세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

제대혈 조혈모세포 분리를 위한 적혈구 제거 방법에 관한 연구

서원석,김숙자,정희정,박경배,박성규,원종호,홍대식,박희숙,신상만 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 :현재까지 제대혈 은행에서 적혈구 제거후에 냉동 보관 방법이 보편화되지 못한 것은 Ficoll, lysis, starch와 중력에 의한 방법 등이 약 50-90%에 이르는 조혈모세포가 손실된다는 이유 때문이다, 따라서 대부분의 제대혈 은행에서 전체 제대혈을 냉동보관 후에 이식에 사용하고 있다. 지금까지의 보고들은 서로 다른 제대혈에서 여러 가지 방법으로 적혈구제거를 실시한 것으로 결과가 서로 큰 차이가 난다. 방법 : 저자는 같은 제대혈에서 Ficoll, gelatin, methylcellulose, RBC lysis 등의 방법으로 적혈구를 제거하여 다음과 같은 결과를 얻었다. 결과: 1) 채취한 평균 제대혈량은 105±23ml,단핵구수는 18.6±4.09×10^(6)/ml이었다. 2) 각각의 방법으로 적혈구를 제거한 다음의 단핵구수는 Ficoll, 방법 4.43±1.50×10^(6)/ml, gelatin방법 7.71±0.37×10^(6)/ml, methylcellulose 방법 5.90±0.51×10^(6)/ml, RBC lysis방법10.72±2.74×10^(6)/ml개 였으며 이 중 RBC lysis방법에서 단핵구 수가 가장 많았다. 3) 각각의 방법으로 적혈구를 제거한 다음 flow cytometry로 측정한 CD34 양성세포 수는 Ficoll 방법 2.65±0.52×10⁴/ml, gelatin방법 3.50±0.43×10⁴/ml, methylcellulose방법 2.71±0.23×10⁴/ml, RBC lysis방법 9.36±1.62×10⁴/ml개 였으며 이 중 RBC lysis방법에서 CD34양성세포 수가 가장 많았다. 전체 세포에 대한 CD34양성세포의 비율을 보면 Ficoll방법 0.72±0.14, gelatin 방법 0.50±0.08%, methylcellulose 방법 0.44±0.03%, RBC lysis방법 0.79±0.04%였다. 4) CFC수는 Ficoll 방법 21.73±11.77×10³/ml, methylcellulose 방법 15.33±7.14×10³/ml, RBC lysis 방법 26.50±4.26×10³/ml개 였으며 이 중 RBC lysis 방법에서 CFC수가 가장 많았다. 결론 : 이상의결과로 보나 Ficoll, nethylcellulose, gelatin, RBC lysis 등의 방법을 이용하는 경우 모두 적혈구 제가가 잘 되었으며 특히 RBC lysis방법을 이용한 경우 가장 많은 CD34양성세포 수와 CFC수를 얻어 적혈구 제거 방법으로 가장 좋았다. 하지만 다른 보고들에서는 gelatin에 의한 방법이 좋다고 하였는데 이는 실험 방법의 차이에 의한 것으로 보여 이에 대한 연구가 좀 더 많은 례에서 이루어져야 할 것으로 사료된다. Background : Human umbilical cord blood (UCB) has been successfully used as an althemative source of allogeneic hematopoietic stem cells for pediatric transplantation. A bank of cryopreserved UCB has the advantage over existing volunteer marrow donor registries because time consuming donor tracing can be avoided. Efficient clinical banking of UCB requires volume reduction of cord blood collections to decrease storage space and costs. The simplest way to achieve this is no deplete cord blood red cell before cryopreservation. Methods : We compared four different red cell depletion techniques, Ficoll, gelation, methylcelulose and red cell lysis method in terms of mononuclear cell count, CD34-positive cell count and colony forming cell (CFC) count. Results : 1) Mean volume and mononuclear cell count of collected UCB were 105±23mL and 18.63±4.09×10^(6)/mL. 2) Among different red cell depletion techniques, red cell lysis mothod showed the highest yeild of mononuclear cell counts, CD34-positive cell counts and CFC counts. Conclusion : Cord blood collection processed with red cell lysis could provide enough stem cells for engraftment but other studies revealed different result. This may be due to the different study method. We concluded that RBC lysis method is effective for the red cell depletion of cord blood without major losses of CFC or CD34-positive cells.

당뇨병 환자에 있어서 소양증에 대한 임상적 관찰 및 Hydrometer와 Corneometer를 이용한 피부 수분량의 측정

서대헌,은희철,신찬수,윤재일,박석범,김상덕,윤춘식 대한피부과학회 1999 대한피부과학회지 Vol.37 No.3

Background: Diabetes mellitus is a common condition, and the majority of patients have one or more cutaneous complications. Among them, xerosis is well known to be closely related to diabetics. The xerosis is inversely reflected upon skin surface hydration, the state of which can be exactly assessed with various investigating instruments, such as a hydrometer and corneometer. Objective: Our purpose was to compare the hydration state of diabetics with normal persons by the use of a corneometer and hydrometer. Methods: A total of 267 diabetics were asked whether they felt xerosis or pruritus. In a subgroup of 31 patients, the water content was evaluated and compared by the parameters of diabetic severity. Forty-one non-diabetics were selected for the normal control. Results : There was a significant difference in water contents between diabetics and non-diabetics. In diabetics, patients with shin spots and altered renal function showed lower values, revealing decreased water contents. Other parameters, including age of patients duration of diseases, status of microangiopathic complications, insulin dosage, HbAlc and c-peptide, were not related to the water contents. Conclusion ; This data indicates that xerosis is closely associated with diabetics and related to clinical and laboratory fidings revealing the disease severity. The hydrometer and corneometer are recommended for the use of the evaluaton and management of xerosis.

Production and Rheological Properties of Bioflocculant Produced by Bacillus sp. DP-152

( DAE OOK KANG ),( HEE MOCK OH ),( Hyun Hyo Suh ),( Byung Dae Yoon ),( HEE SIK KIM ),( SEONG HOON MOON ),( Hyoung Kab Kim ),( Gee Ill Jun ),( Hyun Seoun Park ) 한국미생물생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.6

EPILEPSY AND NEURONAL CELL DEATH IN PHOSPHOLIPASE C β-1 MUTANT MICE

(Dae Soo Kim),(Seong Beom Lee),(Nae Gyu Kang),(Do Sik Min),(Young Hoon Kim),(Sung Ho Ryu),(Pann Ghill Suh),(Hee Sup Shin) 생화학분자생물학회 1997 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

장기적 트립토판 결여식이 생쥐의 유해감각예민도에 미치는 영향

서영대,박제민,김명정,전명호,양구범,장세헌,강철중,김정태,김용식 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

The depressive patients can be divided into two subgroups by presence and absence of pain symptoms. Those without pain symptoms have blunted pain sensitivity whereas those with pain have normal range of pain sensitivity, and are clinically characterized by anxiety and irritability. The aim of the study is to test if these clinical profiles of the depressives with pain symptoms are related with reduced level of brain serotonin(5-HT). Forty four mice were randomly divided into two groups : one group to be bred with tryptophan free diet and the other with normal control diet, each for 4 weeks. Before the beginning of the breeding period, measured were locomotor activity by open field test, anxiety by elevated plus maze and nociceptive sensitivity by tail flick test, before and after forced swimming(FS). During each FS, duration of immobilization was also measured. The sable sets of measurements were repeated at the end of the breeding period. The brain 5-HT and 5-hydroxyindoleacetic acid(5-HIAA) were measured by high performance liquid chromatography. The results were as follows 1.Four weeks of tryptophan free diet reduced significantly body weight, brain weight, and levels of 5-HT and 5-HIAA in the whole brain. 2.Tryptophan depletion did not influence basal nociceptive sensitivity as measured by tail flick latency before FS. The normal blunting of the pain sensitivity induced by forced swimming was preserved in the tryptophan depleted group. 3.Tryptophan depletion did not influence general locomotor activity in open field. 4.Tryptophan depletion increased significantly time spent on the open arms at the elevated plus maze test done before FS. This anxiolytic-like effect was reduced by FS. 5.Tryptophan depletion did not influence duration of immobilization during FS. From these results, it is suggested that the genesis of the depression with pain symptoms is not medicated by quantitative reduction of brain serotonin

A Lipopeptide Biosurfactant Produced by Bacillus subtilis C9 Selected through the Oil Film - collapsing Assay

HEE - SIK KIM,CHANG - HO LEE,HYUN - HYO SUH,KEUG - HYUN AHN,HEE - MOCK OH,GI - SEOK KWON,JI - WON YANG,BYUNG - DAE YOON 한국미생물생명공학회 1997 Journal of microbiology and biotechnology Vol.7 No.3

모바일 그리드에서 체크포인트 기반 작업 이주 기법

정대용 ( Dae Yong Jung ),서태원 ( Tae Weon Suh ),정광식 ( Kwang Sik Chung ),유헌창 ( Heon Chang Yu ) 한국컴퓨터교육학회 2009 컴퓨터교육학회 논문지 Vol.12 No.4

어도내 유속에 따른 어류체형비 비교

배대열 ( Dae-yeul Bae ),문운기 ( Woon-ki Moon ),김도현 ( Do-hyun Kim ),이의행 ( Eui-haeng Lee ),김원장 ( Won-jang Kim ),서정빈 ( Jung-bin Suh ),안중식 ( Joong-sik Ahn ),김재구 ( Jai-ku Kim ),안광국 ( Kwang-guk An ) 한국환경생태학회 2016 한국환경생태학회 학술대회지 Vol.2016 No.2

어류의 외부 형태적 특징은 어류의 유영력과 군집의 기능을 결정하는 중요한 요인이 되고 있다. 어류의 외형은 크게 6개 타입으로 나누어진다. 유선형(streamline)과 같은 방추형(fusiform), 그 외 체고(body depth)와 체폭(body width)에 따라 측편형(compressiform), 종편형(depressiform), 장어형(anguilliform), 리본형(taeniform), 그리고 구형(globiform)등으로 구분된다(Kim and Park, 2002). 어류의 유영성은 유선형에 가까울수록 부피에 대한 지체계수(drag coefficient)는 최소가 됨으로서 최대가 된다(Jacobs et al., 1933; Hoerner, 1965). 어류의 유선형 정도를 측정하는 방법은 체장(body length)/체고(body depth)의 비로 표현되는 어류 체형비(fineness ratio, FR)가 있다. 어류의 유영 및 이동에서 최적 FR비는 4.5이상에서 지체계수(drag coefficient)가 최소인 것으로 알려져 있다(Scarnecchia, 1988). 일반적으로 유영속도는 체장에 비례하며 순항속도(cruising speed), 유지속도(sustaining speed), 그리고 돌진속도(darting speed)로 구분된다(Nakamura, Shunroku, 1991). 이때 어류의 유영력은 유영속도에 유속을 더한 값으로 정의된다(김진홍). 어종간에 유영속도가 일정하다고 가정하면 어류의 유영력은 유속에 좌우되고, 하천에서 어도구조물 설계시 어종들의 한계유속은 어류가 소상하기 위한 지표가 될 수 있다. 특히 어도의 유량 및 유속은 어도를 이용 할 수 있는 개체크기를 제한하는 요인이 되기도 한다. 하천에서 어류 군집의 특성은 서식지내 유속환경을 이해하는데 중요한 요인이 된다. 여울과 소가 연속적으로 나타나는 하천에서 어류 군집은 서식지복잡성(habitat complexity), 미소서식지(microhabitat)의 발달에 따라 다양한 어류가 출현하게 되고, 출현하는 어류의 외형을 분석하면 하천의 흐름의 특성과 밀접한 관련성을 가진다. 유속이 빠른 여울과 소(pool)나 정수역(rentic water)에 서식하는 어종의 FR값은 서로 차이를 보이게 된다. Langerhans and Reznick(2011)의 연구에 따르면 Poecilliidae에 속하는 50여종의 어류를 대상으로 조사한 결과 유속이 빠른 환경에 서식하는 어류는 유선형에 더 가깝고 FR은 4.5에 근접하는 것으로 보고하고 있다. 이는 유속이 어류의 외부 형태적 진화에 중요한 역할을 하는 것임을 의미 한다. 어도를 이용하는 어류군집의 특성을 고려한 설계가 필요하다. 즉 군집의 어류 체형비를 고려하여 어도의 유속분포를 결정하는 국내어도 설계상 가장 큰 문제점은 하천에 서식하는 어류군집의 생태적 특성을 고려하지 않고 일률적으로 어도를 설계하는데 문제가 있다. 우리나라는 갈수기와 홍수기의 유량에 따라 어도내 유속의 차이가 매우 크게 나타난다. 따라서 본 연구에서는 하천의 인공구조물인 어도에서 유속분포에 따라 이를 이용하는 어류들의 형태학적 체형비를 분석함으로서 향후 어도 설계시 어류상 및 목표어종에 따라 어도내 유속 분포를 결정하는데 유용한 자료로 활용을 목적으로 한다. 국내에 서식하는 어류를 대상으로 어도에서 유속의 범위에 따른 어류 체형비 분석을 통해 하천구조물인 어도에서 유속의 특성에 따른 어류체형비를 비교분석하였다. 향후 어도 설계시 어류상 및 목표어종에 따라 유속 분포를 결정하는데 중요한 자료가 될 것으로 판단한다.

IgA 腎病症에 對한 病理學的 및 免疫病理學的 硏究

이종대,서광선,이충식 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

A clinicopathologic analysis for 88 cases of IgA nephropathy has been carried cut from a series of 333 renal biopsy specimens which were studied at Department of Pathology, College of Medicine, Chungnam National University, during a period of 54 months from October 1983 to March 1988. The results were as follows: 1. IgA nephropathy was 26.4% of renal biopsy specimens and 30.4% of primary glomerulonephritis. It was common in young adults and 77.3% of patients were between 16 years and 39 years. Male to female ratio was 1.1 : 1. 2. Modified classification of Meadow et al. for Henoch-Schoenlein nephritis was adopted for the histologic grading of glomerular lesions. The result was as follows; Grade I 5.7%, Ⅱ 46.6%, M 45.5%, Ⅳ 1.1%, an V 1.1%. The clinical class was possible in 70 cases; Class B38.6 %, C 61.4%. 97.1% of these. 70 cases were within the histologic Grade Ⅲ. 3. The results of immunofluorescent study were as follows; All cases showed granular mesangial deposits of IgA. Complement 3 was found, in same manner as IgA, in 83 0%, fibrinogen 23.9%, IgM 18.23%, and IgG 3.4%. 10.2% of these cases showed clumps of fluorescent deposits involving the mesangium and continuous loops. 4. Among the chief complaints on admission, gross hematuria was observed in 59.1%, microscopic hematruia 39.8%, and proteinuria 69.3%, including nephrotic range protein uria 6.8%. Along the histologic grading, the frequency of gross hematuria was different, as follows; Grade I 100.0%, Grade Ⅱ 65.9%, and Grade Ⅲ 47.5%. Statistically, these differences were significant(P<0.01) On serologic study, hepatitis B surface antigen was positive in 6.7% and level of serum IgA was significantly increased in 27.6%.