Ca Ionophore, A23187에 의한 30A-5세포의 지방세포 분화 억제

이권행,윤경하,한만덕 순천향대학교 1993 논문집 Vol.16 No.1

지방세포 분화 억제 과정에서 ??의 역할을 알아보기 위해, 30A-5 preadipocyte가 지방세포로 분화되는 과정에 ?? ionophore, A23187을 첨가하여, 30A-5세포의 지방세포 분화도와 acetyl-CoA carboxylase 활성을 검토하였다. 30A-5 preadipocyte는 insulin과 dexamethasone의 존재하에서 성숙된 지방세포로 분화되는데, 세포가 분화되는 과정에서 1 μM의 ?? ionophore, A23187을 가하면 30A-5 의 지방세포로의 분화가 거의 완전히 억제된다. 30A-5 세포가 지방세포로 분화되는 과정에서 비례적으로 증가하는 지방산 합성의 조절효소인 acetyl CoA carboxylase의 합성은 ?? ionophore, A23187처리에 의해 억제하는데, 30A-5 세포의 지방세포 분화억제는 acetyl-CoA carboxylase합성 저하에 따른 결과로 추측된다. ?? ionophore, A23187에 의한 acetyl-CoA carboxylase 의 합성저하는 비교적 장기간에 걸쳐 나타나는 효과로, 이 억제효과는 ??의 세포막이동의 경쟁적 저해제인 ??에 의해 부분적으로 극복된다. 따라서 A23187에 의한 acetyl-CoA carboxylase의 합성저하와 이에 따른 지방세포로의 분화를 억제하는 과정의 signal tranduction 과정에는 ??의 이동이 중요한 역할을 할 것으로 추정된다. To examine inhibitory effect of ?? ionophore, A23187 on adipocyte conversion of the 30A-5 preadipocytes, we checked the degree of differentiation and acetyl-CoA carboxylase activity of 30A-5 cells in the presence and absence of ?? ionophore, A23187. Inhibition of differentiation of 30A-5 preadipocytes into matured adipocytes was almost completely inhibited by ?? ionophore, A23187. Inhibition of differentiation of 30A-5 cells be resulting from decreased synthesis of acetyl-CoA carboxylase, which is regulatory enzyme in de novo fatty acid synthesis, by ?? ionophore, A23187. Decreased synthesis of acetyl-CoA carboxylase by ?? ionophore, A23187 might be one of the long term effect, and this inhibitory effect was partially recovered by ?? which is competitive inhibitor in membrane transport of ??. Therefore we assumed that ?? mobilization may play a important role on signal tranduction process in inhibitory action of 30A-5 preadipocytes into adipocytes.

Refractory Ceramic Fiber와 Rock Wool로 자극한 폐포대식 세포에서 Reactive Oxygen Species 분비와 관련된 세포내 신호변환계

이권행,임영,양경숙,오현숙,김은경,김경아 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : It is known that the high fibrogenecity of particles is connected with their cytotoxicity for macrophages. Although the molecular mechanism leading to fiber-induced fiber-induced cytotoxicity is still not clear, several mechanism have been suggested. The release of reactive oxygen species(ROS) from activated alveolar macrophages(AM) by dust have been suggested as a possible mechanism of particle-induced cell damage. But the mechanism which man-made vitreous fiber(MMVF) induces the production of ROS in AM is still not clear. In this study, we evaluated the relationship between ROS production and lactate dehydrogenase(LDH) release from alveolar treated with refractory ceramic fiber(RF2) or rock wool(RWI) and signal transduction path-way of ROS production in RF2 or RW1 exposed AM. Methods : We investigated LDH release from MMVF-stimulated AM for index of cytotoxicity. To determine what kind of signal transduction pathways are involved in MMVF-stimulated ROS generation, we used some drugs which have an effect on the signal transduction pathway. Results : RF2 and RW1 induced increase of LDH release with dose-dependent manner with RF2 having greater effect than RW1. There was a dose-dependent increase in the production of ROS by RF2 or RW1. At all level of concentration, RF2 induced more ROS production than RW1. Inhibitors of PKC(bisindolylmaleimide), PLC(U73122 and neomycine) and PTK(genistein and erbstatin) suppressed RF2 or RW1-induced ROS production. Conclusion : There was significant correlation between LDH release and ROS production from AM treated with RF2 or RW1. RF2 and RW1 induced ROS generation through protein kinase C(PKC), phospholipase C(PLC) and protein tyrosin kinase(PTK) pathways.

달맞이꽃(Oenothera odorata Jacquin) 뿌리에서 추출한 항균성분의 특성

신성진,권순경,이권행,성낙도,최우영 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.1

An antibacterial component was isolated from the roots of evening primrose (Oenothera odorata Jacquin). The component was purified by repeated preparative TLC of the chloroform-extract with solvent system of toluene(7): ethyl acetate(4) : formic acid(O.8). NMR spectrum indicated that it is a steroid compound containing radical(-C=C-C=0) and aromatic structures. Its antibacterial activity against strains of the genus Streptococcus was relatively high, the MIC value was 50 according to the Hoechst Manual-345, as compared with the other genera such as Staphylococcus and Escherichia.

급성기 주요우울장애 환자에서 Natural Killer T 세포

박이진,이제훈,이권행,한상익,전양환 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3

Objectives : To evaluate an association between depression and altered immunity, we examined peripheral T lymphocyte or natural killer (NK) cell measures plasma ACTH and cortisol using the flow cytometry in acute and unmedicated patients with major depressive disorder (MDD). Methods : Forty-two patients with MDD from the outpatient clinic and forty normal controls from the hospital staff were recruited. We applied Hamilton Rating Scale for Depression (HAM-D) and Hamilton Rating Scale for Anxiety (HAM-A) for depressed subjects. Peripheral T lymphocyte or NK cell measures (CD3, CD4, CD8, or CD56) and plasma hormones (ACTH and cortisol) were obtained from all subjects. Results : There were no statistical differences in CD3, CD4, CD8, or CD56 between the two subjects. The number of CD56 cells negatively correlated with HAM-D scores (r= -0.42, P<0.01), but did not correlate with HAM-A scores in patients with MDD. The number of CD56 cells showed strong negative correlation with CD4/CD8 (r= -0.47, P<0.01) in the control group, but not in the depressed group. Patients with MDD had higher cortisol level than controls within the normal range. Conclusion : The trait of immunological imbalance and HPA axis abnormality were shown in patients with MDD. Especially, the severity of depression, but not the anxiety, could be reflected as decreased number of CD56 (NK T) cells in acute and Unmedicated state.

Duplication 9p Syndrome 1례

이미경,노영래,김영훈,백청순,신미경,이진,장필상,김진택,이권행,황경태 대한소아과학회 2002 小兒科 Vol.45 No.9

Disrtibutional Patterns of Phospholipase C Isozymes in Rat and Rabbit Kidneys

Kweon - Haeng Lee 대한신장학회 1994 추계학술대회 초록집 Vol.1994 No.-

적출관류 토끼 신장기능에서 칼슘의 역할

이권행(Kweon-Haeng Lee),전은의(Eun-Eui Chun),홍경자(Kyoung-Ja Hong),조규철(Kyu-Chul Cho) 대한약리학회 1986 대한약리학잡지 Vol.22 No.2

저자는 적출관류 토끼 신장기능에서 칼슘의 역할과 furosemide의 이뇨작용에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. hydralazine놔 verapamil을 주로 신혈관 저항의 감소로 이뇨작용을 나타냈다. furosemide를 hydralazine 또는 verapamil 병합투여시 뚜렷한 이뇨작용이 나타났으며 두군 사이에 차이는 없었다. quinidine은 신혈관 수축을 일으켰으나 이뇨작용이 나타났으며 furosemide의 이뇨작용을 항진시켰다. 칼슘제외된 관류액으로 관류시 칼슘은 신혈관 수축에도 불구하고 이뇨작용이 나타났으며 furosemide의 이뇨작용을 항진시켰다. quinidine은 칼슘이 제외된 상태에서 신장기능 및 furosemide의 이뇨작용에 영향을 미 치지 못하였으나 calcium 병합투여시 과도한 신혈관 수축으로 항 이뇨작용을 보였다. verapamil은 칼슘이 제외된 상태에서 약간의 이뇨작용을 보였지만 칼슘 및 furosemide의 이뇨작용을 변화시키지 못하였다. 이상의 성적은 적출관류 토끼 신장에서 칼슘, verapamil 및quinidine이 이뇨작용이 있으며 칼슘은 furosemide의 이뇨작용을 항진시킨다. This study was designed to investigate the role of calcium in the function of an isolated perfused rabbit kidney and its effect on the diuretic action of furosemide. The administrations of hydralazine and verapamil produced remarkable diuretic actions mainly by decreasing renal resistance. The administration of furosemide in combination with hydralazine or verapamil produced remarkable diuretic action and there was no difference between the two groups. The administration of quinidine produced a diuretic action in spite of vasoconstriction and potentiated the diuretic action of furosemide. In the calcium-free perfusion medium, the administration of calcium produced a marked diuretic action in spite of vasoconstriction and potentiated significantly the diuretic action of furosemide. The administration of quinidine did not alter renal function and the diuretic action of furosemide, but the combined administration of quinidine and calcium showed antidiuretic effect due to excessive vasoconstriction in the calcium-free perfusion medium. Although the administration of verapamil produced a slight diuretic action in the calcium-free perfusion medium, verapamil did not alter the diuretic action of calcium as well as the diuretic actions of furosemide alone and in combination with calcium. The results of this experiment show that calcium, verapamil and quinidine produced diuretic actions and calcium potentiates the diuretic action of furosemide.

Plasmid pUB110 을 이용한 Bacillus subtilis 의 형질전환

이권행(Kweon Haeng Lee),유주현(Ju Hyun Yu),신원철(Won Cheol Shin),오두환(Doo Hwan Oh) 한국미생물생명공학회 1984 한국미생물·생명공학회지 Vol.12 No.1

한국 전통간장 및 메주 제조공정에 관한 조사 연구

이권행(Kweon-Haeng Lee),김남대(Nam-Dae Kim),유진영(Jin-Young Yoo) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.3

간장제품의 색 및 맛에 대한 표준화와 제조공정의 표준화를 설정하기 위한 기초연구로서 재래식 및 개량식 메주의 제조공정조사, 시판간장 제품의 성분분석 및 지역별ㆍ연령별 기호도 조사에 의한 간장제품의 성분표준을 조사한 결과는 다음과 같다. 재래식 메주의 제조공정은 100% 환대두를 수도수에서 24시간 수침한 뒤 탈수하여 2시간 상압 가열하고 50℃ 정도까지 냉각한 후 10~15mesh로 분쇄된 것을 원하는 형태와 크기로 성형하여 2일간 겉말림시킨 후 볏짚으로 엮어 천청에 매달거나 볏짚을 깔고 위에 쌓기를 반복하여 20~30일간 발효시켜 출곡한다. 개량식 메주의 제조공정은 전체 제품의 50~70%의 탈지대두를 석발 정선하여 110~120%로 살수하여 0.7~1.2㎏/㎠에서 15~30분 정도 가압 증자한 후 40℃ 정도로 냉각한 것과 30~50%의 소맥을 석발 정선하여 소맥볶음기에서 200~250℃로 가열시킨 모래와 혼합하여 3~4할로 분쇄하여 실온에 가깝도록 냉각한 것을 혼합한 것에 0.2~0.3%의 상업용 종곡인 Aspergillus sojae를 첨가 혼합하여 자동제국실에서 11시간 동안 26~27℃로 유지시킨 후 품온을 상승시켜 30~35℃에서 11시간 유지하면서 한두번 손질 후 포자생성 전에 품온을 25℃로 낮추고 성입 40~44시간 후에 출곡한다. 시판간장의 원료중 대두는 재래식 간장에서 환대두, 개량식 간장에서는 탈지대두를 사용하고 있었으며, 소맥은 대부분 개량식 간장에 사용되고 있었으나, 재래식 간장에는 전혀 사용되지 않고 있었다. 감미료로는 설탕, 이성화당, 포도당, 물엿, 감초추출물, 스테비아 등은 주로 개량식 간장에 사용되고 있었으나, 재래식 간장의 경우 거의 사용치 않고 있었다. 캬라멜의 경우 재래식 및 개량식 양조간장에는 사용되지 않고 있었으나, 대부분 혼합간장에서 사용되고 있었다. 합성보존료의 경우 재래식 간장을 제외하고는 대부분 개량식 간장에 사용되고 있었다. 시판간장의 화학성분은 재래식 간장의 경우 염분 20.12~25.42%, 총 질소 0.67~0.91%, 순추출물 9.47~11.20%, 색도 2.34~4.01, pH 4.92~5.12로 나타났는데 비해 개량식 간장의 경우 염분, 총 질소, 순추출물, 색도 및 pH가 각각 15.20~17.19%, 1.25~1.40%, 18.17~21.47%, 5.41~21.12 및 4.51~4.66이었으며 알코올 함량이 2.97~3.12%로 나타났다. 개량식 혼합간장의 경우 그 함량은 각각 14.38~19.28%, 0.85~1.75%, 11.22~32.30%, 2.46~17.04 및 4.37~5.07로 나타났다. 시판 간장제품의 색, 향 및 맛에 대한 관능검사결과, 지역 및 연령에 관계없이 재래식 간장 보다는 개량식 간장을 선호하는 것으로 나타났으며, 이를 토대로 하였을 때의 간장제품의 성분표준 함량은 염도 16.0%, 총 질소 1.40%, 순엑기스 19.97%, 색도 12.98, pH 4.61 및 알코올 2.96%이었다. Meju is a basis for manufacturing Kanjang. Meju was traditionally prepared at home by different types of process depending on the regional area. It is necessary to standardize and simplify the process of Meju-preparation for Kanjang of good quality. For these purposes, the process of Meju and Kanjang making as well as analysis of commercial Kanjang, were compared. Generally, traditional Meju was prepared by steeping and dehulling the whole soybean. After steeping for 24hr. soybean absorbed water up to 110~120% of its weight. The soaked soybeans were steamed for 2hr. and cooled to 50℃. Cooked soybeans were crushed down to the size of 10~15 mesh and molded. Molded soybeans were dried for 2 days in the air, hung up by rice straw and fermented for 20~30 days under natural environmental condition. On the other hand, commercial soybean koji was made of defatted soybean. Defatted soybeans were steeped in water and steamed for 15~30min at 0.7~12㎏/㎠. Steamed and defatted soybean was cooled to 40℃. Separately, wheat powder was roasted at 200~300℃ by wheat roaster. Mixture of steamed defatted soybean and roasted wheat powder(5/5 to 7/3) were inoculated with 0.1~0.2% Aspergillus sojae and incubated for 2 days at 30℃ with occasional stirring. Chemical analysis showed that traditional soy sauces contained the following composition: NaCl, 20.12~25.42%; total nitrogen, 0.64~0.91%; pure extract, 9.47~11.20%; color, 2.34~4.01; pH, 4.92~5.12. Commercial products contained: NaCl, 15.20~17.19%; total nitrogen, 1.25~1.40%; pure extract, 18.17~21.47%; color, 5.41~21.12; pH, 4.51~4.66 and ethanol, 2.97~3.12%. Organoleptic test on taste, color and flavor of traditional and commercial soy sauce indicated that most of the consumers prefer commercial products to traditional products. Preferrable formulation of Kanjang based on organoleptic test of soy sauces was assumed as containing: NaCl, 16.0%; total nitrogen, 1.40%; pure extract, 19.97%; color, 12.98; pH, 4.61 and ethanol, 2.96.

Attenuated Renomedulary Phopholipase C Isozyme, PLCδ₁in The Developing Spontaneously Hypertensive Rat

Lee, Kweon-Haeng,Choi, Ji-Woong,Cho, Young-Jin 가톨릭 의과학연구원 1997 가톨릭 의과학연구원 국제학술대회 Vol.1 No.-