Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in <i>Arabidopsis</i>

Kim, Ho Soo,Jung, Mi Soon,Lee, Kyunghee,Kim, Kyung Eun,Yoo, Jae Hyuk,Kim, Min Chul,Kim, Doh Hoon,Cho, Moo Je,Chung, Woo Sik Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>

장애아동들의 지역학교내 통합교육에 대한 초·중·고등학교 교사들의 태도 비교 연구

안성우,김미경 한국특수아동학회 2001 특수아동교육연구 Vol.3 No.-

본 연구는 통합교육에 대한 초ㆍ중ㆍ고등학교의 교사들의 태도에 대해 조사하였다. 우리나라에서도 1994년에 개정된 특수교육진흥법 제15조에 의해 통합교육을 장애 아동 교육 정책으로 삼고 있다. 이 새로운 정책은 장애 아동들을 일반 학교에 받을 것인 지 아닌 지 교사들이 더 이상 결정할 수 없게 하였고 일반 학교에서 장애 아동들을 교육하는 것이 선택 사항이 아니게 되었다. 이 정책은 장애 아동들의 교육 뿐만이 아니라 일반 아동들의 교육에도 영향을 미치게 되었다. 그러나 많은 학자들은 이러한 정책의 실행 성공 여부는 교육적 지원 외에도 교사들의 통합교육에 대한 태도에 달려 있다고 지적하고 있다. 따라서 교사들의 태도를 조사하는 것은 통합교육의 성공을 위한 준비 노력으로서 상당히 의의가 있다. 본 연구에서는 초등 교사와 중등 교사들의 태도를 비교하는 것을 또 다른 목적으로 하고 있다. 이는 초등학교와 중고등학교의 교육 현실이 상이한 점 때문으로 태도는 개인이 속한 환경의 영향을 받기 때문이다. 그런데 기존의 선행 연구들은 특수교사와 일반 교사간에는 많은 연구들이 수행되어 있으나 학교급간에는 많은 연구가 수행되어 있지 않다. 따라서 학교급별 교사들의 통합교육에 대한 태도를 조사하는 것은 상당히 의의가 있다. 또 본 연구에서는 교사들의 인구통계학적 변인들이 통합교육에 대한 교사들의 태도에 미치는 영향을 알아 보려고 시도하였다. 그러나 교사들의 특수교육에 대한 지식 정도가 통합교육에 대한 태도에 미치는 영향을 통제하기 위해 교사들이 방학중 특수교육 관련 연수를 이수한 직후에 태도 조사를 실시하였다. 본 연구의 결과는 교사들이 장애 아동들의 통합교육에 대해 전반적으로는 긍정적인 태도를 갖고 있는 것으로 나타났다. 그러나 학교급간에 통합교육의 효과에 대해 상이한 태도를 갖고 있었다. 초등학교 교사들이 더 호의적이었고 중고등학교 교사들이 덜 호의적이었다. 이는 중고등학교의 교육 현실을 반영하는 것이라고 생각되어진다.또 교육적 효과의 하위 영역에서는 교사들이 통합교육이 긍정적인 영향을 미칠 것이라고 생각하는 영역도 있고 아동들의 교육에 부정적인 영향을 미칠 것이라고 생각하는 영역도 있다. 교사들은 아동들의 교과 학습면을 가장 우려하고 있었다. 교사들이 우려하는 점들을 해소할 수 있는 방향으로 교육 정책이 입안되고 실행될 수 있도록 노력하여야 할 것이다. This study investigated teachers' attitudes as measured by responses of teachers from three different levels of school toward integration of children with disabilities. In 1994 a new policy which emphasized the rights of all children to an education in community based regular schools and the consequent expectation that teachers and no choice about whether they would accept children with disabilities into their classrooms had been introduced in this country. This policy has changed teaching environments in community based regular school. In this study, it was found that teachers expressed positive attitudes toward the integration of children disabilities into their classroom. It was also found that the attitudes toward mainstreaming students with disabilities of teachers in elementary schools are more positive than the attitudes of teachers working in either middle school or high school, and that teachers did not view that mainstreaming help non handicapped children in academic area. They viewed that mainstreaming might have a negative effect on an academic achievement of non handicapped students in general. However, it was pointed out that the availability of ancillary staff was seen as a major factor in the more positive attitudes toward the policy.

간경변 환자에서 Oro-cecal Transit Time의 측정

원경준,이준,변유미,조민근,조주연,서정균,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background: Neurotransmitter like substances in the gut have been proposed to contribute to the overall portal systemic encephalopathy (PSE). The source of the ammonia in patients with PSE is mainly the small bowel, as a result of the conversion of glutamine in the diet to glutamate and ammonia. PSE is highly responsive to changes in diet, to antibiotic therapy and to ingestion of nondigestible disaccharides such as lactulose. This study was performed to evaluate the relationship between PSE and oro-cecal transit time. In addition, performed to evaluate the effect of severity, cause, complications of liver cirrhosis on oro-cecal transit time. Methods: Between August 2006 and February 2007, 48 cirrhotic patients consecutively was enrolled in study their oro-cecal transit time after informed consent was obtained. A non-invasive hydrogen breath test was used to study oro-cecal transit time (OCTT). Result: The OCTT were delayed in cirrhotic patients with PSE compared without PSE (123.3 ± 42.7 min vs 170.0 ± 58.3 min, p<0.05). OCTT tended to prolongate further as the damage in the liver got worse, nevertheless the result was statistically meaningless (p<0.111). Neither presence of ascites or cause of PSE had any influence on gastrointestinal transit. Conclusions: Cirrhotic patients with PSE have more delayed OCTT than without PSE. Further research is needed to find out the relationship between OCTT and severity of liver cirrhosis.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

Multiloop Edgewise Arch Wire의 tip back 정도에 따른 응력 분포에 관한 유한요소법적 연구

이영일,차경석,주진원,이진우 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.2

임상에서 다양하게 사용되는 Multiloop Edgewise Arch Wire(MEAW)는 구치부 치아의 치축 직립을 위해 Ⅱ급 고무줄이나 수직고무줄과 함께 사용된다. 이때 tip backbend의 정도는 각 환자의 구치치축의 근심경사의 정도에 따라 가감이 결정되나 이 장치가 실제 치근막에서 어떤 효과를 나타내는지 정확히 알려진 바는 없다. 이에 저자는 MEAW의 역학적 효과를 알아보기 위해 tip back bend의 정도를 변화시킨 후 Ⅱ급 고무줄, 수직고무줄을 적용시켜 유한요소법을 통해 치근막에서 발생되는 응력의 분포와 크기를 정량적으로 비교, 관찰하여 장치의 역계를 이해하고 치료효과를 예측해 보고자 실험을 고안하였다. 발치나 교정치료의 경험이 없는 25세 남자 정상교합자를 대상으로 컴퓨터 단층촬영을 시행하여 얻은 방사선필림을 기초로 하여 유한요소 상악치아모델과, 치근막모델을 제작하였으며 여기에 beam element로 제작된 MEAW의 tip back bend를 5도, 10도, 15도로 달리하여 Ⅱ급 고무줄 및 수직고무줄을 적용시켜 각 치근막에서의 압축, 인장력의 분포와 응력의 크기를 상호 비교한 결과 다음과 같은 결론을 얻었다. 1.MEAW에 tip back bend를 5도, 10도, 15도로 부여하고 Ⅱ급 고무줄이나 수직고무줄을 적용시켰을때 전체 치근막에 나타나는 압축, 인장의 분포는 Ⅱ급 고무줄, 수직고무줄 각각의 경우에서 tip back bend의 정도에 상관없이 유사한 양상을 나타냈다. 2.각 치아의 치근막에 나타난 압축, 인장력의 크기는 5도, 10도의 경우보다 15도 tip back bend 부여시 더 크게 나타났다. 3.제1, 2대구치 원심측 치근막에서는 압축력이 나타났으며 특히 제2대구치 치근막의 압축부위가 제1대구치 치근막 압축부위보다 더 넓고 압축력의 크기도 더 크게 나타났다. 또한tip back bend정도가 클수록 압축력도 크고 압축부위도 더 넓게 나타났다. 4.대구치 치근막의 압축력은 소구치 치근막의 압축력보다 상대적으로 더 작게 나타났다. 5.Ⅱ급 고무줄과 수직고무줄 적용 시를 비교해 보면 수직고무줄 장착시 전치부 치근막에 인장력이 더 넓게, 그 크기는 더 작게 나타났다. 반면에 구치부 치근막에 나타나는 응력의 분포와 크기는 별 차이를 보이지 않았다. 6.전치부 치근막 인장부위에서 인장력은 견치에서 제일 컸다. This study have been carried out to find out the mechnical effect of Multiloop Edgewise Arch Wire(MEAW) making use of the finite element method. The tip back bend of MEAW taken in this analysis is 5˚, 10˚ and 15˚. In addition, Class II or up & down elastic is applied to find out stress distribution and their values in PDL. A adult male of normal occlusion was selected to create the models of teeth and PDL. And the model of MEAW was also created using commercial finite element code (ANSYS version 5.2). The MEAW is forcibly engaged with a class II or up & down elastic, to determine the initial stress generated in PDL. Comparing the compressive and tensile stress at each reference-planes, following results are obtained. 1.When a MEAW of 5˚, 10˚, 15˚ tip back bend was engaged with Class II or up & down elastic, the distribution of compressive, tensile stress in entire PDL is similar in each case. 2.The values of compressive and tensile stress in PDL is higher in 15˚ tip back bend case than in 10˚ or 5˚ tip back bend case. 3.In the distal PDL of 1st and 2nd molar, compressive stress appears. The compressive area is more wide and its values is higher in PDL of 2nd molar than those in 1st molar. The compressive area and its values become more wide and higher according to the increase of the tip back bend. 4.The values of compressive stress are comparatively smaller in PDL of molars than those in premolars. 5.Comparing class II and up & down elastic case, tensile stress values in anterior teeth PDL are smaller and their distribution is more wide in up & down elastic case than class II elastic case. On another hand, there is no difference in distribution and stress values in PDL of posterior teeth between two cases. 6.Comparing the tensile area in PDL of anterior teeth, tensile stress values are maximum in PDL of canine.

Helicobacter pylori가 만성위염과 위종양에 미치는 영향에 관한 면역조직화학적 밑 초미세구조적 연구

문경래,정순봉,이미숙,이미자,서재홍,김만우,박찬국 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : Helicobacter pylori (H. pylori) is accepted as a principal cause of chronic gastritis and an important factor in the pathogenesis of peptic ulcer and gastric malignancy. The aim of our studies is to investigate relationships between H. pylori and gastric epithelial cells. Materials ðods : One hundred and eighty one H. pylori positive cases of gastric biopsy specimens were studied immunohistochemically and ultrastructurally. Results : p53 positive staining exhibited foveolar epithelial hyperplasia in 1 out of 32 cases, regeneration in 2 out of 12 cases, incomplete intestinal metaplasia in 5 out of 41 cases, complete intestinal metaplasia in 3 out of 18 cases, chronic ulcer in 1 out of 9 cases, tubular adenoma in 2 out of 14 cases and intestinal type of adenocarcinoma in 16 out of 21 cases. Ultrastructurally, H. pylori was observed to be in direct contact with the microvilli of the target cells and was demolishing the surface microvilli. The organisms were in close contact with the uncoated cell membrance. Penetration into the gastric cells by a few organisms was associated with marked cell damage and ultimately to cell disintegration. Conclusion : Our results suggest that H. pylori plays a role in the pathogenesis of gastric cancer, leading a relatively slow, long lasting process that induces chronic active gastritis, intestinal metaplasia, dysplasia, and adenocarcinoma of intestinal type.

용가시나무 뿌리의 성분연구

조경열,우미희,김난주 대구효성가톨릭대학교 응용과학연구소 1993 응용과학연구논문집 Vol.2 No.-

Rosa maximowicziana Regel(Rosaceae) is a deciduous, latifoliate shrub growing in most parts of Korea. The sprout of this plant has been used for food, and the fruit for treating thirst. Also, the root of this plant has been used fr rheumatism in a Korean folk medicine. From the roots of this plant, sterol and its flucoside, and t재 triterpenoid saponins were isolated and the structrues were elucidated by chemical and spectroscopic methods. So, these compounds were identified as β-sitosterol, β-sitosterool-3-O-β-D-glucopyranoside, 2α, 3β, 19α-trihydroxyurs-12-ene-28-oic acid 28-O-β-D-glucopyranosyl ester(kajiichigoside F1) and 2α, 3β, 19α-trihydroxyurs-12-ene-28-oic acid 28-O-β-D-glucopyranosyl ester(rosamultin), and were encounted in this plant for the first time.

레진 시멘트를 이용한 레진 파이버 강화 레진포스트의 치근 상아질에 대한 미세인장결합강도

김진우,유미경,이세준,이광원 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.1

Object The purpose of this study were to evaluate the microtensile bond strength of resin fiber reinforced post to radicular dentin using resin cement according to various dentin surface treatment and to observe the interface between post and root dentin under SEM. Material and Method A total 16 extracted human single rooted teeth were used. A lingual access was made using a #245 carbide bur in a high-speed handpiece with copious air water spray. The post space was mechanically enlarged using H-file(up to #60) and Gates Glidden bures(#3). This was followed by refining of the canal space using the calbrating drill set provided in ER Dentinpost(GEBR, BRASSELER GmbH&Co. KG). The 16 teeth were randomly distrubuted into 4 group of 4 teeth. Group 1 teeth had their post space prepared using 10% phosphoric acid as root canal surface treatment agent during 20s. The canal was then rinsed with saline and dried with paper point. Group 2 teeth had their post space prepared using 3% NaOCI as root canal surface treatment agent during 30min. The canal was then rinsed with saline and dried with paper point. Group 3 teeth had their post space prepared using 17% EDTA as root canal surface treatment agent during 1min. The canal was then rinsed with saline and dried with paper point. Group 4 teeth had their post space prepared using 17% EDTA as root canal surface treatment agent during 1 min. After rinsing with saline, the canal was rinced 10ml of 3% NaOCI for 30min. After drying with paper point, the post(ER Dentinpost, GEBR, BRASSELER GmbH&Co. KG) was placed in the treated canals using resin cement. Once the canal was filled with resin cement(Super bond C&B sunmedical co. Ltd.), a lentulo was inserted to the depth of the canal to ensure proper coating of the root canal wall. After 24 hours, acrylic resin blocks(10·15·50mm) were made. The resin block was serially sectioned vertically into stick of 1·1mm. Twenty sticks were prepared from each group. After that, tensile bond strengths for each stick was measured with Microtensile Tester. Failure pattern of the specimen at the interface between post and dentin were observed under SEM. Results 1. Tensile bond strengths(meen±SD) were expressed with ascending order as follows ; group 4, 12.52±6.60 ; group 1, 7.63±5.83 ; group 2, 4.13±2.31 ; group 3, 3.31±1.44. 2. Tensile bond strengths of Group 4 treated with 17% EDTA +3%NaOCI were significant higher than those of group 1, 2 and 3 (p<0.05). 3. Tensile bond strengths of Group 1 treated with 10% phosphoric acid were significant higher than those of group 2 (p<0.05). Tensile bond strengths of Group 4 treated with 17% EDTA +3% NaOCI was significant higher than those of other groups.

알코올 의존 환자의 MMPI 특성 : 임상 척도와 내용 척도를 중심으로

이윤경,오상우,류미경,박민철 圓光大學校 醫科大學 神經精神科學敎室 1999 圓光精神醫學 Vol.15 No.2

본 연구는 군집 분석을 이용하여 알코올 의존 환자의 MMPI 임상척도와 내용 척도의 특성을 알아보았다. 연구 결과, 60명의 남자 알코올 의존 환자들은 2개의 하위 유형으로 분류되었다. 첫 번째 집단은 모든 척도가 T 점수 45 ~ 55 범위에 있는 정상 유형이었고(n=40, 66.6%), 두번째 집단은 MMPI 타당도 척도의 L, F, K 가 역전된 V형이며 임상 척도는 6-8 유형을 보였다(n=20, 33.4%). 내용 척도에서도 두 집단간에 통계적으로 유의미한 차이가 있었는데, 첫 번째 집단은 프로파일이 정상범위에 속했으며, 두 번째 집단은 ANX, FRS, OBS, HEA, BIZ, ANG, HYP, LSE, SOD, TRT 척도의 T 점수가 첫 번째 하위 유형에 비해 의미있게 상승하였다. 본 연구는 MMPI 임상 척도와 내용 척도가 알코올 의존 환자의 임상적인 진단과 연구에 도움이 된다는 것을 시사한다. This study was to investigate the characteristics of MMPI clinical and content scales on patients with alcohol dependence by using cluster analysis. As a result, the 60 male patients with alcohol dependence were classified into two subtypes. The MMPI profile of first subtype was within normal range, 45~55 of T score(n=40, 66.6%), second subtype had the inverted V shape validity scales and 6-8 clinical scale profile(n=20, 33.4%). The content scale was statistically significant difference between two groups. First subtype was within normal range, second subtype had more elevated T score of ANX, FRS, OBS, HEA, BIZ, ANG, HYP, LSE, SOD, and TRT scale. This study suggest that the MMPI clinical and content scale can be helpful and applicable to clinical diagnosis and research in patients with alcohol dependence.