의약품중 잔류유기용매 시험법 확립에 관한 연구

고용석,강찬순,최보경,김혜수,홍정희,최명신,김상현,장성재,김길수 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

Headspace-GC(딘5-GC)를 이용하여 ICH 잔류웅매 가이드라인 class 1, 2에 속하는 28종의 잔류유기웅매 분석방법을 연군한 결과 SPB-5, BB-WAX column을 동시 이용하여 각각을 확인, 정량할 수 있었다.또한 염의 참가효과, 평형시간,평형온도가 headspace분석에 미치는 영향을 검토한 결과 평형 시간 30분,평형온도 85℃,Na₂SO₄Ig을 첨가하여 시험할 때 최적 부넉조건을 나타내었다. 회수율은 1,1-achloroethene(68.3%)를 제외하고 90.9 ~ 114.5%로 패체적으로 양호하였다. 따로 HS-GCJECD, DB-524 column을 이용하여 9종의 잔류유기용매에 대하여 각각의 검랸선을 작성하고 검출한계를 구하였다. 실제 의약품 원료 10종및 제제 5종에 적용시킨 결과 모두 불검출되었으며 본 시험법은 의약품중의 잔류유기용매 분석에 적용할 수 있는 방법이라고 판단된다. The headspace-fC/FID(HS -GC/FID) method was performed for test method developnlent of residu;31 organic solvents in pfrarmaceuticals. Using SPB-S and BB-WAXcolumn, 28 kinds of solvents iri ICH residual sof)·ents guidetine class 1, 2 couBd be individuallt-identified and quantitated. The foIBowing residoal soIYents were not detected by the headspaceinjection condition : N,N-Cmethylacetamide, N,N-dimeth)rlformamide, ethyfeneglycol, formamide,2-methoryethanot, N-rnethylpyrrolidone, suBforane. The effects of the addition of salts,equilibration time, and equilibration temperature on headspace analt·sis were invesugated. Theoptimum conditions weiFe obtained with addtion of Ifa2s04 19 as a salt, simultaneously. thetime and temperature of equitibration were 30min and 8i'f, respectivety. The recovery havefound between 90.9 and 114.5% except 1,1-dichloroethene(fi8.3%). Using DB-624 column &HS-GC/:ECD method, 9 kinds of residual solvents could be individually identified andfuantitated. This HS-GC method can be applied to test the residua3 organic solvent in thepharmaceuticals.

여고 테니스 우수선수와 비우수선수의 호흡순환기능에 관한 연구

고기준,이재규,김종인,이광무,한재웅,신군수,김태운,안병철,문혜경 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to evaluate a study on Cardiorespiratory Functions of Elite Tennis Player and Non-Elite Tennis Player in Female High-School. The subjects were included 2 group(each group n=7); Elite Tennis Player group and Non-Elite Tennis Player group. The conclusion of this study was as follows: 1. Elite Tennis Player group showed significantly high in VO2max(p<.001) 2. Elite Tennis Player group showed significantly high in VCO2(p<.001) 3. Elite Tennis Player group showed significantly high in VO2max/kg(p.05)

청각 이미지와 의복 이미지의 관련성에 대한 탐색 :성격, 성별,의복관심의 영향을 중심으로

배수정;고진아;박소현;이규혜;추호정 한양대학교 2007 韓國 生活 科學 硏究 Vol.27 No.1

This study exploratory examined the relationship between auditory images and clothing style images of young consumers by their personality types, sex and clothing interest. A questionnaire was developed to measure auditory images, clothing style images, personality types, clothing interest and demographic variables. Scale items were adopted from prior studies on clothing self images and Myers-Briggs Type Indicator (MBTI) known to measure psychological types. 120 undergraduate students with diverse majors were the respondents of the empirical study. MBTI categorized respondents into one of 3 personality types, IFT (Introversion and Thinking or Feeling, n=23), EF (Extroversion and Feeling, n=73) and ET (Extroversion and Thinking, n=24). ANOVA results indicated that, among 13 auditory images measured in the study, rational, self-confident and social images were significantly different across three personality type groups. Overall, ET group showed higher level of the the auditory images. Among clothing style images, social images were significantly different. IET group showed significantly lower level of social images. In addition, respodents' age and level of clothing interest influenced auditory and clothing style images. Correlation coefficients indicated that there were significant relationships between most auditory and clothing style images included in the study except for the self-confident image.

주의산만을 주소로 소아정신과를 내원한 아동의 인지적 특성 : T.O.V.A. 양상을 중심으로

신의진,이수진,이혜란,고려원 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.2

본 연구에서는 주의산만을 주소로 내원하는 아동을 진단 준거에 따라 분류하고 각 진단 집단을 설명할 수 있는 인지적 특성을 T.O.V.A. 양상을 중심으로 설명하였다. 소아정신과를 방문한 65명의 아동을 각각 주의력결핍 과잉행동장애(38명), 틱장애(17명), 그리고 기타 정서장애(10명) 집단으로 분류한 후 주의산만의 정도에 영향을 미칠 수 있는 집단의 연령 및 지능(전체 지능, 동작성 지능, 언어성 지능)을 먼저 파악한 후 T.O.V.A.의 중다구인(누락오류, 오경보오류, 정반응시간, 반응시간 표준편차, 예기반응, 다중반응)을 통해 주의산만의 양상을 살펴보았다. 세 집단 간에 연령에서 집단 간에 유의미한 차이가 나타나 연령 수준의 차이에서 기인한 효과를 배제한 결과, 언어성 지능에서 세 집단 간에 유의미한 차이가 나타났다. 또한, T.O.V.A.의 정반응 시간(전반부, 후반부, 전체)에서 세 집단간에 유의미한 차이가 있는 것으로 나타났다. 주의력결핍 과잉행동장애 집단은 틱장애 및 기타 정서장애 집단보다 T.O.V.A.의 정반응 시간이 길어 정보처리의 지연을 시사해 주었다. 한편 주의산만을 주소로 내원하는 아동의 경우 T.O.V.A. 양상과 더불어 연령과 지능의 효과에 대해 신중히 고려해야 할 필요가 있겠다. 중심 단어 : 인지적 특성ㆍ주의산만ㆍT.O.V.A.ㆍ정반응시간ㆍ주의력결핍 과잉행동장애ㆍ틱장애ㆍ기타 정서장애. Objective : This study aims to investigate the cognitive characteristics of clinically referred children with symptoms of inattention, cash as having ADHD, tic disorder, and emotional disorder. Methods : 65 boys(38 with ADHD, 17 with Tic disorder, and 10 with Emotional disorder) were individually assessed using the KEDI-WISC(FIQ, VIQ, PIQ) and T.O.V.A.(errors of omission, errors of commission, reaction time, variability, anticipatory response, multiple response), and the results of those tests were analyzed. Results : There was significant difference among three diagnostic groups of the VIQ of KEDI-WISC and the reaction time of T.O.V.A. after the correction of the effect of age difference. Conclusion : The finding suggest that the reaction time of T.O.V.A. might be the useful variable to differentiate the ADHD from other psychiatric disorders and the effect of age and IQ difference should be considered carefully to diagnose in clinical setting. KEY WORDS : Cognitive characteristicㆍInattentionㆍT.O.V.A.ㆍReaction time.

Three cases of lymphatic malformations treated with sono-guided sclerotherapy using bleomycin

( Hye Soo Ko ),( Si Hyub Lee ),( Seung Dohn Yeom ),( Jong Hyuk Moon ),( Jeonghyun Shin ),( Gwang Seong Choi ),( Ji Won Byun ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Lymphatic malformations(LMs) are benign cystic masses resulting from the abnormal development of lymphatic channels. Historically, surgical excision has been the first-line therapy for LMs. However, this treatment shows high rates of recurrence and the risk of complications such as scar. Recently, sclerotherapy has been reported as promising alternative treatment for LMs. We experienced three cases of LMs who were treated with bleomycin sclerothreapy. Three patients presented with grouped fluid filled papules from early childhood. The anatomic sites of the patients were right flank, left shoulder and left buttock. Two of them had a history of surgical excision with recurrence. They were diagnosed as LMs and treated with single or two sessions of sono-guided sclerotherapy using bleomycin. In all 3 cases, the lesions were showed significant improvement without complication. In conclusion, we suggest that sono-guided sclerotherapy using bleomycin is a safe and effective treatment option for LMs and this is the first case report on LMs treated with bleomycin sclerotherapy in Korean dermatologic literature.

Lip psoriasis

( Hye Soo Ko ),( Si Hyub Lee ),( Seung Dohn Yeom ),( Jong Hyuk Moon ),( Ji Won Byun ),( Gwang Seong Choi ),( Jeonghyun Shin ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

The predilection sites of psoriasis are trunk, extremities, scalp and nails. The disease may occasionally involve the anus and genitalia, but lip involvement is very rare. Although cutaneous findings are generally sufficiently distinct to make a diagnosis, diagnosis can be difficult in cases of unusual involvement. Herein, we report a case of 27-year-old man with a history of fissuring and cracking of his lips for about 4 years. He had no skin lesions except the lip lesions. Histopathological examination showed psoriasiform hyperplasia with diminished granular layer, parakeratosis with collection of neutrophils and dilated tortuous vessels in the papillary dermis. We believe that this case emphasizes that psoriasis should be included in the differential diagnosis in chronic and relapsing eczema like eruption of lips and this is the first case of psoriasis of the lips in Korean dermatologic literature.

A case of morpheaform sarcoidosis

( Hye Soo Ko ),( Si Hyub Lee ),( Seung Dohn Yeom ),( Jong Hyuk Moon ),( Ji Won Byun ),( Gwang Seong Choi ),( Jeonghyun Shin ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Sacoidosis is a multisystem disease which is characterized by the presence of non-caseating granulomas in the affected organs. Cutaneous lesions occur in 20%~35% of patients with systemic sarcoidosis. Nonspecific cutaneous lesions do not contain granuloma formation histologically and regarded as a reactive process. Specific cutaneous lesions are characterized by non-caseating granulomas and include papules, plaques, nodules, verrucous, atrophic, pustular, psoriasiform, ulcerative, erythrodermic and ichthyosiform eruptions. Herein, we report a case of 71-year-old woman who was clinically resembling morphea but was diagnosed as sarcoidosis with histopathologic examination showing subcutaneous sarcoidal granuloma with changes of morphea.

A case of herpes syringitis

( Hye Soo Ko ),( Si Hyub Lee ),( Seung Dohn Yeom ),( Jong Hyuk Moon ),( Ji Won Byun ),( Gwang Seong Choi ),( Jeonghyun Shin ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Herpes simplex virus (HSV) is a member of the herpes virus family which commonly affects the skin and the histopathologic changes are often limited to the epidermis. Although the virus may occasionally involve the follicular epithelia (herpetic folliculitis), the involvement of the sebaceous and eccrine structures (herpetic syringitis) is very rare. Herpetic folliculitis and syringitis can be diagnosed by histopathologic examination and confirmed by detection of HSV DNA or HSV-specific antibodies. A 70-year-old woman presented with asymptomaticerythematous crusted plaques on the left cheek for 3 months. She has been taking systemic steroids, methotrexate and tacrolimus for 6 years due to rheumatoid arthritis. Histopathologically, the eccrine glnad ductal cells showed ballooning degeneration and molding of nuclei and were positive on immunohistochemical staining for HSV. Cutaneous lesions were improved with antiviral agent for 2 weeks. This is the first case report on herpes syringitis in Korean dermatologic literature

Folliscope analysis of temporal involvement in Korean women with female-pattern hair loss

( Hye Soo Ko ),( Si Hyub Lee ),( Seung Dohn Yeom ),( Jong Hyuk Moon ),( Ji Won Byun ),( Jeonghyun Shin ),( Gwang Seong Choi ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: FPHL affects the central portion of the scalp. In some patients, the temporal area of the scalp also can be involved. But,there are few pre-existing classification systems that evaluate this area. Objectives: To evaluate the degree of temporal involvement in Korean women with FPHL Methods: Total of 27 Korean women with FPHL wereenrolled. All subjects were classified according to the BASP classification. We evaluated the temporal involvement and added 1 more specific type T to BASP classification(T1:mild,T2:moderate,T3:severe). Hair density and thickness were measured using folliscope. 4 scalp sites including front,vertex,back and temple were targeted. Results: Forty eight percent of patients with FPHL showed temporal involvement. Among 27 subjects,12 and 1 subjects were classified as T1 and T2 each. There was statistically significant correlation between T grade and temporal hair density(p=0.042). The range of temporal hair density was 105.78【29.66/Р. The temporal/occipital ratio(T/O) of the total hair density was 0.72【0.16. TheT/O and frontal/occipital ratio(F/O) of the total hair density was positively correlated(Spearman correlation r=0.502,p=0.008). The T/O and F/O of the thick hair(>80Л) density was not correlated(Spearman correlation r=0.057,p=0.778). Conclusion: The results in this study suggest that there is decrease in the temporal hair density compared with the occipital hair density in some patients with FPHL. We suggest adding specific type T to BASP classification.

Development of a Test Method for the Evaluation of DNA Damage in Mouse Spermatogonial Stem Cells

Hye Lyun Jeon,Jung-Sun Yi,Tae Sung Kim,Youkyung Oh,Hye Jeong Lee,Minseong Lee,Jin Seok Bang,Kinarm Ko,Il Young Ahn,Kyungyuk Ko,Joohwan Kim,Hye-Kyung Park,Jong Kwon Lee,Soo Jung Sohn 한국독성학회 2017 Toxicological Research Vol.33 No.2

Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro comet assay in our previous study. This study aimed to verify the validity of in vitro tests involving mSSCs by comparing their results with those of in vivo tests using C57BL/6 mice by gavage. We selected hydroxyurea (HU), which is known to chemically induce male reproductive toxicity. The 50% inhibitory concentration (IC50) value of HU was 0.9 mM, as determined by the MTT assay. In the in vitro comet assay, % tail DNA and Olive tail moment (OTM) after HU administration increased significantly, compared to the control. Annexin V, PI staining and TUNEL assays showed that HU caused apoptosis in mSSCs. In order to compare in vitro tests with in vivo tests, the same substances were administered to male C57BL/6 mice. Reproductive toxicity was observed at 25, 50, 100, and 200 mg/kg/day as measured by clinical measures of reduction in sperm motility and testicular weight. The comet assay, DCFH-DA assay, H&E staining, and TUNEL assay were also performed. The results of the test with C57BL/6 mice were similar to those with mSSCs for HU treatment. Finally, linear regression analysis showed a strong positive correlation between results of in vitro tests and those of in vivo. In conclusion, the present study is the first to demonstrate the effect of HU-induced DNA damage, ROS formation, and apoptosis in mSSCs. Further, the results of the current study suggest that mSSCs could be a useful model to predict male reproductive toxicity.