백서 치주인대세포의 RANKL 발현에 대한 p38 MAPK의 역할

김종철,김영준,정현주,김옥수,Kim, Chong-Cheol,Kim, Young-Joon,Chung, Hyun-Ju,Kim, Ok-Su 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.3

Recent studies have demonstrated that human periodontal ligament cells express receptor activation of nuclear factor ${\kappa}B$ ligand (RANKL) which enhances the bone resorbing activity of osteoclasts differentiated from hematopoietic preosteoclasts. The purpose of this study is to determine the effects of p38 MAPK and JNK kinase upon regulating RANKL and OPG in response to $IL-1{\beta}$(l ng/ml) in rat periodontal ligament cells. Soluble RANKL was measured by immunoassay. The effects of p38 MAPK on RANKL and OPG expression was determined by RT-PCR. The results were as follows: 1. Periodontal ligament cells which stimulated by $IL-1{\beta}$ increased soluble RANKL synthesis by dose-dependent pattern. 2. p38 MAP kinase inhibitor (SB203580) showed regulation of soluble RANKL expression by dose-dependent manners. 3. p38 MAP kinase inhibitor (SB203580) regulated the expression of RANKL, but it dose regulate the expresseion of OPG. 4. JNK (c-jun $NH_2-terminal$ kinase) inhibitor (PD98059) did not regulate mRANKL and mOPG. These results suggested that p38 MAPK play a significant role in RANKL gene expression.

식물 및 미생물 유래 유기농자재 살충효과: 단감해충 감꼭지나방, 톱다리개미허리노린재

김종철,유정선,송민호,이미롱,김시현,이세진,김재수,Kim, Jong Cheol,Yu, Jeong Seon,Song, Min Ho,Lee, Mi Rong,Kim, Sihyeon,Lee, Se Jin,Kim, Jae Su 한국응용곤충학회 2016 한국응용곤충학회지 Vol.55 No.4

단감 해충의 효과적인 방제를 위하여, 현재까지 주로 화학 살충제가 사용되어 왔으나, 최근 화학 살충제의 과다 사용은 해충 저항성과 농약 잔류 문제라는 심각한 문제를 야기하고 있다. 본 연구에서는 친환경 유기농자재 (Eco-Friendly Organic Pesticides; EFOP)의 주요 단감 해충인 감꼭지나방과 톱다리개미허리노린재에 대한 살충 활성을 검토하였다. 현재 유통되고 있는 친환경 유기농자재 11 종을 실험실 조건에서 대상 해충에 분무 처리하였다. 대조 약제는 화학 살충제인 buprofezin+dinotefuran (20+15) 수화제를 사용하였다. 감꼭지나방에 대한 유기농자재의 살충 효과를 평가하는 과정에서, 감꼭지나방 유충의 개체수 부족 문제로 인하여 배추좀나방 유충을 1차 실험충으로 사용하였으며, 세 종류의 친환경 유기농자재를 사전 선발하였다. 선발된 친환경 유기농자재는 목초액 (EFOP-1), 회화나무, 양명아주, 멀구슬나무의 혼합추출물 (EFOP-2), Bacillus thuringiensis subsp. aizawai NT0423 (EFOP-11)이었으며, 선발된 유기농자재의 감꼭지나방 유충에 대한 살충력 실험 결과, EFOP-2의 처리구에서 생충율이 27.7% (5 일차), 13.3% (7 일차), 6.7% (10 일차)로 가장 높은 살충 효과를 보였다. 톱다리개미허리노린재에 대한 살충 효과 평가에서는 회화나무, 양명아주, 멀구슬나무의 혼합추출물 (EFOP-2, EFOP-9)과 고삼, 데리스의 혼합추출물 (EFOP-10)이 높은 살충 효과를 보였다. 특히 EFOP-2의 처리구에서 생충율이 20.0% (5 일차), 16.7% (10 일차)로 가장 높은 살충 효과를 보였다. 결론적으로, 친환경 유기농자재인 EFOP-2를 이용하여 단감의 주요 해충인 감꼭지나방과 톱다리개미허리노린재를 포함한 다양한 노린재 해충에 대한 방제가 가능할 것으로 판단된다. Chemical pesticides have been used to control persimmon pests, however the overuse of the pesticides caused insect resistance, followed by failure in pest management and residual problems. Herein we investigate the potential of eco-friendly organic pesticides (EFOP) on the control persimmon pests, Stathmopoda masinissa (persimmon fruit moth) and Riptortus pedestris (bean bug). Ten commercially available plant-derived organic pesticides and one microbial pesticide were sprayed on the target insects in laboratory conditions. The chemical pesticide, buprofezin+dinotefuran wettable powder served as a positive control. In the first bioassay against persimmon fruit moth, alternatively Plutella xylostella larvae were used due to the lack of persimmon fruit moth population from fields, and three organic pesticides showed high control efficacy, such as pyroligneous liquor (EFOP-1), the mixture of Chinese scholar tree extract, goosefoot and subtripinnata extracts (EFOP-2) and Bacillus thuringiensis subsp. aizawai NT0423 (EFOP-11). When the three selected organic pesticides were treated on the persimmon fruit moths, the EFOP-2 treatment showed the highest control efficacy: 27.7% (5 days), 13.3% (7 days) and 6.7% (10 days) of survival rates. In the bioassay against bean bugs, the mixture of Chinese scholar tree, goosefoot and subtripinnata extracts (EFOP-2 and EFOP-9) and the extracts of sophora and derris (EFOP-10) showed high control efficacy, particularly the highest in the treatment of EFOP-2: 20.0% (5 days) and 16.7% (10 days) of survival rates. These results suggest that the mixture of Chinese scholar tree, goosefoot and subtripinnata extracts (EFOP-2) has high and multiple potential in the management of the persimmon pests.

Effects of Acanthopanax senticosus herb-acupuncture on cell proliferation and nitric oxide synthase expression in dentate gyrus of alcohol-intoxicated rats

김종철,이은용,김호현,김이화,Kim Jong-Chul,Lee Eun-Yong,Kim Ho-Hyun,Kim Ee-Hwa 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.2

목적 : 오가피 약침이 알코올에 의해서 중독된 Sprague-Dawley(S-D)계 흰쥐의 해마 치상회에서 새로운 신경세포 생성 및 NOS발현에 미치는 영향을 조사하였다. 방법 : S-D계 흰쥐에 알코올을(2g/kg) 3일간 연속으로 투여한후, 5일간 인체의 중완혈에 상응하는 부위에 오가피 약침(30mg/kg) 치료를 시행하였다. 치료효과를 관찰하기 위해서 BrdU-면역조직화학 염색법 및 NADPH-d-조직화학염색법을 이용하였다. 결과 : 알코올 처치군에서는 BrdU-양성세포수 및 NADPH-양성세포수가 모두 정상군에 비해서 감소한 반면에 알코올 처치후 오가피 약침으로 치료한 군에서는 BrdU-양성세포수 및 NADPH-양성세포수 모두 알코올 처치군에 비해서 증가하였다. 결론 : 오가피 약침치료가 알코올에 의해서 중독된 S-D계 흰쥐 해마 치상회에서 새로운 신경세포의 생성을 증가시키는 것을 확인하였으며, 그 기전으로 산화질소가 관여할 것으로 사려된다.

Management of pine wilt disease vectoring Monochamus alternatus adults using spray and soil application of Metarhizium anisopliae JEF isolates

김종철,Se Jin Lee,김시현,이미롱,Sehyeon Baek,박소은,김준헌,Tae Young Shin,Jae Su Kim 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

Chemical control is widely used to control the Japanese pine sawyer beetle, Monochamus alternatus, but strong chemical regulations require an environmentally sound management strategy. In this work, we investigated the use of entomopathogenic fungi and their application as a means of practical pest management. Thirty-two diverse species of fungal isolates were assayed against adult pine sawyer beetles using a contact method under laboratory conditions, and four isolates showed over 70% virulence consequently. These isolates, two each of Beauveria bassiana and Metarhizium anisopliae were sprayed on the adult beetles at 1 × 10 7 conidia/ml in plastic containers, respectively. The M. anisopliae-treated adult beetles showed 67% mortality. M. anisopliae isolates JEF-197 and JEF-279 demonstrated dosage-dependent insecticidal activity. Following the laboratory experiments, semi-field trials were conducted in young pine trees under high (RH 94%) and low (RH 35%) humidity conditions. In the high humidity conditions, most of the adult beetles stayed on the top of the branches. When the two M. anisopliae isolates were sprayed on the beetles, they showed ca. 50–70% insecticidal activity 11 days after application. In contrast, in low humidity conditions, the adult beetles tried to move off the branches and onto the soil. When the beetles reached the JEF-197 and JEF-279-treated soil, we measured > 90% insecticidal activity. This work suggests that M. anisopliae was the most virulent entomopathogenic fungus against adult Japanese pine sawyer beetles, and this forest insect could be ecologically controlled by the spray and soil application of the M. anisopliae isolates.

대학행정의 현황과 과제

김종철,Kim, Jong-Cheol 한국대학교육협의회 1983 大學敎育 Vol.2 No.-

단일노즐을 사용한 내부순환 공기리프트 반응기에서 수력학과 액체의 흐름특성

김종철,장서일,손민일,김태옥 ( Jong Chul Kim,Sea Il Jang,Min il Son,Tae Ok Kim ) 한국공업화학회 1997 공업화학 Vol.8 No.5

기체분산기로 단일노즐을 사용한 내부순환 공기리프트 반응기에서 수력학과 액체의 흐름특성을 해석하였다. 실험은 공기-물계에서 기체속도와 반응기의 높이를 변화시키면서 국부지역의 기체체류량과 추적자의 충격-응답곡선을 측정하였다. 실험결과, 약 8 cm/s이상의 기체속도에서 상승관은 기포가 강한 합체를 일으키는 난류흐름을 나타내었고 하강관에서는 균일한 크기의 큰 기포들이 분산된 지역까지의 축방향 높이가 기체속도의 증가에 따라 감소하였다. 그리고 국부지역과 반응기 전체의 평균 기체체류량은 기체속도가 증가할수록 증가하였고 반응기 상부지역의 높이가 증가할수록 감소하였다. 또한 혼합시간은 기체속도보다 반응기 상부지역의 높이에 크게 영향을 받으며 이들이 증가할수록 감소하였다. 상승관과 하강관에서 액체의 흐름은 플러그흐름에 근접하였고 완전혼합흐름으로 볼 수 있는 반응기 상부지역의 크기에 따라 반응기 전체의 액체흐름특성이 크게 변화하였다. 이때 액체의 순환속도는 기체속도가 증가할수록 증가하였고 다른 기체분산기에서 보다 상당히 큰 값을 나타내었다. The hydrodynamics and the liquid flow characteristics were investigated in an internal circulation airlift reactor with a single nozzle as a gas distributor. In an air-water system, the gas holdup in the individual flow zone and the impulse-response curve of tracer were measured at various gas velocities and reactor heights. Experimental results showed that for the higher gas velocity(>about 8 cm/s), the flow behavior of bubbles in the riser was turbulent flow due to strong bubble coalescences and the axial height of dispersion zone of large bubbles having uniform sizes in the downcomer was decreased with increasing gas velocity. And mean gas holdups in the individual flow zone and the reactor were increased with increasing gas velocities and were decreased with increasing heights of the top section of the reactor. Also, the mixing time was larger effected by the height of the top section of the reactor and it was decreased with increasing the height of the top section and gas velocity. Flow characteristics of liquid in the riser andthe downcomer was tend to access to plug flow and the overall flow behavior ofliquid was mainly varied with the size of the top section which it was assumed to beperfect mixing zone. In these conditions, liquid circulation velocities were increased with increasing gas velocities and they were higher than those by using other gas distributors.

제주 현무암지역에서의 취.배수로 해저터널 설계 및 시공사례

김종철,허영회,권중락,고형석,정유채,Kim, Jong-Cheol,Heo, Yeong-Hoe,Gwon, Jung-Rak,Go, Hyeong-Seok,Jeong, Yu-Chae 한국지반공학회 2008 지반 : 한국지반공학회지 Vol.24 No.4

실적 함정의 중량추정 분석 연구

김종철,고용석,김태훈,Kim, Jongchul,Ko, Youngseok,Kim, Taehoon 한국군사과학기술학회 2016 한국군사과학기술학회지 Vol.19 No.4

In this study, the estimating weight data of eight leading ships of Korea navy were processed by comparing with the final weight data of their incline test results statistically, and are analyzed as follows; The maximum criteria of the weight margins of Korea naval ship in the preliminary and detailed design phases seem to be appropriate values, but the minimum criteria would be advisable to update more reasonable values. And, the limitation that the acceptable deviation of lightship weight should be 10 % of the lightship weight on a naval ship's ROC(Required Operational Capabilities) is recommended to be prohibited, because it comes from the weight estimation which has considerable uncertainty and it may also drop off design flexibility. Finally, the SWBS(Ship Work Breakdown System) groups which have larger deviation values in the estimating weight of naval ships are necessary to improve their accuracies, and to upgrade their weight database continuously.

스테로이드 국소 주사 후 발생한 일차 접종 결핵

김종철,박용묘,최종수,김기홍 ( Jong Cheul Kim,Yong Myo Park,Jong Soo Choi,Ki Hong Kim ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.6

We report a case of primary inoculation tuberculosis which ceveloped at the site of an intralesional corticosteroid injection in a 47-year-old male. He had chronic, relapsing nummular dermatitis on his left lower leg 8 years and was treated with intermittent intralesional injection of triamcinolone acetonide. Two weeks ago he developed a single, well-demarcated, 5.0㎝×3.5㎝ sized, indolent, brownish red pustule at the site of the corticosteroid injection. He had no previous history of tuberculosis of the lung or other internal organs. AFB smear from the lesion revealed many acid fast bacilli, and Mycobacterium tuberculosis was cultivated and identified. A histopathologic examination of the lesion showed diffuse dermal infiltration composed of lympho-histiocytes and many neutrophils with necrosis of dermal collagen fibers and many acid fast bacilli were demonstrated on AFB stain.

생산기반기술의 중요성 및 발전방안

김종철,Kim, Jong-Cheol 기술표준원 2003 기술표준 Vol.14 No.-