환자의 비강으로부터 분리된 메티실린 내성 황색 포도알균의 분자 아형 분석

Sang-Ha Kim,Sung-Bae Park,Heechul Park,Jun Seong Kim,Jungho Kim,Jiyoung Lee,임재원,Young Kwon Kim,Sunghyun Kim 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.2

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infections in different parts of the body and causes skin and soft tissue infections (SSTI). The present study examined the antimicrobial resistance patterns and molecular epidemiological characteristics of MRSA isolated from nasal swabs in clinical patients. SCCmec type of MRSA isolates from clinical patients were analyzed: 24 cases were SCCmec type-II; two cases were type-II/IVa; one case was type-II/V; one case was type-IVa; 11 cases were not-typeable. The mec complex type of MRSA isolates from clinical patients were analyzed: 29 cases were mec complex type A, and 10 cases were not-typeable, but type B was not found in the present study. In conclusion, SCCmec type-II and mec complex type A were the most dominant MRSA subtypes among the MRSA isolates from a nasal swab of patients, and the results were similar to other studies on hospital-acquired MRSA (HA-MRSA). These results can not only provide basic data for hospital infection management but also be a good guideline for MRSA infections in the Republic of Korea. 본 연구에서는 환자의 비강으로부터 분리 배양된 총 39건의 MRSA 분리 배양 균주를 이용해 mecA 유전자 검출, SCCmec typing과 mec complex typing을 분석해 보고자 하였다. 임상 환자의 비강으로부터 분리 배양된 MRSA 총 39주 중 SCCmec type-II가 24건, type-II/IVa가 2건, type-II/V가 1건, type-IVa가 1건, not-typeable이 11건으로 분석되었으며, mec complex type A가 29건, not-typeable이 10건이었으며, type B는 없는 것으로 분석 되었다. 결론적으로, 환자의 비강으로부터 분리된 MRSA 분리 배양 균주 중 SCCmec type-II와 mec complex type A 아형이 가장 많이 분포하고 있었으며, 이 결과는 의료기관 획득 MRSA에 관한 다른 연구결과와 유사한 결과를 나타냈다. 이후 환자의 비강으로부터 분리 배양된 not-typeable 아형의 MRSA 균주를 대상으로 국내에서 발견되는 새로운 MRSA 아형 규명에 관한 추가 연구가 필요할 뿐만 아니라, MRSA 분리 배양 균주의 아형을 분석함으로써 그 분자적 특성을 분석한 결과를 바탕으로 병원 감염관리를 위한 기초자료를 제공할 수 것으로 사료된다.

Bloodstream Infections and Clinical Significance of Healthcare-associated Bacteremia: A Multicenter Surveillance Study in Korean Hospitals

Son, Jun Seong,Song, Jae-Hoon,Ko, Kwan Soo,Yeom, Joon Sup,Ki, Hyun Kyun,Kim, Shin-Woo,Chang, Hyun-Ha,Ryu, Seong Yeol,Kim, Yeon-Sook,Jung, Sook-In,Shin, Sang Yop,Oh, Hee Bok,Lee, Yeong Seon,Chung, Doo The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.7

<P>Recent changes in healthcare systems have changed the epidemiologic paradigms in many infectious fields including bloodstream infection (BSI). We compared clinical characteristics of community-acquired (CA), hospital-acquired (HA), and healthcare-associated (HCA) BSI. We performed a prospective nationwide multicenter surveillance study from 9 university hospitals in Korea. Total 1,605 blood isolates were collected from 2006 to 2007, and 1,144 isolates were considered true pathogens. HA-BSI accounted for 48.8%, CA-BSI for 33.2%, and HCA-BSI for 18.0%. HA-BSI and HCA-BSI were more likely to have severe comorbidities. <I>Escherichia coli</I> was the most common isolate in CA-BSI (47.1%) and HCA-BSI (27.2%). In contrast, <I>Staphylococcus aureus</I> (15.2%), coagulase-negative <I>Staphylococcus</I> (15.1%) were the common isolates in HA-BSI. The rate of appropriate empiric antimicrobial therapy was the highest in CA-BSI (89.0%) followed by HCA-BSI (76.4%), and HA-BSI (75.0%). The 30-day mortality rate was the highest in HA-BSI (23.0%) followed by HCA-BSI (18.4%), and CA-BSI (10.2%). High Pitt score and inappropriate empirical antibiotic therapy were the independent risk factors for mortality by multivariate analysis. In conclusion, the present data suggest that clinical features, outcome, and microbiologic features of causative pathogens vary by origin of BSI. Especially, HCA-BSI shows unique clinical characteristics, which should be considered a distinct category for more appropriate antibiotic treatment.</P>

Suppression of lung inflammation by the methanol extract of <i>Spilanthes acmella</i> Murray is related to differential regulation of NF-κB and Nrf2

Kim, Kyun Ha,Kim, Eun Jung,Kwun, Min Jung,Lee, Ji Yeon,Bach, Tran The,Eum, Sang Mi,Choi, Jun Yong,Cho, Sayeon,Kim, Sang-Jun,Jeong, Seung-Il,Joo, Myungsoo Elsevier 2018 Journal of Ethnopharmacology Vol.217 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Although <I>Spilanthes acmella</I> has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of <I>S. acmella</I> Murray (SAM).</P> <P><B>Materials and method</B></P> <P>The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.</P> <P><B>Results</B></P> <P>The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.</P> <P><B>Conclusion</B></P> <P>Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of <I>S. acmella</I>.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

자연환경방사선에 의한 피폭선량(유효선량당량)의 종합적 해석연구

전재식,채하석,이병영,김현창,이동준,김도성,엄두하 충남대학교 기초과학연구소 1990 연구논문집 Vol.10 No.-

자연환경방사선의 세 가지 주요 요소인 지각방사선, 우주선 전리성분 및 라돈과 그 자핵종이 주는 선량을 측정, 해석하였는 바 18개월간에 걸쳐 대전, 수원, 경산 지역에 대하여 수행하고 이를 종합하여 유효선량당량으로 평가하였다. 지각방사선과 우주선 전리성분 측정을 위하여 3"∮×3" Nal(Tl) 섬광검출기와 휴대형 다중파고분석기를 이용한 감마선 분광분석 및 ^7LiF PTEE disk TLD에 의한 적산선량측정을 병행하였고, 공기중 라돈농도의 측정은 CR-39 고체비적검출기를 내장한 라돈컵을 설계제작하여 사용하였으며 비적수 라돈농도 교정인자는 스웨덴 국립방사선 방호연구소의 표준 라돈 챔버내에서 조사하여 결정하였다. 세 지역의 가연방사선에 의한 유효선량당량에는 약간의 수치적 차이는 있었으나 본질적 차이는 찾아볼 수 없었으며 지역 측정치의 분산가중 평균치는 지각방사선 (463±3)uSvㆍy^-1[(52.9±0.3)nSvㆍh^-1], 우주선 전리성분 (287±15)uSvㆍy^-1[(32.8±1.7)nSvㆍy^-1]로 외부 피폭이 (775±9)uSvㆍy^-1[(88.5±1.0)nSvㆍy^-1]였으며 라돈과 그 자핵종에 의한 내부피폭은 (1973±298)uSvㆍy^-1[(225.2±34.0)nSvㆍy^-1]였다. 따라서 자연황경방사선에 의한 여간 총 선량당량은 (2762±296)uSvㆍy^-1[(315.3±34.0)nSvㆍh^-1]로 평가되었는데 이 값들은 UN보고서^(11)에 나온 “정상적 자연방사선 지역”의 값과 비교할 만한 타당한 값으로 나타났다. Assessment of effective dose equivalent from natural environmental radiation that consists of terrestrial gamma­ray, ionizing component of cosmic­ray and alpha­rays of atmospheric radon and its decay products has been carried out for 18 months in Daejeon, Suwon and Kyungsan areas. A 3"∮×3"cylindrical Nal(Ti) scintillation detector in association with portable multichannel analyser and ^7LiF PTFE disk TLDs were used for the measurement of terrestrial gamma­ray and cosmic­ray ionizing component, while self­designed radon­cups with CR-39 SSNTD attached to the inside bottom were used for the integral measurement of at­mospheric radon concentration. Determination of calibration factor for converting track density of CR-39 detector to radon concentration was made by irradiation of radon­cups in the standard radon chamber of Swedish National Institute of Radiation Protection. Although resultant effective dose equivalents of three different measurment points show minor numerical differences, no essential discripancy between the places was found. The variance weighted means of the annual effective dose equivalents of each component of radiation from the tree places came out to be: (463±3)uSvㆍy^-1[(52.9±0.3)nSvㆍy^-1] for terrestrial gamma­ray, (287±15)uSvㆍy^-1[(32.8±1.7)nSvㆍy^-1] for ionizing component of cosmic­ray and (1973±298)uSvㆍy^-1[(225.2±34.0)nSvㆍy^-1] for internal exposure of radon and its decay products. Consequent total effective dose equivalent turned out to be (2762±298)uSvㆍy^-1[(315.3±34.0)nSvㆍy^-1], which if reasonably comparable with that appeared in UNSCEAR Report^[11]as per caput annual effective dose equivalent in “areas of normal back ground radiation.”

마이크로파 용융삽입법을 이용한 생분해성 나노복합체의 제조

하원조,신준식,송승욱,김준호,손세모,박성수 한국환경과학회 2004 한국환경과학회지 Vol.13 No.4

The purpose of this study was to investigate the possibility of application of microwave energy for the fabrication of polymer/clay nanocomposite. APES/Clay nanocomposites were prepared at 130℃ for 30min with various content of clay by melt-intercalation method under classical and microwave heating source. APES/Clay samples were characterized by the means of X-ray diffractometry(XRD), thermal gravimetric analysis(TGA), and rheometric dynamic analysis(RDA). It was found that intercalated or exfoliated state was obtained in the samples according to the condition of organic modification, clay content, and heating source.

마이크로파 여기 플라즈마광 생성을 위한 도파관 시스템 설계에 관한 연구

전상재,전후동,송창현,하석영,이승혁,이태호,박의준 금오공과대학교 2005 論文集 Vol.26 No.-

In this paper, the plasma lighting system(PLS) excited by the commercial high power magnetron is developed. The design concepts are based on maximizing the huninous efficacy in conjunction with the miniaturization of waveguide system Furthermore the fine tuning is simplified by using only one stub, and the impedance matching is maximized by introducing the tapering technique. The experimental results show that the luminous efficacy can be dramatically improved by the proposed design method.

생쥐 중이와 상기도 점막의 섬모세포운동

정하원,오승하,유원석,이강수,모지훈,전상준,김인상,김종선 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.9

유전자 재조합 사람 IFN-beta인 제네베타와 베네세린의 생물학적 활성의 비교

하종천,이협준,박봉환,김진숙,유대근,남상윤 전주대학교 자연과학종합연구소 2003 전주대학교 자연과학연구소 학술논문집 Vol.15 No.1

Background: Recombinant IFN-beta products have been approved for the treatment of several diseases. Recently, Inbionet, Co. has successfully developed rhIFN-beta product, Genebeta. This study was performed in a purpose of confirming the biological activity of Genebeta by comparison with another recombinant IFN-beta, Beneserin which was already approved. Methods: In vitro antiporliferative activity, regulation of MHC class I expression, antiviral activity, regulation of natural killer cell cytotoxicity were studied for functional comparison of these two rhIFN-beta products. Results: Proliferation assay data showed that two recombinant IFN-beta inhibited T cell and Daudi cell proliferation to a similar extent. When antiviral activity was compared, there was no significant difference between them. The treatment of these two rhIFN-beta also resulted in a similar level of upregulation of class I expression on the surface of A549 cells. Cytotoxicity experiment against tumor cells revealed that both of rhIFN-beta could enhance NK cell cytotoxicity to comparable level. Conclusion: All comparison data indicated that rhIFN-beta products, Genebeta and Beneserin have comparable potentials as a biological response modifier.

실내사무환경의 환경성담배연기(ETS)의 지표물질에 관한 연구

하권철,백남원,박동욱,윤충식,김원,최상준,박지영,최인자,김신범,강태선 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.2

The purpose of this research is to evaluate indoor office environment using the concentrations of nicotine, 3-enthenyl-pyridine(3-EP), and respirable suspended particulate(RSP), which are indicators for environmental tobacco smoke(ETS) and the correlations between indicators and environmental conditions(smoking density, smoking index). The mean air charge per hour (ACH) in smoking rooms was 10.4 and most of the smoking rooms showed non-compliance withe ASHRAE standard value except only one smoking rooms. The concentrations of RSP, 3-EP, nicotine showed log=normal distributions, and became different statistically depending on smoking condition(p〈0.01). The geometric mean concentration of RSP in smoking room was 441.7 ug/㎡ that is far exceeded environmental standard(150 ug/㎡). This implies that fine particulate in smoking room should be carefully controlled considering smoking density and ventilation fate. The mean concentrations of nicotine and 3-EP were 93.4 ug/㎥, respectively. The correlation coefficients between RSP and SI, 3-EP and SI, and Nicotine and SI were 0.67, 0.84 and 0.74, respectively. The correlation coefficient between nicotine and 3-EP, nicotine and RSP, and RSP and 3-EP were 0.76,0.78 and 0.57 respectively.

자연방사선량중 ^222 Rn 기여성분 정량해석

전재식,오희필,채하식,이병영,김도성 충남대학교 기초과학연구소 1989 연구논문집 Vol.9 No.-

옥외 라돈이 호흡기관에 주는 선량을 측정·평가할 목적으로 CR-39 비적검출기를 내장한 라돈 컵을 사용하여 대기중 라돈농도를 측정하였다. 직접형 검출기 및 개방 컵과 필터 컵의 구조를 갖는 CR-39 비적검출기에 대한 라돈검출인자는 공기중의 농도가 잘 알려진 표준라돈 조사시설에서 이들 검출기와 라돈컵을 일정기간 조사하여 결정하였다. CR-39를 70℃, 30% NaOH용액으로 220분간 화학부식하였을 때 직접형 검출기와 개방 컵, 필터 컵에 대한 라돈검출인자는 각각 0.273, 0.0813, 0.0371 tr㎜^-2/(37Bqm^-3·d)였다. 또한 1988년 5월에서 1989년 3월까지 대전(충남대학교)에서 측정한 대기중의 라돈농도는 개방 컵에 의한 결과는 2.74-18.87Bq/㎥(0.07-0.5pCi/ℓ)로서 연평균 8.25Bq/㎥(0.22pCi/ℓ)이었으며, 필터 컵에 의한 결과는 1.67-19.76Bq/㎥(0.05-0.54pCi/ℓ)로 연평균 8.18q/㎥(0.22pCi/ℓ)이었다. 그리고 대기중의 라돈농도에서 ^(220)Rn의 기여성분은 무시할 수 있을 정도인 것으로 판단되었다. 측정한 옥외 대기중의 라돈농도와 부위별 폐선량모형으로부터 ICRP 표준인의 호흡기관에 대한 유효선량당량률은 약 60nSv/h(0.5mSv/y)로 평가되었다. A study for the assesssment of dose given by outdoor radon to respiratory system has been carried out by making use of radon-cups containing CR-39 plastic track detectors. Detection efficiencies were determined by irradiation of the radon-cups in a standard radon chamber of known concentration. Thus determined detection factors of CR-39 plastic track detedtor in bare, open cup and filtered cup geometry are found to be 0.273, 0.0813 and 0.0371tr㎜^-2/(37Bqm^-3·d) respectively, which are chemically etched in 70℃, 30% NaOH for 220 minutes. The outdoor radon concentrations measured at Taejeon(Chungnam National University) from May 1988 to March 1989 are in the range of 2.74-18.8Bq/㎥(0.07-0.51pCi/l) by open cup and 1.67-19.76Bq/㎥(0.05-0.54pCi/l.) by filtered cup. The annual averaged value of the measured outdoor radon concentration is 8.2Bq/㎥(0.22pCi/l). Corresponding effective dose equivalent rate to respiratory system of ICRP standard man is assessed to be 60nSv/h or 0.5mSv/y.