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Mitomycin C투여에 의한 공막의 조직병리학적 연구
이군자 서울保健大學 1996 論文集 Vol.16 No.1
익상편 수술 후 재발 방지를 위하여 사용되고 있는 MMC를 안구의 성장이 활발한 어린 흰쥐에게 점안한 후 나타나는 공막의 조직학적 및 조작 화학적인 변화를 관찰하여 다음과 같은 결과를 얻었다. 1) 저농도(0.04mg/mL)의 MMC를 점안한 공막에서는 조직학적인 변화를 관찰할 수 없었으나, 고농도(0.1mg/mL) 의 MMC를 3일간 점안한 실험군과 7일간 점안한 실험군에서는 공막실질의 아교섬유의 간격이 상당히 벌어졌으며 이러한 조직 손상은 MMC 점안을 중지한 후에도 완전히 회복되지 않았다. 2) 고농도(0.lmg/mL)의 MMC를 점안한 실험군의 공막실질에서 alkaline phosphatase 활성은 약간 감소하였으며, MMC 투여 후 7일이 경과한 경우에도 효소활성이 회복되지 않았다. 3) 저농도(0.04mg/mL) 및 고농도(0.lmg/mL)는 MMC를 점안한 실험군의 공막실질에서 섬유모세포와 제I형 아교질 항체에 대한 반응은 대조군에 비하여 감소하였으며, MMC 점안 후 7일이 경과한 실험군에서도 제I형 아교질항체에 대한 반응은 회복되지 않았다. 4) 저농도의 MMC를 1회 점안한 실험군, 3일간 점안한 실험군, 접안 후 7일 경과군 및 고농도의 MMC를 1회 투여한 실험군의 공막실질에서는 Cu, Zn-SOD 활성이 관찰되었으나, 고농도의 MMC를 장기간 점안한 실험군과 점안 후 7일이 경과한 실험군에서는 활성이 관찰되지 않았다. 이상과 같은 결과로 MMC를 점안한 흰쥐의 공막실질에서는 일시적으로 Cu, Zn-SOD 의 활성이 증가하여 MMC에 의한 조직독성을 일부 줄일 수 있을 것으로 생각되나, MMC를 계속 점안한 경우에는 alkaline phosphatase 활성이 감소하고 아교섬유의 합성능력이 감소하여 공막실질의 아교섬유가 정상적인 판(lamella) 을 형성하지 못하여 공막괴사 등의 부작용이 나타나는 것으로 생각되며, 고농도의 MMC를 점안한 경우에는 점안을 중지한 후에도 공막에 조직독성이 나타나는 것으로 생각된다. Mitomycin C(MMC), widely used as an adjunct in the surgical treatment of pterygium, was instilled to the young rats to evaluate the histologic and histopathologic toxicity of the sclera. 1. Significant histologic changes were not detected in the lower dose of MMC(0.04mg/mL) treated groups, but lamella structure of sclera was irregular in the higher dose of MMC(0.1mg/ mL) treated groups. These structural changes were not recovered within 7days after ceasation of MMC instillation. 2. Alkaline phosphatase activity was decreased in the higher dose of MMC treated groups, and it was not recovered within 7 days after ceasation of MMC instillation. 3. Type I collagen and type I collagen synthetic activity of fibroblast were decreased in the lower and higher dose of MMC treated groups, and these changes were not recovered within 7 days after ceasation of MMC instillation. 4. Cu, Zn-SOD activity was increased in the lower dose of MMC treated groups and single application of higher dose of MMC, but Cu, Zn-SOD activity was not detected in the other higher dose of MMC treated groups. These results indicate that increase of Cu, Zn-SOD activity may reduce the MMC toxicity temporarily, but repeated MMC treatment changes the sclera irregular by decreasing biosynthesis of collagen in the fibroblast. These irregular lamella structure may lead to necrotizing scleritis.
李君子 서울大學校保健大學院 1975 公衆保健雜誌 Vol.12 No.2
This study was aimed to disclose the magnitudes and trends of deaths and injuries from motor vehicle accidents with the relations to several factors such as population, number of vehicles, roads's conditions and size of area. Raw data were collected from the various available sources of local government. Main data related to incidence, deaths and injuries were obtained from the records of Department of Police in Gyung-gi Province for 4 years period from 1971 to 1974. The following was summary of the results made with the analysis of the data collected: 1. The total events of motor vehicle accidents in Gyung-gi area for 4 years were 18,140 cases. The annual average rates of incidence, death and njury per 100,000 population were 124.5, 13.6 and 160.6 repectively. The above rates were higher in the urban area than in the rural area. On the other hand, the rates of incidence, death and injury tend to decrease annually. 2. The age specific rates of deaths and injuries per 100,000 population were 10.0 and 79.2, respcetively in the ages under 14, whereas 15.8 and 209.8, respectively in the ages above 15. Among total numbers of death and injured, the age group of under 14 constituted 27.7% and 18.6%, respectively. 3. The everage incidence of motor vehicle accidents per 1,000 vehicles registered was 297.7 events and the number of the death and the injured were 32.4 and 383.5 persons per 1,000 vehicles, respectively. 4. The average incidence, death and injury per 100,000 meters of road's length were 105.1 cases, 11.5 and 135.6 persons, respectively. The above averages were higher in the urban than in the rural areas. 5. The average incidence, death and injury per 100km²of area were 41.4 cases, 4.5 and 53.4 persons, respectively. These averages also were larger in the urban than in the rural areas. 6. Setting the values in 1971 as baseline index 100, population, number of vehicles and population density were steadily increased, but both of events and rates per population of incidence, death and injury were decreased in cases and rate per 100,000 population, and there were no definite changes in road's length and size of area. 7. The average numbers of the death and the injured from motor vehicle accidents were 109.0 and 1,289 persons per 1,000 accidents, respectively. On the other hand death versus injury was average 1:11.8. 8. The daily average of motor vehicle accidents in incidence was 12.4 events, that of death and injured were 1.4 and 16.0 persons, respectively. It Showed that the number of accidents was the greatest in July and August. 9. There were no definite differences in the incidence of motor vehicle accidents by the week days, but they happened somewhat more on Sunday and Monday. 10. The motor vehicle accidents occurred most frequently in the 12:00-18:00 o'clock by time. 11. The causes of motor vehicle accidents were classified 95.1% of drivers fault, 2.9% of pedestrians faults, 2.0% of poor maintenance.
Mouse 신장으로부터 Ornithine Decarboxylase의 정제
이군자,조영동 한국생화학회 1992 BMB Reports Vol.25 No.5
자성 mouse의 피하층에 testosterone을 투여하여 ornithine decarboxylase의 활성을 증가시킨 후, 신장으로부터 ornithine decarboxylase를 황산암모늄 분별침전, DEAE-sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5'-phosphate agarose affinity chromatography를 이용하여 분리 정제하였다. 이 효소의 분자량은 SDS-polyacrylamide gel electrophoresis, Sephacryl S-200 gel chromatography와 ornithine decarboxylase의 효소활성 억제제인 14C-DFMO 을 효소와 결합시켜 얻은 autoradiography를 이용하여 52,000 dalton인 동일한 subunit으로 구성된 dimer임을 알 수 있었다. 정제된 효소의 ornithine에 대한 K_(m) 값과 V_(max) 값은 각각 167㎛과 5 nmol CO_(2)/h로 나타났고, pyridoxal-5-phosphate에 대한 K_(m) 값과 V_(max) 값은 각각 0.142㎛과 2.4nmol CO_(2)/h로 나타났다. 효소의 최적 pH 는 7.0, 최적 온도는 37℃이었으며, 정제된 ornithine decarboxylase는 polyamines(putrescine, spermidine, spermine)에 의해 효소 활성이 영향을 받지 않았다. 본 실험에서는 ornithine decarboxylase의 분리과정 중에서 ornithine decarboxylase 활성을 안정화시키는 물질을 발견하였다. Ornithine decarbosylase (ODC) was purified to homogenity from testosterone treated mice kidney by ammonium sulfate fractionation, DEAE-Sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5-phosphate-agarose affinity chromatography. The specific activity of ODC was 14,000 U/㎎, and purification fold was 1,180. The enzyme has a Mr. of about 110,000 dalton and is a dimer of subunit Mr. 52,000. The K_(m), for L-ornithine was 167㎛ and for pyridoxamine phosphate, 142 nM. ODC has an optimum pH of 7.0 and optimum temperature of 37℃. The purified enzyme was not inhibited by polyamine (putrescine, spermidine and spermine). During purification process, ODC stabilizing material was found.
최루가스(o-Chlorobenzylidene Malononitrile)가 흰쥐의 각막과 결막에 미치는 영향
이군자 서울보건대학 1995 서울보건대학 부설 한국보건과학연구소 논문집 Vol.2 No.1
Two groups of rats were exposed to the o-chlorobenzylidine malononitrile(CS) being used in the control of riots, and sacrificed to examine histological and histochemical changes of the cornea and conjunctiva. 1. In animals exposed to the CS gas (50mg/min./㎥) for 20min., reversible histological changes were observed in the cornea. Epithelial and endothelial Na,^(+) K^(+) -ATPase activity were reduced, wing cells of the epithelium were transformed to the squamous shape and mitotic activity occurred actively in the basal layer. 2. In animals exposed repeatedly to the CS gas (50mg/min./㎥) for 20min. once a day for 10 days, Na^(+), K^(+)-ATPase, Mg^(2+)-ATPase and alkaline phosphatase activities of the corneal epithelium and endothelium were reduced. And there was epithelial shrinkage, irregular basement membrane and irregularly arranged collagen fibers were also observed in the stroma. 3. In animals exposed to the CS gas (50mg/min./㎥) for 20min., reversible histological changes were observed in the conjunctiva. Mucin secreting activity of the goblet cells was reduced and lymphocytes, eosinophils were infiltrated. 4. In animals exposed repeatedly to the CS gas (50mg/min./㎥) for 20min. once a day for 10 days, irreversible changes were observed. Desquamation with oedema and shrinkage of the epithelial cells, and loss of some basement membrane were observed. Mucin secreting activity of the goblet cells was reduced and lymphocytes, eosinophils were infiltrated significantly. As a result when eyes are temporally exposed to the CS gas reversible degenerative changes, desquamation with oedema of the epithelium and infiltration are produced. And when eyes are repeatedly exposed to the CS gas histopathologic changes such as desquamation with oedema of the epithelium, infiltration, and reduced mucin secreting activity are occurred.