Pseudomonas sp. YS-542가 생산하는 Chitinase의 정제

강신욱,최희상 慶北專門大學 2003 慶北專門大學 論文集 Vol.22 No.-

The optimal condition for the production of chitinase from Pseudomonas sp. YS-542 was follow ; 1.0% colloidal chitin, 1.0% tryptone, 0.2% MgSO_(4)7H_(2)O, 0.1% K_(2)HPO_(4), pH 8.0 at 25℃ for 48hrs cultivation. The chitinase was purified by ammonium sulfate fractionation, two times of affinity adsorption. Yield of the purified enzyme was 19.2%. The purified enzyme showed a single protein band on polyacrylamide gel electrophoresis and its molecular weight was estimated to be 47,000 by SDS-polyacrylamide gel electrophoresis.

치위생과 학생들의 B형 간염 바이러스의 표지자와 인지도에 관한 연구

강은주,신상희,장선희 韓國學校保健學會 2002 韓國學校保健學會誌 Vol.15 No.2

This study is aimed at offering basic data about prevention of Hepatitis B virus and infection control to dental hygiene students who will work mostly at dental offices which are characteristically exposed to a high risk of HBV infection. For this study, surveyed were 188 sophomores and juniors of the dental hygiene department who volunteered to undergo the tests of HBsAg and HBsAb. The examination of the blood collected from the samples and analysis of their perception about hygiene resulted in following conclusion: Positive HBsAg Four was found in 4 testees(2.1%) while 118(62.8%) revealed positive. HBsAb. Juniors showed higher positive rate in HBsAb whereas sophomores had higher positive rate in HBsAb. But such difference has statistically no significance. Generally, students seemed to pay little attention to HBV, judging from the survey of their perception about the present state of HBsAb and HBsAb(p>0.05), conduct of infection(p<0.05), completion of 3 requested vaccination(p>0.05), and formation of antibody(p>0.05). With regard to the infection routes of HBV, most students(92.4%) replied "through blood", which is statistically insignificant, though. Next ratio goes to the reply "through contaminated injectors". This reply came more from sophomores than from juniors, a difference which is statistically significant(p<0.05). The lowest rate of possible infection(29.2%) was thought to be "through breast-feeding of a positive mother"(p<0.05). In general, it turned out that sophomores had more knowledge about the infection routes of HBV than juniors. In terms of clinical history among family members, 6(3.1%) reported that some of their family members are currently suffering from a liver complaint, 3(1.6%) replied their family members were once afflicted, and 4(2.1%) said their members died of hepatitis. Except 10(4.7%), all the surveyees replied that their states of health are better than normal. Generally, sophomores are healthier than juniors except for very health case, a difference which is statistically of no value.

14각형 헥사아자 거대고리 구리(II)착물과 음이온의 반응에 미치는 리간드의 치환기 효과

姜信杰,李成得성득,權在槿,鄭守炅 大邱大學校附設 基礎科學硏究所 1991 基礎科學硏究 Vol.8 No.-

Reactions of copper(In complexes of 1, 8-dimethyl-, 1, 8-diethyl-, and 1, 8-dipropyl-1, 3, 6, 8, 11, 13-hexaazacyclotertradecanes(A, B, and C, respectively) with halide and pseudohalide ions have been studied in aqueous solutions by spectrophotometric method. It is observed that the equilibrium constants(K) of these reactions decrease with the ligand structure in the order A> B>C for anions and decrase with the anions in the order SCN^(-)>N_(3)^(-)>Br^(-)>Cl^(-) for complexes. The values of K increased with increasing temperature. Thermodynamic parameters obtained from the equilibrium constants are investigated.

강재 슬릿형 댐퍼를 부착한 철근콘크리트조 골조의 거동

신종익,이정한,송한범,강대언,오상훈,차승렬,이용재,김상대,이원호 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

This study investigates the performance of the reinforced concrete frame with the steel plate slit damper. The test was carried out to investigate the structural behavior between the reinforced concrete bare frame and the reinforced concrete frame with the steel plate slit damper. The test results showed that the energy absorption capacity of the damped-frame was several times larger than that of the bare frame. For the damage by the input energy is concentrated on the steel plate slit damper, the ductilities of the former structure were increased and the damaged frame can be reused after exchange the steel plate slit damper.

백서 혈관평활근 세포에서 α-Lipoic acid가 PAI-1 발현, 세포의 증식, 주유능 및 신생내막 형성억제에 미치는 효과

신동우,이동욱,이상준,김혜순,강효경,안종덕,이인규 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:당뇨병의 혈관 합병증의 발생에 있어서 산화스트레스는 중요한 역할을 하는 것으로 알려져 있다. 혈관 평활근세포에서 고혈당은 안지오텐신Ⅱ와 더불어 활성산소족(ROS)을 증가시키며, 산화스트레스에 민감한 전사인자들을 활성화시켜 동맥경화증을 유발하는 것으로 알려져 있다. 이에 저자들은 항산화제로 알려진 알파­리포산이 혈관 평활근세포의 증식속도와 이주에 미치는 영향과 PAI­1발현에 미치는 영향을 조사하였고 이와 동시에 백서의 혈관 손상 모델을 이용하여 알파­리포산이 신생내막 증식을 억제시킬 수 있는지를 알아보았다. 방법:In vitro 실험은 백서의 대동맥 평활근세포를 고농도의 포도당(22mM)과 100nM의 안지오텐신Ⅱ로 4시간 배양하였다. 알파­리포산을 처리 후, PAI­1 mRNA의 발현을 보기 위해 노던 블롯을 시행하였고 평활근세포의 유주능과 증식속도에 미치는 영향을 보기 위해 성장속도의 유주능 분석을 시행하였다. 또한 평활근세포의 유주능에 NF­μB 경로가 미치는 영향을 보기 위해 겔 지연 분석과 NF­μB 보고 유전자 분석을 시행하였다. In vivo 실험으로 백서의 혈관손상 모델을 이용하여 알파­리포산 처리 후 혈관의 신생내막 두께를 비교하였다. 결과:In vitro 실험에서 고농도 포도당과 안지오텐신Ⅱ에 의해 유도된 PAI­1 mRNA 발현증가는 알파­리포산 처리에 의하여 용량에 비례하여 억제되었다(p<0.05). 알파­리포산 처리로 혈관 평활근세포의 유주능은 유의하게 억제되었으나(p<0.01),증식속도는 유의하게 억제되지 않았다. 또한 알파­리포산 처리로 NF­μB 발현도 유의하게 억제되었다(p<0.01). In vivo 실험에서 알파­리포산을 주입한 군에서 혈관손상에 의한 신생내막의 증식이 유의하게 억제되는 것을 보여주었다(p<0.01). 결론:알파­리포산은 백서의 대동맥 평활근 세포의 증식속도는 억제하지 못했으나 유주능은 유의하게 억제하였으며, 혈관 손상 모델에서 신생 내막 증식도 유의하게 억제함을 확인할 수 있었다. 이러한 작용은 알파­리포산에 의한 NF­μB 경로의 억제와 연관이 있는 것으로 사료된다. Background : Exposure to large amounts of glucose causes a characteristic dysfunction and morphologic changes of the endothelium by an increased production of reactive oxygen species(ROS) in diabetes. The plasminogen activator inhibitor-1(PAI-1), which modulates fibrinolysis and cell migration may influence proteolysis and neointimal formation in vascular smooth muscle cells(VSMC). Antioxidants have been proposed to inhibit multiple proatherogenic events. This study investigated the effect of α-lipoic acid on PAI-1 expression and VSMC proliferation and migration both in vivo and in vitro. Methods : In the in vitro study, cultured rat aortic smooth muscle cells(RASMC) were incubated in a medium containing high glucose (22 mM) and 100 nM angiotensin Ⅱ for 4 hour. After α-lipoic acidtreatment, a -migration and growth assay of the RASMC, and a gelmobility shift assay and reportergene analysis for nuclear factor- иB(NF- иB) and northern blot analysis for PAI-1 were performed. In the in vivo study, the effect of α-lipoic acid on neointimal hyperplasia in a rat carotid balloon injury model was evaluated. Results : RASMC migration was inhibited significantly by α-lipoic acid (p<0.01), but their prolife ration was not inhibited. The NF-иB DNA binding activity and NF-иB promoter activity was inhibited by α-lipoic acid significantly (p<0.01). α-lipoic acid inhibited PAI-1 mRNA expression by high glucose and angiotensin Ⅱ in dose dependent manner (p<0.05). In the rat carotid artery balloon injury model, neointimal formation was reduced by α-lipoic acid treatment in a dose dependent manner significantly (p<0.01). Conclusion : α-lipoic acid suppresses migration, but not prolife ration in RASMC. α-lipoic acid also reduce neointima formation in a rat carotid balloon injured model. This effect might be related to the blocking of NF-иB which increase the expression of the genes associated with atherosclerosis including TNF-α, IL-1, IL-6, endothelin-1, MCP-1, VCAM-1, ICAM-1, E-selectin, tissue factor(J Kor Diabetes Asso 25:446~459, 2001).

에어볼 발파공법의 현장적용 사례연구

강대우,이신,김상욱 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

발파로 인한 환경적 문제는 주로 진동과 폭음에 대한 영향이나, 발파로 인한 파쇄도 및 경제성에 관해서도 효율적인 발파공법들이 많이 개발되고 있다. 이러한 문제를 해결하기 위한 방법으로 선진국에서 많이 사용되는 방법이 air decking을 이용하는 발파방법이다. 이미 선진국의 발파현장에서는 air decking 기술을 이용한 다양한 발파방법으로 VARI-STEM, GAS-BAG, POWER DECK 등이 이용되어지고 있다. 본 연구에서는 이들 중에서 Air ball 제품을 이용한 발파공법에 대하여 연구하고 국내 현장적용과 효과에 대하여 분석하였다. 그 결과 발파진동의 경우에는 약 30 ~ 40% 정도의 감쇄효과가 나타났고, 화약량의 경우 20 ~ 25%정도의 감소를 보였다. 또한 폭음의 경우에도 Air ball을 이용한 발파가 효과적이었으나 분석결과의 결정계수가 낮아 이는 더 많은 실험을 통하여 다시 분석해야 할 것으로 생각된다.

유기박막 광도파로형 바이오(칼슘이온)센서의 개발

강신원,박이순,김성훈,김창원 경북대학교 센서기술연구소 1955 연차보고서 Vol.1995 No.-

본 연구는 고감도, 빠른 응답특성 및 넓은 센싱범위를 갖는 능동형 유기박막 광도파로형 칼슘이온센서를 개발하였으며, 이와 병행하여 중성 ionophore와 함께 특정 음이온 선택성의 색소(LAD : lipophilic anionic dye)를 설계·합성을 하였다. 유기박막 광도파로형 칼슘이온센서는 감지막 자체를 박막광도파로화 함으로써 빠른 응답시간과 고감도의 특성을 가졌으며, 514㎚ 광원에서 10^-6∼1M의 범위의 Ca^++ 의 농도를 측정할 수 있었다. 또한 새로이 합성된 LAD의 칼륨이온선택성을 분광측정으로 평가한 결과, 광이온센서(optode)들의 변색성 이온감응물질로서 사용이 가능함을 확인하였다. A new approach to the design and construction of thin-film optical waveguide Ca^+2 sensor has been developed by using a copolymer matrixed membrane that incorporates a cation-selective neutral ionophore, H^+-selective neutral chromo-ionophore, and lipophilic anionic sites in the same plasticized copolymer membrane. This sensor is based on absorption measurement, and it makes use of host molecules that change their optical properties through an actual ion-sensitivity recognition process. In parallel, the high ion-selective lipophilic anionic dye(LAD) was synthesized and evaluated. Proposed ion sensor, utilizing thin film membrane which is the waveguide itself, exhibited rapid response time and high sensitivity. And sensor could detect a wide range of Ca^++ concentrations ranging from 10^6 ∼ 1M by measuring the absorbance change at 514㎚ of light. The newly synthesized LAD showed its potential applicability as chromoionophore for optodes and/or thin-film optical waveguide sensors.

태권도 나래차기 기술의 운동학적 특성분석

신성휴,강성철 龍仁大學校 武道硏究所 1998 武道硏究所誌 Vol.9 No.1

The objective of the study is to search the mechanical principles in Naraechagi movement. This kicking techniques consists of complicated highly demanding movements. For the goal of the study, three dimensional film analysis were conducted. The movement were diveded into 3 phases, they are: phaes 1-left leg take off, phaes 2-left foot impact, phaes 3-right foot impact. Variables analyzed are movement duration time for each phase, locations of center of gravity, velocity of center of gravity, thigh, foot, joint angle of ankle, knee, hip. Followings are the result: 1. Performance time of Nele-Chagi is 0.54±0.005 second form taking off the left foot to the impact of the left foot and 0.24±0.03 second from the impect of left leg to the impect of right foot, therefore total required time is 0.79±0.05 second 2. The enter of body's position is drawn a hyperbolic gentle curve that is like close from the land and then it occurs impact immdiatly after reaching the highest point from arrargement motion for impact of the right foot. 3. The highest speed of segment is form of whipping motion by conservation of angular momentum rule that is important alternative motion of round ; left and right side of foot's speed also occur the highest. 4. To deliver momentum of legs to the target is that you should do large of an angle of hip joint and use elesticity of the waist becouse et occur the highest speed of knee extemsion by small and angle of knee.

날씨가 주가에 미치는 영향

신민식,강성미 慶北大學校出版部 2004 經商論集 Vol.32 No.1

Based on psychological evidences, which show that weather affects mood, and this could also affect decision-making, we analyzed correlations between cloud cover and the daily composite index within Seoul, Daejeon, Daegu, Gwangju, Busan which are representative cities from Korea. Evidence demonstrates the following. First, analysis supports the correlation between cloud cover data and the daily composite index and shows that cloud cover affects stock returns negatively except for those in Daejeon. Second, we study the correlation between cloud cover of days when it has no rain and the daily composite index shows that Gwangju is not positive anymore. It has reverse effects particularly for Daejoen, as the data indicates opposite results. Third, the correlation between new cloud cover figures are weighted with GRDP data and the daily composite index show that cloud cover is significantly correlated with negative stock returns.

驅動部 動特性을 高慮한 可變構造 制御 알고리듬

강익호,김정욱,신휘범,이정훈,고상호 慶尙大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.13 No.-

In this paper, the dynamics of actuators for generating the input of plants is considered in a design of the variable structure systems. While the input for plants is usually implemented by means of a certain actuator, the actuator dynamics is not incorporated in most of the VSS researches until now. The control algorithm of a VSS incorporating actuator dynamics is presented. and the simulation is given the usefulness of the algorithms.