감마선 조사와 푸코이단/라미나린 병용처리가 돈육 패티의 품질에 미치는 영향

김현주,최종일,박진규,송범석,김재훈,윤요한,김천제,신미혜,변명우,이주운,Kim, Hyun-Joo,Choi, Jong-Il,Park, Jin-Gyu,Song, Beom-Seok,Kim, Jae-Hun,Yoon, Yo-Han,Kim, Cheon-Jei,Shin, Mee-Hye,Byun, Myung-Woo,Lee, Ju-Woon 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.1

The objective of this study was investigated to apply the fucoidan and laminarin extract from low-grade Undaria pinnatifida to real food product, a pork patty. Pork patties added with fucoidan and laminarin showed lower lipid oxidation and the inhibition against lipid oxidation was shown to be dependent on the irradiation doses. The Hunter color values of pork patty added with fucoidan increased significantly with an increase of irradiation dose. The hardness profiles of the patties with fucoidan and laminarin was decreased, but the amount of water in the patties was increased. Also, the combination of gamma irradiation and addition of fucoidan and laminarin was shown to be effective for the microbiological control. These results suggested that gamma irradiation and fucoidan and laminarin treatment showed the positive effect on microbial stability and quality of the pork patty.

산지하천에서 빛가 초식에 의한 부착조류의 생체량변화

김현주,윤해순,김진수,김현우,주기재 ( Hyun Joo Kim,Hae Soon Yoon,Jin Soo Kim,Hyun Woo Kim,Gea Jae Joo ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.4

Effects of light and grazing on the biomass development of periphyton were evaluated using natural and artificial substrata (unglazed tile: 3.7×9.5x2cm) in a partially shaded 2nd order mountain stream. In open canopy (relative light input 20∼50%), a relatively high biomass (chl. a: 9.5±3.0 mg/m^2, n=25) was maintained than that of closed canopy(<6% of light input. 3.2±0.9 mg/m^2, n= 17). Due to shading and frequent rainfalls, biomass was lower during the summer than in the spring and winter. During the spring, colonization of periphyton on artificial substrata occurred faster than in winter and it took a minimum of 5∼6 weeks to reach the biomass of natural substrata (8∼9mg/m^2). When snails were allowed to freely enter the chamber (mean density: 114 ind./m^2) with artificial substrata, biomass accumulation (chl. a, AFDW) was low compared to the snail excluded enclosure (50∼90% of ungrazed substrata). It was concluded that the light input on periphyton biomass In a partially shaded stream played a more important role than glazing.

도시철도 역사 유니버설디자인 적용 및 효과 분석: 수서역을 대상으로

김현주,김진희,김장욱,김진경,Kim, Hyeon-Ju,Kim, Jin-Hui,Kim, Jang-Uk,Kim, Jin-Gyeong 대한교통학회 2016 교통 기술과 정책 Vol.13 No.6

평택시 군용비행장 주변지역 주민건강조사

김현주,노상철,권호장,백기청,이무용,정재윤,임명호,구미진,김창훈,김혜영,임정훈,김동현,Kim, Hyun-Joo,Roh, Sang-Chul,Kwon, Ho-Jang,Paik, Ki-Chung,Rhee, Moo-Yong,Jeong, Jae-Yun,Lim, Myung-Ho,Koo, Mi-Jin,Kim, Chang-Hoon,Kim, Hae-Young,Lim, Je 대한예방의학회 2008 예방의학회지 Vol.41 No.5

Objectives : We conducted an epidemiologic survey to evaluate the effect of the aircraft noise exposure on the health of the residents near the military airbases in Pyeongtaek City. Methods : The evaluation of environmental noise level, questionnaire survey, and health examination were performed for 917 residents. The study population consisted of four groups: subjects who lived in the village close to the fighter airbase (high exposure), subjects who lived along the course of fighters (intermediate exposure), and subjects near a helicopter airbase, and the control group. Results : The prevalence of the aircraft noise related accident and irritable bowel syndrome in the exposure groups were higher than that of the control group. The risks of noise induced hearing loss, hypertension and diabetes mellitus were higher in the exposed groups than in the control group. The prevalence of anxiety disorder and primary insomnia were higher in the exposed groups than in the control group. Prevalence odd ratios of the risk for primary insomnia after adjusting age, sex, agricultural noise, and occupation were 4.03 [95% confidence interval (95% Cl) 1.56-10.47] for the subject near the helicopter airbase, 1.23 (95% Cl 0.40-3.76) for those intermediately exposed to fighter noise, and 4.99 (95% Cl 2.14-11.64) for those highly exposed to fighter noise. Conclusions : The results of the present study suggest that the aircraft noise may have adverse effects on hearing function, cardiovascular health and mental health. Therefore, it seems to be needed to take proper measures including the control of the aircraft noise and the management of the exposed people's health.

CIS 고려인 디아스포라 소설 연구

장사선(Jang Sa Seon),김현주(Kim Hyeon Ju) 한국현대소설학회 2004 현대소설연구 Vol.- No.21

This study aims to examine the general trend of novels written by Soviet-Korean in C I S. Soviet-Koreans in this Central Asian area have moved from the Far Eastern area of Russia by the enforcement of the Stalinian policy of deportation in 1937. The authors have written a number of novels describing the life of diaspora experienced by Korean residents. To be more specific, the main focus of these novels is centered onto the issues such as the adaptation to the socialist system, the discord or accord with the different nation, the self-identity, and so on. The representative authors and their works are as follow: Seil Kim`s HongBeomDo and Jun Kim`s A happening of one hundred and fifty thousand won that depict the heros of anti-Japanese fight, Gicheol Kim`s When red stars were seen and a group works, The melody of Syrdarya that praise the socialism, sunflower and ``자말러, you are my life that treat the agony of identity as Koreans in a foreign country, and several works of Anatoli Kim and Lee-Jin that reflect the times of the various open door, On the whole, each novel expresses successfully the scenes of life with a different ideology and in the unfamiliar environment and embodies such troubles adequately into the form of literature. However, there also exist some problems of the plain development of plot. the dichotomic way of thinking, the excessive exposure of the theme, and so forth. After the collapse of the Soviet Union around 1990. a rapid conversion of direction toward modernism has occurred. With the opening of the exchange gate between Korea and CIS, there have been many changes in the content and publication of the works. More extensive research is needed.

작은뾰족민달팽이(Deroceras reticulatum)의 발육과 수명에 미치는 온도효과

김현주,배순도,이건휘,윤영남,박성태,최병렬,박정규,Kim, Hyun-Ju,Bae, Soon-Do,Lee, Geon-Hwi,Yoon, Young-Nam,Park, Sung-Tae,Choi, Byeong-Ryeol,Park, Chung-Gyoo 한국응용곤충학회 2009 한국응용곤충학회지 Vol.48 No.4

$24^{\circ}C$에서 작은뾰족민달팽이의 알 크기 (단경$\times$장경)는 $1.91{\times}2.19$ mm, 무게는 3.3 mg 이었다. 산란 직후의 알에서부터 발육하여 우화한 성체가 최초 산란 시까지 17주간의 무게는 발육일수가 경과할수록 증가하였으며, 온도별 무게는 $20^{\circ}C$에서 가장 무거웠으며, 다음은 24, 16 및 $28^{\circ}C$ 순이었다. 작은뾰족민달팽이가 산란한 난괴수의 부화율은 $20^{\circ}C$에서 95%로 가장 높았으며, 다음은 24, 16 및 $28^{\circ}C$의 순으로 유의한 차이가 있었다. 난괴당 알수는 $20^{\circ}C$에서 20개로 가장 많았으며 $28^{\circ}C$에서 10개로 가장 적었다. 누적 산란 일수는 $20^{\circ}C$에서 15일로 가장 길었으며, $24^{\circ}C$에서 9일로 가장 짧았고, 산란기간은 84~134일로 $16^{\circ}C$에서 가장 짧았으며, $20^{\circ}C$에서 가장 길었다. 그리하여 총 산란수는 $20^{\circ}C$에서 217개로 가장 많았고, $16^{\circ}C$에서 105개로 가장 적었다. 작은뾰족민달팽이의 알기간은 12~19일, 유체기간은 51~68일, 성체수명은 85~134일 이었으며, 알에서부터 성체수명까지의 총 생존기간은 $20^{\circ}C$에서 216일로 가장 길었으며, 다음은 $24^{\circ}C$에서 193일, $16^{\circ}C$에서 170일 및 $28^{\circ}C$에서 151일 순 이었다. 따라서 작은뾰족민달팽이의 산란, 발육 및 성체수명에 가장 유리한 온도는 $20^{\circ}C$로 여겨졌다. The egg size and weight of the gray field slug, Deroceras reticulatum Muller, was $1.91{\times}2.19$ mm and 3.3 mg at $24^{\circ}C$, respectively. Its weight increased from newly laid eggs to adults for 17 weeks at four temperature regimes and was heaviest at $20^{\circ}C$, followed by 24, 16 and $28^{\circ}C$. Likewise, hatching rate of the egg batches was highest with 95% at $20^{\circ}C$, followed by 24, 16 and $28^{\circ}C$ with significant differences. The number of eggs in the batches was increased with 20 at $20^{\circ}C$ while it was only 10 at $28^{\circ}C$. The average accumulated egg-laying days was longest with 15 days at $20^{\circ}C$ while shortest with 9 days at $24^{\circ}C$. The range of egg-laying period was shortest with 84-134 days at $16^{\circ}C$ and longest at $20^{\circ}C$. Thus, the total number of eggs laid by adult D. reticulatum was highest with 217 at $20^{\circ}C$ and lowest with 105 at $16^{\circ}C$. The egg period was 12-19 days while the juvenile period and adult longevity were 51-68 days and 85-134 days, respectively. The life span of D. reticulatum from egg to adult was longest with 216 days at $20^{\circ}C$, followed by 193 days at $24^{\circ}C$, 170 days at $16^{\circ}C$ and 151 days at $28^{\circ}C$. Accordingly, the most favorable temperature for the oviposition, development and longevity of D. reticulatum is $20^{\circ}C$.

Semliki Forest Virus 감염은 뉴우런의 탈수초를 유발한다

김현주,사영희,홍성갑,Kim, Hyun Joo,Sa, Young-Hee,Hong, Seong-Karp 한국정보통신학회 2017 한국정보통신학회논문지 Vol.21 No.6

척수신경절의 신경 세포와 슈반세포의 공동 배양으로 수초화 형성 세포 집단이 제조되었다. 슈반세포와 뉴런 세포가 쥐의 배아의 척수신경절로 부터 각각 in vitro에서 분리되었다. 배양된 슈반세포와 뉴런 세포는 동일한 평판접시에서 공동배양 되었다. 본 실험과정은 다음과 같은 4 단계로 구성되어 있다 : 첫 번째 단계는 배아의 척수 신경절 세포의 현탁 과정, 두 번째 단계는 안티 mitotic cocktail의 추가 과정, 세 번째 단계는 척수신경절 세포의 정제 과정, 및 네 번째 단계는 척추 신경절 세포에 슈반 세포의 추가 과정이다. 이들 세포들은 때 수초화가 진행되었다. 이렇게 수초화된 공동 배양은 Semliki forest virus에 의해 감염되었고 그 때 탈수초화 과정을 유발시켰다. 우리는 수초화된 뉴런에 존재하는 peripheral myelin protein 22의 항체를 이용하여 수초화 과정과 탈수초화 과정을 확인하였다. We constructed a population of myelinated cells with co-culture of neuronal cells and Schwann cells from DRG. Schwann cells and neuronal cells were isolated from dorsal root ganglion (DRG) in embryos of rat in vitro respectively. The cultured Schwann cells and cultured neuronal cells, respectively were co-cultured in a same plate. This procedure contains following four steps: first step of suspension of the embryonic dorsal root ganglion cells, second step of addition of anti-mitoticcocktail, third step of purification of dorsal root cells, and fourth step of addition of Schwann cells to dorsal root ganglion cells. These cells were performed accomplishment of myelination. This myelinated co-culture system was infected by Semliki forest virus and then induced demyelination processing in this myelinated co-culture. We identified myelination and demyelination processing using antibody of peripheral myelin protein 22 (PMP 22) meaning presence of myelinated neuron.

Association of gastric cancer with cytochrome P450 2C19 single-nucleotide polymorphisms in Koreans

김현주,Hye-Jung Park,,Sang-Gyu Lee,,Hye-Suk Lee,,Won-Cheol Park,,Jeong-Joong Kim,,Gyung-Jae Oh,Yun-Kyung Kim 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.4

Cytochrome P450 2C19 (CYP2C19) is a clinically important enzyme involved in the metabolism of therapeutic drugs, including (S)-mephenytoin, omeprazole, proguanil, and diazepam. Individuals are characterized as either extensive metabolizers (EM) or poor metabolizers (PM) on the basis of CYP2C19 enzyme activity. The PM phenotype occurs in 2 - 5% of Caucasians, but in 18 - 23% of Asians. To clarify the association between CYP2C19 polymorphisms and gastric cancer in Koreans, we investigated CYP2C19 genotypes (CYP2C19*1, *2, and *3) in 109 patients with gastric cancer and 211 controls. Normal (CYP2C19*1) and defective alleles were detected with polymerase chain reaction/restriction enzyme analysis. CYP2C19 has three hereditary genotypes: homozygous EM, with high enzymatic activity; heterozygous EM, with moderate enzymatic activity; and PM, with no enzyme activity. We found that CYP2C19 heterozygous EM is more closely associated with gastric cancer than is homozygous EM. Because the CYP2C19 genotype varies in Koreans, a genotyping test is desirable to prevent gastropathy recurrence in patients before their doses of omeprazole are reduced during maintenance therapy.

스마트폰 동영상을 활용한 자가관찰 방법의 자율실습이 간호학생의 핵심기본간호술 교육에 미치는 효과

김현주,Kim, Hyun-Ju 한국정보통신학회 2018 한국정보통신학회논문지 Vol.22 No.9

핵심기본간호술의 교육효과를 높이기 위한 방법으로 스마트폰 동영상을 활용한 자가관찰 방법의 자율실습이 간호학생의 자기주도적 학습능력, 핵심간호술기 수행능력, 수행자신감 및 실습만족도에 미치는 효과를 규명하기 위해 비동성 대조군 사후설계의 유사실험연구를 수행하였다. 대상자는 P대학교 간호학과 4학년을 대상으로 하였으며, 실험군 54명, 대조군 51명이었다. 연구결과 자기주도적 학습능력은 두 군간에 유의한 차이가 없었으나 실험군의 사전- 사후점수 차이에는 유의하게 향상되었다(p=<.001). 수혈간호, 수술전간호의 핵심간호술기 능력과 수행자신감, 실습 만족도는 두 군간의 유의한 차이가 없었다. 결론적으로 스마트폰 동영상촬영을 활용한 자가평가 학습법은 자기주도적 학습능력을 향상시켜 술기의 정확성과 숙련성을 갖출 수 있는 동기부여에 효과적인 방법이라 사료된다. This study was done to identify the effects of self-monitoring method using smartphone video on self-directed learning ability, competency in nursing skills self-confidence and practice satisfaction of nursing students. It was designed a quasi-experimental study with a pre-post test. The participants were 54 students in the experimental group and 51 students in the control group. There was no significant difference in self-directed learning ability (p = .450) between the groups, but there was a significant difference between the pre-post scores of the experimental group. There was no significants difference of competency in nursing skills, self-confidence and practice satisfaction between the two groups. In conclusion, the self-assessment learning method using smartphone video during the self-practice is considered to be an effective method to improve the self-directed learning ability and to ensure the accuracy and skill of the technique.

The Role of Receptor Activator of NF-κ Ligand in Smooth Muscle Cell Proliferation

김현주,Kim, Hyun-Ju Korean Society of Life Science 2006 생명과학회지 Vol.16 No.6

Smooth muscle cell (SMC)의 증식은 혈관성장에 의한 질환의 발병기전의 중요한 요소이다. 혈관 손상 후 SMC의 성장조절에 대한 분자적 기작에 대한 연구는 치료제 개발에 있어 중요한 의미를 지닌다. 이에, 본 연구에서는 TNF family인 RANKL가 SMC의 증식을 촉진함을 입증하였다. RANKL는 p21의 발현을 감소시키고 p21의 promoter활성을 저해함으로써 SMC의 성장을 증가시켰다. 또한 ERK와 p38 MAPK의 활성이 RANKL에 의해 증가하였으며, ERK/p38의 저해제는 RANKL에 의해 유도되는 SMC의 성장을 완전히 억제하였다. 이러한 결과는 ERK와 p38 MAPK가 RANKL에 의해 유도되는 SMC의 증식에 중요한 역할을 함을 보여주는 것이다. 즉, RANK-RANKL-ERK/p38이 SMC의 증식을 매개하는 중요 분자이며, 이들 분자는 혈관 질환을 막는 새로운 치료제 개발의 표적분자가 될 수 있음이 입증되었다. Smooth muscle cell (SMC) proliferation is important in the pathogenesis of vascular proliferative disorders. Understanding of the molecular mechanism underlying SMC growth after arterial injury would have therapeutic implications. Here we report that receptor activator of $NF-{\kappa}B$ ligand (RANKL), a member of tumor necrosis factor (TNF) family, promotes the proliferation of SMC, leading to decreased expression of p21 and enhancement of SMC growth. ERK and p38 phosphorylation was enhanced after RANKL treatment in SMC. Inhibition of ERK/p38 MAPK activity by PD98059/SB203580 completely abolished RANKL-induced proliferation of SMC, indicating ERK and p38 MAPK are essential for RANKL-induced SMC proliferation. Taken together, our findings demonstrate that RANK-RANKL-ERK/p38 pathway is important for proliferation of SMC and that these molecules may be the new therapeutic targets for the prevention of vascular diseases.