대장-직장암에서 Cyclooxygenase-2의 발현

강원경,천준성,장세진,조현민,천성원,안창혁,오승택 대한대장항문학회 2004 Annals of Coloproctolgy Vol.20 No.2

Cyclophosphamide가 Mouse 肝臟의 燐酸鹽 分解酵素 活性에 미치는 影響

강성준,이군자,정호삼,이규식 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

Cyclophosphamide which is known as a widely used anticancer drug is very effective against a lymphosarcoma, leukemia and nonneoplasmic diseases such as rhematoid arthritis and lupus erythematosus. Cyclophosphamide acts on a DNA as a alkylating agent inhibiting the cell division and growth. And it is also known that metabolites of cyclophosphamide damages the liver cell. The authors have, therefore, undertaken to pursue the effect of cyclophosphamide on the phosphatase activity in the liver. Albino mice, DDY strain, weighing 20gm were used as experimental animals. The experimental animals were administered daily with 200gm of cyclophosphamide per kilogram of body weight were divided into 7-day treatment group and 10-day treatment group. The specimens obtained from the liver were fixed with 10% formalin and sliced at 16 μm thickness in a frozen cryostat. The activity of alkaline phosphatase was observed by the Gomori's method and activity of the adenosine triphosphatase was observed by the Wachstein-Meisel's method for histochemical study. The results were as follows. 1. Alkaline phosphatase activity of the hepatic lobule was moderately positive at the periportal and intermediate zone of the 7-day cyclophosphamide treatment group and trace activity of alkaline phosphatase was observed at the periportal and intermediate zone of the 10-day cyclophosphamide treatment group. 2. Adenosine triphosphatase activity of the hepatic lobule was moderately positive at the periportal, central and intermediate zone of the 7-day cyclophosphamide treatment group and weakly positive at the central and periportal zone of the 10-day cyclophosphamide treatment group.

갑상선에서 Estrogen Receptor의 검색

강성순 연세대학교 원주의과대학 1991 원주의대논문집 Vol.4 No.1

Multiple myeloma 환자에서 Bortezomib치료 후 발생한 마비성 장폐색증 1례

김준영,이원식,손창학,박성길,진한영,박석주,조영완,정은욱,강명주,박정하,주영돈 인제대학교 2006 仁濟醫學 Vol.27 No.-

Bortezomib is the first clinically available proteasome inhibitor that is clinically effective in the treatment of multiple myeloma. A proteasome inhibitor acts through multiple mechanisms to arrest tumor growth, tumor spread, and angiogenesis. The main adverse effects of bortezomib are gastrointestinal symptoms, cytopenia, fatigue, and peripheral neuropathy. To date, severe paralytic ileus has not been reported as a toxic effect of bortezomib treatment in multiple myeloma. Bortezomib is a novel agent that has only been used clinically for 30 months, so a need exists to further evaluate its toxicity. We report a case of grade Ⅲ (NCI CTCAE v3.0) or grade Ⅳ(SWOG toxicity criteria) paralytic ileus in a 65-year-old man with relapsed multiple myeloma who underwent one cycle of single-agent bortezomib treatment scheduled in a 21-day cycle (1.3 mg/㎥ as a single i.v. bolus on days 1, 4, 8, and 11).

대식세포의 Pro-inflammatory cytokine 생산에 대한 세포디짐의 효과

주성수,강희철,원태준,황광우,이도익 중앙대학교 약학연구소 2003 약학 논총 Vol.17 No.-

Cefodizime, a third cephalosporine, was originally developed for use in infectious disease as an advanced antibiotics. Cefodizime has also been known to play a meaningful role in activating natural killer properties of immune cells as well as enhancing immunological parameters such as phagocytosis. Furthermore, as the infection can result in remarkable changes for homeostatic mediators, it is expected that cefodizime may initiate the strong immuno- modualtory effect. In the present work, we investigated whether cefodizime has a biphasic effect of anti-bacteria and immunomodulation via macrophage. For the study, we isolated mouse peritoneal macrophages and treated with cefodizime 10㎍/㎖, 50㎍/㎖, 100㎍/㎖, IFN-v 10U/㎖.+LPS l㎍/㎖ Cells were then incubated for 4, 8, 12, and 24hrs to analysis the expression of IL-1, IL-6, IL-12 mRNA from macrophages. The results showed that cytokines were expressed more than that of control in a time-dependent manner and 100㎍/㎖ cefodizime group showed the greatest expression as much as the positive control (IFN-v+LPS)showed at 24h. In conclusion, cefodizime can play a significant role for the expression of pro-inflammatory cytokines, IL-1, IL-6, and IL-12 in earlier stage so that macrophages and other immune cells can response to the infectious substances, immediately. Moreover, when such pro-inflammatory cytokines are increased, we can also expect that the enhancement of cell-cell interaction of NK cell, CTL and B cell by cefodizime can affect the immunomodulatory effect along with its major role in anti-bacterial effect.

A comparative study of pulp vitality following vital pulpotomy using calcium hydroxide-based sealants in beagle dogs

Gil Joon Jung, Se Eun Kim, Chun-Sik Bae, Kyung Mi Shim, Seok Hwa Choi, Seong Soo Kang1 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4

Vital pulpotomy is a very useful method for disarming of canine tooth, tooth fracture, periodontitis, and malocclusion in veterinary dentistry. Calcium hydroxide is the material commonly used as a liner during vital pulpotomy. This creates a mineralized barrier by stimulating osteoblastic hard tissue repair, arrests the inflammatory response, and soothes dentin. However, the powder or mix type calcium hydroxide materials have many disadvantages due to complicated procedures for use and are hard to handle when vital pulpotomy is followed under general anesthesia in animals. This study was conducted in order to compare the effect of mix and premixed paste type calcium hydroxide as a liner in vital pulpotomy. Six beagle dogs underwent hemisection on the mesial root of the mandibular first molar and vital pulpotomy on the distal root of the first molar. On the distal root of the left and right mandibular first molar, mix type (DYCAL®, Dentsply, USA) and premixed paste type calcium hydroxide (VITAPEX®, Morita, Japan) were used as liners, respectively. Radiological evaluation was performed at immediate, 4, 12, and 20 weeks after vital pulpotomy. According to the results, all teeth had well-formed dentinal bridges, and there were no periradicular lucency, lamina dura loss, or anomalies of the pulp cavity. According to these results, on vital pulpotomy in animals, premixed paste type calcium hydroxide was easy to handle and decreased the anesthesia period due to a more convenient application procedure. A further study of many clinical cases is needed for evaluation of side effects and other problems.

화학 용액 증착 공정으로 제조된 후막 Pb(Zr_(0.52), Ti_(0.48))O₃의 e_(31,f) 특성

박준식,양성준,강성군,나경환 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.9

Thick Pb(Zr_(0.52), Ti_(0.48))O₃(briefly, PZT) films are required for the cases of micro actuators and sensors with high driving force, high breakdown voltage and high sensitivity, and so on. In this work, thick PZT films were fabricated by chemical solution deposition process. Total 16 types of samples using thick PZT films with thicknesses, about 1 ㎛ and 2 ㎛, and Pt top electrodes shapes for measuring effective transverse piezoelectric coefficients (e_(31,f)) were prepared using MEMS processes. All samples were characterized by fabricated e_(31,f) measurement system before and after poling process at 125 kV/cm and 150℃ for 10 min. |e_(31,f)| values of samples after poling were higher than samples before poling. Those of 2 ㎛ thick PZT films were also higher than 1 ㎛ thick PZT films. We found that |e_(31,f)| was directly related with {111} to {h00} crystal structures of thick PZT films. And those with narrow electrodes (x₁- x_(0)) as top Pt electrodes were also higher than cases with wide top Pt electrodes in agreement with given equation.

급성 심근경색후 생존심근 진단에서 Dobutamine 심초음파도와 Thallium 심근스캔의 정확성 비교

이근,강덕현,송재관,이철환,최기준,홍명기,김재중,박성욱,박종훈,박승정 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.2

한국인에서 다변성 D12S391 유전좌의 집단유전학적 연구

이용욱,김성민,구태완,이혜린,강일호,한길로,이혜승,황적준 大韓法醫學會 1999 대한법의학회지 Vol.23 No.1

The hypervariable short tandem repeat(STR) locus D12S391 was investigated in a Korean population. A total of 14 alleles were detected by size under denaturing conditions in 517 unrelated individuals. To confirm all of the alleles detected in a Korean population, a total of 34 fragments were sequenced. Prior to allele designation, we constructed the allelic ladders containing 11 alleles sequenced in this study. Allele 18 is the most common with a frequency of 0.281 in Koreans, and one variant allele 19.3 which have been confirmed by sequencing, was detected. The observed heterozygosity, the power of discrimination (PD), and the mean exclusion chance (MEC) for the locus D12S392 is 0.781, 0.946 and 0.652 , respectively. No deviation from Hardy-Weinberg equilibrium was observed in a Korean population (p=0.557). In the 424 meioses in 105 Korean families confirmed using other 17 STR loci, no mutation was detected in locus D12S391. The STR locus D12S391system is useful both for the analysis identification and parternity.

시설재배 작물의 적정 시비량 결정을 위한 간이 검정법

홍순달,임태준,강성수,양원석 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

토양의 질산태 질소 함량이 67㎎ ㎏-1에서 343 ㎎ ㎏-1의 분포를 갖는 8개 시설재배 토양에서 시비수준을 무비구와 표준시비구로 하여 오이의 생산력을 검정하고 토양 화학성과의 상호관계 및 회귀관계의 평가로부터 질소 적정 시비량 추천모델을 추정하였다. 토양의 질산태 질소 함량과 무비구 오이의 생체중, 비료효과 및 시비효율의 상호관계를 Cate & Nelson 분할법에 의하야 추정한 질소 시비의 상한기준은 280㎎ ㎏-1으로 추정되었다. 토양의 질산태 질소 수준에 따른 표준시비구의 생체중 변화의 관계로부터 토마토 질소 표준시비를 위한 질산태 질소 하한기준은 100㎎ ㎏-1으로 추정되었다. 따라서 상한기준과 하한기준 사이의 질산태 질소 검정에 의한 토마토, 오이, 배추의 질소 시비량 추천모델은 다음과 같이 개발하였다. 질소 표준시비량에 대한 비율 Y=-0.5556X +155.568. 재배기간이 장기간인 오이의 질소 덧거름 시비량 결정방법을 확립하기 위하여 ChlorophyⅡ meter (SPAD502, Minolta)를 활용한 엽록소 검정법을 검토하였다. 평가방법은 NO3-N 함량이 67∼343㎎ ㎏-1인 8개 토양에서 무비구 오이의 생체중과 경시적 엽위별 엽록소 측정치와의 회귀관계로부터 오이의 생산능력과 질소공급능력에 대한 엽록소 측정치의 기준을 추정하였다. 오이 재배기간 동안 엽위별 엽록소 측정치는 정식 후 39일째 13매 잎에서 최대를 보인 후 감소되었으며, 그 이후 오이의 엽록소 측정치는 토양의 질소 공급능력에 따라 현저한 차이를 나타냈다. 정식 후 39일째 13매엽의 엽록소 측정치는 토양의 질산태 질소, 무비구 오이의 생체중 및 질소 흡수량, 시비효율과 유의성 있는 상관관계를 보였다. 그리고 오이 최대 생체중에 대한 부족비율은 13매엽 엽록소 측정치와 유의성 있는 부의 상관을 보였으며 엽록소 최대치에 대한 부족비율과는 곡선적인 정의 상관을 보였다. 따라서 오이 최대 생체중에 대한 부족비율을 덧거름 요구량이라고 가정하면 13매엽의 엽록소 측정치는 덧거름의 사용시기와 기준을 결정할 수 있는 가능성을 보였다. 따라서 시설재배 오이의 밑거름은 질산태 질소의 토양검정에 의하여 결정하고 덧거름은 오이 정식 후 39일째 13매엽의 엽록소 측정으로 결정할 수 있는 종합적인 질소 시비추천 방안을 확립하였다.