Chiral Separation of (±)-Higenamine by Capillary Electophoresis

Choi,One-Kyun,Jung,Kyo-Soon,Choi,Heisook-Yun,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Higenamine [1-(4-hydroxy-6, 7-dihydroxy-l, 2, 3, 4-tetrahydroisoquinoline) is a cardiotonic constituent of Aconiti tuber, one of the most widely prescribed oriental medicines. S-(-)higenamine was reported to have a stronger cardiotonic activity than R-(+)-higenamine and known as a central intermediate in the biosynthesis of various benzyl isoquionoline alkaloids in plants. The separation of higenamine enantiomers has been accomplished with capillary electrophoresis using cyclodextrins (CDs) as chiral selectors. Good resolution of this enantiomers was obtained using a 50 mM sodium phosphate buffer containing hydroxypropyl β-CDs using 27 cm fused silica capillary (50㎛ i.d., 20 cm to detector) at 25℃. With the electric field of 340 V/cm, the separation time of higenamine enantiomers was less than 6 min. Under this optimum conditions, the relative standard deviations of migration time and peak area were less than 1.6% and 3.2%. A 512-channel diode array detector was confirmed for the higenamine. The detection limits (S/N = 3) of these enantiomers are 1.5mutextrmm/mL. We confirmed the chiral form of higenamine in medicinal plants.

Chemical Composition and Phytoestrogen Analysis of Iranian Black Pomegranate Juice Concentrate and Seeds

Choi,One-Kyun,Kim,Yong-Seong,Yu,Hye-Kyoung,Lee,Chan,Bang,Hyo-Pil,Yang,Deok-Chun,Kim,Young-Kee 한국자원식물학회 2003 Plant Resources Vol.6 No.1

In this study, as preliminary research for the development of natural estrogen supplement the chemical properties of Iranian black pomegranate juice concentrate and seeds were evaluated. Proximate compositions of pomegranate juice concentrate and seeds were as follows; crude lipid 0.4% and 8.2%, moisture 39.9% and 6.6%, crude protein 0.9% and 12.2%, ash 1.4% and 1.7%, and carbohydrate 42.0% and 84.5% respectively. Major amino acids are glutamic acid (1310.0ppm) and aspartic acid (896.2ppm) in juice concentrate, and glycine (611.1ppm) and arginin (401.6ppm) in seeds. Ascorbic acid has the highest concentration of 20.0mg/l00g in juice concentrate and 0.23mg/l00 in seeds. The compositions of unsaturated fatty acids such as linoleic acid and linolenic acid were higher than those of saturated fatty acids such as stearic palmitic acid. Major minerals were potassium, calcium and sodium, potassium was highest in both juice concentrate and seeds. Vitamins were composed of ascorbic acid (20.0mg/l00g), vitamin B$_1$ 수식 이미지(0.12mg/100g) and niacin (0.80mg/l00g) in juice concentrate, and only ascorbic acid(0.23mg/l00g) in seeds. Organic acids such as citric and L-malic acid were detected only in pomegranate juice concentrate. The contents of total polyphenols were 4.55g/L in juice concentrate and 3.5mg/l00g in seeds, respectively. Phytoestrogens detected in pomegranate juice concentrate and seeds were daidzein, quercetin, genistein and 17β-estradiol.

이란산 흑석류 농축액과 그 제품의 성분 및 함유된 Phyto 에스트로겐류에 관한 연구

최원균,정교순,조규성,황명오,유영숙 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.2

천연 호르몬 보충 제제의 개발을 위한 기초 연구로 이란산 흑석류 과즙 농축액과 이를 이용한 제품의 화학성분들을 분석하였다. 석류 농축액의 일반성분은 수분 39.3%, 조지방 0.4%, 조단백질 0.9%, 조회분 1.4%, 그리고 탄수화물은 42.0%이었다. 아미노산 함량은 글루탐산이 1310.Oppm, 아스파르트산이 896.2ppm, 아르기닌이 877.7ppm, 페닐알라닌이 57.5ppm순으필 무기 성분들은 철분 6640.Oppm, 염소 3464.Oppm, 칼륨 2550.8ppm, 인 150.Oppm, 칼슘 80.Oppm 순으로 많이 함유되 어 있었다 비타민은 5가지의 수용성 비타민이 함유되어 있었으며 그 중 비타민 C(20mg/100g)를 제외한 나머지 비타민들이 아주 적은 양이 있었다. 주요 지방산은 palmitic acid(8.3%)와 stearic acid(69.4%)로 전체 지방산의 약 60~80%를 차지하고 있었다. 또한 6종의 phyto및 에스트로겐류가 들어 있었으며 각각daidzein 23.72ppm, quercetin 9,75ppm, catechin 1.48ppm, genistein 0.29ppm, 2,3-di-MeO-estradiol이 0.04ppm, 그리고 17β-에스츠라디올이 0.15ppm이 함유되어 있었다. 이상의 결과들을 석류 농축액으로 제조한 제품 포에버 120과 칡과 대두 isoflavon 농축분말과 비교하다. Phytoestrogens are non-steroidal compounds found in a variety of plants, which exert estrogenic effects in animals. In this study, the physico-chemical properties of Iranian black pomegranate extract and its products as preliminarily research for the developing of natural estrogen supplement were evaluated. The chemical components of Iranian black pomegranate extracts and its product (Forever 120) were analyzed. Proximate compositions of pomegranate extracts were as follows; crude lipid 0.4%, crude protein 0.9%, crude ash 1.4% and carbohydrate 42.0%. Major amino acids of pomegranate extracts are glutamic acid (1310.Oppm), aspartic acid (896.2ppm), arginine (877.7ppm) and phenylalanine (57.5ppm). Fatty acid compositions of pomegranate extract lipid extracted by chloroform-methanol (2:1) were myristic (13.1%), stearic (69.4), oleic acid (6.8%) and palmitic acid (8.3%). Mineral elements were ferrous (6640.Oppm) and potassium (2550.8ppm). Vitamins were composed of ascorbic acid(20.Omg/100g), Vit. B_1 (0.12mg/100g) and niacin (0.80mg/100g), 20 phytoestrogens and 20 estrogens of pomegranate extracts were detected Daidzein (0.29ppm), quercetin (9.75ppm) genistein (0.29ppm) and 17 β-estradiol(0.15ppm). Above the chemical components of pomegranate extracts were compared with that of pome granate its product or other isoflavon concentrates.

국내산 식물의 항균활성 검색

최원균,김용성,조규성,성창근 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.4

한국에서 자생하는 125종의 식물에서 추출한 141가지 메탄올 추출물들에서 식품부패미생물인 Bacillus subtillis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes 그리고 Pseudomonas aeruginosa에 대한 항균 활성도를 조사하였다. Bacillus subtillis에 대해 가장 강한 항균력을 보인 것은 오미자였으며 staphylococcus aureus 에 대해서 대황이, Pseudomonas aeruginosa에 대해서는 산사가 가장 강한 항균력을 나타냈다. 금은화는 Bacillus subtilis를, 산사는 Stophylococcus aureus를, 모과와 황금은 Pseudomonas aeruginosa를, 모감주나무꽃, 밤나무꽃, 장미꽃과 파꽃은 Enterobacter aerogenes를, 오미자는 Escherichia coli를 제외한 나머지 4가지 균주에 모두 항균력이 있었다 141 methanol extracts from 125 plant species which populate in Korea were screened for antimicrobial activity against various food-borne pathogens and food spoilage microorganisms. Those plants were selected from 3 different plant groups : traditional herbs, edible plants and flowers. The methanol extracts were tested by using the disk diffusion assay against five bacteria : Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli. From the evaluation of the inhibition zone diameter of microbial growth, the most significant antimicrobial activity against bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli was observed from the extract of Schizandra chinensis (Turcz) Baill., Rheum officinale Baill., Schizandra chinesis (Turcz) Baill., Koelreuteria paniculata Lax and Crataegus pinnatifida Bunge, respectively. The extract from many plants - Koelreuteria paniculata Lax, Chaenomeles sinensis Koehne, Scutellaria bacicalensis Georgi, Castanea crenata Sieb. et Zucc., Rosa centifolia L;, Allium fistulosum L. var. giganteum Makino, Crataegus pinnatifida Bunge, Schizandra chinensis (Turcz) Baill., Lonicera japonica - showed antimicrobial activity all four tested bacteria.

어유에서 요소부가법에 의한 EPA와 DHA의 농축

최원균,조재선 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-

어유에틸에스터에서 요소부가법을 이용하여 고도불포화지방산, 특히 eicosapentaenoic acid와 docosahexaenoic acid를 농축할 때 효율에 영향을 끼치는 용매의 종류와 최적 요소량과 효율적인 방법에 대해 살펴보았다. 용매는 5%수화된 아세톤을 사용한 것이 포화지방산과 단일 불포화지방산을 보다 효율적으로 제거하였다. 요소의 양은 시료의 양보다 부가물을 형성하는 포화지방산 및 단일불포화지방산의 양과 밀접한 관계가 있었으며 포화 및 단일불포화지방산양의 5배를 첨가하는 것이 EPA와 DHA를 최대로 농축할 수 있었다. 이 조건하에서 EPA와 DHA는 각각 36%와 41%로 농축되었고 증량수율은 25.1%였다. 복잡한 공정을 단축시키기 위해 요소 column을 통과시켜 실시한 결과 EPA, DHA는 각각 29%, 36%로 농축되어 농축효율은 batch식보다 떨어졌으나 공정이 간단하며 시간이 절약되었다. High yield and efficient enrichment of eicosapentanoic acid(EPA) and docosahexanoic acid(DHA) from fish oil could be obtained by urea adduct formation. The content of EPA and DHA were varied significantly depending on the amount of urea and monoenoic and saturated fatty acids in the sample. Effective organic solvent and the amount of urea are 5% hydrated acetone and 5 times of saturated and monoenoic fatty acid in the sample. With the new method the fraction in which the total content of EPA and DHA is more than 80% could be obtained. Tough the low efficiency of enrichment was showed as 29% of EPA and 36% of DHA by continuous process with urea column but the process was simple and could be saved the operation time.

한약재로부터 대장균 O-157에 대한 항균물질의 검색

김윤진,최원균,조재선 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

한약 처방 76종과 58종의 약재로 각 균주에 대한 항미생물 활성을 디스크 확산법으로 검색한 겨로가 처방에서는 삼소음과 주중황연탕이, 한약재에서는 소목과 황연이 열수와 메탄올 추출물이 모든 균주에 대해 항균 활성이 강한 것으로 검색되었다. 한약처방 76종에서 각 균주에 대해 항균활성이 강한 삼소음과 주증황연탕 추출물의 최소억제농도 실험 결과 삼소음 메탄올 추출물의 최소억제농도는 S. aureus 균주가 0.07 ㎎/㎖로 가장 낮게 나타났고, S. sonnel, S. flexnery, S. typhi는 0.31 ㎎/㎖를 나타냈으며, E. coli O157(V1+V2)와 E. coli O-157(none)에서는 각각 2.5 ㎎/㎖, 1.25 ㎎/㎖로 나타냈다. 주증황연탕 추출물의 최소억제농도는 S. aureus에서 0.01 ㎎/㎖로 가장 낮게 나타났으며, S. sonnei, S. flexnery, S. typhi에서는 각각 0.15 ㎎/㎖, 0.07 ㎎/㎖, 0.31 ㎎/㎖를 보였으며, E. coli O-157(V1+V2)과 E. coli O-157(none)은 5.0 ㎎/㎖를 나타냈다. 장내 세균에 대한 58종의 최소억제농도 실험 결과 이중 가장 높은 항균 활성을 타나낸 것은 소목 추출물로 최소억제농도는 E. coli O157(V1+V2)와 E. coli O157(none)에서 1.25 ㎎/㎖, S. aureus와 S. flexnery에서는 각각 0.39 ㎎/㎖, 0.078 ㎎/㎖를 보였으며, S. sonnei와 S. typhi는 0.15 ㎎/㎖를 나타내었다. This study was conducted for screening of antimicrobial compounds from 76 tradihonal prescriptions and 58 medicmal herbs. Among the prescriptions methanol and hot water extracts of Samsou˘m, Chujunghwang-yon'tang, and Sashinhwan showed antimicrobial activity against E. coli and Chujunghwang-yont'ang showed the highest antimicrobial activity. Among the medicmal herbs, hot water and methanol extracts of Terminalia chebula, Caesalpinia Sappan, and Coptis chiriensis showed the highest antimicrobial activity against E. coli 0157, Staphylococcus nureus, Shigella flexnery, Shigella. sonnet, Salmonella. typhi. Prescription such as Samsou˘m, Chujunghwang-yont'ang, and Sashinhwan were also interesting because both methanol and water extracts were active. Chujunghwang-yont'ang showed the most strong antimicrobial activies for the microoganisms tested. Water and methanol extracts of Terminalla chebuln, Caesalpinia Sappan, and Coptis chmensu showed the highest mhibitory effects on the growth of all bacteria tested.

Application of Vendor-Neutral Iterative Reconstruction Technique to Pediatric Abdominal Computed Tomography

Woo Hyeon Lim,Young Hun Choi,Ji Eun Park,Yeon Jin Cho,이승현,Jung-Eun Cheon,Woo Sun Kim,In-One Kim,Jong Hyo Kim 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.9

ObjectiveTo compare image qualities between vendor-neutral and vendor-specific hybrid iterative reconstruction (IR) techniques for abdominopelvic computed tomography (CT) in young patients. Materials and MethodsIn phantom study, we used an anthropomorphic pediatric phantom, age-equivalent to 5-year-old, and reconstructed CT data using traditional filtered back projection (FBP), vendor-specific and vendor-neutral IR techniques (ClariCT; ClariPI) in various radiation doses. Noise, low-contrast detectability and subjective spatial resolution were compared between FBP, vendor-specific (i.e., iDose1 to 5; Philips Healthcare), and vendor-neutral (i.e., ClariCT1 to 5) IR techniques in phantom. In 43 patients (median, 14 years; age range 1–19 years), noise, contrast-to-noise ratio (CNR), and qualitative image quality scores of abdominopelvic CT were compared between FBP, iDose level 4 (iDose4), and ClariCT level 2 (ClariCT2), which showed most similar image quality to clinically used vendor-specific IR images (i.e., iDose4) in phantom study. Noise, CNR, and qualitative imaging scores were compared using one-way repeated measure analysis of variance. ResultsIn phantom study, ClariCT2 showed noise level similar to iDose4 (14.68–7.66 Hounsfield unit [HU] vs. 14.78–6.99 HU at CT dose index volume range of 0.8–3.8 mGy). Subjective low-contrast detectability and spatial resolution were similar between ClariCT2 and iDose4. In clinical study, ClariCT2 was equivalent to iDose4 for noise (14.26–17.33 vs. 16.01–18.90) and CNR (3.55–5.24 vs. 3.20–4.60) (p > 0.05). For qualitative imaging scores, the overall image quality ([reader 1, reader 2]; 2.74 vs. 2.07, 3.02 vs. 2.28) and noise (2.88 vs. 2.23, 2.93 vs. 2.33) of ClariCT2 were superior to those of FBP (p < 0.05), and not different from those of iDose4 (2.74 vs. 2.72, 3.02 vs. 2.98; 2.88 vs. 2.77, 2.93 vs. 2.86) (p > 0.05). ConclusionVendor-neutral IR technique shows image quality similar to that of clinically used vendor-specific hybrid IR technique for abdominopelvic CT in young patients.

반복적인 무호흡 잠수 시 항산화 반응의 변화

문황운(Moon, Hwang-Woon),최윤진(Choi, Youn-Jin),김정원(Kim, Jeong-Weon),김제원(Kim, Ze-One) 한국체육과학회 2021 한국체육과학회지 Vol.30 No.5

This study is about changes in antioxidant response during repeated apnea diving. The subjects are 16 male university students. The method is to measure MDA, SOD, red blood cell, lactic acid, blood suger, blood and heart rate at rest, after 5, 10, 15 and 20 times of repeated apnea diving in 5M diving pool. One-way ANOVA with repeated measure was performed to verify the difference in the mean between periods, and the LSD method was performed for post-hoc testing if there was any significance between groups. the statistical significance level was set as α=.05. As a result, there were significant differences in white blood cells, red blood cells, hemoglobin, hematocrit, blood glucose, lactic acid, and heart rate changes according to the number of times and time, but there were no changes in MDA and SOD. This showed that the intensity of apnea diving was low in male college students and there was no significant difference due to physical adaptation. Therefore, in future studies, in-depth studies of various variables appearing during apnea diving in various environments and conditions are required.

P024 Increased expression of TRPV3, PAR2 and NK1R in burn scars with post burn pruritus

( Yong Se Cho ),( Yong Won Choi ),( Jee Hee Son ),( Yun Sun Byun ),( Bo Young Chung ),( Hee Jin Cho ),( Chun Wook Park ),( Hye One Kim ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: Post burn pruritus is a common distressing sequela of burn wounds. Empirical antipruritic treatment often fails, as the mechanism has not been fully elucidated. Objectives: The aim of this study was to evaluate the manifestation of transient receptor potential vanilloid 3 (TRPV3), transient receptor potential ankyrin 1 (TRPA1) and other related receptors in post burn pruritus. Methods: Sixty-five burn patients with (n=40) or without (n=25) pruritus were investigated, including skin biopsy and clinical assessments such as VAS (Visual analogue scale) for pruritus, PSAS (Patient scar assessment scale), and OSAS (Observer scar assessment scale). Keratinocytes and fibroblasts from skin biopsy samples were separated and real time PCR, western blotting, intracellular Ca2+ measurements were performed. Results: Real time-PCR showed that mRNA of TRPV3 was significantly increased in keratinocytes from pruritic burn scars than in keratinocytes from non-pruritic burn scars. With TRPV3 activation, intracellular Ca2+ concentrations were more significantly increased in keratinocytes from pruritic burn scars than in those from non-pruritic ones. Additionally, mRNA and protein levels of protease-activated receptor 2 (PAR2) and neurokinin-1 receptor (NK1R) were also significantly increased in pruritic burn scars. Conclusion: We confirmed that TRPV3, PAR2, and NK1R were highly expressed in pruritic burn scars. These results may help determine a novel mechanism for post burn pruritus.

단전이성 뇌종양의 임상 양상과 예후 인자

이재련(Jae Lyun Lee),신창진(Chang Jin Shin),강희정(Hee Jeong Kang),오현아(Hyun Ah Oh),이구(Gu Lee),최재혁(Jae Hyuk Choi),배성화(Sung Hwa Bae),이경희(Kyung Hee Lee),현명수(Myung Soo Hyun),신세원(Sei One Shin),류헌모(Hyun Mo Ryoo) 대한내과학회 2002 대한내과학회지 Vol.62 No.4

목적 : 전이성 뇌종양은 성인 악성 종양환자의 흔한 합병증이다. 저자 등은 전이성 뇌종양의 임상적인 특징, 치료 효과, 생존에 영향을 미치는 예후 인자를 알아보고자 하였다. 대상 및 방법 : 1991년 1월부터 1997년 12월까지 본원에서 전이성 뇌종양으로 진단 받은 97예의 환자를 대상으로 하여 후향적으로 분석하였다. 결과 : 뇌전이의 원발종양으로는 폐암 (61예, 63%)이 가장 흔하였으며 그 외 원발 불명 전이성 뇌암 (15예, 16%), 위장관암 (13예, 13%), 유방암 (6예, 6%), 신장암 (2예, 2%) 순이었다. 단일성 뇌전이는 44예 (45%)였으며 다발성 뇌전이는 53예 (55%)였다. 치료 유무 및 종류를 고려하지 않을 경우 중앙생존기간은 3개월이었으며 1년 생존율은 8%였다. 생존율에 영향을 미치는 유의한 예후인자로는 진단당시 활동능과 신경손상의 정도 (p< 0.01)였다. 어떠한 치료도 시행하지 않는 군에 비해 스테로이드를 포함한 치료를 시행한 군에서 유의한 생존기간의 증가를 관찰할 수 있었다 (1.1개월 대 3.7개월). 스테로이드 단독 치료에 비해 전뇌 방사선 조사를 추가한 군에서 유의하게 생존기간이 연장되었다 (2.2개월 대 4.8개월). 추가적인 항암화학요법은 유의한 생존기간의 향상을 가져오지는 못하였다. 수술적 절제술을 시행한 군의 중앙생존기간은 8.8개월로 스테로이드와 전뇌 방사선치료만 시행한 군에 비해 유의한 생존기간의 증가를 보였다. 결론 : 전이성 뇌종양 환자에서 전뇌 방사선치료와 스테로이드는 유용한 완화치료 방법이며 진단 당시 활동능과 신경손상의 정도가 중요한 예후 인자로 분석되었다. 후향적인 연구로 인해 결과 해석에 한계가 있으나 수술적 절제 및 항암화학요법이 일부 선택된 환자 치료시 중요한 수단이 될 것으로 사료된다. Background : Brain metastasis is a common complication in cancer patients. We evaluated the clinical characteristics, treatment outcome and prognostic factors for patients with metastatic brain tumor. Methods : The records of 97 patients with metastatic brain tumor during the period from January 1991 to November 1997 were reviewed retrospectively. Results : The most common primary tumor is lung cancer (61 cases, 63%) followed by metastatic cancer unknown primary site (15 cases, 16%), gastrointestinal cancer (13 cases, 13%), breast cancer (6 cases, 6%) and renal cancer (2 cases, 2%). There were 44 patients with a single brain metastasis and 53 patients with multiple brain metastases. The median survival was 3.0 months and one-year survival rate was 8% irrespective of treatment. Favorable prognostic factors which affect survival were ambulatory status (p<0.01) and functional neurologic class 1, 2 (p<0.01). Median survival was 3.7 months for patients with steroid therapy and 1.1 months with no therapy (p<0.01). Median survival was 4.8 months for patients with steroid therapy plus whole brain radiotherapy (WBRT) and 2.2 months with steroid therapy alone (p<0.01). Additional chemotherapy did not appear to affect the survival. The patients treated with surgery had median survival time of 8.8 months compared with 2.5 months for patients treated with steroid therapy plus WBRT (p<0.05). Conclusion : In present study, we confirmed that whole brain irradiation and corticosteroid administration are effective palliative treatment for patients with metastatic brain tumor. Initial performance status and neurological function were identified as important prognostic factors. Although confounded by the limitations of retrospective study, more aggressive treatments including surgery and chemotherapy could be regarded to have a significant role to achieve better treatment outcome in some selected cases.(Korean J Med 62:444-452, 2002)