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Pseudomonas sp. YS-542가 생산하는 Chitinase의 정제
강신욱,최희상 慶北專門大學 2003 慶北專門大學 論文集 Vol.22 No.-
The optimal condition for the production of chitinase from Pseudomonas sp. YS-542 was follow ; 1.0% colloidal chitin, 1.0% tryptone, 0.2% MgSO_(4)7H_(2)O, 0.1% K_(2)HPO_(4), pH 8.0 at 25℃ for 48hrs cultivation. The chitinase was purified by ammonium sulfate fractionation, two times of affinity adsorption. Yield of the purified enzyme was 19.2%. The purified enzyme showed a single protein band on polyacrylamide gel electrophoresis and its molecular weight was estimated to be 47,000 by SDS-polyacrylamide gel electrophoresis.
Chitinase를 생산하는 Pseudomonas sp. YS-542의 분리 및 특성
강신욱,이준우,방광웅,최희상 慶北專門大學(영주경상전문대학) 2002 慶北專門大學 論文集 Vol.21 No.-
A chitinase-producing bacterium was isolated from soil by selective enrichmentment culture medium. The bacterium was identified as Pseudomonas sp. based on the data obtained from its morphological, cultural and biochemical characteristics. The isolated strain was named Pseudomonas sp. YS-542. The medium for the production of chitinase was consisted of colloidal chitin 1.0%, tryptone 1.0%, MgS04·7H2O 0.2%, K2HP04 0.1%. The optimum cultural temperature, initial pH and time for the best production of chitinase were 25℃, 48hrs and pH 8.0, respectively.