위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

실험동물에서의 내분비계 교란물질인 DEHP, DBP의 생체내 영향

정지윤,이성호,박영석,박병권,김병수,김상기,박창식,강경선,이영순 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. This study investigated the estrogenic effects of phthalates, which are DEHP and DBP, suspected as endocrine disruptors. It is concluded that three daily subcutaneous administration of test chemicals, which are di-ethylhexyl phthalate(DEHP) and di-n-butyl phthalate(DBP), to 9 week-old rodents. The results revealed that DEHP & DBP show similar uterotrophic activity in the rat and the mouse, using subcutaneous administration. DEHP and DBP failed to affect any of these parameters, which are body weight, mammary gland, vaginal and uterus histopathology. but, DBP increased uterus weight to only one rat of 1.0 g/kg group. The results conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.

하시모토 갑상선염과 동반된 갑상선 유두암에서 BRAFV600E의 발현과 임상적 특징

남성진,최영식,박요한,김정훈 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Background: An association between Hashimoto's thyroiditis (HT) and papillary thyroid carcinoma (PTC) is still controversial. Studies to date established the coexistence of HT in 11% to 36% of PTC patients. BRAFV600E mutation was presented 90% with no HT, 64% with HT in PTC patients. Therefore, BRAFV600E mutation is associated with the pathogenesis of PTC arising in HT. The purpose of this study was to identify predictive factors for the coexistence of PTC and HT and to evaluate the association of the BRAFV600E mutation with the clinicopathological features of coexistence of PTC and HT. Methods: A total of 247 patients underwent surgery for thyroid disorders between January 2007 and April 2009. In all, 212 patients was diagnosed as thyroid papillary carcinoma only (PTC group), 33 had coexistence of PTC and HT (PTC+HT group), and 2 had been diagnosed with HT. DNA was extracted from paraffin embedded thyroid tumor specimens taken from 99 patients with PTC, 13 coexistence of PTC and HT, and 2 was HT. The presence of the BRAFV600E mutation was determined by polymerase chain reaction amplification of exon 15 followed by direct sequencing. Results: Among clinicopathological features sex, age, tumor size and capsule invasion was not significant between PTC group and PTC+HT group. Lymph node metastasis and stage was significantly lower in PTC+HT group than PTC group (p=0.044). BRAFV600E mutation was found in 34 of the 99 PTC group (34.3%), 1 of the 13 PTC+HT group and 2 HT show no BRAFV600E mutation. Conclusion: These data demonstrated that the coexistence of PTC and HT was less aggressive than PTC only.

다발성 골수종 환자에서 척추주위 수질외 형질세포종으로 재발한 1례

정소연,장재식,정희철,천우정,이창화,김성욱,나득영,이상권,박건욱 東國大學校醫學硏究所 2001 東國醫學 Vol.8 No.-

다발성 골수종은 면역글로불린을 분비하는 세포의 클론을 만드는 B림프 전구세포의 형질 전환과 그 증식으로 야기되는 종양이다. 다른 형질세포 종양으로는 골 단독의 형질세포종과 수질외 형질세포종이 있다. 이 종양들은 공통된 기원임에도 불구하고 이들 종양사이엔 병리적, 임상적 차이가 있고 종양간의 관련성에 대해서 잘 알려져 있지 않다. 저자들은 화학치료로 부분 관해를 보인 다발성 골수종 환자에서 혈청이나 요의 골수종 단백질의 증가없이 비전형적인 방식으로 다발성 수질외 형질세포종으로 진행된 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 본 증례는 척수 압박을 야기한 척추주위 종괴로 재발된 경우이다. Multiple myeloma is a neoplastic disorder caused by the proliferation of transformed B lymphoid progenitor cell that gives rise to a clone of immunoglobulin-secreting cells. Other plasma cell tumors include solitary plasmacytoma of bone and extramedullary plasmacytoma(EMP). Despite the common origin, there exists pathological and clinical differences between these neoplasms and associations between them are not completely understood. We are reporting a case of multiple myeloma which, after an initial response to chemotherapy, progressed in an atypical manner, with the development of multiple EMP and abscence of protein of serum or urine. Our case is unusual because the patient had a recurrent paravertebral mass that caused compression the spinal cord.

다기능 5축 복합가공기 구조 최적설계 및 동강성 평가

정용민,장성현,황현영,최영휴,하종식 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

This paper describes a structural design. optimization for light weight and minimum compliance using G.A. and a study on dynamic stiffness evaluation of a 5-axes multi-tasking machine tool. Dynamic stiffness is one of important factors regarding to machine tool accuracy. In design optimization, we optimized the thickness of structural member which minimize the compliance and weight of the machine structure under several constraints. Furthermore, dynamic stiffness, in this study, were obtained by using both FEM analysis and an experimental dynamic stiffness test, that is a random excitation test by using a hydraulic exciter. Both the theoretical and experiment analysis results showed good agreement with each other. Finally, some discussion and review from the view point absolute dynamic stiffness were made based on the analysis results.

소각비산재의 재활용을 위한 물리․화학적 특성

박상찬,조중식 忠州大學校 2007 한국교통대학교 논문집 Vol.42 No.-

Fly ashes generated from solid waste incinerator has heavy metal high contents, and this is classified as hazardous wastes. So environmental problems may happen due to the potential toxicity of hazardous elements released from fly ashes. Accordingly this work have showed the possible uses on by-products after removing pollutants. For the characteristics of fly ashes, the particle size distribution, specific surface area, thermal gravimetric, chemical analysis and SEM are analyzed.

신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

충주시 생활폐기물의 물리·화학적 특성 및 발열량 산정

박상찬,조중식 忠州大學校 2009 한국교통대학교 논문집 Vol.44 No.-

Physico-chemical characteristics of municipal solid waste(MSW) are very important factors for managing MSW. Also these factors are needed to estimate the type and the capacity of MSW treatment facilities. Actually the management of the MSW treatment facilities are often poorly accomplished because the evaluation of the MSW treatment facility have been carried out without considering the data-base for the characteristics on MSW. Hence, it is necessary to measure the characteristics of MSW for establishing the facilities with accurate data-bases about MSW. Analytical investigations were carried out to study physicochemical characteristics and heating values analysis of MSW generated from various sources in Chung-Ju city. Samples were collected at chung-ju city and were conducted for ultimate and proximate analysis. The physical compositions of combustibles of house were 89.7∼94.0% and non-combustibles of house were 6.0∼10.3%, the apparent densities were 204.7∼309.7 kg/㎥. Chemical compositions in houses were C 46.1∼46.7%, H 6.2∼6.4%, O 37.3∼37.7%, N 1.9∼2.0%, S 0.3∼0.4%, Cl 1.2∼1.4% and ash 6.1∼6.3%. Low heating value of the house in chung-ju city showed 1,879∼2,124 kcal/kg and high heating values were 4,504∼4,541kcal/kg.

만성두통환자에 대한 동서협진의 임상적 연구

정인태,이상훈,김수영,차남현,김건식,이두익,이재동,임사비나,이윤호,최도영 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : Because the cause and etiology of chronic headache are not yet fully explained, the treatment of this symptom is not simple. In order to study the effectiveness East-West pain treatment on chronic headache, we evaluated its effect of pain alleviation and quality of life improvement on chronic headache patients who were treated with nerve block and acupuncture, and only acupuncture for eight weeks. Methods : 92 clinical experiment participants were gathered and through a questionnaire patients who experienced headache for more than 4 hours a day and more than 15 days per month were qualified as chronic headache patients. The qualified patients were classified into two groups, nerve block and acupuncture group(EW group, n=49) and acupuncture group(E group, n=43). Treatment was applied 2 times a weeks for 8 weeks. The acupoints, GV20, HN23, ST8, HN46, TE17, GB20, LI20, LI11, LI14, ST36, and LR3 were stimulated for 20 minutes. The effects of two groups were analyzed using VAS scores and BPI(Brief Pain Inventory). Results : The VAS and BPI after four weeks of treatment showed significant improvement among two but differences between the three groups was statistically insignificant. The VAS and BPI of the EW group after eight weeks of treatment showed statistically significant improvement compared to the other two groups. Conclusion : The pain treatment for chronic headache might be contributed to improvement of quality of life as well as alleviation of pain of chronic headache patients. It was suggested that the West pain treatment in combination with East pain treatment might be an useful modality to improve the pain and quality of life in chronic headache patients.

만성두통환자에 대한 침치료가 심박변이도에 미치는 영향

정인태,이상훈,김수영,차남현,김건식,이두익,이재동,임사비나,이윤호,최도영 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : The purpose of this study was to assess the effect of acupuncture treatment for chronic headache patients using power spectrum analysis of the heart rate variability(HRV). Methods : 15 clinical experiment participants were gathered and through a questionnaire patients who experienced headache for more than 4 hours a day and more than 15 days per month were qualified as Chronic Headache patients. Treatment was applied 2 times a weeks for 8 weeks. The acupoints, GV20, HN23, ST8, HN46, TE17, GB20, LI20, LI11, LI14, ST36, and LR3 were stimulated for 20 minutes. The effects of acupuncture treatment were analyzed using power spectrum analysis of the HRV, HRV was recorded before and after acupuncture treatment. Results : HRV before and after treatment was compared after 8 weeks of acupuncture treatment. Increase in mean values of SDNN and RMSSD were observed but the increases were not statistically significants. Increase in mean values of TP, LF and HF were observed but, the increase was significant(p<0.05) only in TP. Conclusions : The results suggest that acupuncture treatment on chronic headache patients can increase the activity of autonomic nervous system. Further use of HRV for quantitative analysis of acupuncture treatment on autonomic nervous system related symptoms is suggested.