Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

졸-겔법으로 합성된 폴리이미드/실리카 복합체의 환경적 물성에 관한 연구

박성수,홍성수,이성환,김성완,박재현,김지경,박희찬 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Two types of polyimide/silica composite films were prepared using sol-gel method through hydrolysis and polycondensation of tetraethoxsilane(TEOS) with the polyamic acid(PAA) and end-capped PAA solution. Sample were characterized by the means of differential thermogravimetry, X-ray diffractometry, scanning electron microscopy, universal test, impedance analyzer, chemical resistance test, etc. All of the PAA/silanol solutions heat-treated at 300℃ for 6h were transformed to polyimide/silica composites. It has been demonstrated that the properties of polyimide/silica composites were affected by the amount of silica addition and the bond type existed between polyimide and silica.

파킨슨씨병 환자에 있어서 자가부신수질 이식술

정상섭,박상근,오성훈,김선호,윤도흠,박정수,양우익,최인준,안영수,김경환,박창일,김진수,이현철 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.6

Recent experimental studies and clinical cases have suggested that grafting tissue from the adrenal medulla into the brain may alleviate the symptoms of Parkinson's disease. Autologous adrenal medullary grafts into the striatum was performed stereotactically in two patients with Parkinson's disease. Both patients were bedridden preoperatively, but 4 months after the surgery both became ambulatory and one was even capable of returning to his job. Even though the number of cases was small⑵ and the follow-up period was short, this study shows that adrenal medullary transplantation into the striatum may have some beneficial effects in patient with Parkinson's disease. Further experimental and clinical trials are necessary to see whether this procedure will be a recommendable surgical treatment for patients suffering from Parkinson's disease.

Correlations between the Growth Period and Fresh Weight of Seed Sprouts and Pixel Counts of Leaf Area

( Daesik Son ),( Soo Hyun Park ),( Soo Chung ),( Eun Seong Jeong ),( Seong Min Park1 ),( Myong Kyoon Yang ),( Hyun Seung Hwang ),( Seong In Cho ) 한국농업기계학회 2014 바이오시스템공학 Vol.39 No.4

Purpose: This study was carried out to predict the growth period and fresh weight of sprouts grown in a cultivator designed to grow sprouts under optimal conditions. Methods: The temperature, light intensity, and amount of irrigation were controlled, and images of seed sprouts were acquired to predict the days of growth and weight from pixel counts of leaf area. Broccoli, clover, and radish sprouts were selected, and each sprout was cultivated in a 90-mm-diameter Petri dish under the same cultivating conditions. An image of each sprout was taken every 24 hours from the 4th day, and the whole cultivating period was 6 days, including 3 days in the dark. Images were processed by histogram inspection, binary images, image erosion, image dilation, and the overlay image process. The RGB range and ratio of leaves were adjusted to calculate the pixel counts for leaf area. Results: The correlation coefficients between the pixel count of leaf area and the growth period of sprouts were 0.91, 0.98, and 0.97 for broccoli, clover, and radish, respectively. Further, the correlation coefficients between the pixel count of leaf area and fresh weight were 0.90 for broccoli, 0.87 for clover, and 0.95 for radish. Conclusions: On the basis of these results, we suggest that the simple image acquisition system and processing algorithm can feasibly estimate the growth period and fresh weight of seed sprouts.

NSIP와 BOOP의 고해상전산화단층촬영 소견과 비교

백상현,박재성,차장규,홍현숙,김대호,박성진,조준희,이혜경,최진수 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose: BOOP and NSIP also share similar clinical features such as subacute flu-like illness, the finding of bronchoalveolar lavage, the result of pulmonary function test and more favourable outcome than usual interstitial pneumonia(UIP). The author try to compare the HRCT findings of NSIP with BOOP by pattern and distribution of diseases. Materials and Methods: HRCT findings of histopathologically proven NSIP(Group Ⅰ; 6, Group Ⅱ; 7, Group Ⅲ; 2) in 15 patients(5 male, 10 female, age range, 39-69 years; mean age, 53 years), BOOP in 15 patients(6 male, 9 female, age range, 26-76 years; mean age, 52 years) were reviewed retrospectively. Results: The common pattern of the two diseases was admixed consolidation, ground glass attenuation & reticular densities with/without architectural distortion(NSIP; 53.3%, BOOP; 46.7%). The main component of the pattern wan predominantly consolidation(NSIP; 46.7%, BOOP; 73.3%) and ground glass attenuation(NSIP; 40.0%, BOOP;20.0%). Architectural distotion was more provinent finding with NSIP(mild ; 3, moderate ; 4, severe; 2) compared with BOOP(mild; 7, moderate; 2, severe; 0). The predominant overall distribution was lower lung, peribronchovascular & peripheral lung field in both of the two entities(NSIP;73.3%, BOOP; 73.3%). Peribronchovascular involvement was more common with NSIP(100%) than BOOP(80%), whereas peripheral involvement was more common with BOOP(93.3%) than NSIP(80%). Conclusion: The HRCT findings of NSIP and BOOP were similar in pattern and distribution. But NIP manifested more frequently ground-glass attenuation, whereas BOOP showed more frequently consolidation. Architectural distortion was more severe with NSIP than BOOP. NSIP invoved more frequently peribronchovascular space and BOOP were more common in peripheral lung field.

폐결핵 환자 혈청에서 수용성 Interleukin 2 수용체 및 Adenosine Deaminase 활성도에 관한 연구

고정희,박성규,백상현,박찬권,박병수,안진영,최우석,박정규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Interleukin-2 (IL-2) induces T cell proliferation in an autocrine manner and provides a means by which antigen triggered T cells can be clonally expanded in vitro. During the following activation, the activity of IL-2 is mediated by specific high affinity IL-2 binding membrane receptors which are expressed shortly after activation. In this process, a 42 KD-fragment (soluble IL-2R) is continuously cleaved off and circulates as a soluble marker of T lymphocyte activation. Elevated level of soluble IL-2R has been identified in the serum of patients with malignant autoimmune and allergic disorders, systemic parasitic infection, undergoing graft versus host disease, acute or chronic lymphocytic leukemia and HIV-infection. ADA (adenosine deaminase) completes the process of differentiation of T cell and is essential for progression of T cell maturation. Therefore level of ADA is to correlated with magnitude of T cell immune response. The fact that expression of sIL-2R and ADA activity increases in peripheral blood T-lymphocytes of patients with active pulmonary tuberculosis suggests that T cell activation might have a major role in the pathogenesis of pulmonary tuberculosis. In order to evaluate the T cell immune response in pulmonary tuberculosis, we measured the serum concentration of sIL-2R and ADA activity in 17 patients with current pulmonary tuberculosis, 10 chronic inactive pulmonary tuberculosis, 10 as normal controls. (1) Current pulmonary tuberculosis had significantly higher levels of sIL-2R (237.24±95.47)when compared with those of inactive tuberculosis (78.6±11.06). and the control (68.17±15.4) group. (2) ADA activity in current pulmonary tuberculosis was significantly increased (34.41±20.63) when compared with those of inactive tuberculosis (24.7±14.36) and control (17.65± 5.94) group. (3) There was good correlation between sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group. (4) sIL-2R concentration and ADA activity was decreased significantly 6 months after anti-tuberculosis drug medication. In conclusion, sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group was increased when compared with those of inactive pulmonary tuberculosis and the control group. By the way, T cell mediated immune response was enhanced in current pulmonary tuberculosis, but in inactive chronic pulmonary tuberculosis who had treated by antituberculosis drug medication, the concentration of sIL-2R and ADA activity was nearly normal.

후복막섬유증 5례의 임상적 고찰

정충식,김수현,안병수,심대성,박도영,김철성,장대수 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Retroperitoneal fibrosis is a fibrotic process of the retroperitoneum that frequently produces ureteral obstruction. It can occur as an idiopathic disease or in association with various non-malignant and malignant condition. In the early phase of the retroperitoneal fibrosis ; symptoms originate from the disease process itself, while in the late state the clinical features represent the effect of obstructive uropathy and renal failure. Treatment consist of steroid therapy, urinary diversion and ureterolysis with jntraperitonealization or lateralization or wrapping with omentum. Herein we present 5 cases of retroperitoneal fibrosis.

지상라이다를 이용한 미기록 외래종 갯쥐꼬리풀(Spartina Alterniflora)의 분포특성과 관리방안 연구 -다도해 해상국립공원 진도 남동리 해안을 사례로-

박정원 ( Jung Won Park ),김하송 ( Ha Song Kim ),장성건 ( Seong Geon Jang ),천숙진 ( Sook Jin Chun ),육관수 ( Kwan Soo Yuk ) 한국도서학회 2015 韓國島嶼硏究 Vol.27 No.3

갯쥐꼬리풀(Spartina alterniflora)은 북미 대서양 연안 뉴펀들랜드, 캐나다 남쪽에서 아르헨티나 북부의 갯벌이 원산이며, 생장이 강한 특성으로 세계의 기수지역과 해안에서 생태공학적 용도로 도입된 바 있다. 그러나 매우 빠르게 갯벌을 피복하여 자생종의 생장을 방해하고, 염습지 종다양성을 감소 시켰고, 결과적으로 과다생육으로 갯벌 무척추동물의 서식지를 잠식해 사라지게 하여 무척추동물을 먹이로 하는 조류 또한 생태계 내에서 감소키는 등 많은 환경문제를 야기 시켰다. 우리나라에서는 2008년 전후에 다도해해상국립공원에 유입된 것으로 판단된다. 현재 다도해해상국립공원 남동리 해안에서 분포하고 있는 갯쥐꼬리풀(Spartina alterniflora)군락은 습지내 총 6개의 염생식물 군락면적(8,487.7㎡) 중 75.34%의 비율(6,394.86㎡)로 분포하고 있다. 또한 2008년에 11.54㎡ 이던 분포 면적이 2015년 현재 약 550배가 늘어난 6,394.22㎡로 조사되었고 생육지 고도 범위인 0.69m~1.1m를 요인 값으로 두고 GIS 분석한 하였다. 그 결과 앞으로 확산 될 예상 면적은 12,584㎡이며 현재보다 약 6,189.14㎡가 늘어날 것으로 예측되며 우리나라에서도 역시 확산이 빠르고 넓게 늘어날 것으로 판단된다. 향후 계속 분포역이 확대 될 것으로 예상하고 관리방안을 위하여 시범 연구를 실시하였다. 갯쥐꼬리풀군락 중 일부를 첫 번째는 뿌리째 뽑아내기와 두 번째 지상부 제거 후 포장덮기 방법 등 국외사례의 문헌을 바탕으로 방재를 실시하였으나 전체 면적을 방재하기에는 많은 인력과 시간 그리고 예산이 소요되므로 다각적인 방안을 강구하여야 한다. Spartina alterniflora is native to the Atlantic coast from Newfoundland, the south of Canada and northern Argentina. There it forms a dominant part of brackish coastal saltmarshes. Due to its strong growth, the Spartina alternifolra was introduced to the rider area and coast as a use for ecological engineering. The Spartina alterniflora community can crowd out native species, reduce the biodiversity of saltmarsh and alter the environment. As a result, the invertebrates living in mud flats disappeared due to the encroaching of Spartina alterniflora, and the birds which eat off invertebrates went along as well. The Spartina alterniflora was first introduced in South Korea in 2008 and is known to have flowed in the Dadohaehaesang National Park. Compared to 2008, in 2015 the distribution area of the Spartina alterniflora has increased 550 times. (11.54㎡ in 2008 to 6,394.22㎡ in 2015). The large increase of Spartina alterniflora calls for urgent management and additionary study on the subject. Thus, this study analyzed the geo-spatial information on the Spartina alterniflora such as its distribution area, the height of habitats and expected expansion area, and can act as the preliminary data for management.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

유비쿼터스와 치안서비스

박성수 한국정보사회진흥원 2005 정보화정책 Vol.12 No.4

유비쿼터스 도래와 더불어 국가공공서비스 분야의 하나인 치안서비스와 관련된 많은 논의가 이뤄지고 있다. 특히 위치정보 확인기술이나 생체인식 등과 같은 유망기술은 치안서비스와 관련해 주목받고 있으며 네트워크 사회에 있어 치안서비스를 위한 기초 기술로서 보편화 될 것으로 보고 있다. 특히 현대사회의 여러 가지 부정적인 면과 위험사회를 가정할 때 유비쿼터스 기술을 이용한 공공서비스는 현재 우리가 가지고 있는 방범, 방재, 방화 등의 문제를 해결할 수 있는 효과적 수단으로 주목받고 있다. 그 중 치안서비스 관련정책의 변화와 활성화 효과는 상당히 기대되고 있다. 무엇보다 현대의 흉폭화된 범죄 및 지역사회 범죄 예방에 있어 중요한 역할을 할 것이라 예상된다. 그러나 유비쿼터스 기술을 적용한 치안서비스를 제공함에 있어서 부작용도 고려되어야 할 것이다. 예를 들어 개인의 프라이버시나 개인정보의 공개 등을 들 수 있다. 이는 유비쿼터스 기술을 적용한 치안서비스 제공에 있어서 반드시 해결해야 할 과제라 하겠다. 본 연구에서는 먼저 유비쿼터스 개념과 미래사회의 변화 특히 위험사회 도래에 따른 유비쿼터스 치안서비스의 필요성에 대해 고찰하고 구체적인 서비스분야를 제시해 보았다. 또한 이와 관련하여 제기될 수 있는 문제점을 짚어보았으며 나아가 문제점 극복을 위한 정책을 제언하는데 본 연구의 목적이 있다.