포돌기도 사방으로 뻗어있었고 그 크기는 15~20 μm로 중간세포가 대부분이었다. Neuropeptide Y (NPY) was first isolated from porcine brain. This discovery has led some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study examined the distribution pattern of NPY-immunoreactive (NPY-IR) neurons in the Striped Field Mouse (Apodemus agrarius coreae) cerebral cortex and striatum, using immunohistochemical method. The results obtained in this study were summarized as followings. 1. NPY-IR neurons distributed in all layer of cerebral cortex. The number of neurons were higher in layer V and VI than in layer I and IV. 2. The shape of neurons was predominantly round or oval in layer I and II, and triangular in layer V and VI. And the processes were parallel to pia mater in layer I and II and were vertical in layer III. 3. The highest number of NPY-IR neurons were found in the perirhinal cortex but a few distinct population were found in the retrosplenial cortex. 4. In stiatum NPY-IR neurons were observed only in caudate-putamen nucleus. 5. The Immunoreactive neurons in caudate-putamen varied in their shape, but most of them were triangular or multiform neurons had omnidirectional processes.
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한국재래산양 태아 및 신생아 척수에서 GFAP 면역반응세포에 관한 형태학적 연구
송치원,정수연,이근좌,이강이,이경열,박일권,박미선,정승혁,조규완,김무강,Song, Chi-Won,Chung, Soo-Youn,Lee, Keun-Jwa,Lee, Kang-Iee,Lee, Kyoung-Youl,Park, Il-Kwon,Park, Mi-Sun,Chung, Seung-Hyuk,Cho, Gyu-Woan,Kim, Moo-Kang 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4
Glial fibrillary acidic protein(GFAP) is one of the intermediate filaments, and used as an astrocyte detection marker. GFAP distribution has been studied on the fetal, neonatal and aged brains. In this study, the GFAP immunoreactive cell localization and distribution in the fetal (30, 45, 60, 90, 105 and 120 days of gestation) and neonate spinal cords of Korean native goat were investigated by immunohistochemistry. Nonpolar radial glial cells initiated to appear at 45 days of gestation. GFAP-immunoreactive processes were extended from central canal to pia matter. Bipolar immumoreactive cells were transformed to monopolar and multipolar immunoreactive cells at 45 days of gestation. Multipolar astrocytes of 60 days of gestation were found within white and gray matters of spinal cord. The number of GFAP-immunoreactive cells were gradually decreased from 90 days of gestation until newborn neonate. The intensity of GFAP immunoreactivity was gradually decreased from 95 days of gestation until newborn neonate. These results suggest that the radial glial cells within the gray and white matters of spinal cord are very fast developed.
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한국분리산 PRV-Ba를 이용한 가토 안구지배신경의 추적 연구
박일권,김무강,신광순,이경열,송치원,이강이,현병화,장규태,정영길,Park, Il-kwon,Kim, Moo-kang,Shin, Kwang-soon,Lee, Kyung-youl,Song, Chi-won,Lee, Kang-iee,Hyun, Byung-hwa,Chang, Kyu-tae,Jeong, Young-gil 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.3
Until now peudorabies virus(PRV) has been used a neurotracer, because of it's properties of retrograde & anterograde transport. But it's anterograde transfort is not perfect, so we tested the applicability of the Bartha strain of PRV(PRV-Ba) isolated from South Korea as a neurotracer in the visual system. We performed immunohistochemical study of the rabbit brain after intravitreal injection of the PRV-Ba. After given survival time(24, 48, 72, 96, 120, 144hrs), the brain was removed and processed immunohistochemical stain for PRV-Ba. The strong PRV immunoreactivity(PRV-ir) were almost oberserved contralaterally in oculomotor neurons, fro example Edinger-Westphal nucleus, trigerminal nucleus of pons and peritrigerminal zone but locus of innervating sensitive neurons. The latter were weak positive and selective. PRV-Ba immunoreactive neurons were stained strongly in nucleus compared to cytoplasm. This study suggests that PRV-Ba isolated from South Korea is also a useful neurotracer in the motor innervated system like other PRV-strain.
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박일권,송치원,이경열,권효정,김무강,이강이,정영길,이남섭,하권수,Park, Il-kwon,Song, Chi-won,Lee, Kyung-youl,Kwon, Hyo-jung,Kim, Moo-kang,Lee, Kang-iee,Jeong, Young-gil,Lee, Nam-seob,Ha, Kwon-soo 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.3
The locus coeruleus(LG) is known to be observed a sexual dimorphism in rat CNS. LC is the largest collections of norepinephrine(NE)-containing neurons in the mammalian brain. Especially in rat, all LC neurons contained NE unlike other mammalians, so that specific reactions were found in the tyrosine-hydroxylase(TH) immunoreactive neurons. Sexual dimorphism of rat LC has affected by genes before sex hormone appeared, thereafter affected by sex hormones. In these day, many scientists founded morphological differences between male and female LC morphology, but differences of entire structure was not founded. Thus we investigated sex differences of the LC neuron's morphology in rat by three-dimensional(3-D) reconstruction using Confocal laser scanning microscopy(CLSM). We reported that neuron's shape was relatively-large multipolar neurons and neuron's processes in dorsal LC proceeded to ventral direction in the male and female rat. Male had a longer anterior-posterior length than female had in dorsal LC. In addition to middle-LC, male rat's LC had a more thicker posterior region but had not viewed in a previous study. In reverse, female rat's LC had a thicker anterior region like a previous study. This results using 3-D reconstruction by CLSM showed that the male's LC was more wide-ranging than female's relatively.
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발생중인 병아리의 사이뇌에서 NPY 양성반응세포의 분포
이미영,박일권,이경열,권효정,송치원,박미선,이강이,하권수,김원식,김무강,Lee, Mee-Young,Park, Il-Kwon,Lee, Kyoung-Youl,Kwon, Hyo-Jung,Song, Chi-Won,Park, Mee-Sun,Lee, Kang-Iee,Ha, Kwon-Soo,Kim, Won-Sik,Kim, Moo-Kang 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.1
This study deals with the distribution and morphological study of the neurons with the neuropeptide Y (NPY) in developing chick brain. The developing brains of Korean native chicks at embryonic days 8 (E8), E10, E12, E14, E16, E18, and E20 were used. The chicks were perfused with 4% paraformaldehyde through the left ventricle and aorta. The brains were removed and transferred into 30% sucrose, and then cut in a cryostat into $60{\mu}m$ in thickness. The sections were immunostained with free-floating and avidin-biotin complex (ABC) methods. The numerous neurons with NPY were first observed in nucleus rotundus of diencephalon at E8. In particular, neurons in nucleus rotundus had the well-developed processes. At E12, the neurons with NPY were distributed widely in diencephalon; nucleus septalis lateralis, medialis, nucleus magnocelluaris preopticus dorsalis, medialis, ventralis, nucleus preopticus medialis, nucleus dorsolateralis anterior thalami pars magnocellularis, and nucleus paraventricularis magnocellularis (paraventricular nucleus) except nucleus rotundus. From E12 to E20, positive neurons were oval-shaped, changed gradually into the spherical- and multipolar-shaped. The shapes of processes were also omnidirectional and the number of those were less than in telencephalon. As the chicks developed, the morphology of neurons with NPY showed the tendency to increase in their sizes and numbers.
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발달중인 Mongolian gerbil의 전뇌에서 TH 면역반응세포의 분포에 관한 연구
이경열,박일권,권효정,박미선,송치원,김무강,장규태,이강이,김원식,박원학,Lee, Kyoung-youl,park, Il-kwon,Kwon, Hyo-jung,Park, Mi-sun,Song, Chi-won,Kim, Moo-kang,Chang, Kyu-tae,Lee, Kang-iee,Kim, Won-sik,Park, Won-hark 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2
The immunocytochemical localization of tyrosine hydroxylase(TH) is examined in the developmental forebrain of mongolian gerbil in order to determine changes in the distribution and cytology of neurons. At each of the different developmental stage, including prenatal(E)14, E16, E18, E20, postnatal(P)0, P2, P4 days, mongolian gerbils were sacrificed. In E14, TH-IR cells were predominantly round or oval in shape and their processes were very short. In olfactory blub, TH-IR cells were begun to appear from E20. In the striatum, we observed only fibers of TH-IR at all ages. From E16, TH-IR perikarya and fiber were present in periventricular nucleus and paraventricular nucleus of hypothalamus. The changes from the early to the late prenatal stages of development appear to be the result of an increase in number of TH-IR perikarya and processes. These results were shown slight differences to other rodents.
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한우(韓牛) 안구(眼球)의 망막신경절세포(網膜神經節細胞) 수(數)와 분포(分布)에 관(關)한 연구(硏究)
김무강,조성환,류시윤,김교준,김상근,신태균,이강이,Kim, Moo-kang,Cho, Sung-whan,Ryu, Si-yun,Kim, Kyo-joon,Kim, Song-keun,Shin, Ta-kyun,Lee, Gang-iee 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1
The number and distribution of the retinal ganglion cells in the 2 years old Korean native cattle was determined from whole fiat mounted preparation stained with methylene blue and thionin. The results were summarized as follows. 1. The total number of retinal ganglion cells was estimated to be 3,085,200 in the bovine retina ranging from $2,214mm^2$ in total area. 2. Visual streak was recognized at the area 2.5mm superior to the optic disc and ganglion cell density drops off rapidly to the directions superior to and inferior to the visual streak. 3. Area centralis ($6,800cells/mm^2$) was located at the area 10mm temporally from the point of 3mm superior to the optic disc. 4. The number of ${\alpha}-type$ ganglion cells (above $15{\mu}$) was 57,000 in the bovine retina and ${\alpha}-type$ ganglion cells constituted 18.5% of the total cells. 5. The relative frequency of ${\alpha}-type$ ganglion cells was higher in the peripheral regions than in the visual streak, especially higher in the superior-temporal quadrant than in other region of the bovine retina.
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