랫드에서 백두옹과 청호의 경구투여에 의한 반복 투여독성 시험과 면역 활성 평가

안인정,정지윤,권중기,이진석,이성호,박영석,박병권,김상기,김병수,조성대,최창순,이병희,강병곤 한국식품위생안전성학회 2012 한국식품위생안전성학회지 Vol.27 No.1

This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of Pulsatilla koreana and Artemisiae annuae in Sprague-Dawley rats. The female rats were treated with Pulsatilla koreana and Artemisiae annuae of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of Pulsatilla koreana and Artemisiae annuae such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of Pulsatilla koreana. The increase immune cells in the high dose group of Pulsatilla koreana might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, Pulsatilla koreana and Artemisiae annuae is physiologically safe and improve immunomodulating activity.

Fucoidan의 면역세포 활성 및 위암 세포주에서의 항암효과

안인정,정지윤,조성대,권중기,김혜리,유현주 한국식품위생안전성학회 2012 한국식품위생안전성학회지 Vol.27 No.4

This study was designed to investigate the effect of fucoidan on the activation of macrophage and on induction of apoptosis in AGS cell. To measure the activity of macrophages, NO and TNF-α assays were performed in Raw 264.7 cell. Treatment with fucoidan significantly increased production of NO and TNF-α, indicating activation of macrophages. The result of MTT assay shows that cell viability was significantly decreased in a dose and time-dependent manner. Fucoidan increased to enhance mitochondrial membrane permeability, as well as the cytochrome c release from the mitochondria. Fucoidan decreased Bcl-2 and XIAP expression, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9were increased, and the inactivation of Akt was decreased in a time-dependent manner. Caspase inhibitor, z-VADFMK,canceled the apoptosis of fucoidan, expression of Bax and caspase-9 were decrease. These results indicate that fucoidan induces activation of macrophage and apoptosis through activation of caspase on AGS cell.

Bacterial communities in the feces of insectivorous bats in South Korea

안인정,김벼리,주성배,김기현,이택우 한국생태학회 2024 Journal of Ecology and Environment Vol.48 No.2

Bats serve as vectors and natural reservoir hosts for various infectious viruses, bacteria, and fungi. These pathogens have also been detected in bat feces and can cause severe illnesses in hosts, other animals, and humans. Because pathogens can easily spread into the environment through bat feces, determining the bacterial communities in bat gua- no is crucial to mitigate potential disease transmission and outbreaks. This study primarily aimed to examine bacterial communities in the feces of insectivorous bats living in South Korea. Fecal samples were collected after capturing 84 individuals of four different bat spe- cies in two regions of South Korea, and the bacterial microbiota was assessed through next generation sequencing of the 16S rRNA gene. The results revealed that, with respect to the relative abundance at the phylum level, Myotis bombinus was dominated by Firmic- utes (47.24%) and Proteobacteria (42.66%) whereas Miniopterus fuliginosus (82.78%), Rhi- nolophus ferrumequinum (63.46%), and Myotis macrodactylus (78.04%) were dominated by Proteobacteria. Alpha diversity analysis showed no difference in abundance between species and a significant difference (p < 0.05) between M. bombinus and M. fuliginosus. Beta-diversity analysis revealed that Clostridium, Asaia, and Enterobacteriaceae_g were clustered as major factors at the genus level using principal component analysis. Addi- tionally, linear discriminant analysis effect size was conducted based on relative expres- sion information to select bacterial markers for each bat species. Clostridium was relatively abundant in M. bombinus, whereas Mycoplasma_g10 was relatively abundant in R. fer- rumequinum. Our results provide an overview of bat guano microbiota diversity and the significance of pathogenic taxa for humans and the environment, highlighting a better understanding of preventing emerging diseases. We anticipate that this research will yield bioinformatic data to advance our knowledge of overall microbial genetic diversity and clustering characteristics in insectivorous bat feces in South Korea.

국화과 추출물의 암세포 증식 억제 효과

안인정(In-Jung An),권중기(Jung-Ki Kwon),이진석(Jin-Seok Lee),박하승(Ha-Seung Park),김동찬(Dong-Chan Kim),최병준(Byung-Jun Choi),이규민(Kyu-Min Lee),박용진(Youg-Jin Park),정지윤(Ji-Youn Jung) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.5

국화과 꽃은 우리나라에서 전통적으로 항염증과 항산화 치료에 사용되었다. 본 연구에서는 국화과 추출물이 인간 위암세포 AGS, 인간 유방암세포 MDA-MB-231과 SKBR-3 암세포에서 성장을 억제하고 세포자멸사를 유발하는지 확인하였다. AGS, MDA-MB-231 그리고 SK-BR-3 암세포의 성장을 MTT로 측정하였다. 14종의 국화과 추출물을 24시간 동안 50, 100, 200 μg/mL의 농도로 처치하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부, 낙동구절초 전초, 산국 전초 그리고 해국 꽃 추출물에서 암세포의 성장을 농도 의존적으로 억제시켰다. 우리나라 여성에서 가장 많이 발생하는 유방암세포인 MDA-MB-231 암세포에서 세포자멸사를 확인하기 위해 DAPI 염색을 수행하였다. MTT assay에서 암세포를 억제시킨 6종의 국화과 추출물중 3종인 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 처치한 세포에서 핵의 응축이 농도 의존적으로 존재함을 형광현미경으로 확인하였다(×200). 세포자멸사에 관련된 단백질의 발현을 알아보기 위해서 western blot으로 확인하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 25, 50 ㎍/mL의 농도로 24시간 동안 MDAMB-231 암세포 처치 후 cell lysate를 얻어 Bcl-2, Bax 그리고 p53의 변화를 관찰하였다. 한라구절초 전초, 포천구절초전초 그리고 삼잎국화 지하부에서 anti-apoptotic 분자인 Bcl-2 단백질은 감소하고 반대로 pro-apoptotic 분자인 Bax와 p53 단백질은 증가하였다. 결과적으로 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부 추출물은 유방암 세포의 성장을 억제하고 apoptosis를 유발시키므로 암예방제나 치료제로 개발될 수 있을 것으로 사료된다. Dried Compositae flowers have traditionally been used for the treatment of anti-inflammatory and anti-oxidative stress in Korea. This paper investigates the effects of Compositae extracts on the inhibition of proliferation and apoptosis of human gastric cancer AGS cells, human breast cancer MDA-MB-231 cells, and SK-BR-3 cells. The proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells were determined by MTT assay. Several Compositae extracts inhibited proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells in a dose-dependent manner. To assess the apoptosis of Compositae extracts, the nuclei of MDA-MB-231 cells were stained with DAPI. The presence of chromatin condensation in the Compositae extract-treated cells was detected on a fluorescent microscope (×200). We conducted Western blot analysis of changes in Bcl-2, Bax, and p53 protein expression levels. Apoptosis by Chrysanthemum zawadskii subsp. coreanum, Chrysanthemum zawadskii var. tenuisectum and Rudbeckia laciniata var. hortensis treatment created a decrease in Bcl-2 expression, whereas the expression of Bax and p53 were increased. These results indicate that Chrysanthemum zawadskii subsp. coreanum, Chrysanthemum zawadskii var. tenuisectum and Rudbeckia laciniata var. hortensis inhibit breast cancer cell growth through the induction of apoptosis.

Dietary composition of two coexisting bat species, Myotis ikonnikovi and Plecotus ognevi, in the Mt. Jumbong forests, South Korea

주성배,안인정,김선숙 한국생태학회 2023 Journal of Ecology and Environment Vol.47 No.4

Background: Many insectivorous bats have flexible diets, and the difference in prey item consumption among species is one of the key mechanisms that allows for the avoidance of interspecies competition and promotes coexistence within a microhabitat. In Korea, of the 24 bat species that are known to be distributed, eight insectivorous bats use forest areas as both roosting and foraging sites. Here, we aimed to understand the resource partitioning and coexistence strategies between two bat species, Myotis ikonnikovi and Plecotus ognevi, cohabiting the Mt. Jumbong forests, by comparing the differences in dietary consumption based on habitat utilization. Results: Upon examining their dietary composition using the DNA meta-barcoding approach, we identified 403 prey items (amplicon sequence variants). A greater prey diversity including Lepidoptera, Diptera, Coleoptera, and Ephemeroptera, was detected from M. ikonnikovi, whereas most prey items identified from P. ognevi belonged to Lepidoptera. The diversity index of prey items was higher for M. ikonnikovi (H’: 5.67, D: 0.995) than that for P. ognevi (H’: 4.31, D: 0.985). Pianka’s index value was 0.207, indicating little overlap in the dietary composition of these bat species. Our results suggest that M. ikonnikovi has a wider diet composition than P. ognevi. Conclusions: Based on the dietary analysis results, our results suggests the possibility of differences in foraging site preferences or microhabitat utilization between two bat species cohabiting the Mt. Jumbong. In addition, these differences may represent one of the important mechanism in reducing interspecific competition and enabling coexistence between the two bat species. We expected that our results will be valuable for understanding resource partitioning and the coexistence of bats inhabiting the Korean forests.

Resveratrol Induces Apoptosis through PI3K/Akt and p53 Signal Pathway in MDA-MB-231 Breast Cancer Cells

권중기,안인정,김혜리,이성호,박영석,박병권,김병수,김상기,정지윤 한국실험동물학회 2012 한국실험동물학회 학술발표대회 논문집 Vol.2012 No.8

국화로부터 추출한 천연염료에 대한 급성경구독성 및 피부자극성 시험에 대한 연구

권중기,정지윤,안인정,이진석,김혜리,박하승,김동찬,최병준,이규민,박용진 한국식품위생안전성학회 2012 한국식품위생안전성학회지 Vol.27 No.2

This study was designed to evaluate to acute oral toxicity and skin irritation of Chrysanthemum dye in Sprague-Dawley (SD) rats. SD rats were orally treated with Chrysanthemum dye at a dose of 0, 1 and 2 ml/kg body weight. After oral administration, the rats were observed for 14days. In primary skin irritation test, SD rats were dermally treated with Chrysanthemum dye and observed for 3 days. To ensure the safety of Chrysanthemum dye such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the histopathological test, the mortality and skin irritation. As a result, no significant differences were found in body weight, feed intake and histopathological test between control and Chrysanthemum dye treated group. In the result of skin irritation test, Chrysanthemum dye did not induce erythema and edema after topical application. Primary irritation index was “0” in the test. Therefore, it is suggested that Chrysanthemum dye has no effect on acute toxicity and side effect in SD rats and is non-irritant material based on the score “0” of primary irritation index.

제독유황에 대한 3개월간 랫드에서 반복경구투여 독성에 관한 연구

이진석,권중기,한상현,안인정,김소정,이성호,박영석,박병권,김병수,김상기,김일호,최창순,정지윤 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.3

This study was demonstrate a repeated oral dose toxicity of detoxication sulphur in 8-weeks-old female Sprague-Dawley (SD) rats. The rats were treated with dose of 0.2%, 1%, 5% detoxication sulphur and 1% sulphur of feed consumption administered for 13 weeks. To evaluate the safety of detoxication sulfur, we examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. As a result, no significant differences in body weight, feed intake, hematological examination and histopathological between control and detoxication sulphur treatment group were found. Serum biochemical results were not shown significant differences in 0.2% and 1% the treated groups compared with control group. But glucose level were decrease, also ALT and ALP level were increase in 5% treated group. All of these results indicate that 1% detoxication sulphur of feed consumption may be safety in SD rat.

국내 일 병원 중환자실에서 관찰한 섬망 환자의 예후

손정현,박진영,라세희,신증수,손인정,오주영,안지선,안석균,김재진 대한신경정신의학회 2014 신경정신의학 Vol.53 No.6

Objectives The purpose of this study is to evaluate the impact of delirium on clinical outcomes in intensive care unit (ICU) patients in a Korean general hospital. Methods All patients admitted to ICU from March 1, 2013 to October 31, 2013 were assessed by Confusion Assessment Method adapted for use in the ICU for delirium daily, and consistently comatose patients were excluded for analysis. Differences in clinical outcomes (mortality, length of hospital stay, length of ICU stay) were analyzed between delirious patients and non-delirious patients. Subsequently, the impact of delirium on clinical outcomes was analyzed with adjusting for covariates including surgery, age, emergent admission, presence of surgery, and severity of illness. Results The analysis included 129 delirious patients and 469 non-delirious patients. As primary outcome, mortality, length of stay (hospital day, ICU-stay) were significantly high in the delirious group. The association remained the same after adjusting for the covariates. Conclusion The results correspond with those of previous research studies conducted in foreign ICU. Based on this observation that delirium also has an impact on clinical outcomes in Korean ICU, integrative and in-depth investigation on ICU delirium will be needed. 중환자실 재실 중 섬망을 경험하는 환자는 다른 인자들의영향을 통제한 후에도 퇴원시 사망률이 높고, 재실 기간 및입원 기간이 더 길어 본 연구의 가설과 일치하는 결과를 보였다. 이 결과는 국외 연구들에서 보고되어 온 바와 일치하며, 국내 종합병원 중환자실 환경의 환자군을 대상으로 하여그 상관관계를 처음으로 확인하였다는 의의가 있다. 이러한결과는 향후 국내 의료 환경에서 발생하는 섬망에 대한 탐색과 이에 대한 적절한 중재를 연구하는 데 기초 자료로서 활용될 것으로 기대한다.

Effects of β-glucan on activity of macrophage function and anticancer effect in breast cancer cells

이진석,김소정,권중기,안인정,이성호,박영석,박병권,김병수,김상기,정지윤 한국실험동물학회 2011 한국실험동물학회 학술발표대회 논문집 Vol.2011 No.2