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이희삼,정교순,김선여,류강선,이완주 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1
This study was designed to investigate the blood glucose lowering effect of sericultural products using alloxan-induced hyperglycemic mice. The administration of sericultural products did not affect body weight between normal mice and alloxan induced hyperglycemic mice. High blood glucose level in alloxan-induced mice group was maintained, during all the experimental period. In the 2-3 weeks, a significant decrease in the blood glucose level was observed in the group treated with sericultural products. Up to 5 weeks, blood glucose level of those group was maintained. Sericultural products treatment group have a activity for glucose tolerance test Sericultural products showed the blood glucose lowering activity for loading maltose and sucrose.
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이란산 흑석류 농축액과 그 제품의 성분 및 함유된 Phyto 에스트로겐류에 관한 연구
최원균,정교순,조규성,황명오,유영숙 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.2
천연 호르몬 보충 제제의 개발을 위한 기초 연구로 이란산 흑석류 과즙 농축액과 이를 이용한 제품의 화학성분들을 분석하였다. 석류 농축액의 일반성분은 수분 39.3%, 조지방 0.4%, 조단백질 0.9%, 조회분 1.4%, 그리고 탄수화물은 42.0%이었다. 아미노산 함량은 글루탐산이 1310.Oppm, 아스파르트산이 896.2ppm, 아르기닌이 877.7ppm, 페닐알라닌이 57.5ppm순으필 무기 성분들은 철분 6640.Oppm, 염소 3464.Oppm, 칼륨 2550.8ppm, 인 150.Oppm, 칼슘 80.Oppm 순으로 많이 함유되 어 있었다 비타민은 5가지의 수용성 비타민이 함유되어 있었으며 그 중 비타민 C(20mg/100g)를 제외한 나머지 비타민들이 아주 적은 양이 있었다. 주요 지방산은 palmitic acid(8.3%)와 stearic acid(69.4%)로 전체 지방산의 약 60~80%를 차지하고 있었다. 또한 6종의 phyto및 에스트로겐류가 들어 있었으며 각각daidzein 23.72ppm, quercetin 9,75ppm, catechin 1.48ppm, genistein 0.29ppm, 2,3-di-MeO-estradiol이 0.04ppm, 그리고 17β-에스츠라디올이 0.15ppm이 함유되어 있었다. 이상의 결과들을 석류 농축액으로 제조한 제품 포에버 120과 칡과 대두 isoflavon 농축분말과 비교하다. Phytoestrogens are non-steroidal compounds found in a variety of plants, which exert estrogenic effects in animals. In this study, the physico-chemical properties of Iranian black pomegranate extract and its products as preliminarily research for the developing of natural estrogen supplement were evaluated. The chemical components of Iranian black pomegranate extracts and its product (Forever 120) were analyzed. Proximate compositions of pomegranate extracts were as follows; crude lipid 0.4%, crude protein 0.9%, crude ash 1.4% and carbohydrate 42.0%. Major amino acids of pomegranate extracts are glutamic acid (1310.Oppm), aspartic acid (896.2ppm), arginine (877.7ppm) and phenylalanine (57.5ppm). Fatty acid compositions of pomegranate extract lipid extracted by chloroform-methanol (2:1) were myristic (13.1%), stearic (69.4), oleic acid (6.8%) and palmitic acid (8.3%). Mineral elements were ferrous (6640.Oppm) and potassium (2550.8ppm). Vitamins were composed of ascorbic acid(20.Omg/100g), Vit. B_1 (0.12mg/100g) and niacin (0.80mg/100g), 20 phytoestrogens and 20 estrogens of pomegranate extracts were detected Daidzein (0.29ppm), quercetin (9.75ppm) genistein (0.29ppm) and 17 β-estradiol(0.15ppm). Above the chemical components of pomegranate extracts were compared with that of pome granate its product or other isoflavon concentrates.
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천마(Gastrodia elata Blume)의 항혈소판, 항혈전활성
백영숙,송재경,윤춘희,정교순,윤혜숙,Paik, Young-Sook,Song, Jae-Kyoung,Yoon, Chun-Hee,Chung, Kyo-Soon,YunChoi, Hye-Sook 한국생약학회 1995 생약학회지 Vol.26 No.4
MeOH extract of Gastrodia elata was fractionated to five solvent fractions, hexane fr (fr I), 90% MeOH fr (fr II), EtOAc fr (fr III), BuOH fr (fr IV) and $H_2O$ fr (fr V). Among the five fractions, fr II, III and IV showed platelet anti-aggregating effects against ADP or collagen induced rat platelet aggregation in vitro. Fr II , III and IV were also tested in vivo, in the mouse and rat models of thrombosis. Oral administration of fr II, III or IV enhanced the recovery from the thrombotic shock in the mouse model of thrombosis to 32-40% from 17% of recovery with the control group of mice. Treatment with fr II, III or IV also attenuated the sudden reduction in the blood platelet count following intravenous collagen injection to rat. The above results were indicative of the presence of anti-platelet and anti-thrombotic components in this plant.
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윤혜숙(Hye Sook Yun Choi),정교순(Kyo Soon Chung),김문희(Moon Hee Kim),오재희(Jay Hee Oh) 한국생약학회 1995 생약학회지 Vol.26 No.2
Twentyfive plant crude drugs with traditional reputation were selected for the antithrombotic screening. MeOH (80%) extract of each plant was given (500 ㎎/㎏, p.o.) to mice 1 hr prior to the thrombotic challenge (1.8 ㎎g collagen plus 180 ug epinephrine/Kg, i.v.). Seven extracts (1, 2, 8, 10, 18, 22 and 23) showed either comparable or better recovery than aspirin (50 ㎎/㎏, p.o.) from paralysis caused by the pulmonary thrombosis. 1, 2, 8, 10, 18, 22 and 23 were fractionated to CH₂Cl₂ soluble (fr I) and H₂O soluble (fr II) fractions and each fraction (100 ㎎/㎏, p.o) was also tested. Mice treated with fr I of 2 (Angelica koreana), 10 (Corydalis spp.) or 23 (Schizonepeta tenuifolia) showed higher recovery and lower mortality rates than aspirin (50 ㎎/㎏, p.o) treated mice.
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HPLC 및 GC/MSD를 이용한 식물 중의 Higenamine 함량 분석
윤혜숙,서영배,한영희,송윤선,류재천,정교순,YunChoi, Hye-Sook,Suh, Young-Bae,Hahn, Young-Hee,Song, Yun-Seon,Ryu, Jae-Chun,Chung, Kyo-Soon 한국생약학회 1998 생약학회지 Vol.29 No.2
Higenamine is known as a cardiotonic principle of Aconiti tuber. The analytical procedures were established for the detection of higenamine in plants. The amounts of higenamine in several Aconiti tubers and the embryo of Nelumbo nucifera, another plant species known to contain higenamine, were determined. The $H_2O$ soluble fraction prepared from MeOH extract was first treated with AD-2 resin and then applied to either HPLC or GC/MSD systems. With HPLC, $6.4{\sim}19.2\;{\mu}g/g$ of higenamine were detected from various Aconiti tubers and $182.3\;{\mu}g/g$ of higenamine from the embryo of Nelumbo nucifera. The results obtained with GC/MSD also provided comparable data with those obtained with HPLC.
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