Comparison of metabolic profiling of Daphnia magna between HR-MAS NMR and solution NMR techniques

김성혜,이수진,이원호,이유진,최주영,이하니,Youzhen Li,하슬빈,김석만 한국자기공명학회 2021 Journal of the Korean Magnetic Resonance Society Vol.25 No.2

Daphnia magna is used as target organism for environmental metabolomics. The metabolome of D. magna was studied with NMR spectroscopy. Most studies used the extract of D. magna, but the reproducibility cannot be obtained using extracted sample. In this study, lyophilized D. magna samples were analyzed with two different 1H NMR techniques, HR-MAS on intact tissues and solution NMR on extracted tissues. Samples were measured three times using 600 MHz NMR spectrometer. Metabolite extraction required more than twice as many D. magna, but the metabolite intensity was lower in solution NMR. In the spectra of HR-MAS NMR, the lipid signal was observed, but they did not interfere with metabolite profiling. We also confirmed the effect of swelling time on signal intensities of metabolites in HR-MAS NMR, and the results suggest that appropriate swelling should be used in lyophilized D. magna to improve the accuracy of metabolite profiles.

간호사의 의료용품 개발 경험에 관한 융합연구

김성혜,이영희,윤옥종 한국융합학회 2017 한국융합학회논문지 Vol.8 No.12

This purpose of the study was to investigate nurses’ experience in developing medical supplies. The participants were six nurses, data were collected by open in-depth interviews from January to December, 2016, and analyzed using thematic analysis. Three themes and six sub-themes were identified. Nurse's experience in medical supplies development were ‘new challenge for better patient care’, ‘the reality of nonprofessional beyond compare’, and ‘difficult but fruitful work’. The participants tried to improve the convenience of nursing work and to minimize the economic burden of patients by medical supplies development. They faced the difficulties as a laymen that a lack of information and knowledge about developing process, but felt worth. Based on these findings, there are necessary for the system and the support of the hospital that can reflect the idea of nurses and education about process on medical supplies development. 본 연구의 목적은 간호사의 의료용품 개발 경험을 이해하고자 시도되었다. 의료용품 개발 경험이 있는 간호사 6명을 대상으로 융합적 접근을 위해 심층면접을 통하여 2016년 1월부터 12월까지 자료를 수집하였고, 자료 분석은 주제 분석으로 하였다. 연구결과 ‘더 나은 환자간호를 위한 새로운 도전’, ‘넘기 어려운 비전문인으로서의 현실’, ‘어렵지만 보람된 작업’이라는 3개의 주제와 6개의 하부주제가 도출되었다. 참여자들은 환자 간호 중 간호업무의 불편함을 인식하고, 환자에게는 안전하면서도 경제적 부담을 최소화하고자 의료용품 개발을 시도하였다. 참여자들은 의료용품 개발에 대한 지식과 정보가 부족함으로 인해 많은 어려움을 겪었으나 보람된 작업이었다고 하였다. 따라서 간호사들의 아이디어를 반영해 줄 수 있는 병원 조직 차원의 지원 및 제도적인 뒷받침과 의료용품 개발과정에 대한 교육이 필요함을 제기한다.

나각(螺角)이란 악기명의 재고(再考)

김성혜 민족음악학회 2022 음악과 민족 Vol.- No.63

This thesis is about finding out whether the name, nagak(螺角), an instrument currently employed for daechwita, is correct and attempting to find a correct name for the instrument. For that purpose, every record that has ‘na(螺)’ and ‘nagak(螺角)’ has been examined. It turned out that ‘na(螺)’, the instrument name, has started to be used since the Goryeo Dynasty. Throughout the 500 years of the Joseon Dynasty till 1902, ‘na(螺)’ was identified as the name for the instrument in the records with illustrations. In particular, ‘na(螺)’ was used with illustration both in Akhakgwebeom published in 1493 and Jongmyouigwe in the late Joseon Dynasty. Therefore, it’s safe to say that ‘na(螺)’ had been the official name for the instrument since 1123 till the early 20th century. The name, ‘nagak(螺角)’ derives from the practice of blowing ‘na(螺)’ and ‘gak(角)’ alternately in the mid Joseon Dynasty. Exactly speaking, it originated from ‘nagakgyochi(螺角交吹).’ However, the existing name, ‘na’, and the one formed around the mid Josun dynasty, ‘nagak(螺角)’ started to be mixed up in the 18th century when the King Jeongjo ruled. For about a hundred years, two names were used together even in the same documents. However, the preferred name was ‘na(螺)’. There was a significant change in the practice as the country went through the Japanese occupation. ‘nagak(螺角)’ started to replace ‘na(螺)’ as the main name for the instrument. It was first seen in Joseonakgweyo compiled by Yiwangjikaakdae in 1917. The reason for this mistake may have resulted either from the lack of expertise on the part of the authors or from the Japanese intervention. It has been almost 100 years since the name ‘nagak’ started to be used. Some people probably think that it’s too much to make a fuss about a name which has been used and accepted for about 100 years. However, a few problems may surface from the misuse of the name. For example, if both ‘na(螺)’ and ‘nagak(螺角)’ found in the documents of the Joseon Dynasty as well as in the Goryeo Dynasty were called ‘nagak’, the distinction between the two different instruments would be difficult, just like ‘nagakgyochwi’, the name of which indicates both instruments, and thus causes confusion. Secondly, the misuse of the name may result in obliterating the existence of ‘gak(角)’ and getting rid of it completely. Thirdly, the names are not consistent in translation. For example, when both ‘na’ and ‘gak’ appear in a document, ‘na’ tends to be translated as ‘sora’. Lastly, the ‘na’ had been used for about 800 years, while ‘nagak’ was for about from 100 to 200 years. If ‘nagak’ is estimated to have been used for 100 years, it is presumed to have been usedsince 1917. If the period when ‘na’ and ‘nagak’ were used together is taken into account, then 100 years more could be added to the estimation, making it 200 years, because both started to be used together from the early 19th century to the early 20th century. In short, the instruments made of conch had mostly been called ‘na’ and, then, far later, it started to be called ‘nagak’ as a nickname. It shouldn’t be overlooked that the original name which has the longest history was replaced by the one made either by those whose expertise were questionable or by the Japanese intervention. However, the aforementioned problem can be simply corrected by restoring ‘na’ to its place. In conclusion, the writer suggests that the name, ‘nagak’, which has been used for 100 years so far, should be corrected to ‘na’.

음악영재 부모교육에 대한 부모와 교사의 요구 비교 분석

김성혜 한국예술종합학교 한국예술연구소 2017 한국예술연구 Vol.- No.18

본 연구의 목적은 음악영재교육에 참여하는 자녀를 둔 부모와 음악영재를 지도하는 교사의 음악영재 부모교육에 대한 인식과 요구를 파악하여 보다 효과적인 음악영재 부모교육 프로그램 개발 방향을 탐색하는 것이다. 요구조사 대상은 서울과 지방 소재 음악영재교육원의 교사 108명과 음악영재교육기관에서 교육을 받는 자녀를 둔 부모 224명이었다. 두 집단의 요구 차이를 파악하기 위해 실시한 독립표본 t-검정 결과, ‘음악영재의 특성’, ‘음악영재교육의 목적’ 및 ‘음악영재교육과정의 내용요소’, ‘음악영재 부모교육의 목적’ 및 ‘음악영재 부모교육의 주제’에서 통계적으로 유의한 차이가 나타났다. 두 집단의 요구 차이를 토대로 향후 음악영재 부모교육 프로그램 개발 및 운영을 위한 제언을 정리하면 다음과 같다. 첫째, 부모교육은 부모의 음악영재의 음악적, 인지적, 정의적, 사회적 특성에 대한 이해를 도와야 한다. 둘째, 부모교육을 통해 부모들이 실제로 음악영재 자녀를 지원하고 양육하는데 활용 가능한 이론적, 실제적 정보가 제공되어야 한다. 셋째, 부모의 진로에 대한 관심이 전공의 가치화와 예술가의 사회적 역할에 대한 관심으로 확대되도록 지원해야 한다. This study aims to investigate ways to develop the program for parents of the musically gifted through a comparative analysis of the needs of 108 teachers and 224 parents. Independent t-test is used to explore the significant statistical differences in the followings: ‘The characteristics of musically gifted’, ‘objectives of gifted education for music’, ‘the content of a curriculum for the musically gifted’, ‘the goals of the education for parents of the musically gifted’, ‘suggested topics in the education for parents of the musically gifted.’ There are some suggestions for further development of the program for parents of the musically gifted. First, the main objective of parent education should be to deepen their understanding of the cognitive, emotional, and social uniqueness of the musically gifted. Secondly, the program for parents should provide more information regarding the musically gifted and gifted education to parents. Thirdly, parents are useful for the career education because of their significant role in creating the value and identity of the musically gifted.

의궤에 보인 준화의 형태와 의미

김성혜,이재옥(논평) 한국음악사학회 2012 학술대회 Vol.12 No.-

산화아연 나노입자가 Poly(p-phenylene benzobisoxazole) 섬유의 자외선 저항성 향상에 미치는 영향

김성혜,이상철,민병길,Kim, Sung Hea,Lee, Sang Cheol,Min, Byung Gil 한국섬유공학회 2018 한국섬유공학회지 Vol.55 No.6

Poly(${\rho}$-phenylene benzobisoxazole) (PBO) and it's nanocomposites with ZnO nanoparicles containing 0.5-2.0 wt% were successfully fabricated in poly(phospheric acid) and $P_2O_5$ via in situ polymerization to investigate the improvement for the UV resistance of PBO fiber. The as-polymerized solutions having 14 wt% of PBO solid content were spun at liquid crystalline state through dry-jet wet spinning to prepare PBO and it's nanocomposite fibers. It was found that the ZnO nanoparticles were dispersed individually in the fibers from SEM observation because of in situ polymerization for a long time up to 33 h. It was concluded that ZnO nanoparticles were significantly effective in enhacing UV resistance of PBO fiber even at low addition amount by exhibiting much lower reduction in tensile strength under the same UV exposure time.

2015 개정 교육과정 고등학교 진로 선택 과목 평가기준 개발

김성혜,박용호 서울대학교 교육종합연구원 2019 The SNU Journal of Education Research Vol.28 No.3

The study aims to develop assessment standards and tools for career-related subjects of high school English. Four career-related electives were newly included in the 2015 National English Curriculum of Korea in order to support students to explore careers or further academic studies. According to objectives and content of the subjects in the National Curriculum, the study develops guidelines on how to evaluate students’ achievement and understand the characteristics of each achievement level. These achievement standards have been developed to provide descriptions to help teachers understand how they should develop evaluation criteria for their classes. The assessment standards are defined as expected levels that indicate what students know and what they can do in terms of ‘high’, ‘intermediate,’ and ‘low’. As for achievement levels for the four language domains of the subject, a three-level grading system of A/B/C is suggested. This is to lessen the burden of evaluation and to have a class in which the main purpose of building English communication skills is well reflected. As for assessments tools, multiple choice and short-answer type questions are avoided while examples of performance assessment for speaking and writing including descriptive writing, oral presentation, and essay writing are suggested. 본 연구에서는 2015 개정 고등학교 영어과 교육과정에서 새로이 편성된 진로 선택 과목에 대하여 학생들이 교육과정의 목표에 도달했는지를 판단할 수 있는 기준으로 활용할 수 있도록 영어 교과 성취기준에 대한 평가기준을 개발하고자 하였다. 우선 교육과정 문서에 제시되어있는 성취기준을 기반으로 평가 활동에서 판단의 기준이 될 수 있는 교육과정 성취기준을 재구성하여 학생들이 학습을 통해 성취해야 할 지식, 기능, 태도의 능력과 특성을 진술하였다. 이를 ‘평가준거 성취기준’이라 하고, ‘평가준거 성취기준’에 도달한 정도를 ‘상’, ‘중’, ‘하’로 나누어 진술하여 ‘평가기준’을 개발하였다. 또한 진로 선택 과목의 영역별 성취수준은 평가의 부담을 줄이고 과목 신설 취지를 반영한 수업이 가능하도록 A/B/C의 3단계로 기술하였다. 예시 평가도구 개발에서는 학교 현장의 요구에 알맞게 활용할 수 있도록 다양한 유형을 제시하되 선다형, 단답형 등의 평가는 지양하고, 서술형, 구술, 논술 등 말하기, 쓰기 수행 평가의 예시를 제시하고자 하였으며, 과정 중심 평가의 예시가 되도록 하였다.

사회복지공무원의 클라이언트 폭력경험과 문제음주 관계에 미치는 외상 후 스트레스의 매개효과

김성혜,윤명숙 알코올과 건강행동학회 2015 알코올과 건강행동연구 Vol.16 No.2

Objectives: The purpose of this study was to examine the mediating effect of Posttraumatic Stress Disorder(PTSD) on the relationship between client violence and problem drinking among social welfare officials. Methods: Subjects of this study were 208 social welfare officials in Jeollabuk do. IES-R-K, AUDIT-K, client violence scale were used for the study. Collected data were analyzed by SPSS 21.0, AMOS 21.0. Structural Equation Modeling in AMOS 20.0 was adopted to examine measurement model and mediating effects of PTSD by using bootstrapping method. Results: First, the results indicated that the incidences of full PTSD and subsyndromal PTSD in the subjects were 30.8% and 23.1%, respectively and total 53.9% showed PTSD. Second, client violence showed significant impact on the problem drinking of subjects. Third, the analysis resulted confined the partial mediation effects of PTSD in the relations between client violence and problem drinking among social welfare officials. Conclusion: Based on these findings suggested that social work officials’ intervention service should be discussed to prevent PTSD, to treat client violence and attention to the PTSD, problem drinking. The implications and limitations of these findings were discussed, and directions for future studies were also proposed.

매킨토시 작품에 나타난 공간표현 특성에 관한 연구

김성혜 한국기초조형학회 2003 기초조형학연구 Vol.4 No.1

찰스 레니 매킨토시는 그의 강연 ‘고상함(Seemliness)'의 내용 중 “현대 예술에서 진정한 형과 색은 감정과 건물 또는 대상의 순수하고 절대적인 요구를 이해함에 의해 만들어진다.”라고 이야기 하고 있다. 이 부분은 그가 영향을 받은 것으로 알려진 존 러스킨의 “건물에 있어서 가장 필요로 하는 것은 적은 예산 속에서도 그 목적에 완벽하고 변함없이 부합되도록 하는 일이다.”란 언급과 비교할 때, 기능에 관한 합일에는 동의하면서도 감정의 문제가 그의 디자인 과정에서 중요한 인자임을 표명하고 있다. 매킨토시는 창조적 상상력을 지닌 예술이 진정한 삶을 깨달을 수 있다고 믿었기에 그는 자연의 외적 형태를 디자인 모티브로 생각하였을 뿐 아니라 예술가의 내적 세계를 반영하는 자극으로 생각하였다. 자연의 분석과 이해를 통해 그는 건축, 가구, 인테리어 디자인에서 예술적 능력과 독창성을 보여주는 그의 스타일을 만들었다. 게다가 그는 현대 디자인 작업을 위해 사회적, 정신적 분석을 그의 스타일에 포함시켰다. 매킨토시 작품들은 장식과 구조적 측면에 미묘한 조화 속에 있는 탁월한 균형감을 보여준다. 그는 장식적 단순성을 인지한 최초의 인물 중의 한사람이었으며, 그의 작품속의 자연의 가능성은 오늘날까지도 신선하고 독창성을 유지하고 있다. Charles Rennie Mackintosh stated "The only true modem individual art in proportion in form and in colour, is produced by an emotion, produced by a frank and intelligent understanding of the absolute and true requirements of a building or object" in his lecture on the subject of 'Seemliness'. This state shows that emotion was one of important factors in his design, and his consideration about function was influenced by Ruskin who instructed "he first thing to be required of a building is that it shall answer its purpose completely, permanently, and at the smallest expense...". Mackintosh believed art is able to realize real life with creative imagination, so he thought nature as not only design motive by its external appearance but also stimulant to reflect inner world of artists. Through analysis and appreciation of nature, he made Mackintosh style that showed originally and artistic ability in architecture, furniture and interior design. In addition to, he included social and psychological analysis in his style to make modern design works. The best of Mackintosh's work displays a subline poise, where decorative and structural aspects are held in delicate balance. He was among the first to conceive decorative simplicity, and the anticipatory nature of his work has kept it fresh and original to this day.

ATM기반 LAN 구축을 위한 MPOA서버 기능 설계 ( Design of the MPOA Server Function for Structuring LAN over ATM )

김성혜,류걸우,강훈 한국통신학회 1998 한국통신학회 학술대회논문집 Vol.17 No.3